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In vitro induced pluripotency from urine-derived cells in porcine

World Journal of Stem Cells 2022年第3期14卷 231-244页

作者： Kaiana Recchia Lucas Simões Machado Ramon Cesar Botigelli Naira Caroline Godoy Pieri Gabriela Barbosa Raquel Vasconcelos Guimarães de Castro mariana Groke Marques Laís Vicari de Figueiredo Pessôa Paulo Fantinato Neto Flávio Vieira Meirelles Aline fernanda de Souza Simone maria Massami Kitamura Martins Fabiana Fernandes Bressan Department of Surgery Faculty of Veterinary Medicine and Animal SciencesUniversity of São PauloPirassununga 13635-000São PauloBrazil Department of Pharmacology and Biotechnology Institute of BioscienceSão Paulo State UniversityBotucatu 18618-689São PauloBrazil Department of Veterinary Medicine Faculty of Animal Sciences and Food EngineeringUniversity of São PauloPirassununga 13635-000São PauloBrazil Embrapa Suínos e Aves Empresa Brasileira de Pesquisa AgropecuáriaConcordia 89715-899Santa CatarinaBrazil Department of Animal Sciences Faculty of Animal Sciences and Food EngineeringUniversity of São PauloPirassununga 13635-000São PauloBrazil

BACKGROUND The generation of induced pluripotent stem cells(iPSC)has been a game-changer in translational and regenerative medicine;however,their large-scale applicability is still hampered by the scarcity of accessible,safe,and reproducible *** porcine model is a large biomedical model that enables translational applications,including gene editing,long term in vivo and offspring analysis;therefore,suitable for both medicine and animal *** To reprogramme in vitro into pluripotency,and herein urine-derived cells(UDCs)were isolated from porcine *** The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4(OCT4),SRY-box2(SOX2),Kruppel-like factor 4(KLF4),and C-MYC,and cultured with basic fibroblast growth factor(bFGF)*** characterize the putative porcine iPSCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase(AP),OCT4,SOX2,NANOG,TRA181 and SSEA 1 *** transcripts related to the pluripotency(OCT4,SOX2 and NANOG)were analyzed via reverse transcription quantitative realtime polymerase chain reaction in different time points during the culture,and all three lineages formed embryoid bodies(EBs)when cultured in suspension without bFGF *** The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro *** of putative iPSCs were obtained only from UDCs transduced with the murine factors(mOSKM),but not from human factors(hOSKM).Three clonal lineages were isolated and further cultured for at least 28 passages,all the lineages were positive for AP detection,the OCT4,SOX2,NANOG markers,albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and SSEA1,similar results were observed in the abundance of the endogenous transcripts related to pluripotent *** the clonal lineages when cultured in suspension without

关键词： Induced pluripotent stem cells Noninvasive Pluripotency Reprogramming Urine Porcine

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CXCL9 and CXCL10 display an agedependent profile in Chagas patients:a cohort study of aging in Bambui,Brazil

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Infectious Diseases of Poverty 2020年第3期9卷 132-132页

作者： fernanda Fortes de Araújo Karen Cecília Lima Torres Sérgio Viana Peixoto Antonio Luiz Pinho Ribeiro Juliana Vaz Melo Mambrini Vitor Bortolo Rezende maria Luiza Lima Silva Antônio Ignácio Loyola Filho Andréa Teixeira-Carvalho maria fernanda Lima-Costa Olindo Assis Martins-Filho Integrated Research Group in Biomarkers Rene Rachou InstituteOswaldo Cruz FoundationAvenida Augusto de Lima1715–Barro Preto–Belo HorizonteMinas Gerais 30190-002Brazil 不详

Background:Chagas disease is endemic in Latin America and still represents an important public health problem in the *** cardiomyopathy is the most significant chronic form due to its association with morbidity and *** last decade has seen increasing evidence that inflammatory cytokines and chemokines are responsible for the generation of inflammatory infiltrate and tissue damage,with chronic chagasic cardiomyopathy patients presenting a pro-inflammatory immune *** studies have evaluated the role of chemokines in experimental *** infection,few have addressed their systemic profile,especially for human infection and in aging *** present work aimed to use the data from a large population based study of older adults,conducted in an endemic area for Chagas disease,to examine the association between serum levels of cytokines and chemokines,*** infection and electrocardiogram(ECG)***:The present work evaluated serum levels of CCL2,CXCL9,CXCL10,CCL5,CXCL8,IL-1β,IL-6,TNF,IL-12 and IL-10 by Flow Cytometric Bead Array assay(CBA)and the results expressed in pg/*** baseline survey started in January 1st 1997,with 1284 participants of an aged population-based *** signed an informed consent at baseline and at each subsequent visit and authorized death certificate and medical records ***:Our results demonstrated that Chagas disease patients had higher serum levels of CXCL9,CXCL10 and IL-1βand lower serum levels of CCL5 than non-infected ***,our data demonstrated that CXCL9 and CXCL10 increased in an age-dependent profile in Chagas disease ***:Together,this study provided evidences that serum biomarkers increase along the age continuum and may have potential implications for establishing clinical management protocols and therapeutic intervention in Chagas disease patients.

关键词： Chagas disease Immune biomarkers Chemokines Cytokines Cohort

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Three-Dimensional Reconstruction of Live Fish through the Moire Technique

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Journal of Agricultural Science and Technology(B) 2018年第4期8卷 242-246页

作者： Ariovaldo Jose da Silva Juliana Aparecida Fracarolli fernanda Fernandes Adimari Pavarin Katia CristinaSuzigan Thais Queiroz Zorzeto Cesar Inacio maria Dal Fabbro School of Agricultural Engineering University of Campinas (UNICAMP) Av. Cdndido Rondon 501 Campinas 13083-875 SP Brazil

Currently, in fish farms, the controls of the physical characteristics of the fishes, for example, size and mass are made by means of the operation of fish removal, in which the tanks are emptied to capture the animals and perform the biometry. This operation demands large volumes of water and generates effluent containing high concentrations of organic matter and nutrients that can contribute to the deterioration of water quality in the recipient bodies. Therefore, the development of technologies that use digital image processing, such as the moird technique and image analysis, can be important allies for the preservation of environmental quality by avoiding the fish removal and the discharge of effluents, increasing productivity due to optimization of the time and still the saving of water. To obtain the images a 9-liter glass aquarium, a support for notebook and light projector, a digital camera brand Samsung Galaxy Camera 2 were used. The objective of this work was to obtain the three-dimensional reconstruction of live fish in aquariums. In the future, the technique can be developed to obtain the mass and the volume of the fish in fish tanks, replacing the fish removal, allowing the preservation of water resources.

关键词： Oreochromis niloticus fish removal water saving image analysis biometrics.

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Massive Vesical Hernias and Its Management: Case Series and Literature Review

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Open Journal of Urology 2022年第3期12卷 193-202页

作者： Adolfo Castro-Rosas Juan Carlos Angulo-Lozano Nezahualcoyotl Gonzaga-Carlos maria fernanda Virgen-Rivera Mariel Gonzalez-Calatayud Jose Francisco Virgen-Gutiérrez Jorge Jaspersen-Gastelum Urology Department of Mexico General Hospital “Dr. Eduardo Liceaga” Mexico City Mexico School of Medicine Anahuac University Mexico City Mexico

Background: Bladder hernias are infrequent and should be treated by the urologist, or an experienced surgeon. An inguinoscrotal hernia of the bladder should be suspected in male patients with voiding symptoms and a fluctuant mass in the scrotum that changes its volume during urination. The most common characteristics found on the series were patients with advanced age, sedentary, and overweight. Case Presentation: We present a case series of 4 patients with inguinoscrotal herniation of the bladder, the surgical management, and outcome. Conclusion: The surgical technique used was inguinal plasty with Lichtenstein technique and either laparoscopic or open surgery mesh-plug placement. None had surgical complications, and none had recurrence at their follow-up.

关键词： Inguinal Plasty Bladder Herniation Lichtenstein Technique Functional Urology

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Delivery of essential pediatric congenital surgical care within Brazil’s universal health coverage system:a national survey of pediatric surgeons

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World Journal of Pediatric Surgery 2023年第3期6卷 136-146页

作者： Paul R Truche Abbie E Naus Fabio Botelho Julia Ferreira Alexis Bowder Luke Caddell Kathrin Zimmerman Isabella maria de Freitas Faria Bellisa Caldas Lopes Eduardo Corrêa Costa fernanda Lage Lima Dantas Augusto J S A Cavalcante Carlos A L B Carvalho Simone Abib David P Mooney Nivaldo Alonso Program in Global Surgery and Social Change Harvard Medical SchoolBostonMassachusettsUSA Department of Surgery Rutgers Robert Wood Johnson Medical SchoolNew BrunswickNew JerseyUSA Department of Pediatric Surgery McGill UniversityMontrealQuébecCanada Department of Surgery Medical College of WisconsinMilwaukeeWisconsinUSA Division of Otolaryngology-Head and Neck Surgery Department of SurgeryUniversity of Wisconsin-Madison School of Medicine and Public HealthMadisonWisconsinUSA School of Medicine UFMGBelo HorizonteMinas GeraisBrazil Department of Pediatric Surgery Instituto de Medicina Integral Professor Fernando FigueiraRecifeBrazil Department of Pediatric Surgery Hospital de Clinicas de Porto AlegrePorto AlegreBrazil Department of Pediatric Surgery Federal University of AcreRio BrancoBrazil Department of Pediatric Surgery Hospital Santa Casa de Belo HorizonteBelo HorizonteBrazil Department of Pediatric Surgery Federal University of Mato GrossoCuiabaBrazil Department of Pediatric Surgery UNIFESPSao PauloBrazil Department of Pediatric Surgery Boston Children's HospitalBostonMassachusettsUSA Department of Plastic Surgery University of São Paulo Institute of Biomedical SciencesSao PauloBrazil

Objective In this study,we assess the delivery of congenital pediatric surgical care under Brazil’s system of universal health coverage and evaluate differences in delivery between public and private *** A cross-sectional national survey of pediatric surgeons in Brazil was *** were asked which of 23 interventions identified through the Disease Control Priorities 3(Surgical Interventions for Congenital Anomalies)they perform and to report barriers faced while providing surgical *** were weighted by state and stratified by sector(public vs private).Results A sample of 352 responses was obtained and weighted to represent 1378 practicing pediatric surgeons registered in Brazil during the survey time.73%spend the majority of their time working in the public sector(‘SistemaÚnico de Saúde’and Foundation hospitals),and most of them also work in the private ***,Brazilian pediatric surgeons have the expertise to provide thoracic,abdominal,and urologic *** working mostly in the public sector were more likely to report a lack of access to essential medications(25%vs 9%,p<0.01)and a lack of access to hospital beds for surgical patients(52%vs 32%,p<0.01).Conclusions Brazilian pediatric surgeons routinely perform thoracic,abdominal,and urologic *** working in government-financed hospitals face barriers related to infrastructure,which may impact Brazilians who rely on Brazil’s universal health coverage *** that support pediatric surgeons working in the public sector may promote the workforce available to provide congenital pediatric surgical care.

关键词： pediatric survey Brazil

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Association between Neck Circumference and Glycemia in Adolescents

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Journal of Diabetes Mellitus 2018年第3期8卷 61-69页

作者： Cláudia Rucco Penteado Detregiachi Karina Quesada Sandra maria Barbalho Deisiane de Cassia Mulato Mirela Moris Tassiana Minardi Vitoria Esborini Nogueira fernanda Fioravanti Bernardo Department of Biochemistry and Nutrition University of Marília (UNIMAR) S&atildeo Paulo Brazil Department of Biochemistry and Nutrition Faculty of Food Technology of Marília (FATEC) S&atildeo Paulo Brazil

Background: The determination of glycemia is an essential element for the diagnosis of diabetes or possible glycemic alterations. The body composition and fat distribution are related to this parameter. The neck circumference (NC) is an anthropometric measure that has been used to evaluate the overweight and accumulation of subcutaneous fat in the upper body, regardless of age. It is also a useful tool to predict insulin resistance and other risk factors. This research aimed to study the relationship between the NC and glycemia in adolescents and, thus, to analyze the applicability of this anthropometric measure in the screening of glycemic alterations in this group. Methods: The study population consisted of 600 adolescents aged 10 to 19 years. Data on sex, birth date, NC, fasting blood glucose and stage of sexual maturation was collected. Results: The evolution of sexual maturation generated a significant difference in the NC in both sexes, but not affected glycemia. Thus, the association between NC and glycemia was investigated according to this parameter. Among the pre-pubertal adolescents, the NC was significantly higher among those who had glycemia within borderline values, and the opposite occurred among pubertal adolescents. Among post-pubertal adolescents, NC was similar between the two groups. A negative correlation was found in all adolescents, as well as among those who were in the pubertal and post-pubertal stages of sexual maturation. Differently, adolescents in the pre-pubertal stage showed a positive correlation between the NC and the biochemical parameters. Conclusion: This study showed that the relationship between the NC and glycemia varies according to the stage of sexual maturation. As this maturation factor is rarely considered in the glycemic alteration screening, the use of the NC in this age group is not safe and requires further investigations.

关键词： Adolescents Glycemia Neck Circumference Sexual Maturation Puberty

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Effects of Microselective Neurotomy on Focal Spasticity and Description of the Surgical Technique

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Open Journal of Modern Neurosurgery 2021年第3期11卷 144-156页

作者： Sergio A. Sacchettoni Joyce Bolaños Napoleon Torres Juan P. Abud Penelope Mantilla Luis Salazar maria-fernanda Vargas Julio Rodriguez-Wulff Randy Balladares Ricardo Gomez Service of Neurosurgery Hospital Vargas de Caracas Caracas Venezuela Service of Neurosurgery Centro Medico Docente La Trinidad Caracas Venezuela Neurological Unit “NeuroQX” Centro Médico Integra Caracas Venezuela Université Grenoble Alpes Grenoble France

We studied the microselective neurotomy (MSN) and its advantages to alleviate disabling spasticity. The best indication for MSN is focal spasticity. We performed 298 MSN, 87 nerves on the upper limb, 211 nerves on the lower limb) in 68 patients. The initial causes were: stroke, cranial trauma, postoperative complications and multiple sclerosis. MSN procedures were performed on the median nerve (n = 40) for wrist and finger flexion;musculo-cutaneus nerve (n = 38) for elbow flexion;ulnaris nerve (n = 9) for cubital deviation of the hand;gastrocnemius nerve (n = 98) and soleus nerve (n = 49) for equinus foot, tibial posterior nerve (n = 45) for varus foot, and fascicles (or bundles) of the flexor digitorum for “claw” toes (n = 19). The main preoperative test to identify the responsible nerve was a neuromuscular block with local anesthesia (lidocaine or bupivacaine) injected into the site of the nerve connecting the spastic muscle. During surgery, the identified nerve was exposed and its epineurium opened. Nerve bundles were teased apart into individual rootlets and a number of rootlets cut were previously planned, according to the spasticity. Follow-up was performed for up to 10 years, with a mean period of 29 months. Results demonstrated a reduction of limb spasticity of 2 to 3 points: modified Ashworth scale (MAS). Pain and clonus were also diminished in the affected limb. In some cases, voluntary movement was once again possible. MSN is a useful alternative in those cases of focal spasticity where physiotherapy and nerve block with botulinum toxin or phenol no longer produce satisfactory results.

关键词： Focal Spasticity Microselective Neurotomy Neurosurgery of Spasticity

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Factors Associated with the Infection by <i>Plasmodium vivax</i>in Children and Adolescents from the Brazilian Amazon Basin

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Advances in Infectious Diseases 2020年第2期10卷 140-147页

作者： Rosa maria Dias Larissa Rodrigues da Costa Agostilina Renata dos Santos da Cruz Ramos Andrea Graças Ferreira Frazã o Clá udia Daniele Tavares Dutra fernanda maria Lima Moura Isameriliam Rosaulem Pereira da Silva José Luiz Fernandes Vieira Faculty of Nutrition Institute of Health Sciences Para Federal University Belem Brazil Pharmacy Faculty Campus Universitario do Guama Para Federal University Belem Brazil

Objective: To investigate the factors associated with malaria vivax infection in children and adolescents from an endemic area in the Eastern portion of the Brazilian Amazon basin. Methods: A case-control study was carried out in the municipality of Anajas, Brazil, from January 2016 to September 2017. Questionnaires were applied to the relative or the legal tutors of children and adolescents with questions regarding social and economic status, educational degree, knowledge about malaria, and the use of protective measures against mosquito bites. Anthropometric measures were performed in children and adolescents. Results: A total of 58 cases and 61 controls were included in the study. Most of the participants were male, eutrophics, and their relatives presented low educational degrees as well as low social and economic status. The knowledge of malaria was limited, but the percentage of individuals using long-lasting insecticidal nets was high. The presence of peri-domiciliary breeding sites for Anopheles vector was the most critical factor for malaria. Conclusion: The occurrence of malaria was associated with low social and economic status, a low educational degree, the unfamiliarity with malaria and the presence of breeding sites for Anopheles mosquito surrounding individual domiciles.

关键词： Infectious Diseases Malaria Epidemiology Risk Factor

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Revealing the kinetics of Leishmania chagasi infection in the male genital system of hamsters

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Infectious Diseases of Poverty 2016年第1期5卷 261-265页

作者： Amanda P.N.Quintal Bruna C.Borges Paula C.Brígido Rebecca T.Silva Ana F.Notário Marlus A.Santos maria A.de Souza fernanda G.O.Nascimento Antônio V.Mundim Guilherme M.J.Costa André B.Vasconcelos Claudio V.da Silva Instituto de Ciências Biomédicas Universidade Federal de UberlândiaUberlândiaMinas GeraisBrasil Hospital Veterinário Universidade Federal de UberlândiaUberlândiaMinas GeraisBrasil Departamento de Ciências Biomédicas Universidade Federal de Minas GeraisBelo HorizonteBrasil Universidade de Uberaba UberabaMinas GeraisBrasil Departamento de Imunologia–Instituto de Ciências Biomédicas Universidade Federal de UberlândiaCampus UmuaramaUberlândiaMGBrasil

Background:Leishmaniasis causes alterations and lesions in the genital system,which leads to azoospermia and testicular atrophy in animals during the chronic phase of the *** aim of this study was to reveal the kinetics of Leishmania chagasi infection in the genital system of male golden hamsters(Mesocricetus auratus).Methods:Animals were intraperitoneally inoculated with amastigotes from *** different time points animals were euthanized and genital organs processed for histo-pathological,qPCR,cytokines and testosterone detection ***:Our results showed a high parasite load in testis,followed by an increase of pro-inflammatory cytokines IL1-β,TNF-αand IFN-γ,and ***,IL-4 expression was upregulated and basal parasite persistence in testis was observed using the experimental ***:Extracellular amastigotes migrated to the epididymis posing as a potential major factor of parasite persistence and venereal transmission of *** infection in hamsters.

关键词： Leishmania chagasi Extracellular amastigotes Testis Epididymis Immune response Testosterone

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Serum proinflammatory cytokines and nutritional status in pediatric chronic liver disease

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世界胃肠病学杂志：英文版 2015年第29期21卷 8927-8934页

作者： Daniele Santetti maria Inês de Albuquerque Wilasco Cristina Toscani Leal Dornelles Isabel Cristina Ribas Werlang fernanda Urruth Fontella Carlos Oscar Kieling Jorge Luiz dos Santos Sandra maria Goncalves Vieira Helena Ayako Sueno Goldani Post-Graduate Program of Child and Adolescent Health Faculdade de MedicinaUniversidade Federal do Rio Grande do SulPorto Alegre Nutrition Service Hospital de Clínicas de Porto AlegreUniversidade Federal do Rio Grande do SulPorto Alegre Laboratory of Translational Pediatrics-NESCA Hospital de Clínicas de Porto AlegreUniversidade Federal do Rio Grande do SulPorto Alegre Pediatric Hepatology Unit Hospital de Clínicas de Porto AlegreUniversidade Federal do Rio Grande do SulPorto Alegre Pediatric Gastroenterology Unit Hospital de Clínicas de Porto Alegre and Post-Graduate Program of Child and Adolescent HealthFaculdade de MedicinaUniversidade Federal do Rio Grande do SulPorto Alegre

AIM: To evaluate the nutritional status and its association with proinflammatory cytokines in children with chronic liver ***: We performed a cross-sectional study with 43 children and adolescents, aged 0 to 17 years, diagnosed with chronic liver disease. All patients regularly attended the Pediatric Hepatology Unit and were under nutritional follow up. The exclusion criteria were fever from any etiology at the time of enrollment, inborn errors of the metabolism and any chronic illness. The severity of liver disease was assessed by Child-Pugh, Model for End-stage Liver Disease(MELD) and Pediatric End Stage Liver Disease(PELD) scores. Anthropometric parameters were height/age, body mass index/age and triceps skinfold/age according to World Health Organization standards. The cutoff points for nutritional status were risk of malnutrition(Z-score <-1.00) and malnutrition(Z-score <-2.00). Interleukin-1β(IL-1β), IL-6 and tumor necrosis factor-α levels were assessed by commercial ELISA kits. For multivariate analysis, linear regression was applied to assess the association between cytokine levels, disease severity and nutritional status. RESULTS: The median(25th-75 th centile) age of the study population was 60(17-116)-mo-old, and 53.5% were female. Biliary atresia was the main cause of chronic liver disease(72%). With respect to Child-Pugh score, cirrhotic patients were distributed as follows: 57.1% Child-Pugh A, a mild presentation of the disease, 34.3% Child-Pugh B, a moderate stage of cirrhosis and 8.6% Child-Pugh C, were considered severe cases. PELD and MELD scores were only above the cutoff point in 5 cases. IL-6 values were increased in patients at nutritional risk(34.9%) compared with those who were well-nourished [7.12(0.58-34.23) pg/m L vs 1.63(0.53-3.43) pg/m L; P = 0.02], correlating inversely with triceps skinfold-for-age z-score(rs =-0.61; P < 0.001). IL-6 levels were associated with liver disease severity assessed by Child-Pugh score(P = 0.001).

关键词： Cytokines Interleukin-6 Malnutrition Cirrhosis Child

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