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RNAi technology targeting PbGP43 and PbP27 in Paracoccidioides brasiliensis

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Open Journal of Genetics 2013年第2期3卷 1-8页

作者： Isaura Torres Gomez Orville Hernandez Ruiz Jose F.Munoz Ana maria Garcia Angela Restrepo Juan G.McEwen Unidad de Biologia Celular y Molecular Corporacion para Investigaciones Biologicas(CIB)MedellinColombia Instituto de Biologia Universidad de AntioquiaMedellinColombia Grupo de Investigacion en Biociencias Facultad de Ciencias de la SaludInstitucion Universitaria Colegio Mayor de AntioquiaMedellinColombia Facultad de Medicina Universidad de AntioquiaMedellinColombia

Efficient technologies for gene silencing would be important to carry out functional analysis with P. brasiliensis genes, as well as for a better understanding of the biology and pathogenesis of this pathogenic fungus. Due to the fact that homologous recombination is unusual in P. brasiliensis, the development of knockout isolates is currently non-feasible. The goal of this work was to assess RNA interference (RNAi) technology as an alternative tool for gene silencing previously employed successfully in H. capsulatum. For this purpose, we built different inverted repeat transgenic hairpin constructs to down-regulate the PbGP43 and PbP27 genes known to codify for two fungal immunogenic proteins that elicit a strong immune response during experimental paracoccidioidomycosis. Using the RNAi strategy, a reduction in the mRNA levels of the PbGP43 and PbP27 genes was observed during the first 20 days after selection;however, in the transformed yeast cells, the gene silencing status proved non-stable through the assay. We demonstrated that electrotransformation was suitable to transform P. brasiliensis yeast cells and integrate the hairpin constructions;nonetheless, gene silencing was not stable along the experimental time. A detailed analysis of the underlying molecular RNAi machinery may provide further insights into the intracellular mechanism that governs this reverse genetic tool.

关键词： Paracoccidioides brasiliensis Interference RNA Gene Silencing PbGP43 PbP27 Gene Expression

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转台大战

空中英语教室（初级版．大家说英语） 2012年第1期 16-16,49,50页

作者： Ruth Devlin maria Chen（page design）

10岁的凯西拿着一大碗爆米花坐在沙发上．她打开电视转到14台，她总是在下午看“英雄”这个节目．这是她最喜欢的节目。有的时候剧情挺严肃的．靠时却很好笑。不过她一直很喜欢看这个节目。

关键词：转台 “英雄” 爆米花节目剧情

Mathematical Modeling and Determination of Effective Diffusivity of Mesquite during Convective Drying

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American Journal of Plant Sciences 2016年第6期7卷 814-823页

作者： Rennan Pereira de Gusmão Thaisa Abrantes Souza Gusmão Mário Eduardo Rangel Moreira Cavalcanti-Mata maria Elita Martins Duarte Federal University of Campina Grande Campina Grande Brazil

The mesquite pods have a high nutritional value, being a rich source of sugars, proteins, minerals and fibers that can be used as a raw material for the development of a number of food products and technological innovations. The objective of this work was to study the drying kinetics of mesquite pods in a thin layer, 20 mm length and determine the effective diffusion coefficient by adjusting mathematical models that were based on heat transfer and mass fundamentals. For the experiments, we used mature mesquite, with 20% water content (b.u). Drying experiments were performed using temperatures of 50°C, 60°C, 70°C and 80°C with drying air speed of 2 m•s-1. It was used a convective dryer with fixed bed and upflow air. Mathematical models of Fick, Page, Cavalcanti-Mata, Two Exponential Terms and Henderson & Pabis were used to adjust the experimental data. To calculate the effective diffusivity, flat plate geometry with sample thickness of 6.5 mm was used. Based on the results obtained, it was concluded that the loss of water from mesquite occurred during the drying period in decreasing rate;and with increasing drying temperature, total process time was reduced. The diffusion coefficient increased by increasing drying temperature. Mathematical models including theoretical (Fick), semi-theoretical (Page and Cavalcanti-Mata) and empirical (Two Exponential Terms) models satisfactorily explain the experimental data of drying mesquite.

关键词： Diffusion Coefficient Prosopis juliflora Subproduct

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新的人轮状病毒株的分离

微生物学免疫学进展 1986年第4期 23-24页

作者： Anna maria Agllano ,李玉如

人轮状病毒在任何培养系统中繁殖是很困难的。1980年Whyatt等经过在悉生小猪传代几次后获得了适应组织培育生长的人轮状病毒“Wa”株。1981年Sato和Urasawa等在细胞维持液中加入胰酶并使用旋转培养,直接从人粪便中分离到轮状病毒。1983... 详细信息

人轮状病毒在任何培养系统中繁殖是很困难的。1980年Whyatt等经过在悉生小猪传代几次后获得了适应组织培育生长的人轮状病毒“Wa”株。1981年Sato和Urasawa等在细胞维持液中加入胰酶并使用旋转培养,直接从人粪便中分离到轮状病毒。1983年Birch等报导采用胰酶预处理粪样提取液,维持液中加入胰酶的旋转培养。成功地在HA—104和CV—1细胞上适应几株人轮状病毒。

关键词：人轮状病毒胰酶旋转培养纤维细胞人胚细胞培养 LLC 培养(生物) 电镜检查粪样

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多吃蔬菜!

空中英语教室（初级版．大家说英语） 2022年第11期 42-44,55,56页

作者： Ruth Devlin maria CHEN(PAGE DESIGN) 不详

你喜欢蔬菜吗?蔬菜对你的身体有益。蔬菜也很美味。有些人喜欢生菜,他们会在沙拉当中吃生菜、番茄与西蓝花。沙拉里面放卷心菜也很好吃,有些人会在沙拉里放洋葱。生胡萝卜则是健康的零食。一般人也会烹煮蔬菜。你可以用许多方式料理土... 详细信息

你喜欢蔬菜吗?蔬菜对你的身体有益。蔬菜也很美味。有些人喜欢生菜,他们会在沙拉当中吃生菜、番茄与西蓝花。沙拉里面放卷心菜也很好吃,有些人会在沙拉里放洋葱。生胡萝卜则是健康的零食。一般人也会烹煮蔬菜。你可以用许多方式料理土豆和玉米,新鲜玉米很美味。

关键词：卷心菜零食生菜美味胡萝卜蔬菜料理沙拉

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纠正膀胱输尿管返流的一种新手术方法

国际泌尿系统杂志 1984年第6期04卷 272-274页

作者： Jose maria Gil-Vernet 林冰若

20多年来,内外科处理膀胱输尿管返流是小儿泌尿科的一个大的问题。提出与应用了许多有效的手术方法。这里报道一种新的手术技术,特别对巨大三角区的病人有效,曾应用于38例儿童与成人获得优良结果。这种技术包括一针缝合使三角区迭盖,有... 详细信息

20多年来,内外科处理膀胱输尿管返流是小儿泌尿科的一个大的问题。提出与应用了许多有效的手术方法。这里报道一种新的手术技术,特别对巨大三角区的病人有效,曾应用于38例儿童与成人获得优良结果。这种技术包括一针缝合使三角区迭盖,有效地延长了输尿管终端的壁内段。

关键词：输尿管三角区小儿泌尿泌尿生殖系统膀胱输尿管返流膀胱疾病手术方法

Chronic hepatitis C:This and the new era of treatment

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World Journal of Hepatology 2016年第2期8卷 92-106页

作者： Gaetano Bertino Annalisa Ardiri maria Proiti Giuseppe Rigano Evelise Frazzetto Shirin Demma mariaIrene Ruggeri Laura Scuderi Giulia Malaguarnera Nicoletta Bertino Venerando Rapisarda Isidoro Di Carlo Adriana Toro Federico Salomone mariano Malaguarnera Emanuele Bertino Michele Malaguarnera Hepatology Unit-Department of Clinical and Experimental Medicine University of Catania95123 CataniaItaly Internal Medicine Unit ARNAS Civic Hospital90142 PalermoItaly Research Centre“La Grande Senesce” University of Catania95100 CataniaItaly Department of Biomedical Sciences University of Catania95100 CataniaItaly Faculty of Pharmacy University of Catania95123 CataniaItaly Occupational Medicine Unit Department of Clinical and Experimental MedicineUniversity of Catania95100 CataniaItaly Department of Surgical Sciences Organ Transplantation and Advanced TechnologiesUniversity of Catania95100 CataniaItaly Gastroenterology Unit Acireale Hospital95024 AcirealeCataniaItaly

Over the last years it has started a real revolution in the treatment of chronic hepatitis C. This occurred for the availability of direct-acting antiviral agents that allow to reach sustained virologic response in approximately 90% of cases. In the near future further progress will be achieved with the use of pan-genotypic drugs with high efficacy but without side effects.

关键词： Direct-acting antiviral agents Nucleosideinhibitors Boceprevir Sofosbuvir Telaprevir HepatitisC Simeprevir Daclatasvir Ledipasvir Faldaprevir Ritonavir Ombitasvir Dasabuvir

Correlation between Endocervical Length in the First Trimester and Spontaneous Preterm Delivery

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Open Journal of Obstetrics and Gynecology 2021年第11期11卷 1608-1618页

作者： Korine Camargo de Oliveira mariana Menegon de Souza Patricia Telló Dürks maria Alexandrina Zanatta Eduardo Becker Jr. Janete Vettorazzi Post Graduation Program in Health Sciences: Gynecology and Obstetrics Faculdade de Medicina da Universidade Federal do Rio Grande do Sul (UFRGS) RS Brazil Service of Gynecology and Obstetrics Hospital Moinhos de Vento Porto Alegre Porto Alegre RS Brazil Ecofetal-Ultrasound Clinic Porto Alegre RS Brazil Pediatrics Service of Moinhos de Vento Hospital Porto Alegre RS Brazil Service of Gynecology and Obstetrics Hospital de Clinicas de Porto Alegre Porto Alegre Porto Alegre RS Brazil

Introduction: Prematurity is a public health problem in Brazil, with 12% of deliveries occurring before 37 weeks of gestation. The measurement of the cervix in the second trimester is already established as a method of screening for prematurity and some studies point out advantages to start this screening in the first trimester. Objective: To define the correlation between the length of the endocervix by transvaginal ultrasound in the first trimester (11 to 14 weeks) with spontaneous early deliveries. Method: A prospective and observational study realized in a suplementar and private ultrasound clinic and hospital of Porto Alegre, Brazil between 2019-2020. Ultrasound screening of cervix was performed in singleton pregnancies in first and second trimester of pregnancy and correlated with age of delivery. Results: 142 pregnant women were studied, 80% were in the first pregnancy. The average age was 33.8 years. The rate of prematurity was 18% before 37 weeks and 4% before 34 weeks. The average of cervix measured in the first and second trimesters in deliveries before 34 weeks was 32.7 mm and 29.3 mm, respectively. In term deliveries the median cervical length was 38.8 mm and 37.8 mm, respectively. When analyzing the measurements of the cervix in the second trimester, the cervix was smaller (p = 0.008) among deliveries below 34 weeks (29 mm) than deliveries after 37 weeks. No statistically related differences were found between preterm birth and first

关键词： Preterm Birth Screening Cervical Length Measurement Preterm Birth Prevention Clinic

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欧洲卫生纸市场通过创新仍有发展机会

生活用纸 2007年第5期8卷 7-9页

作者： maria Lindahl 周杨(编译) 陈海昌(审校)

来自零售商品牌的价格压力、大量采购、市场饱和以及零售终端的促销和折扣活动——所有这些制约发展的因素经常同时出现在欧洲卫生纸市场，这并不是毫无原因的。近年来，价格的不稳定确实困扰着卫生纸行业。例如，2004年意大利33％的卫... 详细信息

来自零售商品牌的价格压力、大量采购、市场饱和以及零售终端的促销和折扣活动——所有这些制约发展的因素经常同时出现在欧洲卫生纸市场，这并不是毫无原因的。近年来，价格的不稳定确实困扰着卫生纸行业。例如，2004年意大利33％的卫生纸产品是促销售出的，在德国，零售商品牌卫生纸产品的市场份额已上升到了空前的高度。

关键词：卫生纸行业市场份额欧洲卫生纸产品创新零售终端零售商不稳定