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Stable isotopes and body composition in children: History, fundamentals, and clinical applications

Health 2013年第8期5卷 61-68页

作者： Wendell Costa Bila Joel Alves Lamounier Andre Everton de Freitas Valmin Ramos Silva Sylvia Dias Turani Jose Eduardo dutra de Oliveira Graduate Program in Health Sciences Federal University of Sao Joao Del Rei-West Campus CenterDivinopolisBrazil Graduate Program in Child and Teenager Health-Medicine School-UFMG Belo Horizonte College of Health and Human Ecology-FASEH VespasianoBrazil Faculty of Medicine School of Sciences of Santa Casa de Misericordia of VitoriaNossa Senhora da Gloria Children’s HospitalVitoriaBrazil Faculty of Medicine Federal University of Sao Joao Del Rei-West Campus CenterDivinopolisBrazil Faculty of Medicine University of Sao Paulo-Ribeirao PretoBrazil

The aim of this study was to gather information on the use of stable isotopes to measure total body water and body composition scan in children. We selected studies in the last 13 years, in addition to classical studies on the subject, indexed in the database PubMed, LILACS, BVS and SciELO. The body composition was characterized by the amount of bone tissue, muscle and adipose tissue, also including the organs as well as levels of body water. Your knowledge becomes increasingly important in light of the changes that occur in the nutritional status of various types of diseases in frameworks, such as diabetes mellitus, protein energy malnutrition, in cases of obesity and metabolic syndrome. The ability to accurately assess body fat mass especially in children is associated with the importance of effective strategies for prevention and treatment of childhood obesity. Historically, in addition to clinical applications, measurements of total body water were used to determine body composition in nutritional studies. To the knowledge of the body composition, the body water can be measured and used by the ingestion of a dose of labeled water. The measured isotope enrichment is a function of the amount of body water. The method of deuterium is particularly interesting for the assessment of body composition in children, due to its characteristics of collection and analysis.

关键词： Body Water Deuterium Body Composition Doubly Labeled Water Obesity Children

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Systematic review of noninvasive treatments to arrest dentin non-cavitated caries lesions

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World Journal of Clinical Cases 2014年第5期2卷 137-141页

作者： Isauremi Vieira de Assun??o Giovanna de Fátima Alves da Costa Boniek Castillo dutra Borges Department of Dentistry Federal University of Rio Grande do NorteNatal59056-000Brazil

AIM: To systematically review the literature on the efficacy of noninvasive methods of arresting the progression of non-cavitated occlusal carious lesions in ***: The Medline/Pub Med,LILACS,Sci ELO and Scopus databases were searched to identify relevant publications through to November *** clinical trials evaluating the ability of noninvasive methods to arrest the progression of occlusal non-cavitated carious lesions in dentin were ***,data extraction and quality assessment were conducted independently and in ***: Of 167 citations identified,nine full text articles were screened and five were included in the *** papers reported on occlusal fissure sealing using a self-curing glass ionomer(n = 1) or resin-based(n = 4) *** the use of resin-based sealant to obliterate occlusal fissures arrested the progression of non-cavitated occlusal carious lesions in ***: Occlusal fissure sealing with a resin-based sealant may arrest the progression of non-cavitated occlusal dentinal *** clinical trials with longer follow-up times should be performed to increase scientific evidence.

关键词： Dentin Dental caries Fissure sealants Preventive therapy Dental restoration

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大豆作为食物在巴西的应用

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中华预防医学杂志 1980年第3期14卷 185-187页

作者： J.E.dutra de Oliveira 葛可佑巴西圣保罗大学里贝伦普列托医学院中国医学科学院卫生研究所

大豆早在1882年就引入了巴西,但直到近十五年才成为重要农作物。巴西已成为世界上第二个大豆出口国(Gomes,1975)。1965年巴西的大豆产量不足50万吨,到1977年其收获量达1,200万吨。尽管生产迅速增长,但人们食用大豆制品的数量,除大豆油... 详细信息

大豆早在1882年就引入了巴西,但直到近十五年才成为重要农作物。巴西已成为世界上第二个大豆出口国(Gomes,1975)。1965年巴西的大豆产量不足50万吨,到1977年其收获量达1,200万吨。尽管生产迅速增长,但人们食用大豆制品的数量,除大豆油外却一直都很低。豆油是在巴西销售的主要食用油。

关键词：巴西大豆食品

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Reproductive Components of Safflower Genotypes Submitted of Bulk Density Levels in the Brazilian Cerrado

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American Journal of Plant Sciences 2017年第9期8卷 2069-2082页

作者： Juliana Terezinha Sasso Paludo Edna Maria Bonfim-Silva Tonny José Araújo da Silva Maurício dutra Zanotto William Fenner Marcio Koetz Department of Agricultural and Environmental Engineering Institute of Agricultural Sciences and Technology—ICAT Federal University of Mato Grosso—UFMT Cuiabá Brazil Faculty of Agronomic Sciences of Botucatu Paulista State University Júlio de Mesquita Filho—UNESP Botucatu Brazil Faculty of Agronomy and Zootechnic-FAAZ Post Graduating in Tropical Agriculture Federal University of Mato Grosso—UFMT Cuiabá Brazil

Nutrient absorption in crops can decline and their development can be hindered by increased bulk density. This study aimed at assessing the manner in which bulk density levels affect the reproductive structures of the safflower genotypes in the Brazilian Cerrado. The completely randomized design was adopted with four replications for the experiment, which was conducted in a greenhouse using Oxisol collected from 0.0 to 0.2 m depth from the region supporting Cerrado vegetation. The treatments included ten safflower genotypes (PI 237538, PI 248385, PI 250196, PI 301049, PI 305173, PI 305205, PI 306520, PI 306603, PI 560202 and PI 613366) and five bulk density levels (1.0, 1.2, 1.4, 1.6 and 1.8 Mg·m-3). Evaluations were done at 90 days after emergence, in terms of the number, diameter and dry mass of the heads. The data were submitted to the analysis of variance. The means were grouped using the Scott-Knott test at 5% probability. The diameter and dry mass of the chapters were influenced by the mean bulk density of 1.10 Mg·m-3. A notable interaction was evident between the safflower genotypes and bulk density levels for the diameter and dry mass of the head alone, revealing the high degree of genetic variability that environmental changes induce among the genotypes. The PI 250196, PI 301049, PI 305173 and PI 305205 genotypes exhibited greater stability to the bulk density variations compared with the others. Mean bulk density of 1.2 Mg·m-3 was found to impair the development of the reproductive components of the safflower genotypes.

关键词： Carthamus tinctorius L. Bulk Density Oleaginous Crop Safflower Genotypes Physical Attributes of Soil

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Non-Polar Chemical Constituents of Atemoya and Evaluation of the Cytotoxic and Antimicrobial Activity

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Phyton-International Journal of Experimental Botany 2021年第3期90卷 921-931页

作者： Suzana Vieira Rabêlo Fernanda Granja da Silva Oliveira Michele Miriam Calixto de Lira Livia Macedo dutra Adilson Sartoratto Marta Cristina Teixeira Duarte Maria Claudia dos Santos Luciano Maria Francilene Souza Silva Cláudia doÓPessoa Manoel Odorico de Moraes Filho Emmanoel Vilaça Costa Jackson Roberto Guedes da Silva Almeida Post-Graduate Program in Biotechnology(RENORBIO) Federal Rural University of PernambucoRecifePernambuco52171-900Brazil Post-Graduate Program in Biotechnology State University of Feira de SantanaFeira de SantanaBahia44036-900Brazil Center for Studies and Research of Medicinal Plants(NEPLAME) Federal University of San Francisco ValleyPetrolinaPernambuco56304-205Brazil Multidisciplinary Center of Chemical Biological and Agricultural Research(CPQBA)Campinas State UniversityCampinasSão Paulo13083-970Brazil National Laboratory of Experimental Oncology(LabNOE) Federal University of CearáFortalezaCeará60020-181Brazil Departament of Chemistry Federal University of AmazonasManausAmazonas69067-005Brazil

This study aimed to identify the chemical composition of essential oil from fruits(EOAF)and the hexanic crude extract from aerial parts(At-Hex)of atemoya(Annona cherimola x Annona squamosa),a hybrid belonging to the Annonaceae *** and antimicrobial activity was also *** was obtained by hydrodistillation using a Clevenger apparatus,and their composition was analyzed by gas chromatography-mass spectrometry(GC-MS)*** was tested against human tumor cell lines HCT-116(colon carcinoma),SF-295(glioblastoma),OVCAR-8(ovarian carcinoma)and HL60(leukemia)using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT)assay,while antimicrobial activity was conducted by bioauthography method against eleven microorganisms ***-four compounds were identified in the EOAF and twenty-nine in *** monoterpenes linalool(25.70%),α-pinene(10.38%),β-pinene(9.12%),transocimene(7.43%),and the sesquiterpene bicyclogermacrene(12.58%)were the major constituents of EOAF,whereas the sesquiterpene spathulenol(13.91%)was the main compound of ***-Hex showed a high cytotoxicity against SF-295(glioblastoma).These findings show an important chemotaxonomic contribution for the Annonaceae family,mainly for the Annona *** proved to be a promising source of substances with potential cytotoxic activity.

关键词： Annona GC-MS volatile compounds terpenoids biologic activities

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Hesperidin Reduces Cisplatin-lnduced DNA Damage in Bone Marrow Cells of Mice

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Journal of Pharmacy and Pharmacology 2017年第5期5卷 282-288页

作者： Tatiane da Silva Passos Esdras Andrade Santana Maina Mantovanelli da Mota Jean Carlos Vencioneck dutra Juliana Macedo Delarmelina Maria do Carmo Pimentel Batitucci Departamento de Ciencias Biologieas Universidade Federal do Espirito Santo Vitoria 29075-91 Brazil

Hesperidin is a bioflavonoid abundantly found in citrus fruits and displays chemoprotective effects against DNA （deoxyribonucleic acid） damage, however there are few reports about hesperidin effects against cisplatin-DNA damage induction. The aim of this work was to evaluate hesperidin antimutagenicity against cisptatin-DNA damage. （1） The antimutagenicity of hesperidin was assayed by bone marrow of mice in vivo using the micronucleus test. Hesperidin pre-treatment protocol reduced the frequency of MNPCE （micronucleated polychromatic erythrocytes） and the dose of 100 mg·kg-1 was highest efficiency, with 65.24% of damage reduction. In the simultaneous treatment protocol, the dose of 200 mg·kg-1 exhibited a more effective reduction of MNPCE, with 94.01% of damage reduction. （2） Hesperidin was also effective in reducing the MNPCE frequency in the post-treatment protocol for all doses, with 77.48%, 82.13% and 90.08% of damage reduction at the doses of 100, 200 and 400 mg·kg-1, respectively. From the study, it can be concluded that hesperidin was able to promote the reduction of micronuclei frequency and DNA damage induced by cisplatin. Hesperidin is a powerful antioxidant compound and its chemoprotective effects on DNA may occur due to its association with the antioxidant cell system which is responsible for eliminate free radicals generated by chemical harmful to DNA.

关键词： Hesperidin micronucleus test DNA damage chemoprotection antimutagenicity.

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Barriers and Challenges to the Sustainability Requirements Implementation in Public Procurement of Engineering Works and Services

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Open Journal of Civil Engineering 2017年第1期7卷 1-13页

作者： Clézio Thadeu de Souza dutra Ubiratan Rohan Robson Rosa Branco Christine Kowal Chinelli Alexandre Jose Vianna Barbosa de Araujo Carlos Alberto Pereira Soares Graduate Program in Civil Engineering Fluminense Federal University Niterói Brazil

The environmental damage arising from the construction and engineering services was responsible for the appearance of several norms and resolutions regulating and directing the sector’s performance. In this article, we research how professionals with experience in public bids assess the difficulty degree of the implementation of those requirements and how they assess the environmental legislation regarding the protection and conservation of the environment, impact on costs, deadlines and the solution to environmental problems. The results show that industry professionals consider as “high” the level of difficulty to implement the addressed sustainability requirements, and that the Brazilian environmental legislation does not comply with its environmental protection role, increases the possibility of delays and costs of projects and services and hampers the emergence of solutions that could solve environmental problems.

关键词： Environmental Sustainability Requirements Environmental Protection Sustainable Procurement Construction and Engineering Services

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Effect of Ultra-Turrax on Nanocellulose Produced by Acid Hydrolysis and Modified by Nano ZnO by Sol-Gel Method

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Materials Sciences and Applications 2020年第2期11卷 150-166页

作者： Amanda Ramos Aragao Melo Jose Carlos dutra Filho Roberto Pinto Cucinelli Neto Wellington Silva Ferreira Braulio Soares Archanjo Raphael Verdan Curti Maria Ines Bruno Tavares Instituto de Macromoléculas Professora Eloisa Mano Universidade Federal do Rio de JaneiroBl J do Centro de TecnologiaIlh do FundaoRio de JaneiroRJBrasil Instituto SENAI de Inovacao em Biossintéticos e Fibras Universidade Federal do Rio de JaneiroRio de JaneiroBrasil Instituto de Biofísica Carlos Chagas Universidade Federal do Rio de JaneiroRio de JaneiroBrasil Divisao de Metrologia de Materiais Instituto Nacional de MetrologiaQualidade e Tecnologia(INMETRO)Duque de CaxiasBrasil

Cellulose nanocrystals (NCC) and cellulose nanofibrils (CNF) were obtained by a single step process, with synergy between 64% sulfuric acid hydrolysis and high shear from ultra-turrax stirring, which is an advantageous process for disintegrating cellulose microcrystalline and also may improve the hydrolysis process. The surface modification on the cellulose was performed by the sol-gel process, in which the sulfate groups from hydrolysis were replaced by nanoparticles of zinc oxide, which led to the increase of up to 54°C Tonset, according to thermogravimetric analysis (TGA) results. The morphology and crystallinity degree were characterized by Helium Ion Microscopy (HIM), atomic force microscopy (AFM) and X-ray diffraction. In addition, the ZnO band was observed in Fourier transform infrared spectroscopy, furthermore, the change in the zeta potential confirmed the cellulose modification. The changes in the values of proton spin-spin relaxation time for the systems showing the confined hydrogen in the rigid domains, confirmed the results observed with the aforementioned techniques, for both cellulose after hydrolysis and ZnO modified cellulose, suggesting that ZnO disrupted crystal formation in cellulose.

关键词： Cellulose Nanocrystal Cellulose Nanofibril Nano Zinc Oxide Acid Hydrolysis Sol-Gel

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Numerical Simulation of a Liquid Propellant Rocket Motor

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Journal of Thermal Science 2001年第1期10卷 83-86页

作者： Nicolas M.C. Salvador, Marcelo M. Morales, Carlos E.S.S. Migueis, Demetrio Bastos-Netto (INPE - National Institute for Space Research, Rod. Presidente dutra km 40, Cachoeira Paulista, SP, Brazil 12630-000., e-mail:demetrio@yabae,cptec.inpe.br) INPE - National Institute for Space Research Cachoeira Paulista SP Brazil

This work presents a numerical simulation of the flow field in a liquid propellant rocket engine chamber and exit nozzle using techniques to allow the results to be taken as starting points for designing those propulsive systems. This was done using a Finite Volume method simulating the different flow regimes which usually take place in those systems. As the flow field has regions ranging from the low subsonic to the supersonic regimes, the numerical code used, initially developed for commpressible flows only, was modified to work proficiently in the whole velocity range. It is well known that codes have been developed in CFD, for either compressible or incompressible flows, the joint treatment of both together being complex even today, given the small number of. references available in this area. Here an existing code for compressible flow was used and primitive variables, the pressure, the Cartesian components of the velocity and the temperature instead of the conserved variables were introduced in the Euler and Navier-Stokes equations. This was done to penult the treatment at any Mach number. Unstructured meshes with adaptive refinements were employed here. The convective terms were treated with upwind first and second order methods. The numerical stability was kept with artificial dissipation and in the spatial coverage one used a five stage Runge-Kutta scheme for the Fluid Mechanics and the VODE (Value of Ordinary Differential Equations) scheme along with the Chemkin II in the chemical reacting solution. During the development of this code simulating the flow in a rocket engine, comparison tests were made with several different types of internal and external flows, at different velocities, seeking to establish the confidence level of the techniques being used. These comparisons were done with existing theortical results and with other codes already validated and well acceptal by the CFD community.

关键词： numerical ximulation liquid propellant rocket CFD.

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B-1 cells modulate the murine macrophage response to Leishmania major infection

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World Journal of Biological Chemistry 2017年第2期8卷 151-162页

作者： Angelica F Arcanjo Marise P Nunes Elias B Silva-Junior Monique Leandro Juliana dutra Barbosa da Rocha Alexandre Morrot Debora Decote-Ricardo Celio Geraldo Freire-de-Lima Instituto de Biofísica Carlos Chagas Filho Universidade Federal do Rio de Janeiro Instituto Oswaldo Cruz FIOCRUZ Instituto de Microbiologia Paulo de Góes Universidade Federal do Rio de Janeiro Instituto de Veterinária Universidade Federal Rural do Rio de Janeiro

AIM To investigate the modulatory effect of B-1 cells on murine peritoneal macrophages infected with Leishmania major(L. major) in *** Peritoneal macrophages obtained from BALB/c andBALB/c XID mice were infected with L. major and cultured in the presence or absence of B-1 cells obtained from wild-type BALB/c mice. Intracellular amastigotes were counted, and interleukin-10(IL-10) production was quantified in the cellular supernatants using an enzymelinked immunosorbent assay. The levels of the lipid mediator prostaglandin E2(PGE2) were determined using a PGE2 enzyme immunoassay kit(Cayman Chemical, Ann Arbor, MI), and the number of lipid bodies was quantified in the cytoplasm of infected macrophages in the presence and absence of B-1 cells. Culturing the cells with selective PGE2-neutralizing drugs inhibited PGE2 production and confirmed the role of this lipid mediator in IL-10 production. In contrast, we demonstrated that B-1 cells derived from IL-10 KO mice did not favor the intracellular growth of L. *** We report that B-1 cells promote the growth of L. major amastigotes inside peritoneal murine macrophages. We demonstrated that the modulatory effect was independent of physical contact between the cells, suggesting that soluble factor(s) were released into the cultures. We demonstrated in our co-culture system that B-1 cells trigger IL-10 production by L. major-infected macrophages. Furthermore, the increased secretion of IL-10 was attributed to the presence of the lipid mediator PGE2 in supernatants of L. major-infected macrophages. The presence of B-1 cells also favors the production of lipid bodies by infected macrophages. In contrast, we failed to obtain the same effect on parasite replication inside L. major-infected macrophages when the B-1 cells were isolated from IL-10 knockout mice. CONCLUSION Our results show that elevated levels of PGE2 and IL-10 produced by B-1 cells increase L. major growth, as indicated by the number of parasites

关键词： Leishmania major Macrophages B-1 cells Interleukin-10 Prostaglandin E2 Infection

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