检索结果-南通市图书馆

SmartFlare^(TM) is a reliable method for assessing mRNA expression in single neural stem cells

World Journal of Stem Cells 2021年第12期13卷 1918-1927页

作者： Andrea Diana maria dolores setzu Zaal Kokaia Roxana Nat Cristina Maxia Daniela Murtas Department of Biomedical Sciences University of CagliariMonserrato 09042CagliariItaly Laboratory of Stem Cells&Restorative Neurology Lund Stem Cell CenterLund UniversityLund SE-22184LundSweden Institute of Neuroscience Medical University of InnsbruckInnsbruck 6020Austria

BACKGROUND One of the most challenging tasks of modern biology concerns the real-time tracking and quantification of mRNA expression in living cells.On this matter,a novel platform called SmartFlare^(TM) has taken advantage of fluorophore-linked nanoconstructs for targeting RNA transcripts.Although fluorescence emission does not account for the spatial mRNA distribution,NanoFlare technology has grown a range of theranostic applications starting from detecting biomarkers related to diseases,such as cancer,neurodegenerative pathologies or embryonic developmental disorders.AIM To investigate the potential of SmartFlare^(TM) in determining time-dependent mRNA expression of prominin 1(CD133)and octamer-binding transcription factor 4(OCT4)in single living cells through differentiation.METHODS Brain fragments from the striatum of aborted human fetuses aged 8 wk postconception were processed to obtain neurospheres.For the in vitro differentiation,neurospheres were gently dissociated with Accutase solution.Single cells were resuspended in a basic medium enriched with fetal bovine serum,plated on poly-L-lysine-coated glass coverslips,and grown in a lapse of time from 1 to 4 wk.Live cell mRNA detection was performed using SmartFlare^(TM) probes(CD133,Oct4,Actin,and Scramble).All the samples were incubated at 37°C for 24 h.For nuclear staining,Hoechst 33342 was added.SmartFlare^(TM) CD133-and OCT4-specific fluorescence signal was assessed using a semiquantitative visual approach,taking into account the fluorescence intensity and the number of labeled cells.RESULTS In agreement with previous PCR experiments,a unique expression trend was observed for CD133 and OCT4 genes until 7 d in vitro(DIV).Fluorescence resulted in a mixture of diffuse cytoplasmic and spotted-like pattern,also detectable in the contacting neural branches.From 15 to 30 DIV,only few cells showed a scattered fluorescent pattern,in line with the differentiation progression and coherent with mRNA downregulation of these stemness-related genes.CONCLUSION SmartFl

关键词： mRNA detection SmartFlareTM NanoFlare Live staining Nanotechnology Neural stem cell genes

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Male genitourinary schistosomiasis-related symptoms among long-term Western African migrants in Spain: a prospective population-based screening study

引用

贫困所致传染病(英文) 2024年第2期13卷 1-10页

作者： Roure Sílvia Vallès Xavier Pérez-Quílez Olga López-Mu?oz Israel Chamorro Anna Abad Elena Valerio Lluís Soldevila Laura Espa?a Sergio Hegazy Alaa H. A. Fernández-Rivas Gema Gorriz Ester Herena dolores Oliveira Mário Miralles maria Carme Conde Carmen Montero-Alia Juan José Fernández-Pedregal Elia Miranda-Sánchez Jose Llibre Josep M. Isnard Mar Bonet Josep maria Estrada Oriol Prat Núria Clotet Bonaventura The Schisto-Stop study group Primary Health Care Unit Mataró-(Rocafonda-Palau) Barcelona North Metropolitan Health Directorate Catalan Institute for Health Camí Ral el Ravalet Mataró Barcelona Spain Barcelona North Metropolitan Primary Care Directorate Catalan Institute for Health Ctra. de Barcelona Sabadell Barcelona Spain Directorate for Innovation and Interdisciplinary Cooperation Barcelona North Metropolitan Health Directorate Catalan Institute for Health C/Can Ruti S/N Badalona Spain IrsiCaixa-AIDS Research Institute Hospital Universitari Germans Trias i Pujol University Hospital c/Can Ruti s/n Badalona Spain Infectious Diseases Directorate Barcelona North Metropolitan Health Directorate Catalan Institute for Health C/Can Ruti S/N Badalona Spain International Health Program (PROSICS) Barcelona North Metropolitan Territorial Directorate for Infectious Diseases Catalan Institute for Health CAP La Salut Passatge dels Encants S/N Badalona Spain Fundació Lluita contra les Infeccions c/Can Ruti s/n Badalona Spain Infectious Diseases Department Hospital Universitari Germans Trias i Pujol c/Can Ruti s/n Badalona Spain Germans Trias i Pujol Research Institute c/Can Ruti s/n Badalona Spain Microbiology Department Germans Trias i Pujol University Hospital c/Can Ruti s/n Badalona Spain Department of Genetics and Microbiology Universitat Autònoma de Barcelona c/Can Ruti s/n Badalona Spain Faculty of Medicine University of Asyut El Fateh Assiut Governorate Egypt Department of Urology Germans Trias i Pujol University Hospital c/Can Ruti s/n Badalona Spain Canovelles Primary Health Care Unit Barcelona North Metropolitan Health Directorate Catalan Institute for Health C/Indústria Canovelles Spain

Background Schistosomiasis is highly endemic in sub-Saharan Africa and frequently imported to Europe. Male urogenital manifestations are often neglected. We aimed to ascertain the prevalence of genitourinary clinical signs and symptoms among long-term African migrants in a non-endemic European country using a serology test.Methods We carried out a prospective, community-based cross-sectional study of adult male migrants from sub-Saharan Africa living in Spain.Schistosoma serology tests and microscopic urine examinations were carried out, and clinical data were obtained from an electronic medical record search and a structured questionnaire.Results We included 388 adult males, mean age 43.5 years [Standard Deviation (SD) = 12.0, range: 18–76]. The median time since migration to the European Union was 17 [Interquartile range (IQR): 11–21] years. The most frequent country of origin was Senegal (N = 179, 46.1%). Of the 338, 147 (37.6%) tested positive forSchistosoma. Parasite eggs were present in the urine of only 1.3%. Nine genitourinary clinical items were significantly associated with positiveSchistosoma serology results: pelvic pain (45.2%;OR = 1.57, 95%CI: 1.0–2.4), pain on ejaculation (14.5%;OR = 1.85, 95%CI: 1.0–3.5), dyspareunia (12.4%;OR = 2.45, 95%CI: 1.2–5.2), erectile dysfunction (9.5%;OR = 3.10, 95%CI: 1.3–7.6), self-reported episodes of infertility (32.1%;OR = 1.69, 95%CI: 1.0–2.8), haematuria (55.2%;OR = 2.37, 95%CI: 1.5–3.6), dysuria (52.1%;OR = 2.01, 95%CI: 1.3–3.1), undiagnosed syndromic STIs (5.4%), and orchitis (20.7%;OR = 1.81, 95%CI: 1.0–3.1). Clinical signs tended to cluster.Conclusions Urogenital clinical signs and symptoms are prevalent among male African long-term migrants with a positiveSchistosoma serology results. Genital involvement can be frequent even among those with long periods of non-residence in their sub-Saharan African countries of origin. Further research is needed to develop diagnostic tools and validate therapeutic approaches to chronic schistosomiasis.

关键词： Schistosomiasis Chronic schistosomiasis Urogenital schistosomiasis Male genital schistosomiasis Longterm migrant

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Integration of Sequence Stratigraphic Analysis and 3D Geostatistical Modeling of Pliocene–Pleistocene Delta,F3 Block,Netherlands

引用

Acta Geologica Sinica(English Edition) 2023年第1期97卷 256-268页

作者： Haris Ahmed KHAN Ali Asghar SHAHID Muhammad Jahangir KHAN Taher ZOUAGHI maria dolores ALVAREZ Syed Danial Mehdi NAQVI Hydrocarbon Development Institute of Pakistan Plot#18Street#6H-9/1IslamabadPakistan Department of Geoscience University of OsloSem Sælands vei 10371 OsloNorway Department of Earth and Environmental Sciences Bahria UniversityKarachi Campus13 National Stadium RoadKarachi-75260Pakistan Department of Geoexploration Faculty of Earth SciencesKing Abdulaziz University21589 JeddahSaudi Arabia Laboratoire de Géoressources CERTEPôle Technologique de Borj Cédria8020 SolimanTunisia Argentina's Geological and Mining Survey Aveneida General Paz 5445Edificio 25INTIGeneral San MartinArgentina

This research is focused on the analysis of the sequence stratigraphic units of F3 Block,within a wave-dominated delta of Plio–Pleistocene age.Three wells of F3 block and a 3D seismic data,are utilized in this research.The conventional techniques of 3D seismic interpretation were utilized to mark the 11 surfaces on the seismic section.Integration of seismic sequence stratigraphic interpretation,using well logs,and subsequent 3D geostatistical modeling,using seismic data,aided to evaluate the shallow hydrocarbon traps.The resulting models were obtained using System Tract and Facies models,which were generated by using sequential stimulation method and their variograms made by spherical method,moreover,these models are validated via histograms.The CDF curve generated from upscaling of well logs using geometric method,shows a good relation with less percentage of errors(1 to 2 for Facies and 3 to 4 for System Tract models)between upscaled and raw data that complements the resulted models.These approaches help us to delineate the best possible reservoir,lateral extent of system tracts(LST and/or HST)in the respective surface,and distribution of sand and shale in the delta.The clinoform break points alteration observed on seismic sections,also validates the sequence stratigraphic interpretation.The GR log-based Facies model and sequence stratigraphy-based System Tract model of SU-04-2 showed the reservoir characteristics,presence of sand bodies and majorly LST,respectively,mainly adjacent to the main fault of the studied area.Moreover,on the seismic section,SU-04-2 exhibits the presence of gas pockets at the same location that also complements the generated Facies and System Tract models.The generated models can be utilized for any similar kind of study and for the further research in the F3 block reservoir characterization.

关键词： sequence stratigraphy facies modeling system tract modeling F3 block North Sea

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Crop phenotyping in a context of global change:What to measure and how to do it

引用

Journal of Integrative Plant Biology 2022年第2期64卷 592-618页

作者： Jose Luis Araus Shawn Carlisle Kefauver Omar Vergara-Díaz Adrian Gracia-Romero Fatima Zahra Rezzouk Joel Segarra maria Luisa Buchaillot Melissa Chang-Espino Thomas Vatter Rut Sanchez-Bragado JoséArmando Fernandez-Gallego maria dolores Serret Jordi Bort Integrative Crop Ecophysiology Group Plant Physiology SectionFaculty of BiologyUniversity of BarcelonaBarcelonaSpain AGROTECNIO Center LleidaSpain Plant Ecophysiology and Metabolism Lab ITQB NovaPlant Sciences DivisionOeirasPortugal Programa de Ingeniería Electrónica Facultad de IngenieríaUniversidad de IbaguéIbaguéColombia

High-throughput crop phenotyping,particularly under field conditions,is nowadays perceived as a key factor limiting crop genetic advance.Phenotyping not only facilitates conventional breeding,but it is necessary to fully exploit the capabilities of molecular breeding,and it can be exploited to predict breeding targets for the years ahead at the regional level through more advanced simulation models and decision support systems.In terms of phenotyping,it is necessary to determined which selection traits are relevant in each situation,and which phenotyping tools/methods are available to assess such traits.Remote sensing methodologies are currently the most popular approaches,even when lab-based analyses are still relevant in many circumstances.On top of that,data processing and automation,together with machine learning/deep learning are contributing to the wide range of applications for phenotyping.This review addresses spectral and red-green-blue sensing as the most popular remote sensing approaches,alongside stable isotope composition as an example of a lab-based tool,and root phenotyping,which represents one of the frontiers for field phenotyping.Further,we consider the two most promising forms of aerial platforms(unmanned aerial vehicle and satellites)and some of the emerging data-processing techniques.The review includes three Boxes that examine specific case studies.

关键词： crop phenotyping deep learning models photosynthesis platforms remote sensing roots satellites sensors stable isotopes

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Breeding effects on the genotype×environment interaction for yield of durum wheat grown after the Green Revolution:The case of Spain

引用

The Crop Journal 2020年第4期8卷 623-634页

作者： Fadia Chairi Nieves Aparicio maria dolores Serret José Luis Araus Section of Plant Physiology University of BarcelonaBarcelonaSpain AGROTECNIO(Center of Research in Agrotechnology) LleidaSpain Instituto de Tecnología Agraria de Castilla y León(ITACyL) ValladolidSpain

This study evaluates the changes caused by breeding in the genotype by environment(G×E)interaction of the durum wheat varieties most widely cultivated in Spain after the Green Revolution.A set of 12 cultivars was tested in 27 environments,which are understood as the combination of different sites,years,and treatments(water regime and planting dates),representative of the durum wheat growing conditions in Spain with average grain yields(GY)ranging between 2.8 and 9.1 Mg ha^-1.The most important environmental factors affecting the G×E interaction for yield were themaximum and the mean temperature during the entire crop cycle.An improvement in genetic yield was observed in warm environments and under optimal water conditions that resemble those where the germplasm originated(essentially as advanced lines)before its release in Spain.Therefore,the adaptation of semi-dwarf durum in Spain has shown a tendency to specific adaptation rather than large-scale adaptation.Two different patterns of selection have been reported due the G×E interaction and changes in the ranking of genotypes:in the high yielding environments(GY>5 Mg ha^-1),plants favor increased water uptake,with higher levels of transpiration and more open stomata(more negative values of carbon isotope composition,δ^13 C,and higher canopy temperature depression,CTD),whereas,in low yielding environments(GY<5 Mg ha^-1)plants close their stomata and favor greater water use efficiency(less negativeδ^13 C values and lower CTD values).

关键词： breeding cultivar dwarf

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Difficulty scores in laparoscopic liver surgery—getting closer to a powerful and necessary tool

引用

Hepatobiliary Surgery and Nutrition 2019年第4期8卷 428-430页

作者： Ruben Ciria maria dolores Ayllon Javier Brice(n)o Unit of Hepatobiliary Surgery and Liver Transplantation University Hospital Reina Sofia Cordoba Spain

Obtaining standardized difficulty scores for surgical techniques is mandatory. The more accurate the scores become, the better results may be achieved in terms of patients' safety and surgical implementation. This need is especially useful when technically demanding and complex surgical procedures are performed. In this sense, liver resections are difficult procedures with high morbidity and mortality rates that should be performed by experienced surgeons. Hepatobiliary surgery has always been considered as a long-term project that needs patience, expertise and a progressive learning curve. Since the first reported laparoscopic liver resection in 1996, the adoption and implementation of minimally invasive liver surgery has been cautious. However, laparoscopic liver resections have experienced a wide expansion in recent years (1), mainly after the last Consensus Meeting held in Morioka in 2014 (2). One of the statements reported during this Consensus and in the latter European Guidelines Meeting held in Southampton in 2017 (3) is that a need for a safe expansion and control on technical procedures should be encouraged. In this context, liver surgical teams are trying to develop feasible tools that may standardize laparoscopic liver resections.

关键词： surgery closer latter

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Gene expression and physiological responses to salinity and water stress of contrasting durum wheat genotypes

引用

Journal of Integrative Plant Biology 2016年第1期58卷 48-66页

作者： Salima Yousfi Antonio J.Marquez Marco Betti Jose Luis Araus maria dolores Serret Unit of Plant Physiology Department of Plant BiologyFaculty of BiologyUniversity of Barcelona08028Spain Department of Plant Biochemistry and Molecular Biology Faculty of ChemistryUniversity of SevilleSevilla 41012Spain

Elucidating the relationships between gene expression and the physiological mechanisms remains a bottleneck in breeding for resistance to salinity and drought. This study related the expression of key target genes with the physiological performance of durum wheat under different combinations of salinity and irrigation. The candidate genes assayed included two encoding for the DREB（dehydration responsive element binding） transcription factors Ta DREB1 A and Ta DREB2 B, another two for the cytosolic and plastidic glutamine synthetase（Ta GS1 and Ta GS2）, and one for the specific Na^＋/H^＋ vacuolar antiporter（Ta NHX1）. Expression of these genes was related to growth and different trait indicators of nitrogen metabolism（nitrogen content, stable nitrogen isotope composition, and glutamine synthetase and nitrate reductase activities）, photosynthetic carbon metabolism（stable carbon isotope composition and different gas exchange traits） and ion accumulation. Significant interaction between genotype and growing conditions occurred for growth, nitrogen content, and the expression of most genes.In general terms, higher expression of Ta GS1, Ta GS2,Ta DREB2 B, and to a lesser extent of Ta NHX1 were associated with a better genotypic performance in growth, nitrogen, and carbon photosynthetic metabolism under salinity and water stress. However, Ta DREB1 A was increased in expression under stress compared with control conditions, with tolerant genotypes exhibiting lower expression than susceptible ones.

关键词： Durum wheat （Triticum turgidum ssp. durum） geneexpression nitrogen metabolism salinity stable isotope

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Selective uptake of major and trace elements in Erica andevalensis,an endemic species to extreme habitats in the Iberian Pyrite Belt

引用

Journal of Environmental Sciences 2011年第3期23卷 444-452页

作者： Fabrizio Monaci Eduardo O.Leidi maria dolores Mingorance Benito Valdés Sabina Rossini Oliva Roberto Bargagli Department of Environmental Sciences"G.Sarfatti" University of Siena Via P.A.Mattioli4Siena 53100Italy Department of Plant Biotechnology IRNAS-CSICAvda.Reina Mercedes10Sevilla 41012Spain Andalusian Institute of Earth Science CSIC-UGRProf.Albareda1Granada 18008Spain Deparment of Plant Biology and Ecology University of SevilleAvda.Reina Mercedes s/nApartado de Correo 1095Sevilla 41080Spain

To assess the ecophysiological traits and the phytoremediation potential of the endemic heather Erica andevalensis, we determined the concentrations of major and trace elements in different plant parts and in rizosphere soils from Riotinto mining district （Huelva, Spain）. The results showed that E. andevalensis may grow on substrates with very high As, Cu, Fe and Pb concentrations （up to 4114, 1050, 71900 and 15614μg/g dry weight, respectively）, very low availability of macro- and micronutrients and with pH values ranging from 3.3 to 4.9. In these harsh edaphic conditions E. andevalensis selectively absorbed and translocated essential nutrients and excludes potentially phytotoxic elements, which were accumulated in the root epidermis. The concentrations of major and trace elements in E. andevalensis aerial parts from the Riotinto mining district were in the normal range for plants; likewise other Erica species it accumulated Mn and only in a very polluted site we measured leaf concentrations of As and Pb within the excessive or toxic limits for plants. Differently from previous studies, which emphasized the soil pH and bioavailability of phytotoxic elements as the main stress factors, this study showed that in the Riotinto region, E. andevalensis can tolerate wide range of pH and toxic element concentrations; the harshest environments colonized by monospecific patches of this species were characterized above all by very low availability of nutrients. The extraordinary capability to adapt to these extreme habitats made E. andevalensis a priority species to promote the phytostabilization and the development of a self-sustaining vegetative cover on Riotinto mine tailings.

关键词： mine tailing Riotinto tolerant plants Erica andevalensis rhizosphere soils

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Wheat ear carbon assimilation and nitrogen remobilization contribute significantly to grain yield

引用

Journal of Integrative Plant Biology 2016年第11期58卷 914-926页

作者： Bangwei Zhou maria dolores Serret Abdelhalim Elazab Jordi Bort Pie José Luis Araus Iker Aranjuelo álvaro Sanz-Sáez Key Laboratory of Vegetation Ecology Ministry of EducationInstitute of Grassland ScienceNortheast Normal University Unit of Plant Physiology Faculty of BiologyUniversity of Barcelona Agrobiotechnology Institute Public University of Navarra-CSIC-Navarra GovernmentArrosadia Campus Department of Plant Biology University of the Basque Country Pharmacy Faculty

The role of wheat ears as a source of nitrogen （N） and carbon （C） in the grain filling process has barely been studied. To resolve this question, five wheat genotypes were labeled with 15N-enriched nutrient solution. N remobilization and absorption were estimated via the nitrogen isotope composition of total organic matter and Rubisco. Gas exchange analyses showed that ear photosynthesis contributed substantially to grain filling in spite of the great loss of C due to respiration. Of the total kernel N, 64.7% was derived from the N acquired between sowing and anthesis, while the remaining 35.3% was derived from the N acquired between anthesis and maturity. In addition, 1.87 times more N was remobilized to the developing kernel from the ear than from the flag leaf. The higher yielding genotypes showed an increased N remobflization to the kernel compared to the lower yielding genotypes. In addition, the higher yielding genotypes remobilized more N from the ears to the kernel than the lower yielding genotypes, while the lower yielding genotypes remobilized more N from the flag leaf to the kernel. Therefore, the ears contribute significantly toward fulfilling C and N demands during grain filling.

关键词： Carbon accumulation ear grain yield nitrogenaccumulation Rubisco

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Is there a relationship between dopamine and rhegmatogenous retinal detachment?

引用

Neural Regeneration Research 2020年第2期15卷 311-314页

作者： Alessio Martucci Massimo Cesareo maria dolores Pinazo-Durán Michela Di Pierro Matteo Di Marino Carlo Nucci Massimiliano Coletta Raffaele Mancino Ophthalmology Unit Department of Experimental MedicineUniversity of Rome Tor VergataRomeItaly Ophthalmic Research Unit "Santiago Grisolia"/FISABIO and Cellular-Molecular Ophthalmobiology Group/University of Valencia ValenciaSpain Department of Clinical Sciences and Translational Medicine University of Rome Tor VergataRomeItaly

Dopamine and its receptors have been widely studied in the neurological conditions and in the retina. In this study, we evaluated the possible role of dopamine in rhegmatogenous retinal detachment(RRD) by comparing the amount of 3,4-dihydroxyphenylacetic acid(DOPAC), a surrogate index of retinal dopamin levels, in the vitreous sample of patients affected by RRD with those affected by macular pucker and vitre ous hemorrhage. Our results showed that significantly higher levels of DOPAC were found in the vitreou sample of patients affected by RRD compared with those affected by vitreous hemorrhage and macula pucker(P = 0.002). Specifically, no trace of the substance was found in vitreous hemorrhage and macula pucker samples. A slightly significant positive correlation was found among DOPAC and post-operativ best corrected visual acuity(r = 0.470, P = 0.049). No correlation was found between DOPAC and the day elapsed between diagnosis and surgery(P = 0.317). For the first time our findings suggest that DOPAC i released in RRD, but not in other retinal diseases such as vitreous hemorrhage and macular pucker. More over, we showed a correlation between visual acuity outcome and the amount of DOPAC in the vitreous This might have a potential, although still unknown, implication in the pathogenesis of the disease and/o in the associated photoreceptors loss. This study was approved by the Ethics Committee of Rome Tor Ver gata University Hospital(R.S.92.10) on September 24, 2010.

关键词： 3,4-dihydroxyphenylacetic acid DOPAC dopamine dopaminergic amacrine cells dopaminergic neurotoxicity macular pucker oxidative stress photoreceptor degeneration rhegmatogenous retinal detachment vitreous hemorrhage

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：