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Araucari. angusti.oli. Aboveground Roots Presented Hi.h Arbuscular Mycorrhi.al Fungal Coloni.ati.n and Di.ersi.y i. the Brazi.i.n Atlanti. Forest

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Pedosphere 2016年第4期26卷 561-566页

作者： Mi.ene MOREi.A maria i. zucchi Jose E. GOMES Si. M. TSAi.Alessandro ALVES-PEREi.A Elke J. B. N. CARDOSO Sao Paulo State Agency for Agri.usi.ess Technology CP 28 Piracicaba 13400-970 Center for Nuclear Energy i. Agri.ulture University of Sao Paulo Piracieaba 13416-000 Geneti.s Department Luiz de Queiroz College of Agriculture University of Sao Paulo Piraeieaba 13418-900 Soi. Sci.nce Department Luiz de Queiroz College of Agriculture University of Sao Paulo Piracicaba 13418-900

Almost 30 di.ferent arbuscular mycorrhi.al fungi.AMF) speci.s, di.tri.uted i. di.ferent genera such as Glomus, Acaulospora,Scutellospora, Entrophospora, Ambi.pora, Kuklospora, Gi.aspora, and Archeospora, have been i.enti.i.d i. the root zone of Araucari. angusti.oli., known as Brazi. Pi.e. Duri.g our AMF survey i. thi. ecosystem, our attenti.n was called to the presence of many superfi.i.lly growi.g Araucari. roots. Our hypothesi. was that these roots were coloni.ed wi.h AMF because of the presence of AMF spores i. organi. materi.l aboveground. Samples of these superfi.i.l roots and the organi. substrate they were growi.g on were evaluated for thei. mycorrhi.al status. DNA was extracted from the AMF-coloni.ed superfi.i.l roots and submi.ted to polymerase chai. reacti.n(PCR) ampli.i.ati.n usi.g the NS31-AM1 pri.er pai., followed by cloni.g and sequenci.g. We found that the root coloni.ati.n percentages were between 31% and 52%, and the number of AMF spores i. the substrate ranged from 27 to 164 spores per 50 g dry substrate. The phylogeneti. analyses and tree constructi.n usi.g maxi.um parsi.ony(MP) and nei.hbor-joi.i.g(NJ)methods i.enti.i.d 13 di.ferent speci.s of the phylum Glomeromycota belongi.g to the genera Glomus, Funneli.ormi., Rhi.ophagus,Gi.aspora, Acaulospora, and Archaeospora, and fi.e i.olates were i.enti.i.d only at the genus level. To our knowledge, thi. i. the fi.st report on Araucari. angusti.oli. wi.h roots growi.g aboveground, produci.g runner roots that develop on dead tree trunks and organi. materi.l. The hi.her coloni.ati.n of the aboveground roots than those commonly found i. belowground Araucari. roots suggests that they may present acti.e metaboli. uptake of nutri.nts.

关键词： AMF spores fungal taxonomy mycorrhi.al status nutri.nt uptake organi. substrate root coloni.ati.n superfi.i.l roots

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Multi.aceted superoxi.e di.mutase 1 expressi.n i. amyotrophi. lateral sclerosi. pati.nts:a rare occurrence?

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Neural Regenerati.n Research 2025年第1期20卷 130-138页

作者： i.ari. Marti.elli.Jessi.a Mandri.li.Andrea Ghezzi.Eli.abetta zucchi Gi.li. Gi.nferrari.Ceci.i. Si.oni.i.Francesco Cavalli.ri.Franco Valzani. Department of Neurosci.nces Azienda Ospedaliero Universitaria di ModenaModenaItaly Cli.i.al and Experi.ental Medi.i.e Ph.D.Program University of Modena and Reggio EmiliaModenaItaly Department of Bi.medi.al Metabolic and Neural SciencesUniversity of Modena and Reggio EmiliaModenaItaly Neurology Uni. Neuromotor&Rehabilitation DepartmentAzienda USL-IRCCS di Reggio EmiliaReggio EmiliaItaly

Amyotrophi. lateral sclerosi.(ALS)i. a neuromuscular condi.i.n resulti.g from the progressi.e degenerati.n of motor neurons i. the cortex,brai.stem,and spi.al *** the typi.al cli.i.al phenotype of ALS i.volves both upper and lower motor neurons,human and ani.al studi.s over the years have hi.hli.hted the potenti.l spread to other motor and non-motor regi.ns,expandi.g the phenotype of *** superoxi.e di.mutase 1(SOD1)mutati.ns represent a mi.ori.y of ALS cases,the SOD1 gene remai.s a mi.estone i. ALS research as i. represents the fi.st geneti. target for personali.ed *** numerous si.gle case reports or case seri.s exhi.i.i.g extramotor symptoms i. pati.nts wi.h ALS mutati.ns i. SOD1(SOD1-ALS),no studi.s have comprehensi.ely explored the full spectrum of extramotor neurologi.al mani.estati.ns i. thi. *** thi. narrati.e revi.w,we analyze and di.cuss the avai.able li.erature on extrapyrami.al and non-motor features duri.g *** multi.aceted expressi.n of SOD1 could deepen our understandi.g of the pathogeni. mechani.ms,poi.ti.g towards a multi.i.ci.li.ary approach for affected pati.nts i. li.ht of new therapeuti. strategi.s for SOD1-ALS.

关键词： amyotrophi. lateral sclerosi.(ALS) autonomi. extramotor genotype-phenotype multi.ystem i.volvement Parki.son’s di.ease sensory SOD1 superoxi.e di.mutase 1 uri.ary vocal cord palsy

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The landscape of cogni.i.e i.pai.ment i. superoxi.e di.mutase 1-amyotrophi. lateral sclerosi.

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Neural Regenerati.n Research 2023年第7期18卷 1427-1433页

作者： i.ari. Marti.elli.Eli.abetta zucchi Ceci.i. Si.oni.i.Gi.li. Gi.nferrari.Gi.vanna Zamboni.Marcello Pi.ti.Jessi.a Mandri.li Cli.i.al and Experi.ental Medi.i.e PhD Program University of Modena and Reggio EmiliaModenaItaly Department of Neurosci.nces Azienda Ospedaliero-Universitaria di ModenaModenaItaly Department of Bi.medi.al Metabolic and Neural SciencesUniversity of Modena and Reggio EmiliaModenaItaly Department of Li.e Sci.nces University of Modena and Reggio EmiliaModenaItaly

Although mutati.ns i. the superoxi.e di.mutase 1 gene account for only a mi.ori.y of total amyotrophi. lateral sclerosi. cases,the di.covery of thi. gene has been cruci.l for amyotrophi. lateral sclerosi. *** the i.enti.i.ati.n of superoxi.e di.mutase 1 i. 1993,the fi.ld of amyotrophi. lateral sclerosi. geneti.s has consi.erably wi.ened,i.provi.g our understandi.g of the di.erse pathogeni. basi. of amyotrophi. lateral *** thi. revi.w,we focus on cogni.i.e i.pai.ment i. superoxi.e di.mutase 1-amyotrophi. lateral sclerosi. *** has mostly reported that cogni.i.n remai.s i.tact i. superoxi.e di.mutase 1-amyotrophi. lateral sclerosi. pati.nts,but recent reports hi.hli.ht frontal lobe functi.n frai.ty i. pati.nts carryi.g di.ferent superoxi.e di.mutase 1-amyotrophi. lateral sclerosi. *** thoroughly revi.wed all the vari.us mutati.ns reported i. the li.erature to contri.ute to a comprehensi.e database of superoxi.e di.mutase 1-amyotrophi. lateral sclerosi. genotype-phenotype *** a resource could ulti.ately i.prove our mechani.ti. understandi.g of amyotrophi. lateral sclerosi.,enabli.g a more robust assessment of how the amyotrophi. lateral sclerosi. phenotype responds to di.ferent vari.nts across genes,whi.h i. i.portant for the therapeuti. strategy targeti.g geneti. *** i. superoxi.e di.mutase 1-amyotrophi. lateral sclerosi. deserves further longi.udi.al research si.ce thi. peculi.r frai.ty i. pati.nts wi.h si.i.ar mutati.ns can be condi.i.ned by external factors,i.cludi.g envi.onment and other uni.enti.i.d agents i.cludi.g modi.i.r genes.

关键词： amyotrophi. lateral sclerosi. cogni.i.e i.pai.ment genotype-phenotype correlati.n superoxi.e di.mutase 1

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i.enti.i.ati.n of Soybean Genes i.volved i. Ci.cadi.n Clock Mechani.m and Photoperi.di. Control of Floweri.g Ti.e by i. Si.i.o Analyses

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Journal of i.tegrati.e Plant Bi.logy 2007年第11期49卷 1640-1653页

作者： VeraQueci.i.mariai.zucchi JoséBaldi. NatalA.Vello i.sti.utoAgron mi.o de Campi.as Centro de Pesquisa e Desenvolvimento de Recursos GenéticosCampinasSP 13075-630Brazil Departamento de Genéti.a ESALQUniversidade de Sāo PauloPiracicabaSP 13400-970Brazil

Glyci.e max i. a photoperi.di. short-day plant and the practi.al consequence of the response i. lati.ude and sowi.g peri.d li.i.ati.ns to commerci.l crops. Geneti. and physi.logi.al studi.s usi.g the model plants Arabi.opsi. thali.na and ri.e （Oryza sati.a） have uncovered several genes and geneti. pathways controlli.g the process, however i.formati.n about the correspondi.g pathways i. legumes i. scarce. Data mi.i.g predi.ti.n methodologi.s, i.cludi.g multi.le sequence ali.nment, phylogeneUc analysi., bi.i.formaUcs expressi.n and sequence moti. pattern i.enti.i.ati.n, were used to i.enti.y soybean genes i.volved i. day length percepti.n and photoperi.di. floweri.g i.ducti.n. We have i.vesti.ated approxi.ately 330 000 sequences from open-access databases and have i.enti.i.d all bona fi.e central osci.lator genes and ci.cadi.n photoreceptors from A. thali.na i. soybean sequence databases. We propose a worki.g model for the photoperi.di. control of floweri.g ti.e i. G. max, based on the i.enti.i.d key components. These results demonstrate the power of comparati.e genomi.s between model systems and crop speci.s to eluci.ate the several aspects of plant physi.logy and metaboli.m.

关键词： ci.cadi.n clock cryptochrome data mi.i.g floweri.g photoperi.d phytochrome.

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CONTRi.UTO ALLA CONOSCENZA DELLA FLORA BRESCi.NA Ⅰ.Flora vascolare della valle del fi.me Ogli. nell'Orceano

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Natural Di.aster Reducti.n i. Chi.a 1978年第1期 139-168页

作者： COSTANZA zucchi

i.TRODUZi.NEi. presente catalogo ri.uarda pri.ci.almente i. tratto plani.i.le allasi.i.tra del fi.me *** tratto è denomi.ato *** a di.edi.*** comune della bassa bresci.na confi.ante con la pro-vi.ci. di.*** maggi.r parte dell’Orceano è occupato dai.campi.olti.ati.che spesso si.spi.gono fi.o al fi.me,tultavi. alcune parti.sonoancora relati.amente i.tatle:la presente i.dagi.e si.è concentrata sulla fa-

关键词： Mi.ler 一弓 110 Flora vascolare della valle del fi.me Ogli. nell’Orceano CONTRi.UTO ALLA CONOSCENZA DELLA FLORA BRESCi.NA 李一 Ca

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Pelvi. muscle floor rehabi.i.ati.n as a therapeuti. opti.n i. li.elong premature ejaculati.n： long-term outcomes

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Asi.n Journal of Andrology 2018年第6期20卷 572-575页

作者： Antoni. Lui.i.Pastore Gi.vanni.Palleschi.Andrea Fuschi.Yazan Ai.Salhi.Alessandro zucchi Gi.rgi. Bozzi.i.Ester i.li.no Eli.abetta Costanti.i.Antoni. Carbone Uroi.gy Uni. Department of Medico-Surgical Sciences and Biotechnologies ICOT Faculty of Pharmacy and Medicine 'Sapienza' University of Rome Latina (LT) 04100 Italy Uroresearch Nonprofit Association for Research in Urology Latina 04100 Italy Department of Urology and Andrology University of Perugia Perugia 05156 Italy Department of Urology MATER DOMINI Humanitas Castellanza (VA) 21053 Italy

The ai. of the study was to evaluate the long-term outcomes of pelvi. floor muscle （PFM） rehabi.i.ati.n i. males wi.h li.elong premature ejaculati.n （PE）, usi.g i.travagi.al ejaculatory latency ti.e （i.LT） and the self-report Premature Ejaculati.n Di.gnosti. Tool （PEDT） as pri.ary outcomes. A total of 154 parti.i.ants were retrospecti.ely revi.wed i. thi. study, wi.h 122 completi.g the trai.i.g protocol. At baseli.e, all parti.i.ants had an i.LT 〈60 s and PEDT score 〉11. Parti.i.ants completed a 12-week program of PFM rehabi.i.ati.n, i.cludi.g physi.-ki.esi.therapy treatment, electrosti.ulati.n, and bi.feedback, wi.h three sessi.ns per week, wi.h 20 mi. for each component completed at each sessi.n. The effecti.eness of i.terventi.n was evaluated by compari.g the change i. the geometri. mean of i.LT and PEDT values, from baseli.e, at 3, 6, and 12 months duri.g the i.terventi.n, and at 24 and 36 months posti.terventi.n, usi.g a pai.ed sample 2-tai.ed t-test, i.cludi.g the associ.ted 95% confi.ence i.tervals. Of the 122 parti.i.ants who completed PFM rehabi.i.ati.n, 111 gai.ed control of thei. ejaculati.n reflex, wi.h a mean i.LT of 161.6 s and PEDT score of 2.3 at the 12-week endpoi.t of the i.terventi.n, representi.g an i.crease from baseli.e of 40.4 s and 17.0 scores, respecti.ely, for i.LT and PEDT （P 〈 0.0001）. Of the 95 parti.i.ants who completed the 36-month follow-up, 64% and 56% mai.tai.ed sati.factory ei.culati.n control at 24 and 36 months oosti.terventi.n, respecti.ely.

关键词： bi.feedback electrosti.ulati.n i.travagi.al ejaculatory latency ti.e pelvi. floor rehabi.i.ati.n premature ejaculati.n premature ejaculati.n di.gnosti. tool

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Long-term results of the surgi.al treatment of Peyroni.＇s di.ease wi.h Egydi.＇s techni.ue： a European multi.entre study

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Asi.n Journal of Andrology 2011年第6期13卷 842-845页

作者： Salvatore Sansalone Gi.li. Garaffa Rados Dji.ovi. Stefano Pecoraro Mauro Si.vani.Gui.o Barbagli.Alessandro zucchi Gi.seppe Vespasi.ni.Carla Loreto Department of Urology School of Medicine Tor Vergata University of Rome Rome 00133 italy St Peter＇s Andrology University College London Hospitals London WIG 6BJ UK Department of Urology School of Medicine Serbian Academy of Science and Arts University of Belgrade Belgrade 11000 Serbia Department of Nephro-Urology Malzoni Medical Center Avellino Avellino 83100 Italy Department of Urology General Hospital Biella 13900 Italy Center for Reconstructi.e Urethral Surgery Arezzo 52100 Italy Department of Urology and Andrology University of Perugia Perugia 06123 Italy Department of Bi.-Medi.al Sci.nces Anatomy Section University of Catania Catania 95100 Italy

The long-term outcomes of 157 pati.nts affected by Peyroni.＇s di.ease （PD） who underwent peni.e strai.hteni.g wi.h Egydi.＇s techni.ue between January 2004 and December 2008 are reported. Only pati.nts wi.h PD who were stable for at least 6-12 months pri.r to surgery were enrolled i. thi. study. Preoperati.e assessment i.cluded a dynami. echo colour Doppler ultrasound scan to evaluate the degree of peni.e deformi.y and the peak systoli. veloci.y i. the cavernosal arteri.s and an assessment of erecti.e functi.n wi.h the admi.i.trati.n of the i.ternati.nal i.dex of Erecti.e Functi.n 5 （i.EF-5） questi.nnai.e. Stretched peni.e length was recorded pre- and postoperati.ely. Surgi.al compli.ati.ns, cosmesi. and sexual functi.n, pati.nt sati.facti.n and postoperati.e erecti.e functi.n were assessed postoperati.ely at 3 months, i.year and 2 years, respecti.ely. After a medi.n follow-up peri.d of 20 months （range： 12-24 months）, we found that mi.d resi.ual curvature （12%） and glans hypoesthesi. （3%） were the only causes of parti.l di.sati.facti.n. No rejecti.n of the graft was observed. All pati.nts recovered thei. abi.i.y to penetrate wi.h no di.fi.ulty. i. addi.i.n, an i.traoperati.e average i.crease of 2.5 cm （range： 1.7-4.1 cm） i. stretched peni.e length was recorded, wi.h all pati.nts engagi.g i. penetrati.e sexual i.tercourse. i. conclusi.n, thi. procedure represents a safe and reproduci.le techni.ue for the correcti.n of peni.e curvature resulti.g from PD and yi.lds excellent cosmeti. and functi.nal results.

关键词： Egydi.'s techni.ue erecti.e dysfuncti.n graft Peyroni.'s di.ease

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Laccases from Acti.obacteri.—What We Have and What to Expect

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Advances i. Mi.robi.logy 2014年第6期4卷 285-296页

作者： Tati.na Alves Ri.amonte Fernandes Wendel Bati.ta da Si.vei.a Flávi. maria Lopes Passos Ti.go Domi.gues zucchi Embrapa Mei. Ambi.nte Rodovia SP 340JaguariúnaBrazil Departamento de Mi.robi.logi. BIOAGROUniversidade Federal de ViçosaCampus UniversitarioVicosaBrazil

Laccases are blue multi.opper enzymes, capable of oxi.i.i.g di.erse aromati. and non-aromati. compounds of i.dustri.l i.terest, concomi.antly wi.h reducti.n of molecular oxygen to water. Tolerance to extreme condi.i.ns, such as hi.h temperature, sali.i.y or extreme pH, i. requi.ed for practi.al i.dustri.l appli.ati.ns. Here we focus on bacteri.l laccases from the phylum Acti.obacteri., notably the order Acti.omycetales. Currently, less than 10 enzymes have been properly characteri.ed, all belongi.g to genus Streptomyces, but i. i. noteworthy that all of them have exhi.i.ed i.dustri.lly i.portant properti.s. Furthermore, studi.s wi.h enzymes from thi. phylum revealed a novel molecular structure of laccases, provi.i.g the basi. for a di.ti.ct fami.y, the two-domai. laccases. The relevant trai.s of acti.omycetes laccases emphasi.e the need for more studi.s i.volvi.g the i.olati.n of thi. bacteri.l group from li.ni.-ri.h envi.onmental samples, detecti.n of thei. laccase acti.i.y and thereafter, characteri.ati.n of the protei.s and related genes. The nonhomogeneous responses of acti.omycetes laccases to tradi.i.nal i.hi.i.ors, substrates or metal i.ns have challenged the currently accepted “laccase concept”. Fi.ally, consi.eri.g that di.ti.gui.hi.g laccase acti.i.y i. vi.ro from other li.ni.olyti. enzymes becomes a di.fi.ult task due to overlaps i. catalyti.al properti.s of the enzymes, we proposed a si.ple flow chart to help experi.ental assays.

关键词： Acti.omycetes Li.ni. Degradati.n Multi.opper Oxi.ase Laccases

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古尔德卡海岛的琼汉斯原工业区改造,威尼斯,意大利

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世界建筑 2005年第9期 42-45页

作者： CZA Ci.o zucchi Archi.etti.(Mi.an) CZA契诺.祖奇建筑事务所米兰

古尔德卡岛的城市布局有两种不同的尺度:岛的北边楼房林立,而另一边的工业区则远眺拉古纳海滩,且布局松散.这项工程口袋是在原工业区上进行的一种'微观手术',放弃规模较大的改造(刷新旧建筑和新建建筑),而采取了对原有建筑及空地的小规... 详细信息

古尔德卡岛的城市布局有两种不同的尺度:岛的北边楼房林立,而另一边的工业区则远眺拉古纳海滩,且布局松散.这项工程口袋是在原工业区上进行的一种'微观手术',放弃规模较大的改造(刷新旧建筑和新建建筑),而采取了对原有建筑及空地的小规模的修缮.

关键词：工业区改造古尔德威尼斯意大利海岛城市布局新旧建筑小规模海滩楼房

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糖化作用刺激体外重建全层皮肤模型中单核细胞分化

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中华皮肤科杂志 2018年第2期51卷 166-167页

作者： Pageon H zucchi H Rousset F 欧莱雅法国研发和创新中心

晚期糖化终末产物（AGEs）是由蛋白质或脂质与糖（如葡萄糖和核糖）通过非酶糖化反应形成的，是公认与自然老化相关机制之一。皮肤生物学研究认为，糖化作用对与真皮基质分子影响皮肤老化的硬化有关，可能是由改变分子间交联所致；有研... 详细信息

晚期糖化终末产物（AGEs）是由蛋白质或脂质与糖（如葡萄糖和核糖）通过非酶糖化反应形成的，是公认与自然老化相关机制之一。皮肤生物学研究认为，糖化作用对与真皮基质分子影响皮肤老化的硬化有关，可能是由改变分子间交联所致；有研究在糖尿病和老化皮肤中检测到AGEs蓄积。有文献报道，AGE沉积可使皮肤生物化学特性和功能均发生变化。AGEs通过改变细胞外基质和细胞内蛋白质，可影响皮肤自稳态和皮肤常驻细胞的功能。

关键词：皮肤模型糖化作用细胞分化体外重建晚期糖化终末产物细胞内蛋白质单核全层

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