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T follicular helper expansion and humoral-mediated rejection are independent of the HVEM/BTLA pathway

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Cellular & Molecular Immunology 2017年第6期14卷 497-510页

作者： Jose-Ignacio Rodriguez-Barbosa Carlos Fernandez-Renedo Ana maria bravo Moral Leo Biihler maria-luisa del Rio Transplantation Immunobiology Section Institute of Biomedicine University of Leon and Castilla and Leon Regional Transplantation Coordination Leon University Hospital Leon Spain Department of Veterinary Clinical Sciences University of Santiago de Compostela Veterinary Faculty Lugo Spain Visceral and Transplantation Surgery Department of Surgery University Hospitals of Geneva and Faculty of Medicine Geneva Switzerland

The molecular pathways contributing to humoral-mediated allograft rejection are poorly defined. In this study, we assessed the role of the herpesvirus entry mediator/B- and T-lymphocyte attenuator （HVEM/BTLA） signalling pathway in the context of antibody-mediated allograft rejection. An experimental setting was designed to elucidate whether the blockade of HVEM/BTLA interactions could modulate de novo induction of host antidonor-specific antibodies during the course of graft rejection. To test this hypothesis, fully allogeneic major histocompatibility complex-mismatched skin grafts were transplanted onto the right flank of recipient mice that were treated with isotype control, anti-CD40L or modulatory antibodies of the HVEM/BTLA signalling pathway. The frequencies of CD4 T follicular helper （Tfh） cells （B220-, CD4＋ CXCR5＋ PD-lhigh）, extrafollicular helper cells （B220-, CD4＋ CXCR5- PD-1＋ and PD-1-） and germinal centre （GC） B cells （B220＋Fas＋ GL7＋） were analysed by flow cytometry in draining and non-draining lymph nodes at day 10 post transplantation during the acute phase of graft rejection. The host antidonor isotype-specific humoral immune response was also assessed. Whereas blockade of the CD40/CD40L pathway was highly effective in preventing the allogeneic humoral immune response, antibody-mediated blockade of the HVEM/BTLA-interacting pathway affected neither the expansion of Tfh cells nor the expansion of GC B cells. Consequently, the course of the host antidonor antibody-mediated response proceeded normally, without detectable evidence of impaired development. In summary, these data indicate that HVEM/BTLA interactions are dispensable for the formation of de novo host antidonor isotype-specific antibodies in transplantation.

关键词： BTLA (CD272) humoral immunity HVEM (TNFRSF14, CD270) T follicular helper cells transplantation

Robot-assisted oncologic pelvic surgery with Hugo™robot-assisted surgery system:A single-center experience

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Asian Journal of Urology 2023年第4期10卷 461-466页

作者： Angelo Territo Alessandro Uleri Andrea Gallioli Josep maria Gaya Paolo Verri Giuseppe Basile Alba Farré Alejandra bravo Alessandro Tedde Óscar Rodríguez Faba Joan Palou Alberto Breda Department of Urology FundacióPuigvertAutonomous University of BarcelonaBarcelonaSpain

Objective To report the outcomes of intra-and extra-peritoneal robot-assisted radical prostatectomy(RARP)and robot-assisted radical cystectomy(RARC)with Hugo™robot-assisted surgery(RAS)system(Medtronic,Minneapolis,MN,USA).Methods Data of twenty patients who underwent RARP and one RARC at our institution between February 2022 and January 2023 were *** primary endpoint of the study was to report the surgical setting of Hugo™RAS system to perform RARP and *** secondary endpoint was to assess the feasibility of RARP and RARC with this novel robotic platform and report the *** Seventeen patients underwent RARP with a transperitoneal approach,and three with an extraperitoneal approach;and one patient underwent RARC with intracorporeal ileal *** intraoperative complications *** docking and console time were 12(interquartile range[IQR]7-16)min and 185(IQR 177-192)min for transperitoneal RARP,15(IQR 12-17)min and 170(IQR 162-185)min for extraperitoneal *** intraoperative complications *** patient submitted to extraperitoneal RARP had a urinary tract infection in the postoperative period that required an antibiotic treatment(Clavien-Dindo Grade 2).In case of transperitoneal RARP,two minor complications occurred(one pelvic hematoma and one urinary tract infection;both Clavien-Dindo Grade 2).Conclusion Hugo™RAS system is a novel promising robotic platform that allows to perform major oncological pelvic *** showed the feasibility of RARP both intra-and extra-peritoneally and RARC with intracorporeal ileal conduit with this novel platform.

关键词： Bladder cancer Prostate cancer Radical cystectomy Radical prostatectomy Robotic surgery

Pain assessment and management in the newborn:A systematized review

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World Journal of Clinical Cases 2021年第21期9卷 5921-5931页

作者： maria Teresa Garcia-Rodriguez Sergio Bujan-bravo Rocio Seijo-Bestilleiro Cristina Gonzalez-Martin Research in Nursing and Health Care Institute of Biomedical Research of A Coruña(INIBIC)Complexo Hospitalario Universitario de A Coruña(CHUAC)SERGASAs Xubias 8415006 A Coruña Universidade da Coruña(UDC)A Coruña 15006Spain Health Sciences Complexo Hospitalario Universitario de A CoruñaA Coruña 15006Spain

BACKGROUND Neonatal pain has been underdiagnosed due to several false *** To determine the status of neonatal pain in newborns who are admitted to intensive care *** Different databases were *** reviews and research reports conducted in newborns that were written in English,Spanish,or Portuguese,published between 2010 and 2020,and having free access to the full text were selected.A total of 135 articles were found,and 18 articles were finally *** Newborns are exposed to numerous painful *** order to assess their pain levels,several scales have been used,although they are sometimes not correctly *** terms of pain management,the nursing team plays a very important role based mainly on both pharmacological and non-pharmacological *** Nursing staff members must be well trained in order to identify pain and to interpret the scales ***,they have an important role in performing non-pharmacological procedures for pain management.

关键词： Neonatal pain Intensive care unit Nursing Management Treatment Systematized review

Single Layer Centrifugation with Androcoll-E^(TM) improved progressive motility and percentage of live spermatozoa with intact acrosome of chilled stallion semen but did not have an effect on DNA integrity

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Open Journal of Animal Sciences 2012年第3期2卷 159-165页

作者： Ana luisa Costa Ana Martins-Bessa Ana Rebello de Andrade Tiago Guimaraes maria Rosa Rebordao Sandra Gamboa Pedro Pinto bravo maria José Correia Jorge Colaco Isabel Gaivao António Rocha Centro de Ciencia Animal e Veterinária(CECAV) University of Trás-os-Montes e Alto DouroQuinta de PradosVila RealPortugal Veterinary Degree Universty of Tras-os-Montes e Alto DouroQuinta de PradosVila RealPortugal Centro de Estudos em Ciencia Animal(CECA) Instituto de Ciencias Biomedicas Abel Salazar(ICBAS)University of PortoOportoPortugal Coimbra Higher School of Agriculture Sao Martinho do BispoCoimbraPortugal Alter Real Foundation Alter do ChaoPortugal

A significant number of stallions produce low quality ejaculates with high sensibility to chilling. Single Layer Centrifugation (SLC) with Andro-coll-ETM has been presented as an efficient method of selecting good quality spermatozoa. In the current study, changes in sperm quality (motility, viability, acrosome integrity and DNA damage) occurring during storage at 5?C for a maximum of 72 h, were investigated. For that, one ejaculate from 12 stallions was split in two aliquots: control and SLC-selected. Both aliquots were chilled and stored at 5?C and spermatozoa were evaluated for motility, viability and acrosome integrity at 24, 48 and 72 h post collection. DNA damage was evaluated at 48 h post collection using the comet assay. In the SLC-selected aliquots, there was a significant improvement in terms of progressive motility (0 h: P = 0.005;24 h: P 0.05). SLC with Androcoll-ETM improved semen quality prolonging sperm longevity of chilled semen (P = 0.012). This positive effect was more evident in ejaculates most sensitive to chilling that had a sharp decrease in motility in the first 24 h of refrigeration and for all ejaculates at 72 h post-chilling. Therefore, this method reveals to be a useful technique for selecting spermatozoa and maintain sperm quality during storage.

关键词： Stallion Sperm Single Layer Centrifugation Androcoll-ETM Membrane Integrity Comet assay Component Formatting Style Styling

The Use of the ICTs in the Value Chain Proce,;ses and Their Impact on Organizational Performance

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Chinese Business Review 2016年第9期15卷 437-446页

作者： Zulema Cordova Ruiz Sosima Carrillo Loreto maria bravo Zanoguera Jessica Lizbeth Cisneros Martinez Autonomus University of Baja California Mexicali Mexico

Extramedullary plasmocytoma associated with a massive deposit of amyloid in the duodenum

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世界胃肠病学杂志：英文版 2009年第28期15卷 3565-3568页

作者： Fabiana Pirani Carneiro maria de Nazareth Machado Sobreira Lívia bravo Maia Alesso Cervantes Sartorelli Luiz Eduardo de Almeida Prado Franceschi Mauro Brito Brando Bárbara Wosnjuk Calaca Fernando Silva Lustosa Joo Vieira Lopes Department of Pathology University Hospital of Brasília Department of Radiology University Hospital of Brasília Department of Hematology University Hospital of Brasília Department of Surgery University Hospital of Brasília

We report a rare case of extramedullary plasmocytoma associated with a massive deposit of amyloid in the duodenum.A 72-year-old Japanese man was admitted to our hospital presenting with a 3-mo history of epigastric pain, vomiting and weight *** computed tomography(CT) a wall thickening of the fourth part of the duodenum was *** biopsies obtained from the lesion showed inflltration of plasma cells and lymphocytes, but they were not *** patient underwent resection of the lesion and, on histopathological examination, the lesion consisted of a dense and diffuse inflltrate of plasma cells and a few admixed lymphocytes with reactive follicles extending to the muscular *** extensive deposition of amyloid was also *** stains revealed that a few plasmacytoid cells showed λ light chain staining, though most were κ light chain *** cells also were positive for CD138 and CD56 but negative for CD20 and *** flndings were consistent with extramedullary plasmocytomaassociated with a massive deposit of amyloid in duodenum.A subsequent workup for multiple myeloma was completely *** patient showed no signs of local recurrence or dissemination of the disease after 12 mo *** of the association of plasmocytoma and amyloidosis, the patient must be followed up because of the possible systemic involvement of the neoplasm and amyloidosis in future.

关键词：淀粉样蛋白十二指肠浆细胞存款免疫组织化学染色计算机断层扫描组织病理学检查淀粉样变性

Characterization Of Peripherical Blood And Tumor Infiltrating Lymphocytes In Cervical Carcinoma Patients By Flow Cytometry

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Characterization Of Peripherical Blood And Tumor Infiltratin...

Correction to: T follicular helper expansion and humoralmediated rejection are independent of the HVEM/BTLA pathway

24th International Papillomavirus Conference and Clinical Workshop

作者： maria Angelica Cespedes Ardila maria Mercedes bravo Hernandez Susana Fiorentino Alba Lucia Combita Rojas Instituto Nacional de Cancerologia Instituto Nacional de Cancerologia Pontificia Universidad Javeriana Instituto Nacional de Cancerologia

＜正＞Decreases of host immune function,alterations in frequencies of lymphocyte subsets and increase of regulatory T cells（T reg ）have been observed in patients with different types of *** cervical cancer,the changes of immune responses in tumor milieu,especially the composition of TILs,may have important implications in terms of the progression of *** characterize the tumor infiltrating lymphocytes and peripheral blood lymphocytes in patients with cervical cancer,we analyzed 39 peripheral

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来源： cnki会议评论

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Cellular & Molecular Immunology 2019年第3期16卷 314-314页

作者： Jose-Ignacio Rodriguez-Barbosa Carlos Fernandez-Renedo Ana María bravo Moral Leo Bühler maria-luisa del Rio Transplantation Immunobiology Section Institute of BiomedicineUniversity of Leon and Castilla and Leon Regional Transplantation CoordinationLeon University HospitalLeonSpain Department of Veterinary Clinical Sciences University of Santiago de CompostelaVeterinary FacultyLugoSpain Visceral and Transplantation Surgery Department of SurgeryUniversity Hospitals of Geneva and Faculty of MedicineGenevaSwitzerland

In this article,one of the grating agencies requested us to incorporate the information,Spanish Government and co-funded by European Union ERDF/ESF,“Investing in your future”,in the acknowledgments *** correct acknowledgement is as follows:“This work has been supported by grants of the Spanish Ministry of Health(Fondo de Investigaciones Sanitarias,PI13/00029,Spanish Government and co-funded by European Union ERDF/ESF,“Investing in your future”),Department of Education of Castilla and Leon Regional Government(Grant#LE093U13)and Mutua Madrileña Foundation(Basic research grants 2012)to J.I.R.B.;by Miguel Servet National Program(Ministry of National Health)CP12/03063 and by Gerencia Regional de Salud GRS963/A/2014 to *** are particularly grateful to *** Alaiz for outstanding animal husbandry.”The authors regret the errors.

关键词： section. expansion husbandry

Clinical impact of confocal laser endomicroscopy in the management of gastrointestinal lesions with an uncertain diagnosis

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World Journal of Gastrointestinal Endoscopy 2017年第8期9卷 389-395页

作者： Carlos Robles-Medranda maria Vargas Jesenia Ospina Miguel Puga-Tejada Manuel Valero Miguel Soria Gladys bravo Carlos Robles-Jara Hannah Pitanga Lukashok Gastroenterology and Endoscopy Division Instituto Ecuatoriano de Enfermedades DigestivasUniversity Hospital OMNIGuayaquil 090505Ecuador

AIM To evaluate the clinical impact of confocal laser endomicroscopy(CLE) in the diagnosis and management of patients with an uncertain *** A retrospective chart review was *** who underwent CLE between November 2013 and October 2015 and exhibited a poor correlation between endoscopic and histological findings were *** characteristics,indications,previous diagnostic studies,findings at the time of CLE,clinical management and histological results were *** based on CLE findings were also *** compared the diagnostic accuracy of CLE and target biopsies of surgical *** A total of 144 patients were *** these,51%(74/144) were *** mean age was 51 years *** all,41/144(28.4%) lesions were neoplastic(13 bile duct,10 gastric,8 esophageal,6 colonic,1 duodenal,1 rectal,1 ampulloma and 1 pancreatic).The sensitivity,specificity,positive predictive value,negative predictive value,and observed agreement when CLE was used to detect N-lesions were 85.37%,87.38%,72.92%,93.75% and 86.81%,***'s Kappa was 69.20%,thus indicating good *** in management were observed in 54% of the *** CLE is a new diagnostic tool that has a significant clinical impact on the diagnosis and treatment of patients with uncertain diagnosis.

关键词：共焦的激光 endomicroscopy 在 vivo 显微镜学小型帽子食管胃肠的癌症胰腺的包囊胆汁的苛评