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New a.enues for the trea.ment of immunothera.0}-resista.t p.ncrea.ic ca.cer

World Journa. of Ga.trointestina. Oncology 2024年第4期16卷 1134-1153页

作者： Luis Guilherme de Oliveira.Silva.Fa.ia. Fellip. Bueno Lemos Ma.cel Silva.Luz Sa.uel Luca.Rocha.pinheiro mariana dos Sa.tos Ca.mon Ga.riel Lima.Correa.Sa.tos Ga.riel reis Rocha.Fa.rício Freire de Melo Instituto Multidiscip.ina. em Sa.de Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil

p.ncrea.ic ca.cer(p.)is cha.a.terized by its extremely a.gressive na.ure a.d ra.ks 14th in the number of new ca.cer ca.es ***,due to its comp.exity,it ra.ks 7th in the list of the most letha. ca.cers *** p.thogenesis of p. involves severa. comp.ex p.ocesses,including fa.ilia. genetic fa.tors a.socia.ed with risk fa.tors such a. obesity,dia.etes mellitus,chronic p.ncrea.itis,a.d *** in genes such a. KRa.,Tp.3,a.d SMa.4 a.e linked to the a.0}ea.a.ce of ma.igna.t cells tha. genera.e p.ncrea.ic lesions a.d,consequently,*** this context,some thera.0}es a.e used for p.,one of which is immunothera.0},which is extremely p.omising in va.ious other typ.s of ca.cer but ha. shown little resp.nse in the trea.ment of p. due to va.ious resista.ce mecha.isms tha. contribute to a.drop.in immunothera.0} *** is therefore clea. tha. the tumor microenvironment(TME)ha. a.huge imp.ct on the resista.ce p.ocess,since cellula. a.d non-cellula. elements crea.e a. immunosup.ressive environment,cha.a.terized by a.dense desmop.a.tic stroma.with ca.cera.socia.ed fibrobla.ts,p.ncrea.ic stella.e cells,extra.ellula. ma.rix,a.d immunosup.ressive *** to this a.e genetic muta.ions in Tp.3 a.d immunosup.ressive fa.tors tha. a.t on T cells,resulting in a.shorta.e of CD8+T cells a.d limited exp.ession of a.tiva.ion ma.kers such a. *** this wa.,finding new stra.egies tha. ma.e it p.ssible to ma.ip.la.e resista.ce mecha.isms is ***,techniques such a. the use of TME modula.ors tha. block recep.ors a.d stroma. molecules tha. genera.e resista.ce,the use of genetic ma.ip.la.ion in sp.cific regions,such a. microRNa.,the modula.ion of extrinsic a.d intrinsic fa.tors a.socia.ed with T cells,a.d,a.ove a.l,thera.0}utic models tha. combine these modula.ion techniques constitute the p.omising future of p. ***,this study a.ms to elucida.e the ma.n mecha.isms of resista.ce to immunothera.0} in p. a.d new wa.s of ma.ip.la.ing thi

关键词： p.ncrea.ic ca.cer Immunothera.0} Resista.ce Tumor microenvironment ma.ip.la.ion Combined immunothera.0}

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Vine p.uning wa.te-ba.ed a.tiva.ed ca.bon for cerium a.d la.tha.um a.sorp.ion from wa.er a.d rea. lea.ha.e

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Journa. of Ra.e Ea.ths 2024年第10期42卷 1960-1968页

作者： Ra.0}a.l Forgea.ini pinheiro a.eja.dro Grimm Kátia.da.Boit Ma.tinello Moha.ma. Rizwa. Kha. Na.sha. a.ma. Luis Felip. Oliveira.Silva.Irineu a.tônio Scha.a.h De Brum Guilherme Luiz Dotto Gla.dson Simões dos reis Resea.ch Group.on a.sorp.ive a.d Ca.a.ytic p.ocess Engineering(ENGEp.C) Federal University of Santa MariaAv.Roraima1000-797105900Santa MariaRSBrazi Dep.rtment of Forest Bioma.eria.s a.d Technology Biomass Technology CentreSwedish University of Agricultural SciencesUmed SE-90183Sweden Universida. De La.Costa Calle 58#55-66080002BarranquillaAtlanticoColombia Dep.rtment of Chemistry College of ScienceKing Saud UniversityRiyadh11451Kingdom of Saudi Arabia Institute of Chemistry Federal University of Rio Grande do Sul90040-060Porto AlegreBrazil

In this study,vine p.uning wa.tes(Vp.)were used a. ra. ma.eria. to develop.a. a.terna.ive a.tiva.ed ca.bon(Vp.-a.)for a.sorbing a.d concentra.ing ra.e ea.th elements cerium(Ce(Ⅲ))a.d la.tha.um(La.Ⅲ))from synthetic a.d rea. lea.ha.e *** Ce a.d La.a.sorp.ion studies eva.ua.ed the effects of Vp.-a. dosa.e,p.,conta.t time,ra.e ea.th initia. concentra.ion,a.d *** Vp.-a. a.sorbent wa. subjected to ma.y p.ysicochemica. cha.a.teriza.ion methods to correla.e a.d understa.d its a.sorp.ive *** cha.a.teriza.ion da.a.indica.e a.ca.bona.eous a.sorbent with a.sp.cific surfa.e a.ea.of 467 m^(2)/*** p.tentia. indica.es a.ma.eria. with a.nega.ively cha.ged surfa.e a. a.p. higher tha. 3.1,which is extremely beneficia. to ca.ions *** both ra.e ea.ths elements(REEs),the a.sorp.ion ca.0}city increa.es with the increa.e of the p.,rea.hing its ma.imum a. p. *** kinetic da.a.a.e well fitted by a.ra.i-fra.tiona. o rder,while the Liu model a.reea.ly fits equilibrium *** ma.imum a.sorp.ion ca.0}cities for Ce(Ⅲ)a.d La.Ⅲ)a.e 48.45 a.d 53.65 mg/g a. 298 K,*** thermodyna.ic studies suggest tha. the a.sorp.ion p.ocess is fa.ora.le,sp.nta.eous,a.d exothermic for both *** filling,surfa.e comp.exa.ion,a.d ion excha.ge a.e the domina.t ***,the Vp.-a. wa. subjected to the recovery of REEs from rea. p.osp.ogyp.um lea.ha.e,a.d it is p.oved tha. it ca. be successfully used to recover REEs in a.rea. p.ocess.

关键词： Vine p.uning wa.tes Susta.na.le ca.bon a.sorbent Ra.e ea.th elements p.osp.ogyp.um lea.ha.e Ion-excha.ge mecha.ism

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Liver tra.sp.a.ta.ion a. a. a.terna.ive for the trea.ment of p.rihila. chola.gioca.cinoma. a.critica. review

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Hep.tobilia.y & p.ncrea.ic Disea.es Interna.iona. 2024年第2期23卷 139-145页

作者： Wellington a.dra.s Fra.cisco Tustumi a.exa.dre Cha.a. Sa.ta.a.Ra.a.l Soa.es Nunes pinheiro Da.iel reis Wa.sberg Lilia.a.Duca.ti Lop.s Rubens Ma.edo a.a.tes Vinicius Rocha.Sa.tos Rodrigo Bronze de Ma.tino Luiz a.gusto Ca.neiro D’a.buquerque Dep.rtment of Ga.troenterology Transplantation UnitUniversidade de São PauloSão PauloBrazil

Ba.kground:p.rihila. chola.gioca.cinoma.p.CCC)is a.disma. *** is no consensus rega.d-ing the best trea.ment for p.tients with unresecta.le *** p.esent review a.med to ga.her the current p.eces of evidence for liver tra.sp.a.ta.ion a.d liver resection a. a.trea.ment for p.CCC a.d to build better guida.ce for clinica. *** sources:The sea.ch wa. conducted in p.bMed,Emba.e,Cochra.e,a.d *** rela.ed references were sea.ched *** criteria.were:rep.rts in English or p.rtuguese litera.ure tha. a.p.tients with confirmed dia.nosis of p.CCC;b)p.tients trea.ed with a.cura.ive intent;c)p.tients with the outcomes of liver resection a.d liver *** rep.rts,reviews,letters,editoria.s,conference a.stra.ts a.d p.p.rs with full-text una.a.la.ility were excluded from the ***:Most of the current litera.ure is ba.ed on observa.iona. retrosp.ctive studies with low gra.es of *** resection ha. better long-term outcomes tha. systemic chemothera.0} or p.llia.ion ther-a.0} a.d liver tra.sp.a.ta.ion is a.good a.terna.ive for selected p.tients with unresecta.le *** ca.dida.es for resection or tra.sp.a.ta.ion should be medica.ly fit a.d free of intra.ep.tic or extra.ep.a.ic *** a.genera. rule,p.tients p.esenting with a.tumor ha.ing a.longitudina. size>3 cm or extending below the cystic duct,lymp. node disea.e,confirmed extra.ep.tic dissemina.ion;intra.p.r-a.ively dia.nosed meta.ta.ic disea.e;a.history of other ma.igna.cies within the la.t five yea.s,a.d did not comp.ete chemora.ia.ion regimen a.d were medica.ly unfit should not be considered for *** of these criteria.should be individua.ly *** tra.sp.a.ta.ion or resection should only be considered in highly exp.rienced hep.tobilia.y centers,a.d a.y decision-ma.ing must be ba.ed on a.multidiscip.ina.y ***:p.CCC is a.comp.ex condition with high *** thera.0}es,including hep.tec-tomy a.d liv

关键词： Liver tra.sp.a.ta.ion Chola.gioca.cinoma Liver neop.a.ms

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Colon a.d recta. ca.cer:a. emergent p.blic hea.th p.oblem

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World Journa. of Ga.troenterology 2024年第7期30卷 644-651页

作者： Ma.ina.pinheiro Da.id Na.cimento Moreira.Michele Ghidini La.V REQUIMTEDepartment of ChemistryFaculty of PharmacyUniversity of Porto4050-313 PortoPortugal Unida.e de Sa.de p.blica Unidade Local de Saúde Barcelos/EsposendeBarcelinhos 4755-069BragaPortugal Medica. Oncology Unit Fondazione IRCCS Ca’Granda Ospedale Maggiore PoliclinicoMilan 20122Italy

Colorecta. ca.cer ra.ks third globa.ly,with a.high morta.ity *** the United Sta.es,a.d different countries in Europ.,orga.ized p.p.la.ion screenings exist a.d include p.op.e between 50 a.d 74 yea.s of *** screenings ha.e a.lowed a. ea.ly dia.nosis a.d consequently a. imp.ovement in hea.th *** a.d recta. ca.cer(CRC)is a.disea.e of p.rticula. interest due to the high globa. burden a.socia.ed with it a.d the role a.tributed to p.evention a.d ea.ly dia.nosis in reducing morbidity a.d *** study is a.review of CRC p.thology a.d includes the most recent scientific evidence rega.ding this p.thology,a. well a. a.dia.nosis of the ep.demiologica. situa.ion of ***,the recommenda.ion from a.p.blic hea.th p.rsp.ctive will be discussed in deta.l ta.ing into a.count the context a.d the most current recommenda.ions.

关键词： Colon a.d recta. neop.a.ia Colon a.d recta. tumor Morta.ity Morbidity

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Immune p.thwa. through endometriosis to ova.ia. ca.cer

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World Journa. of Clinica. Oncology 2024年第4期15卷 496-522页

作者： mariana Sa.tos Ca.mon Fa.ia. Fellip. Bueno Lemos Ma.cel Silva.Luz Sa.uel Luca.Rocha.pinheiro Luis Guilherme de Oliveira.Silva.Ga.riel Lima.Correa.Sa.tos Ga.riel reis Rocha.Fa.rício Freire de Melo Instituto Multidiscip.ina. em Sa.de Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil

Endometriosis is a. estrogen-dep.ndent infla.ma.ory disea.e,defined by the p.esence of functiona. endometria. tissue outside of the uterine *** disea.e is one of the ma.n gynecologica. disea.es,a.fecting a.ound 10%-15%women a.d girls of rep.oductive a.e,being a.common gynecologic *** endometriosis is a.benign disea.e,it sha.es severa. cha.a.teristics with inva.ive *** sup.ort tha. it ha. been linked with a. increa.ed cha.ce of develop.ng endometria. ova.ia. ca.cer,rep.esenting a. ea.lier sta.e of neop.a.tic *** is p.rticula.ly true for women with clea. cell ca.cinoma.low-gra.e serous ca.cinoma.a.d ***,the ca.cinogenic p.thwa.s between both p.thologies rema.n p.orly *** studies suggest a.connection between endometriosis a.d endometriosis-a.socia.ed ova.ia. ca.cers(Ea.Cs)via.p.thwa.s a.socia.ed with oxida.ive stress,infla.ma.ion,a.d *** a.ticle a.ms to review current da.a.on the molecula. events linked to the develop.ent of Ea.Cs from endometriosis,sp.cifica.ly focusing on the comp.ex rela.ionship.between the immune resp.nse to endometriosis a.d ca.cer,including the molecula. mecha.isms a.d their *** recent develop.ents in immunothera.0} a.d their p.tentia. to boost the effectiveness of future trea.ments.

关键词： Ova.ia. neop.a.ms Endometriosis Endometriosis-a.socia.ed ova.ia. ca.cers Immune resp.nse Immunothera.0}

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a.fa.ile stra.egy for tuning the density of surfa.e-gra.ted biomolecules for melt extrusion-ba.ed a.ditive ma.ufa.turing a.0}lica.ions

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Bio-Design a.d Ma.ufa.turing 2024年第3期7卷 277-291页

作者： I.a.O.Beeren G.Dos Sa.tos p.J.Dijkstra.C.Mota.J.Ba.er H.Ferreira.Rui L.reis N.Neves S.Ca.a.ero-Esp.nosa.M.B.Ba.er L.Moroni Dep.rtment of Comp.ex Tissue Regenera.ion MERLN Institute for Technology-Inspired Regenerative MedicineMaastricht University6229 ER MaastrichtThe Netherlands 3B’s Resea.ch Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3B’s-p. Government a.socia.e La.ora.ory 4806-909 Braga/GuimarãesPortugal p.LYMa. University of the Basque Country UPV/EHU20018 Donostia/San SebastiánSpain IKERBa.QUE Basque Foundation for Science48009 BilbaoSpain

Melt extrusion-ba.ed a.ditive ma.ufa.turing(ME-a.)is a.p.omising technique to fa.rica.e p.rous sca.folds for tissue engi-neering ***,most synthetic semicrysta.line p.lymers do not p.ssess the intrinsic biologica. a.tivity required to control cell *** of biomolecules on p.lymeric surfa.es of a. sca.folds enha.ces the bioa.tivity of a.construct;however,there a.e limited stra.egies a.a.la.le to control the surfa.e ***,we rep.rt a.stra.egy to tune the surfa.e density of bioa.tive group. by blending a.low molecula. weight p.ly(ε-ca.0}ola.tone)5k(p.L5k)conta.ning orthogona.ly rea.tive a.ide group. with a. unfunctiona.ized high molecula. weight p.L75k a. different *** p.rous three-dimensiona.(3D)sca.-folds were then fa.rica.ed using a.high weight p.rcenta.e(75 wt.%)of the low molecula. weight p.L *** a.p.oof-of-concep. test,we p.ep.red films of three different ma.s ra.ios of low a.d high molecula. weight p.lymers with a.thermop.ess a.d rea.ted with a. a.kyna.ed fluorescent model comp.und on the surfa.e,yielding a.density of 201-561 p.ol/cm^(2).Subsequently,a.bone morp.ogenetic p.otein 2(BMp.2)-derived p.p.ide wa. gra.ted onto the films comp.ising different blend comp.sitions,a.d the effect of p.p.ide surfa.e density on the osteogenic differentia.ion of huma. mesenchyma. stroma. cells(hMSCs)wa. *** two weeks of culturing in a.ba.ic medium,cells exp.essed higher levels of BMp.recep.or II(BMp.II)on films with the conjuga.ed *** a.dition,we found tha. a.ka.ine p.osp.a.a.e a.tivity wa. only significa.tly enha.ced on films conta.n-ing the highest p.p.ide density(i.e.,561 p.ol/cm^(2)),indica.ing the imp.rta.ce of the surfa.e *** together,these results emp.a.ize tha. the density of surfa.e p.p.ides on cell differentia.ion must be considered a. the cell-ma.eria. ***,we ha.e p.esented a.via.le stra.egy for ME-a. community tha. desires to tune the bulk a.d surfa.e functiona.ity via.blending of(m

关键词： a.ditive ma.ufa.turing Blending Surfa.e functiona.iza.ion Surfa.e density Click chemistry Huma.

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p.osta.e ca.cer temp.ra. a.d regiona. trends in Bra.il

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Oncology Resea.ch 2024年第10期32卷 1565-1573页

作者： MEHRSa.Ja.a.IZa.EH HEVELINE Ra.a.E MOURa.ROESCH FERNa.DO KORKES QUOC DIEN-TRINH LEONa.DO OLIVEIRa.reis UroScience School of Medical SciencesState University of CampinasCampinas13083-872Brazil Division of Urology Faculdade de Medicina do ABCSão Paulo09051-040Brazil Division of Urologica. Surgery a.d Center for Surgery a.d p.blic Hea.th Brigham and Women’s HospitalHarvard Medical SchoolBostonMA 02115USA ImmunOncology Pontifical Catholic University of CampinasPUC-CampinasCampinas13087-571Brazil

Objectives:The Bra.ilia. Unified Hea.th System(Sistema.nico de Sa.de−SUS)is the universa. p.blic hea.thca.e system of Bra.il tha. ma.nta.ns a.na.ionwide da.a.a.e of its *** p.ima.y objective wa. to a.a.yze regiona. a.d temp.ra. trends,while our seconda.y goa. wa. to esta.lish correla.ions between sta.es’hea.th economy sta.us a.d their p.osta.e ca.cer(p.a.***:We a.a.yzed Bra.il’s na.ionwide da.a.on p.osta.e ca.cer(p.a.incidence,morta.ity,a.d ca.e ga.hered between 2013 a.d 2021 by the Informa.ion Technology Dep.rtment of SUS(Da.a.SUS),up.a.ed monthly using the Interna.iona. Cla.sifica.ion of Disea.es(ICD-10)***:In the p.riod,273,933 new ca.es of p.a.a.d 135,336 p.a.dea.hs were rep.rted in men a.ed 50 yea.s or over in *** media. a.nua. p.a.sp.cific incidence ra.e(p.SIR)ra.ged from 14.7 in the Southea.t to 6.9 in the North region a.d the media. a.nua. p.a.sp.cific morta.ity ra.e(p.SMR)ra.ged from 7.7 in the Northea.t to 6.0 in the South region(p.r 10,000 men>50).The media. a.nua. morta.ity to incidence ra.io(MIR)wa. highest in the North(0.88)a.d lowest in the Southea.t region(0.44).There were significa.t regiona. differences in p.a.trea.ment ra.es(p.r new ca.es);the Midwest region ha. the highest media. a.nua. surgery ra.e(0.63)while the North region ha. the highest media. a.nua. systemic thera.0} ra.e(0.75)a.d the lowest ra.ia.ion thera.0} ra.e(0.06).Temp.ra. a.a.ysis of the da.a.showed significa.t cha.ge in a.nua. ra.e trends a.ter the yea. 2018 for p.SIR(coefficient[β]=+3.66,p.0.001),a.y trea.ment(β=−0.06,p.0.016),surgery([SR]β=+0.05,p.0.017)ra.ia.ion thera.0}([RTR]β=−0.06,p.0.005)a.d systemic thera.0}([STR]β=−0.10,p.0.002).a.ter the 2020 p.ndemic,a.nua. p.SIR decrea.ed(β=−2.15,p.0.002)but a.nua. p.SMR,MIR,a.d trea.ment ra.es rema.ned *** studies showed tha. the p.SIR wa. strongly nega.ively correla.ed with STR(p.0.001)a.d p.sitively correla.ed with RTR(p.0.004).MIR wa. p.sitively correla.ed with STR(p.0.001)a.d ne

关键词： p.osta.e ca.cer(p.a.ep.demiology Regiona. disp.rities Temp.ra. trends COVID-19 p.ndemic Screening

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The use of Vis-NIR-SWIR sp.ctroscop. in the p.ediction of soil a.a.la.le ions a.ter a.0}lica.ion of rock p.wder

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Informa.ion p.ocessing in a.riculture 2024年第1期11卷 26-44页

作者： Ma.lon Rodrigues Josia.e Ca.la.a.genta.Everson Ceza. Gla.cio Leboso a.emp.rte a.ra.tes dos Sa.tos ÖnderÖza. a.a.da.Silveira.reis Ma.cos Ra.a.l Na.ni Dep.rtment of a.ronomy State University of MaringáColombo AvenueMaringá87020900Brazil Dep.rtment of p.a.t a.d Soil Sciences Mississippi State UniversityMS 39762USA Interna.iona. a.ricultura. Resea.ch a.d Tra.ning Center Izmir 35660Turkey

Some of the p.oblems a.tributed to tra.itiona. la.ora.ory a.a.yses tha. limit the correct a.sessment of the nutrient contents in the soil a.e time requirements a.d high cost of the soil nutrient *** solve these p.oblems,a.study wa. ca.ried out to eva.ua.e the use of visible,nea.-infra.ed,a.d short-wa.e infra.ed(Vis-NIR-SWIR)sp.ctroscop. in the p.ediction of soil a.a.la.le ions submitted to the a.0}lica.ion of rock *** study wa. ca.ried out on a. a.enosol in p.ra.a.a.City/***(rock p.wders)were a.ra.ged within a.sp.it-p.ot system designed in ra.domized blocks with four *** wa. cultiva.ed for 14 months a.ter the a.0}lica.ion of rock ***,96 soil sa.p.es were collected for mea.uring the p. a.d a.a.la.le ions p.K^(+),Ca.(2+),Mg^(2+),S-SO_(4)^(2-),Si,Cu^(2+),Fe^(2+),Mn^(2+),a.d Zn^(2+)a. well a. sp.ctra. rea.ing through a.Vis-NIR-SWIR sp.ctrora.iometer to p.edict the soil chemica. a.tributes through the p.rtia. lea.t squa.e regression(p.S)*** results showed tha. the elements K^(+),Ca.(2+),Mg^(2+),Cu^(2+),a.d Fe^(2+)could be p.edicted with a.rea.ona.le rightness degree(R^(2)_(p.>0.50,Rp.p.1.40)from sp.ctra. ***,for the a.tributes p.,p.S-SO_(4)^(2-),Si,Mn^(2+),a.d Zn^(2+),there were no sa.isfa.tory models(R^(2)_(p.p.p.1.40).Thus,the a.0}lica.ion of rock p.wder cha.ged the sp.ctra. curves a.d,beca.se of tha.,a.lows the building of p.S models to p.edict the elements K^(+),Ca.(2+),Mg^(2+),Cu^(2+),a.d Fe^(2+).Therefore,Vis-NIR-SWIR sp.ctroscop. is a.p.omising a.terna.ive to the routine a.a.yses of soil fertility since it ha. a.va.ta.es such a. fa.t a.a.ytica. sp.ed,low cost,ea.y to op.ra.e,non-destructive,a.d environmenta.ly friendly,beca.se it does not use ha.mful chemica.s.

关键词： a.terna.ive inp.ts a.a.la.le Ions Multiva.ia.e a.a.ysis Soil Sp.ctroscop.

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Wha. is the effect of Tribulus terrestris on testicula. morp.ology a.d sp.rm p.oduction?a. exp.rimenta. study in the ra. model

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a.ia. Journa. of a.drology 2024年第1期26卷 67-70页

作者： Ma.cello Henrique a.a.jo Da.Silva.Ga.riela.Fa.ia.Buys-Gonca.ves Joa. Henrique Duque reis Estra.a.Fra.cisco Jose Ba.cellos Sa.p.io Diogo Benchimol de Souza Urogenita. Resea.ch Unit Rio de Janeiro State UniversityRio de Janeiro 20551-030RJBrazil

The objective of this study wa. to investiga.e whether trea.ment with Tribulus terrestris(Tt)ha. a.y imp.ct on the testicula. morp.ology a.d function in a.rodent *** ma.e ra.s were divided into a.control group.a.d a.group.receiving 100 mg kg^(-1) body weight of Tt *** 4o da.s of exp.riment,the a.ima.s were submitted to eutha.a.ia.ep.didyma. ta.l sp.rma.ozoa.were collected;a.d sp.rma.ozoa.concentra.ion,motility,a.d via.ility were *** a.dition,testicles were collected a.d p.ocessed for histomorp.ometrica. *** were comp.red using the Student's t-test a.d considered significa.t when p.*** concentra.ion,motility,a.d via.ility showed no difference between the ***,testicula. weight a.d volume,seminiferous tubule dia.eter,tunica.p.op.ia.surfa.e density,seminiferous ep.thelium surfa.e density,a.d intertubula. comp.rtment surfa.e density were sta.istica.ly simila. between the ***,seminiferous ep.thelium height a.d tubula. lumen surfa.e density were a.gmented in a.ima.s trea.ed with *** with Tt does not ca.se a.ma.or imp.ct on testicula. morp.ology,p.omoting only subtle *** difference on sp.rma.ozoa.p.ra.eters wa. observed.

关键词： morp.ometry testicle Tribulus terrestris

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p.ylogeny of Ba.teria.from Steelma.ing Wa.tes a.d Their a.idic Enrichment Cultures

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a.va.ces in Microbiology 2014年第12期4卷 816-828页

作者： mariana p. reis.flaviane a. pinheiro p.trícia.S. Costa.a.a.p.ula.C. Sa.ga.o p.ulo S. a.sis Edma. Cha.tone-Souza.a.dréa.M. a. Na.cimento Dep.rta.ento de Biologia.Gera. Instituto de Ciências Biológicas Universidade Federal de Minas Gerais Belo Horizonte Brazil Dep.rta.ento de Engenha.ia.Meta.úrgica.e de Ma.eria.s Escola de Minas Universidade Federal de Ouro Preto Ouro Preto Brazil

Currently, millions of tons of steel a.e p.oduced worldwide. This ha. become a.serious economic a.d environmenta. cha.lenge beca.se the ores used for steel p.oduction a.e nonrenewa.le resources a.d the p.oduction genera.es huge a.ount of wa.te. In this study, we identified a.d investiga.ed the a.ility of ba.teria.from steelma.ing wa.te with low a.d high zinc concentra.ion to p.omote lea.hing of zinc, when enriched by a.idic (p. 2) culture conditions. The biolea.hing a.sa.s indica.ed remova. of Zn, a. in chemica. lea.hing. Ba.teria. communities from crude a.d enrichment culture wa.tes were cha.a.terized by the 16S rRNa.gene. p.ylogenetic a.a.ysis of the genera.ed clone libra.ies revea.ed p.edomina.ce of p.oteoba.teria.a.d Firmicutes. The a.tino- ba.teria. Ba.teroidetes, Cya.oba.teria. a.d Deinococcus-Thermus p.yla.were a.so encountered. The clones were most closely rela.ed to cultiva.le heterotrop.ic ba.teria. Different genera.were identified including iron redox cycling a.d lea.hing ba.teria.such a. Chromoba.terium, a.romona., Escherichia. Ba.illus, a.d Ochroba.trum. These da.a.a.d significa.t new informa.ion on ba.teria.which survive in extremely a.idic conditions. They a.e dista.tly rela.ed to typ.ca. a.idop.iles resp.nsible for the lea.hing p.ocess, which ma.es them good ca.dida.es for future studies on meta. biolea.hing.

关键词： Ba.teria 16S rRNA Diversity Zinc Steelma.ing Wa.tes

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