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Chemical composition, antiparasitic and cytotoxic activities of aqueous extracts of Ziziphus joazeiro Mart

Asian Pacific Journal of Tropical Biomedicine 2019年第5期9卷 222-226页

作者： Jacqueline Cosmo ANDrade Ana Raquel pereira da Silva Ant?nia Thassya Lucas dos Santos maria Audilene Freitas Yedda maria Lobo Soares de Matos maria Flaviana Bezerra Morais Braga Camila Fonseca Bezerra maria isabeli pereira gon?alo maria Celeste Vega Gomez Míriam Rolóm Cathia Coronel Paulo Riceli Vasconcelos Ribeiro Edy Sousa de Brito Henrique Douglas Melo Coutinho Microbiology and Molecular Biology Laboratory Biological Chemistry Department Regional University of Cariri-URCA Bioassays Laboratory-LABIO Federal University of Cariri-UFCA Brejo Santo University campus (CE) Center for the Development of Scientific Research Moisés Bertoni Foundation/Díaz Gill Laboratories Natural Products Chemistry Multiuser Laboratory LMQPN Embrapa Tropical Agroindustry

Objective: To compare the in vitro antiparasitic activity of aqueous extracts from Ziziphus joazeiro leaves and stem bark against Trypanosoma cruzi, Leishmania braziliensis, and Leishmania infantum, as well as to evaluate its cytotoxicity in mammalian cells, in addition to identifying the chemical composition of the extracts. Methods: Ziziphus joazeiro leaf and stem bark aqueous extracts were prepared by cold extraction maceration and subjected to ultra-efficient liquid chromatography coupled to a quadrupole/time of flight system. The susceptibility assays used Trypanosoma cruzi CLB5 strains and promastigote forms of Leishmania braziliensis and Leishmania infantum for antiparasitic activity of the extracts. Moreover, mammalian fibroblasts NCTC clone 929 were used for cytotoxicity analysis. Results: Terpenoid compounds, flavonoids and phenolic acid were identified in extracts. The stem bark aqueous extracts presented more significant results in terms of antiparasitic activity compared with the leaf aqueous extracts, especially against Leishmania braziliensis and Leishmania infantum promastigote forms with an IC50 < 500 μg/mL. The cytotoxicity evaluation showed moderate toxicity of the stem bark aqueous extracts, which is relevant information for the rational use of this plant part since it is widely used by the population. Conclusions: These preliminary results may contribute to the formulation of new therapeutic agents against this group of neglected diseases, so further investigations are required to delineate the mechanisms of action mainly of the aqueous extract of stem bark of Ziziphus joazeiro.

关键词： Antiepimastigote Antipromastigote UPLC-MS-ESI-QTOF

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Challenges in Implementing DVI Best Practice Guidelines Due to the Brumadinho Disaster

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Forensic Medicine and Anatomy Research 2024年第1期12卷 1-12页

作者： Reinaldo Moreira Silva Bruno Diniz Sander Morais Carlos Danilo Ruas Silva Elza Cristina Lopes de Oliveira Fernando pereira Macedo Giovanni Vitral Pinto Guadalupe Dell’Arret Coutinho Higgor gonçalves Dornelas João Luiz Silva Moreira Laila Evangelista de Almeida Fernandes Marcia Lobão Iannini Patrícia Siqueira Valéria Rosalina Dias e Santos Sordaini maria Caligiorne Instituto de Criminalística Polícia Civil de Minas Gerais Belo Horizonte Brazil

The broke of a tailings dam from Vale S.A. destroyed a gigantic area killing 270 persons in Brumadinho/Brazil. Organizing activities after a mass disaster is a complex process that requires the involvement of many people and resources in the laboratory. To DNA identification of the victims, a daunting work had to be made by DNA laboratory staff in a collaborative effort with many partners. Efforts to implement good practice guidelines in Disaster Victim Identification (DVI) have revealed several important aspects that need to change in the forensic DNA laboratory. This article highlights the challenges of implementing DVI best practice guidelines in resource-poor settings, but with professionals from different sectors engaged in the same goal of briefly identifying victims and helping families and society.

关键词： Disaster Victim Identification DNA Brumadinho

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Conflicts in passive building performance:Retrofit and regulation of informal neighbourhoods

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Frontiers of Architectural Research 2021年第3期10卷 625-638页

作者： gonçalo Araújo Inês pereira António Leitão Manuel Correia Guedes INESC-ID/Instituto Superior Tećcnico University of LisbonLisbonPortugal Instituto Superior Tećcnico University of LisbonLisbonPortugal

Urbanization growth in developing countries raises concerns regarding these countries’ability to consider slums,underdeveloped communities,and neighbourhoods in economic,health,and climatic *** research proposes a methodology that integrates algorithmic design and analysis strategies to define,study,and measure key parameters that affect the rehabilitation of these *** scenarios and design dimensions are analysed to establish design and comfort thresholds,and alternatives are simulated and tested to identify possible *** methodology includes an optimisation step integrated in the workflow that maximizes thermal comfort,minimizes costs,and ensures fairness in the rehabilitation of large sets of *** step identifies improvements in thermal comfort for different construction scenarios from which a two-staged rehabilitation plan is *** first stage comprises a sensitivity analysis to identify building materials regarding their improvement and cost of application,and the second defines the most suitable construction scenarios considering the results from the optimisation process for each ***,we research and document guidelines regarding the parameters tested for building design,revealing the existing conflicts between performance objectives,and the architect’s role in their prioritization.

关键词： Urban regeneration Informal housing Algorithmic design Optimisation Simulation

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Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines

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World Journal of Gastroenterology 2012年第31期18卷 4162-4168页

作者： Raimundo Fernandes de Araújo Júnior Luiz Alberto Lira Soares Cínthia Raquel da Costa Porto Ranniere Gurgel Furtado de Aquino Hugo gon alo Guedes Pedro Ros Petrovick Tatiane pereira de Souza Aurigena Antunes de Araújo Gerlane Coelho Bernardo Guerra Department of Morphology/Postgraduate Program in Morphological Sciences Federal University of Rio Grande do NorteNatal 59072-970Rio Grande do NorteBrazil Postgraduate Program in Pharmaceutical Sciences Federal University of PernambucoRecife 50670-901PernambucoBrazil Postgraduate Program in Pharmaceutical Sciences Federal University of Rio Grande do NorteNatal 59012-570Rio Grande do NorteBrazil Department of Production and control of drugs Federal University of Rio Grande do SulPorto Alegre 90610-000Rio Grande do SulBrazil Department of Food and Drug Administration Federal University of AmazonasManaus 69077-000AmazonasBrazil Department of Biophysics and Pharmacology Federal University of Rio Grande do NorteNatal 59072-970Rio Grande do NorteBrazil

AIM:To investigate the cytotoxic effects of spray-dried extracts of Phyllanthus niruri in combination with cisplatin on two cancer cell ***:Colorectal carcinoma(HT29) and human hepatocellular carcinoma(HepG2) cells were treated with spray-dried extracts of Phyllanthus niruri(SDEPN) either alone or in combination with cisplatin at different concentrations(0.5 mg/mL and 1 mg/mL) for 4 h and 24 *** verify and quantify cancer cells treated with these products as well as identify the cell cycle stage and cell viability,we stained the cells with propidium iodide and assessed them by flow *** percentage of cells in different cell cycle phases was quantified and data were expressed as *** differences between groups were determined using analysis of variance and Bonferroni's test,as indicated.A value of P 0.05) and HepG2(5.07 ± 0.3 vs 15.9 ± 1.04,P 0.05) and HepG2(14.56 ± 1.6 vs 35.67 ± 3.94,P < 0.001) cells when compared to control cells for 24 *** cell lines were killed by cisplatin in a dose-dependent manner compared to control cells(HepG2 cells for 4 h:10.78 ± 1.58 vs 53.89 ± 1.53,P < 0.001;24 h:8.9 ± 1.43 vs 62.78 ± 1.87,P < 0.001 and HT29 cells for 4 h:9.52 ± 0.913 vs 49.86 ± 2.89,P < 0.001;24 h:11.78 ± 1.05 vs 53.34 ± 2.65,P < 0.001).In HT29 cells,pretreatment with SDEPN and subsequent treatment with cis-platin resulted in a greater number of cells being killed(12.78 ± 1.01 vs 93.76 ± 1.6,P < 0.001).HepG2 cells showed significant cell killing with treatment with SDEPN when combined with cisplatin(12.87 ± 2.78 vs 78.8 ± 3.02,P < 0.001).CONCLUSION:SDEPN is selectively toxic against two cancer cell ***,SDEPN in combinati

关键词：肿瘤细胞株提取物生长抑制作用顺铂珍珠 HepG2细胞细胞毒作用喷雾干燥

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The Storm Tracks Response to Changes in Atmospheric Greenhouse Gas Concentration at the South of Brazil and Southwest Atlantic Ocean

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Atmospheric and Climate Sciences 2019年第4期9卷 545-557页

作者： Rose Ane pereira De Freitas Fernanda Casagrande Douglas Da Silva Lindemann maria Angélica gonçalves Cardoso Jeferson Prietsch Machado Forecasting and Meteorological Research Center Federal University of Pelotas(UFPEL)PelotasBrazil Earth System Science Centre(CCST-INPE) National Institute for Space Research(INPE)Sao Jose dos CamposBrazil Federal University of Santa maria(UFSM) Santa MariaBrazil Oceanography Institute Federal University of Rio Grande(FURG)Rio GrandeBrazil

Here we investigate the ocean-atmosphere coupling and the contribution of the Sea Surface Temperature (SST) variations in: 1) Brazil-Malvinas Confluence (BMC) region, 2) Southwest Atlantic Ocean and 3) Southern Brazil. Numerical simulations of the ECHAM5/MPI-OM coupled ocean-atmosphere model were used to analyze the changes in the seasonal trajectory of the extratropical cyclones, in terms of intensification of physical mechanisms and implications for future scenarios. The numerical experiment for the future scenario considered an atmospheric CO2 concentration of approximately 770 ppm, which represents an increment of more than 350 ppm over the current values recorded by the Mauna Loa reference station. For this scenario, the results indicated a Storm Tracks (ST) displacement of 5° latitude toward south and changes of the meridional transport of sensible heat, close to 50°S. The increase in SST induces ST intensification and consequently an increase in the occurrence of extratropical cyclones. Overall, in the BMC region, we found a change in the pattern of cyclogenetic activity occurrence, with less frequent, but more intense events. On the Southern Brazilian region, the results of this study indicate increases in rainfall during summer months, whereas, a decrease in frequency and an increase in intensity were found for wintertime. We suggest that these changes could impact the climate dynamic of the Brazilian South coast, with a magnitude yet unknown.

关键词： Storm Tracks Confluence Brazil-Malvinas Atlantic Ocean

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External Morphology and Ultrastructure of Tegumental Glands of Aegla platensis(Crustacea,Anomura,Aeglidae)Pleopods:Might They Play A Role in Egg Attachment?

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Research in Ecology 2019年第2期1卷 1-7页

作者： Tainãgonçalves Loureiro Mauricio pereira Almerão maria Cristina Faccioni-Heuser Georgina Bond-Buckup Paula Beatriz de Araujo Marine Lab Department of Botany and ZoologyStellenbosch UniversityStellenboschSouth Africa UNILASALLE CanoasBrazil Department of Morphological Sciences Federal University of Rio Grande do SulPorto AlegreBrazil Laboratory of Carcinology Department of ZoologyFederal University of Rio Grande do SulPorto AlegreBrazil

Egg incubation on the female abdomen is the parental care behavior observed in aeglids,in which eggs are kept adhered to maternal pleopods and maintained,cleaned and *** ***,egg attachment occurs with the aid of pleopodal setae,which are twisted around their axis in the distal region,forming the funiculus,and pleopodal glands,which are responsible for the production of the adhesive substance that seems to be involved in egg fixation to pleopodal *** glands are acini formed by secretory cells arranged concentrically around a central duct,giving them a rosette *** types of secretory cells were observed,those that produce electron-lucid vesicles and those having electron-dense *** kinds of vesicles are released in a duct whose opening pore is located on the pleopodal surface and constitute the adhesive substance that coats eggs and pleopodal setae,ensuring egg fixation to the female body and maternal care *** study investigates the internal and external morphology of Aegla platensis pleopods,to understand the egg attachment process and identify the structures involved in this *** microscopy techniques are used:scanning electron microscopy(SEM),transmission electron microscopy(TEM),and optical microscopy(OM).

关键词： Egg incubation Pleopodal setae Funiculus Electron microscopy Optical microscopy

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The Storm Tracks Response to Changes in Atmospheric Greenhouse Gas Concentration at the South of Brazil and Southwest Atlantic Ocean

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Atmospheric and Climate Sciences 2019年第4期

作者： Rose Ane pereira De Freitas Fernanda Casagrande Douglas Da Silva Lindemann maria Angélica gonçalves Cardoso Jeferson Prietsch Machado

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The Antiproliferative and Pro-apoptotic Role of 2-aminoethyl dihydrogen Phosphate in Triple-negative Breast Tumor Cells

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Journal of Pharmacy and Pharmacology 2021年第4期9卷 160-173页

作者： Antenor pereira Bonfim Neto Laertty Garcia de Sousa Cabral Thalles Anthony Duarte Oliveira JulioCarlstron Pacheco Monique gonçalves Alves Mercedes Reyes Hernández Rosa Andreia Nogueira Laiso Durvanei Augusto maria University of São Judas Sao PauloSP 01246-000Brazil Faculty of Medicine University of Sao Paulo(FMUSP)Sao PauloSP 01246-000Brazil Laboratory of Development and Innovation Butantan InstituteSao PauloSP 05503-900Brazil Faculty of Veterinary Medicine and Animal Science University of São Paulo(FMZUSP)São PauloSP 01246-000Brazil

Antineoplastic phospholipids are a new class of antitumor *** molecules interact with the plasma membrane,changing numerous pathways that induce cell death,with high selectivity for cancer cells.A representative of this class of antineoplastic agents is 2-aminoethyl dihydrogen phosphate(2-AEH2P).It is present in high intracellular concentrations in various tissues and organelles with antitumor,antiproliferative and pro-apoptotic ***,4T1 triple-negative tumor cells were treated in different concentrations in order to assess the cytotoxic potential and its effects on the modulation of cell death pathways in association with the chemotherapeutic drug Paclitaxel.2-AEH2P promoted cytotoxicity in tumor cells and significant morphological changes,however,it did not cause these effects in normal *** was positive regulation of proteins involved in the intrinsic pathway of cell death by apoptosis and regulation of the phases of cell cycle ***,structural and distribution changes in mitochondria,as well as decreased cell density and regression of the cytoskeleton were *** 2-AEH2P demonstrated a modulatory potential of apoptotic pathways inducing cell death,being a new compound with antitumor properties.

关键词： Breast cancer 2-aminoethyl dihydrogen phosphate apoptosis cell cycle

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Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni

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World Journal of Gastroenterology 2008年第38期14卷 5842-5850页

作者： Sheilla Andrade Oliveira Bruno Solano Freitas Souza Carla Adriana Guimares-Ferreira Elton Sá Barreto Siane Campos Souza Luiz Antonio Rodrigues Freitas Ricardo Ribeiro-dos-Santos Milena Botelho pereira Soares Centro de Pesquisas gonalo Moniz Fundao Oswaldo Cruz Rua Waldemar Falco 121 Brotas 40295-001 Salvador BA Brazil Centro de Pesquisas Aggeu Magalhes Fundao Oswaldo Cruz Recife Av Moraes Rego s/n Cidade Universitária Recife 50670-420 PE Brazil Centro de Pesquisas gonalo Moniz Fundao Oswaldo Cruz Rua Waldemar Falco 121 Brotas Salvador 40295-001 BA Brazil Centro de Pesquisas gonalo Moniz Fundao Oswaldo Cruz Rua Waldemar Falco 121 Brotas 40295-001 Salvador BA Brazil Hospital So Rafael Av So Rafael 2152 So Marcos Salvador 41256-900 BA Brazil

AIM: To investigate the potential of bone marrow mononuclear cells (BM-MCs) in the regeneration of hepatic lesions induced by Schistosoma mansoni (***) chronic infection. METHODS: Female mice chronically infected with *** were treated with BM-MCs obtained from male green fluorescent protein (GFP) transgenic mice by intravenous or intralobular injections. Control mice received injections of saline in similar conditions. Enzyme-linked immunosorbent assay (ELISA) assay fortransforming growth factor-beta (TGF-β), polymerase chain reaction (PCR) for GFP DNA, immunofluorescence and morphometric studies were performed. RESULTS: Transplanted GFP+ cells migrated to granuloma areas and reduced the percentage of liver fibrosis. The presence of donor-derived cells was confirmed by Fluorescence in situ hybridization (FISH) analysis for detection of cells bearing Y chromosome and by PCR analysis for detection of GFP DNA. The levels of TGF-β, a cytokine associated with fibrosis deposition, in liver fragments of mice submitted to therapy were reduced. The number of oval cells in liver sections of ***-infected mice increased 3-4 fold after transplantation. A partial recovery in albumin expression, which is decreased upon infection with ***, was found in livers of infected mice after cellular therapy. CONCLUSION: In conclusion, transplanted BMCs migrate to and reduce the damage of chronic fibrotic liver lesions caused by ***.

关键词：肝损害肝纤维化骨髓细胞慢性感染动物模型治疗

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Fungal diversity notes 1277-1386:taxonomic and phylogenetic contributions to fungal taxa

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Fungal Diversity 2020年第5期104卷 1-266页

作者： Hai-Sheng Yuan Xu Lu Yu-Cheng Dai Kevin D.Hyde Yu-He Kan Ivana Kušan Shuang-Hui He Ning-Guo Liu V.Venkateswara Sarma Chang-Lin Zhao Bao-Kai Cui Nousheen Yousaf Guangyu Sun Shu-Yan Liu Fang Wu Chuan-Gen Lin Monika C.Dayarathne Tatiana Baptista Gibertoni Lucas B.Conceição Roberto Garibay-Orijel Margarita Villegas-Ríos Rodolfo Salas-Lizana Tie-Zheng Wei Jun-Zhi Qiu Ze-Fen Yu Rungtiwa Phookamsak Ming Zeng Soumitra Paloi Dan-Feng Bao Pranami DAbeywickrama De-Ping Wei Jing Yang Ishara S.Manawasinghe Dulanjalee Harishchandra Rashika S.Brahmanage Nimali Ide Silva Danushka S.Tennakoon Anuruddha Karunarathna Yusufjon Gafforov Dhandevi Pem Sheng-Nan Zhang AndréL.C.Mde Azevedo Santiago Jadson Diogo pereira Bezerra Bálint Dima Krishnendu Acharya Julieta Alvarez-Manjarrez Ali H.Bahkali Vinod K.Bhatt Tor Erik Brandrud Timur S.Bulgakov E.Camporesi Ting Cao Yu-Xi Chen Yuan-Yuan Chen Bandarupalli Devadatha Abdallah M.Elgorban Long-Fei Fan Xing Du Liu Gao Camila Melo gonçalves Luis F.P.Gusmão Naruemon Huanraluek Margita Jadan Ruvishika S.Jayawardena Abdul Nasir Khalid Ewald Langer Diogo X.Lima Nelson Correia de Lima-Júnior Carla Rejane Sousa de Lira Jian-Kui(Jack)Liu Shun Liu Saisamorn Lumyong Zong-Long Luo Neven Matočec M.Niranjan JoséRibamar Costa Oliveira-Filho Viktor Papp Eduardo Pérez-Pazos Alan J.L.Phillips Peng-Lei Qiu Yihua Ren Rafael F.Castañeda Ruiz Kamal C.Semwal Karl Soop Carlos A.Fde Souza Cristina maria Souza-Motta Li-Hua Sun Meng-Le Xie Yi-Jian Yao Qi Zhao Li-Wei Zhou CAS Key Laboratory of Forest Ecology and Management Institute of Applied EcologyChinese Academy of SciencesShenyang 110164People’s Republic of China University of the Chinese Academy of Sciences Beijing 100049People’s Republic of China School of Ecology and Nature Conservation Beijing Advanced Innovation Center for Tree Breeding By Molecular DesignBeijing Forestry UniversityBeijing 100083People’s Republic of China CAS Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanPeople’s Republic of China Centre of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Mushroom Research Foundation 128 M.3 Ban Pa Deng T.Pa PaeA.Mae TaengChiang Mai 50150Thailand East and Central Asia Regional Office World Agroforestry Centre(ICRAF)Kunming 650201YunnanChina Ruđer BoškovićInstitute Bijenička cesta 5410000 ZagrebCroatia Faculty of Agriculture Natural Resources and EnvironmentNaresuan UniversityPhitsanulok 65000Thailand Department of Biotechnology School of Life SciencesPondicherry UniversityR.V.NagarKalapetPondicherry 605014India College of Biodiversity Conservation Southwest Forestry UniversityKunming 650224People’s Republic of China Department of Botany Govt.College UniversityKatchery RoadLahorePakistan State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection Northwest A&F UniversityYanglingShaanxiPeople’s Republic of China Laboratory of Plant Pathology College of Plant ProtectionJilin Agricultural UniversityChangchun 130118Jilin ProvincePeople’s Republic of China Centro de Biociências Departamento de MicologiaUniversidade Federal de PernambucoCidade UniversitáriaAvenida da Engenharia s/nRecifePernambuco 50740-600Brazil Departamento de Ciências Biológicas Universidade Estadual de Feira de SantanaAv.Transnordestina s/nNovo HorizonteFeira de SantanaBA 44036-900Brazil L

This is the twelfth contribution to the Fungal Diversity Notes series on fungal taxonomy,based on materials collected from many countries which were examined and described using the methods of morphology,anatomy,and strain culture,combined with DNA sequence analyses.110 taxa are described and illustrated,including five new genera,92 new species,eight new combinations and other taxonomic contributions(one new sequenced species,one new host and three new records)which are accommodated in 40 families and 1 incertae sedis in *** new genera are Amyloceraceomyces,Catenuliconidia,Hansenopezia,Ionopezia and *** new species are Amyloceraceomyces angustisporus,Amylocorticium ellipsosporum,Arthrinium sorghi,Catenuliconidia uniseptata,Clavulina sphaeropedunculata,Colletotrichum parthenocissicola,Coniothyrium triseptatum,Cortinarius indorusseus,***,***,***,***,Diaporthe pimpinel-lae,Dictyosporella guizhouensis,Diplodia torilicola,Fuscoporia marquesiana,***,Hansenopezia decora,Helicoarcta-tus thailandicus,Hirsutella hongheensis,Humidicutis brunneovinacea,Lentaria gossypina,***,Lycoperdon lahorense,***,Magnopulchromyces scorpiophorus,Moelleriella gracilispora,Neodevriesia manglicola,Neodidymelliopsis salvia,***,Neoroussoella magnoliae,Neottiella gigaspora,Ophiosphaerella chiangraiensis,Phaeotremella yunnanensis,Podosphaera yulii,Rigidoporus juniperinus,Rhodofomitopsis pseudofeei,Russula benghalensis,Scleroramularia vermispora,Scytinopogon minisporus,Sporormurispora paulsenii,Thaxteriellopsis obliqus,Tomentella asiae-orientalis,***,***,***,***,***,***,***,***,***-nocystidiata,***,***,***,***,***,***,***,***,***,***,***,***,***,***,***,

关键词： 110 taxa Agaricomycetes Ascomycota Basidiomycota Mucoromycota New combinations New genera New species Phylogeny Taxonomy

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