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Altered blood-brain barrier permeability in rats with prehepatic portal hypertension turns to normal when portal pressure is lowered

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World Journal of Gastroenterology 2006年第9期12卷 1367-1372页

作者： Francisco Eizayaga Camila Scorticati Juan P Prestifilippo Salvador Romay maria A fernandez José L Castro Abraham Lemberg Juan C Perazzo Laboratory of Portal Hypertension Department of Pathophysiology School of Pharmacy and BiochemistryUniversity of Buenos AiresBuenos AiresArgentina Catedra de Farmacología School of Pharmacy and Biochemistry University of Buenos Aires Buenos Aires Argentina Laboratory of Portal Hypertension Department of Pathophysiology School of Pharmacy and Biochemistry University of Buenos Aires Buenos AiresArgentina

AIM： To study the blood-brain barrier integrity in prehepatic portal hypertensive rats induced by partial portal vein ligation, at 14 and 40 dafer ligation when portal pressure is spontaneously normalized. METHODS： Adult male Wistar rats were divided into four groups： Group Ⅰ： Sham14d, sham operated; Group Ⅱ： PHil, portal vein stenosis, （both groups were used 14 days after surgery）; Group Ⅲ： Sham4od, Sham operated and Group Ⅳ： PH4od Portal vein stenosis （Groups Ⅱ and Ⅳ used 40 d afer surgery）. Plasma ammonia, plasma and cerebrospinal fluid protein and liver enzymes concentrations were determined. Trypan and Evans blue dyes, systemically injected, were investigated in hippocampus to study blood-brain barrier integrity. Portal pressure was periodically recorded. RESULTS： Forty days afer stricture, portal pressure was normalized, plasma ammonia was moderately high, and both dyes were absent in central nervous system parenchyma. All other parameters were reestablished. When portal pressure was normalized and ammonia level was lowered, but not normal, the altered integrity of blood-brain barrier becomes reestablished. CONCLUSION： The impairment of blood-brain barrier and subsequent normalization could be a mechanism involved in hepatic encephalopathy reversibility. Hemodynamic changes and ammonia could trigger blood-brain barrier alterations and its reestablishment.

关键词： Blood-brain barrier Rats Prehepatic portal hypertension

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A role for the Tgf-β/Bmp co-receptor Endoglin in the molecular oscillator that regulates the hair follicle cycle

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Journal of Molecular Cell Biology 2019年第1期11卷 39-52页

作者： maria I.Calvo-Sanchez Sandra fernandez-Martos Elisa Carrasco Gema Moreno-Bueno Carmelo Bernabeu Miguel Quintanilla Jesus Espada Instituto de Investigaciones Biomedicas'Alberto Sols' Consejo Superior de Investigaciones Cientificas(CSIC)-Departamento de BioqufmicaUniversidad Autonoma de Madrid(UAM)Madrid 28029Spain Instituto de Investigaciones Biosanitarias Facultad de Ciencias ExperimentalesUniversidad Francisco de Vitoria(UFV)Pozuelo de Alarcon 28223Spain Centro de Investigacion Biomedica en Red de Cancer(CIBERONC) Spain Centro de Investigaciones Biologicas Consejo Superior de Investigaciones Cientificas(CSIC)and Centro de Investigacion Biomedica en Red de Enfermedades Raras(CIBERER)Madrid 28040Spain Centro Integrativo de Biologfa y Quimica Aplicada Universidad Bernardo O'HigginsSantiago 8370854Chile Instituto Ramon y Cajal de Investigacion Sanitaria(IRYCIS) Hospital Universitario Ramon y CajalMadridSpain

The hair follicle is a biological oscillator that alternates growth,regression,and rest phases driven by the sequential activation of the proliferation differentiation programs of resident stem cell *** activation of hair follicle stem cell niches and subsequent entry into the growing phase is mainly regulated by Wnt/β-catenin signalling,while regression and resting phases are mainly regulated by Tgf-β/Bmp/Smad activity.A major question still unresolved is the nature of the molecular switch that dictates the coordinated transition between both signalling *** we have focused on the role of Endoglin(Eng),a key co-receptor for members of the Tgf-β/Bmp family of growth *** an Eng haploinsufficient mouse model,we report that Eng is required to maintain a correct follicle cycling pattern and for an adequate stimulation of hair follicle stem cell *** further report thatβ-catenin binds to the Eng promoter depending on Bmp ***,we show thatβ-catenin interacts with Smad4 in a Bmp/Eng-dependent context and both proteins act synergistically to activate Eng promoter *** observations point to the existence of a growth/rest switching mechanism in the hair follicle that is based on an Eng-dependent feedback cross-talk between Wnt/β-catenin and Bmp/Smad signals.

关键词： Endoglin hair follicle skin stem cells Wnt/β-catenin Tgf-β/Bmp/Smad

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C-reactive protein is differentially modulated by co-existing infections,vitamin deficiencies and maternal factors in pregnant and lactating indigenous Panamanian women

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Infectious diseases of Poverty 2017年第1期6卷 819-832页

作者： Doris Gonzalez-fernandez Emerita del Carmen Pons Delfina Rueda Odalis teresa Sinisterra Enrique Murillo Marilyn E.Scott Kristine G.Koski School of dietetics and Human Nutrition Macdonald Campus of McGill University21111 Lakeshore RoadSte-Anne-de-BellevueQC H9X 3V9Canada Department of Nutritional Health Ministry of HealthAnconPanama CityPanama “Comarca Ngabe-Bugle”Health Region Ministry of HealthSan FelixChiriqui ProvincePanama “Panama Norte”Health Region Ministry of HealthLas Cumbres SquareTransithmian HighwayPanama CityPanama Department of Biochemistry University of PanamaSimon Bolivar Avenue(Transithmian Highway)Panama CityPanama Department of Biochemistry University of PanamaManuel Espinoza Batista and Jose De Fabrega AvenuesPanama CityPanama Institute of Parasitology and Centre for Host-Parasite Interactions Macdonald Campus of McGill University21111 Lakeshore RoadSte-Anne-de-BellevueQC H9X 3 V9Canada School of dietetics and Human Nutrition and Centre for Host-Parasite Interactions Macdonald CampusMcGill University21111 Lakeshore RoadSte-Anne-de-BellevueQC H9X 3 V9Canada

Background:The usefulness of C-reactive protein(CRP)as a non-specific marker of inflammation during pregnancy and lactation is unclear in impoverished populations where co-existing infections and vitamin deficiencies are ***:This cross-sectional study in Panama recruited 120 pregnant and 99 lactating Ngäbe-Buglewomen from 14 communities in rural *** history,indoor wood smoke exposure,fieldwork,BMI,vitamins A,B_(12),D,and folic acid,and inflammation markers(CRP,neutrophil/lymphocyte ratio(NLR),plateletcrit and cytokines)were *** regressions explored both associations of CRP with other inflammatory markers and associations of CRP and elevated CRP based on trimester-specific cut-offs with maternal factors,infections and vitamin ***:CRP was higher in pregnancy(51.4±4.7 nmol/L)than lactation(27.8±3.5 nmol/L)and was elevated above trimester specific cut-offs in 21%of pregnant and 30%of lactating *** deficiencies were common(vitamin A 29.6%;vitamin D 68.5%;vitamin B1268%;folic acid 25.5%)and over 50%of women had two or more concurrent deficiencies as well as multiple *** regression models highlighted differences in variables associated with CRP between pregnancy and *** pregnancy,CRP was positively associated with greater indoor wood smoke exposure,caries and hookworm and negatively associated with Ascaris and vaginal Lactobacillus and Bacteroides/Gardnerella *** with this,greater wood smoke exposure,caries as well as higher diplococcal infection score increased the odds of trimester-elevated CRP concentrations whereas longer gestational age lowered the likelihood of a trimester-elevated *** lactation,folic acid deficiency was associated with higher CRP whereas parity,number of eosinophils and Mobiluncus score were associated with lower ***,a higher BMI and Trichomonas vaginalis score increased the likelihood of an elevated CRP whereas higher parity and numbe

关键词： CRP Intestinal parasites Caries Vaginal infections Folic acid deficiency Wood smoke Neutrophil/lymphocyte ratio Plateletcrit Pregnancy Lactation

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dietary inclusion of a partially defatted black soldier fly(Hermetia illucens)larva meal in low fishmeal-based diets for rainbow trout(Oncorhynchus mykiss)

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Journal of Animal Science and Biotechnology 2021年第4期12卷 1399-1413页

作者： Christian Caimi Ilaria Biasato Giulia Chemello Sara Bellezza Oddon Carola Lussiana Vanda maria Malfatto maria teresa Capucchio Elena Colombino Achille Schiavone Francesco Gai Angela Trocino Alberto Brugiapaglia Manuela Renna Laura Gasco Department of Agricultural Forest and Food SciencesUniversity of TurinLargo P.Braccini 210095 GrugliascoTOItaly Department of Veterinary Sciences University of TurinLargo P.Braccini 210095 GrugliascoTOItaly Institute of Science of Food Production National Research CouncilLargo P.Braccini 210095 GrugliascoTOItaly Department of Comparative Biomedicine and Food Science University of PaduaViale dell’Università1635020 LegnaroPDItaly

Background:Recent investigations highlighted that Hermetia illucens(BSF)larva meal can be a valuable alternative protein source for aquafeed *** this study,in substitution of fishmeal,we used increasing inclusion levels of a partially defatted BSF larva meal in low fishmeal-based diets for rainbow trout(Oncorhynchus mykiss Walbaum)and we evaluated the related implications in terms of growth performance,physical characteristics,proximate and fatty acid(FA)compositions of the fillets,gut and liver histology,and diet *** a 131-day trial,576 fish(100.1±9.29 g)were randomly allotted to 24 tanks(24 fish/tank,4 replicates/treatment).Six experimental diets were produced to have partial replacement of fishmeal(0,10%,20%,30%,40%and 50%)by increasing levels of BSF meal(0%[BSF0],3%[BSF3],6%[BSF6],9%[BSF9],12%[BSF12]and 15%[BSF15]on as fed basis,respectively).Results:No differences were observed among the treatments for all the considered growth performance *** viscero and hepato-somatic indexes showed significant differences among the treatments,with the highest values observed in the BSF15 *** differences were recorded in terms of fillet’s physical characteristics,dry matter(DM),crude protein(CP)and ether extract(EE)*** saturated and monounsaturated FA increased,while polyunsaturated FA(particularly n-3 FA)decreased while increasing the HI meal inclusion in the *** of liver and gut was not affected,whereas,in posterior gut,villi were higher in BSF6 and BSF9 compared to BSF3 *** apparent digestibility of DM,CP,EE and gross energy did not vary among the ***:These results suggest that a partially defatted BSF meal could be included up to 15%in low fishmeal-based diets for rainbow trout with no adverse effects on growth performance,fillet’s physical characteristics,gut and liver health,and diet *** the contrary,the fillet FA composition worsened while increasing the level of BSF

关键词： Apparent digestibility coefficient Chemical and physical characteristics of fillets Fatty acid profile Fishmeal substitution Hermetia illucens meal Histopathology Performance

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A dissolution of Borderlines in Toni Morrison＇s God Help the Child

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Sociology Study 2017年第7期7卷 364-370页

作者： Grazyna maria teresa Branny Grazyna maria teresa Branny ul. Bulwarowa 31/3 31-751Krakow Poland

The paper focuses on Toni Morrison＇s latest novel God Help the Child （2015）. By presenting a skillful though somewhat perverse merger of binary oppositions at different levels （racial, social, moral, and psychological）, the writer makes borderlines of all sorts appear artificial and therefore invalidates them. Thus, childhood merges with adulthood through sexual traumas that live on; touch with no touch as the evil touch of a parent equals an abhorrence of touching the child Other; truth with a lie as it proves as destructive as lying in good faith; passing blackness with blue blackness as the former conceives the latter; and appearances with reality in the ironic title of the book, where it is both the mother and the child that in fact need God＇s help. Thus, as Toni Morrison demonstrates, a thoroughly surreptitious, because natural, process of dissolution of all barriers makes them appear to be arbitrary constructs responsible for the equally arbitrary notion of the Other. Taking an utterly holistic view of the nature of things, Morrison seems to suggest that borderlines are a consequence and a manifestation of a lack of balance, which therefore needs to be redressed through love, mutual understanding, and maturation.

关键词： Toni Morrison God Help the Child sexual traumas dissolution of borderlines the Other

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Demythologizing the South： Cormac McCarthy＇s Suttree in an Intertextual Perspective

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Journal of Literature and Art Studies 2017年第12期7卷 1543-1549页

作者： Grazyna maria teresa Branny Pedagogical University Cracow Poland

The article is structured around a premise of intertextuality, which is suggested not only by McCarthy＇s own more or less overt allusions to Faulkner＇s writing but also by the very name of his protagonist Suttree, which is evocative of the name of perhaps the best known Faulkner villain Thomas Sutpen. This supposition in turn leads to an argument that in his 1979 novel McCarthy does indeed reverse the life story of Thomas Sutpen by making Suttree descend down the very path that Sutpen ascended a century and a half before him, i.e., from the ranks of Southern aristocracy to the scum of the earth, and in defiance of the same ideology that Sutpen went to great lengths to embrace. Thus, an intertextual and comparative approach to McCarthy＇s novel not only in the context of Faulkner＇s Absalom, Absalom! but also his Light in August （cf. Joe Christmas） and The Sound and the Fury （cf. Quentin） as well as Ellen Glasgow＇s short story ＂Jordan＇s End＂ demonstrates that what Cormac McCarthy actually does in Suttree is to demythologize the South, complete with its aristocratic pretensions （＂doing pretty＂）, dubious morality （incest） and fear of miscegenation （obsession with time and the double）. Moreover, in doing so, he defamiliarizes it by reducing it to its Other （poor whites and African Americans）, whose authenticity, liveliness and charitability defy the affectation, lifelessness and decadence of the aristocratic South.

关键词： Cormac McCarthy William Faulkner the South intertextuality demythologization defamiliarization

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Management of single pulmonary metastases from colorectal cancer:State of the art

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World Journal of Gastrointestinal Oncology 2022年第4期14卷 820-832页

作者： Marco Chiappetta Lisa Salvatore maria teresa Congedo maria Bensi Viola De Luca Leonardo PetraccaCiavarella Floriana Camarda Jessica Evangelista Vincenzo Valentini Giampaolo Tortora StefanoMargaritora Filippo Lococo Department of Thoracic Surgery Fondazione Policlinico Universitario Agostino Gemelli IRCCS-UniversitàCattolica del Sacro CuoreRome 00168Italy Oncologia Medica Comprehensive Cancer Center Fondazione Policlinico Universitario Agostino Gemelli IRCCS-UniversitàCattolica del Sacro CuoreRome 00168Italy Department of Radiation Therapy Fondazione Policlinico Universitario Agostino Gemelli IRCCS-UniversitàCattolica del Sacro CuoreRome 00168Italy

Colorectal cancer(CRC)is one of the most common causes of death from *** seeding occurs in approximately 10%of patients surgically treated for primary CRC with radical intent:the lung is the most common site of metastases after the *** surgical treatment of liver metastases is widely accepted to affect long-term outcomes,more controversial and not standardized is the therapy for CRC patients developing lung *** suggests the potential curative role of pulmonary metastasectomy,especially in oligometastatic ***,the optimal strategy of care and the definition of prognostic factors after treatment still need to be *** review focused on the uncommon scenario of single pulmonary metastases from *** explored pertinent literature and provide an overview of the epidemiology,clinical characteristics and imaging of single pulmonary metastases from ***,we identified the best available evidence for overall *** particular,we analyzed the role and results of locoregional approaches(surgery,radiotherapy or ablative procedures)and their integration with systemic therapy.

关键词： Colorectal cancer Pulmonary metastases Oligometastases Chemotherapy Surgery Radiotherapy

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The Path of Undergraduate Intemational Relations Studies in Chile： Stumbling Under the Umbrella of Political Science

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International Relations and diplomacy 2017年第9期5卷 575-581页

作者： maria teresa Aya Smitmans Universidad Externado de Colombia Bogota Colombia Universidad Central Chile Santiago Chile

In Chile, the study of international relations is very limited both in the number of programs and in its scope. Furthermore, it is still a country where international relations are under the umbrella of political science and political science itself is heavily influenced by the study of the law. This paper looks at the development of international relations both as a tool for training diplomats and as a topic of study within political science in Chile. It does so within the framework of the social sciences. Using interviews as a complimentary tool of research, it concludes that, along with a marked influence of economics in the study of social sciences in Chile, the country＇s political and social history has shaped the advent of social sciences at the undergraduate level and has dwarfed the growth and contents of both political science and international studies, in spite of the country＇s successful embrace of globalization.

关键词： international studies political science Chile diplomatic training international relations (IR) programs

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Black soldier fly defatted meal as a dietary protein source for broiler chickens:Effects on growth performance,blood traits,gut morphology and histological features

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Journal of Animal Science and Biotechnology 2018年第4期9卷 891-900页

作者： Sihem Dabbou Francesco Gai Ilaria Biasato maria teresa Capucchio Elena Biasibetti Daniela Dezzutto Marco Meneguz Iveta Plachà Laura Gasco Achille Schiavone Department of Veterinary Science University of TurinLargo Paolo Braccini 210095 GrugliascoItaly Institute of Science of Food Production National Research CouncilLargo Paolo Braccini 210095 GrugliascoItaly Veterinary Medical Research Institute for Piemonte Liguria and Valle d’AostaVia Bologna 14810154 TurinItaly Department of Agricultural Forest and Food SciencesUniversity of TurinLargo Paolo Braccini 210095 GrugliascoItaly Institute of Animal Physiology Centre of BioscienceSlovak Academy of SciencesSoltesovej 4-6040 01 KosiceSlovak Republic Institute of Interdisciplinary Research on Sustainability University of TurinVia Accademia Albertina 1310100 TurinItaly

Background: The present study has evaluated the effects of different inclusion levels of a partially defatted black soldier fly(Hermetia illucens L.; HI) larva meal on the growth performance, blood parameters and gut morphology of broiler chickens. A total of 256 male broiler chickens(Ross 308) were reared from d 1 to d 35 and assigned to4 dietary treatments(8 replicates/treatment and 8 birds/replicate). HI larva meal was included at increasing levels(0, 5%, 10% and 15%; HI0, HI5, HI10 and HI15, respectively) in isonitrogenous and isoenergetic diets formulated for 3 feeding phases: starter(1–10 d), growing(10–24 d) and finisher(24–35 d). Two birds per pen were slaughtered at d 35 and morphometric investigations and histopathological alterations were ***: The live weight(LW) showed linear and quadratic responses to increasing HI larva meal(maximum for HI10 group). Average daily gain(ADG) showed a linear and quadratic responses to HI meal(maximum for HI10 group) during starter and growing periods. A linear decrease was observed for ADG during the finisher period. The daily feed intake(DFI) showed a linear and quadratic effect during the starter period(maximum for HI10 group).Linear and quadratic responses were observed for the feed conversion ratio(FCR) in the growing period and for the whole period of the experiment. The FCR showed a linear response in the finisher period(maximum for HI15).No significant effects were observed for the blood and serum parameters, except for the phosphorus concentration,which showed linear and quadratic responses as well as glutathione peroxidase(GPx) activity, the latter of which showed a linear response. The HI15 birds showed a lower villus height, a higher crypt depth and a lower villus height-to-crypt depth ratio than the other ***: Increasing levels of dietary HI meal inclusion in male broiler chickens may improve the LW and DFI during the starter period, but may also negatively affect the FCR and gut m

关键词： Broiler chickens Defatted insect meal Gut morphology Hermetia illucens Performance

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Mangiferin,a natural xanthone,accelerates gastrointestinal transit in mice involving cholinergic mechanism

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World Journal of Gastroenterology 2012年第25期18卷 3207-3214页

作者： Talita Cavalcante Morais Synara Cavalcante Lopes Karine maria Martins Bezerra Carvalho Bruno Rodrigues Arruda Francisco Thiago Correia de Souza maria teresa Salles Trevisan Vietla Satyanarayana Rao Flávia Almeida Santos Department of Physiology and Pharmacology Brazilian SemiArid Institute of BiomedicineFaculty of MedicineFederal University of CearáFortaleza 60430-270Brazil Post-Graduate Programme in Medical Sciences Faculty of MedicineFederal University of CearáFortaleza 60430-140Brazil Department of Organic and Inorganic Chemistry Federal University of CearáFortaleza 60451-970Brazil

AIM： To investigate the effects of mangiferin on gas- trointestinal transit （GIT） in normal and constipated mice, together with the possible ***： Intragastrically-administered charcoal mealwas used to measure GIT in overnight starved Swiss mice. In the first experiments, mangiferin （3 mg/kg, 10 mg/kg, 30 mg/kg, and 100 mg/kg, po） or tegaserod （1 mg/kg, ip） were administered 30 min before the char- coal meal to study their effects on normal transit. In the second series, mangiferin （30 mg/kg） was tested on delayed GIT induced by several different pharma- cological agonists （morphine, clonidine, capsaicin） or antagonists （ondansetron, verapamil, and atropine） whereas in the third series, mangiferin （30 mg/kg, 100 mg/kg and 300 mg/kg） or tegaserod （1 mg/kg） were tested on 6 h fecal pellets outputted by freely fed mice. The ratio of wet to dry weight was calculated and used as a marker of fecal water content. RESULTS： Mangiferin administered orally significantly （P 〈 0.05） accelerated GIT at 30 mg/kg and 100 mg/kg （89% and 93%, respectively）, similarly to 5-hydroxytrypta- mine4 （5-H%） agonist tegaserod （81%） when compared to vehicle-treated control （63%）. Co-administered man- giferin （30 mg/kg） totally reversed the inhibitory effect of opioid agonist morphine, 5-HT3-receptor antagonist ondansetron and transient receptor potential vanilloid-1 receptor agonist capsaicin on GIT, but only to a partial extent with the GIT-delay induced by ~2-adrenoceptor agonist clonidine, and calcium antagonist verapamil. However, co-administered atropine completely blocked the stimulant effect of mangiferin on GIT, suggesting the involvement of muscarinic acetylcholine receptor activation. Although mangiferin significantly enhanced the 6 h fecal output at higher doses （245.5±10.43 mg vs 161.9±10.82 mg and 227.1±20.11 mg vs 161.9±10.82 mg of vehicle-treated control, at 30 and 100 mg/ kg, P 〈 0.05, respectively）, the effect of tegaserod was more potent （297.4±7.42 mg vs 161.9±1

关键词： Mangiferin Glucosylxanthone Gastrointes-tinal transit Prokinetic action Cholinergic mechanism

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