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Functional Starter Cultures for Meat: A Case Study on Technological and Probiotic Characterization

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Food and Nutrition Sciences 2015年第5期6卷 511-522页

作者： Antonio Bevilacqua maria rosaria corbo Barbara Speranza Barbara Di Maggio mariangela Gallo Milena Sinigaglia Department of the Science of Agriculture Food and Environment University of Foggia Foggia Italy

The selection of a starter is a complex process, involving different steps, like a preliminary characterization under laboratory conditions, the selection of the most promising strains, a lab validation and the final validation in a large-scale fermentation;the selection of a functional starter includes a further step, dealing with the assessment of the functional traits. This paper proposes a case study on how use a step-by-step approach, based on some rapid protocols, to select promising starter cultures with probiotic abilities. Nine strains, isolated from a commercial preparation, were studied in relation to some enzymatic activities (slime and acetoin production, reduction of nitrates, proteolytic activity and hydrolysis of tributyrin), technological (acidification and growth at different temperatures, pHs and salt amounts) and probiotic traits (hydrophobicity, co-aggregation towards Salmonella sp. and Escherichia coli, survival at pH 2.5 and in presence of bile salts, antibiotic resistance) and compared to some collection isolates. As a final step, a multivariate approach was used to select the most promising isolates. The isolates from the commercial preparation were able to perform the fermentation (e.g. acidification) at low temperatures with some interesting traits in terms of bioactivity towards E. coli O157:H7, whilst some interesting probiotic traits in terms of hydrophobicity and survival in conditions that mimicked the gut were found for the collection isolates.

关键词： Commercial Starter Screening Protocols Technological Performances Functional Traits Multivariate Approach

Bifidobacteria as Potential Functional Starter Cultures: A Case Study by MSc Students in Food Science and Technology (University of Foggia, Southern Italy)

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Food and Nutrition Sciences 2012年第1期3卷 55-63页

作者： Antonio Bevilacqua maria Teresa Cagnazzo Cataldo Caldarola Emanuela Ciuffreda Anna Rita Dragano Stefania Franchino Rossana Lauriola Angela Pacifico maria rosaria corbo Milena Sinigaglia 不详

This research paper was the results of activity of MSc students of Food Science and Technology, attending the class “Biotechnology of Functional Starter”. Five strains of bifidobacteria (Bifidobacterium animalis subsp. lactis;B. longum subsp. infantis;B. breve;B. animalis subsp. animalis;B. bifidum) were evaluated in order to assess their suitability as functional starter cultures, by studying the following technological and probiotic traits: growth at different temperatures, NaCl amounts and pH values;acidifying ability;metabolism (arginin deamination, esculin hydrolysis, acetoin production);survival at low pH and in presence of bile salts;hydrophobic properties;antibiotic resistance. After laboratory assays and strain selection through a multivariate analyses, it was highlighted that B. longum subsp. infantis and B. animalis subsp. lactis represent a good compromise as potential functional starter cultures, as B. animalis subp. lactis showed a high growth index at pH 5 and good values at 25?C and 30?C, as well as the minimal viability loss at pH 2.5. B. longum subsp. infantis DSMZ 20088 was the best microorganism for its growth index in presence of 6.5% of salt added and at 25?C and 30?C.

关键词： Bifidobacteria Functional Starter Probiotic Properties Technological Characterization

Functional Characterization of Bifidobacteria of Human Origin: A Case Study by the Students of Food Science and Technology of the University of Foggia (Southern Italy)

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Food and Nutrition Sciences 2014年第13期5卷 1153-1161页

作者： Emanuela Ciuffreda Angino Veronica Antonietta Cifelli Rossana Foti rosaria Ilenia Forte Fabio Graziani Andrea Giuseppe Longo Angela Longo Valeria Maglia Emilio Francesco Ricciardi Alessandra Sabatino Annamaria Tomaiuolo maria rosaria corbo Milena Sinigaglia Antonio Bevilacqua Department of the Science of Agriculture Food and Environment (SAFE) University of Foggia Foggia Italy

The aim of this paper was to study the potential technological and probiotic properties of bifidobacteria isolated from human feces. Bifidobacteria, naturally present in the dominant colonic microbiota, represent up to 25% of the cultivable faecal bacteria in adults and 80% in infants. Bifidobacteria have been shown to adhere and colonize in high numbers different types of cultured intestinal epithelial cells;moreover some authors reported that some strains are able to stabilize the intestinal microbiota during and after antibiotic therapy, modulate the immune system, protecting against chemically induced intestinal inflammation and reducing symptoms of colitis. Eight isolates of bifidobacteria were studied to assess their technological and probiotic traits;the technological characterization relied on the assessment of enzymatic activities (proteolytic and lipolytic activity), growth under various conditions (pH, temperature and addition of salt), acidifying ability and metabolism (arginine deamination, esculin, esculin hydrolysis and citrate metabolism). The study of the probiotic characteristics focused on the evaluation of the survival at low pH and with bile salts added, antibiotic resistance, and hydrophobic properties. As a result of this process, two promising strains were selected for further studies.

关键词： Bifidobacteria Technology Probiotic Traits Functional Characterization

Iron and Heart Failure: Current Concepts and Emerging Pharmacological Paradigms

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World Journal of Cardiovascular Diseases 2024年第4期14卷 195-216页

作者： maria rosaria De Pascale maria Beatrice Rondinelli Flora Ascione Vincenzo Maffei Chiara Di Lorenzo Sarah Scagliarini Raffaella Faraonio Antonio Faiella Immunohematology and Transfusion Medicine Unit Cardarelli Hospital Naples Italy Immunohematology and Transfusion Medicine Unit San Camillo-Forlanini Hospital Rome Italy Health Management Office Cardarelli Hospital Naples Italy Anesthesiology Unit Cardarelli Hospital Naples Italy Department of Medical and Translational Sciences University of Naples Federico II Naples Italy Oncology Unit Cardarelli Hospital Naples Italy Department of Molecular Medicine and Medical Biotechnologies University of Naples Federico II Naples Italy

Background: Emerging evidence has recognized that anemia and iron deficiency are recurrent comorbidities in chronic heart failure (HF) and several trials have established that iron administration improves myocardial asset and clinical scenario in HF. Purpose: Recent acquisitions suggest that iron deficiency represents a concrete bias in the pathogenetic mechanism of chronic HF, so we have investigated the putative role of the hepcidin/ferroportin axis in the cardiovascular setting to advocate novel pharmacological and clinical approaches. Methods: Here, after an excursus on iron metabolism, we first reviewed the ongoing studies on novel iron targeted compounds. Then, we summarize large clinical interventional studies conducted on patient suffering from iron deficiency and HF which have tested the effects of drugging iron regard QoL, hospitalizations and cardiovascular death. Results: Novel compounds such as hepcidin agonist (PTG 300), synthetic human hepcidin (LJPC-401) and anti FPN (Vamifeport) are ongoing in iron overloaded patients, while the hepcidin blocker (PRS-080) is under investigation in anemic patients. Noteworthy, novel insights could arise from the results of a Phase IV interventional study regarding the modification of hepcidin pathway in a large cohort of HF patients (n = 1992) by sodium glucose cotransporter 2 inhibitors. To date, several studies highlight the beneficial effect of iron administration in cardiovascular setting and latest evidences consider hepcidin level as a novel biomarker of cardiac injury and atherosclerosis. Conclusions: We advocate that data from ongoing studies will suggest novel iron targeted therapies for diagnosis, prognosis and therapy transferable in selected heart failed patients.

关键词： Heart Failure Iron Anemia Iron Deficiency Hepcidin

Potential application of the Kampo medicine goshajinkigan for prevention of chemotherapy-induced peripheral neuropathy

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Journal of Integrative Medicine 2017年第2期15卷 77-87页

作者： Marco Cascella maria rosaria Muzio Division of Anesthesia Department of Anesthesia and Pain Medicine Istituto Nazionale Tumori "Fondazione G. Pascale"--IRCCS via Mariano Semmola Naples 80131 Italy Division of Infantile Neuropsychiatry UOMI-Maternal and Infant Health Asl NA 3 SUD Torre del Greco via Marconi Naples 80059 Italy

Chemotherapy-induced peripheral neuropathy （CIPN） is one of the most common and severe adverse effects related to cancer treatment. Unfortunately, although several agents and protocols have been proposed, no prophylactic strategies have yet to be proven useful. Therefore, new alternative therapies have been considered for CIPN prevention. Herbal medicine in Japan, called Kampo medicine, is derived from traditional Chinese medicine. Goshajinkigan （GJG） is a Kampo medicine, that is comprised of ten herbs. The aim of this work is to analyse the results of pre-clinical and clinical studies on the potential applications of GJG in CIPN prevention.

关键词： chemotherapy peripheral neuropathies herbal medicine alternative medicine natural products

Groups with Cyclic Outer Automizers

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Algebra Colloquium 2014年第3期21卷 381-398页

作者： maria rosaria Celentani Giovanni Cutolo Antonella Leone Dipartimento di Matematica e Applieazioni 'R .Caccioppoli' Universita degli Studi di Napoli 'Federico II' Via Cintia Monte S. Angelo 1-80126 Napoli Italy

We consider groups G such that NG（H）/HCG（H） is cyclic for all H ≤ G. More specifically, we characterise locally nilpotent and supersoluble groups with this property.

关键词： automizers locally nilpotent supersoluble soluble groups

Personality traits and emotional patterns in irritable bowel syndrome

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World Journal of Gastroenterology 2016年第28期22卷 6402-6415页

作者： maria rosaria A Muscatello Antonio Bruno Carmela Mento Gianluca Pandolfo Rocco A Zoccali Psychiatry Unit Department of Biomedical and Dental Sciences and Morphofunctional Imaging University of Messina

The review focuses on those personality traits(neuroticism, extraversion, openness to experience, agreeableness, and conscientiousness), constructs(alexithymia and distressed- Type D personality) and emotional patterns(negative and positive) that are of particular concern in health psychology, with the aim to highlight their potential role on the pathogenesis, onset, symptom clusters, clinical course, and outcome of irritable bowel syndrome(IBS). Personality traits and emotional patterns play key roles in affecting autonomic, immune, inflammatory, and endocrine functions, thus contributing not only to IBS clinical expression and symptomatic burden, but also to disease physiopathology. In this sense, psychological treatments should address those personality traits and emotional features that are constitutive of, and integral to IBS. The biopsychosocial model of illness applied to IBS acknowledges the interaction between biological, psychological, environmental, and social factors in relation to pain and functional disability. A holistic approach to IBS should take into account the heterogeneous nature of the disorder, and differentiate treatments for different types of IBS, also considering the marked individual differences in prevalent personality traits and emotional patterns. Beyond medications, and lifestyle/dietary interventions, psychological and educational treatments may provide the optimal chance of addressing clinical symptoms, comorbid conditions, and quality of life in IBS patients.

关键词： Alexithymia Depression Anxiety Anger Conscientiousness Irritable bowel syndrome Emotions Personality Neuroticism Extraversion

Role of negative affects in pathophysiology and clinical expression of irritable bowel syndrome

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World Journal of Gastroenterology 2014年第24期20卷 7570-7586页

作者： maria rosaria A Muscatello Antonio Bruno Giuseppe Scimeca Gianluca Polfo Rocco A Zoccali Psychiatry Unit Department of NeurosciencesUniversity of Messina98125 MessinaItaly

Irritable bowel syndrome(IBS)is regarded as a multifactorial disease in which alterations in the brain-gut axis signaling play a major *** biopsychosocial model applied to the understanding of IBS pathophysiology assumes that psychosocial factors,interacting with peripheral/central neuroendocrine and immune changes,may induce symptoms of IBS,modulate symptom severity,influence illness experience and quality of life,and affect *** present review focuses on the role of negative affects,including depression,anxiety,and anger,on pathogenesis and clinical expression of *** potential role of the autonomic nervous system,stress-hormone system,and immune system in the pathophysiology of both negative affects and IBS are taken into *** comorbidity and subclinical variations in levels of depression,anxiety,and anger are further discussed in relation to the main pathophysiological and symptomatic correlates of IBS,such as sensorimotor functions,gut microbiota,inflammation/immunity,and symptom reporting.

关键词： Depression Anxiety Anger Mood disorders Irritable bowel syndrome Neuroticism Stress Brain-gut axis Microbiota Hypothalamic-pituitary-adrenal axis

Gallbladder hemorrhage–An uncommon surgical emergency:A case report

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World Journal of Clinical Cases 2022年第27期10卷 9734-9742页

作者： maria rosaria Valenti Andrea Cavallaro maria Di Vita Antonio Zanghi Giovanni Longo Trischitta Alessandro Cappellani Department of Surgery University of Catania Medical SchoolUniversity of CataniaCatania 95123Italy

BACKGROUND Gallbladder hemorrhage is a life-threatening ***(accidental or iatrogenic such as a percutaneous biopsy or cholecystectomy surgery),cholelithiasis,biliary tract parasitosis,vasculitis,vascular malformations,autoimmune and neoplastic diseases and coagulopathies have been described as causes of hemorrhage within the lumen of the *** use of nonsteroidal anti-inflammatory drugs and anticoagulants may represent a risk *** SUMMARY We report the case of a 76-year-old male *** urgent contrast computed tomography scan demonstrated relevant distension of the gallbladder filled with hyperdense non-homogeneous *** gallbladder walls were of regular *** the anterior wall a focus of suspected active bleeding was *** to the progressive decrease in hemoglobin despite three blood transfusions,this was an indication for urgent *** Early diagnosis of this potentially fatal pathology is essential in order to plan a strategy and eventually proceed with urgent surgical treatment.

关键词： Gallbladder Hemorrhage Anticoagulants Cholecystectomy Surgery Case report