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<i>In Vitro</i>Preliminary Evidences on the Antioxidant Properties of Biogenic Amines

Pharmacology & Pharmacy 2013年第9期4卷 696-700页

作者： Roberto Stevanato mariangela bertelle Sabrina Fabris Department of Molecular Sciences and Nanosystems University Ca’ Foscari of Venice Venice Italy Department of Molecular Sciences and Nanosystems University Ca’ Foscari of Venice Venice Italy.

Antioxidant properties of the principal biogenic amines were determined in vitro by four analytical methods—Folin Ciocalteu, DPPH, enzymatic and inhibition of lipid peroxidation—in order to avoid possible measuring-method linked mistakes. Different results are obtained, depending on the parameters that each of them measures. The combination of the data indicates that all examined amines show antioxidant characteristics: in particular, tyramine, serotonin, L-norepinephrine, (-)-epinephrine and dopamine owing to their (poly)phenolic structure too, while aliphatic polyamines-spermine, spermidine, putrescine and cadaverine-histamine, melatonin and tryptamine appear to act specifically on the oxygen-consuming species involved in the lipid peroxidation of polyunsaturated fatty acids.

关键词： Biogenic Amines Antioxidants Lipid Peroxidation DNA Protection Membrane Protection

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Antioxidant Properties and Chemical Composition Relationship of Europeans and Brazilians Propolis

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Pharmacology & Pharmacy 2013年第1期4卷 46-51页

作者： Sabrina Fabris mariangela bertelle Oxana Astafyeva Elena Gregoris Roberta Zangrando Andrea Gambaro Giuseppina Pace Pereira Lima Roberto Stevanato Department of Molecular Sciences and Nanosystems University Ca’Foscari of VeniceVeniceItaly Depatment of Biotechnology and Bioecology Astrakhan State University(ASU)AstrakanRussia Institute for the Dynamics of Environmental Processes CNRUniversity Ca’Foscari of VeniceVeniceItaly Department of Environmental Sciences Informatics and StatisticsUniversity Ca’Foscari of VeniceVeniceItaly Institute of Biosciences Sao Paulo State UniversityBotucatuBrazil

The antioxidant activity of ethanol extracts of propolis, bee glue, of various climate and orographic characteristics, collected from Italy, Brazil and Russia, was evaluated measuring their inhibitory action on peroxidation of linoleic acid, radical scavenging ability towards 2,2’-diphenyl-1-picrylhydrazyl, total phenolic content and reducing capacity by enzymatic and Folin method respectively. Propolis samples were chemically characterized by HPLC-MS/MS in order to find a possible correlation between antioxidant activity and polyphenols composition and quantification. The results obtained indicate that Italian and Russian propolis samples have similar polyphenolic composition and, as a consequence, almost similar antioxidant activity, while Brazilian propolis evidence lower polyphenolic and antioxidant characteristics. Climate and orography reasons of these differences are also suggested.

关键词： Propolis Antioxidant Properties Chemical Composition

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