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Determination of the Anti-Adhesive and Anti-Biofilm Capacity of a Wheat Extract on <i>Staphylococcus aureus</i>in Farms

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Journal of Materials Science and Chemical Engineering 2021年第9期9卷 11-21页

作者： maria angeles calvo torras Esteban Leonardo Arosemena Angulo Susana Martin Orúe Xavier torras Bombardó Universitat Autònoma de Barcelona (UAB) Facultad de Veterinaria Department of Animal health and Anatomy Cerdanyola del Vallès Spain Universitat Autònoma de Barcelona (UAB) Facultad de Veterinaria Department of Animal and Food Science Cerdanyola del Vallès Spain

The formation of biofilm by pathogenic microorganisms has become a problem in the livestock industry since it is considered a potential source of infection for farm animals while increasing microbial resistance to physical and chemical agents. Some plant extracts, such as soluble wheat extract, have been shown to be effective in inhibiting or destroying the biofilm of certain micro-organisms under specific conditions. The objective of this study is to evaluate the capacity of the pathogen to form biofilm on different surfaces used in livestock, as well as to evaluate the anti-biofilm capacity of the soluble wheat extract against S. aureus on these surfaces. The inhibition potential of inhibition or destruction of biofilm was tested in vitro. Wheat extract at a concentration of 0.29 mg/100mL showed anti-biofilm activity on S. aureus, inhibiting its formation, as well as destroying it greatly after a contact time of 24 hours, on those surfaces where the microorganism presents more adhesion capacity.

关键词： Microbiology Biofilm Anti-Biofilm Wheat Extract Staphylococcus Aureus

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Microbiota from <i>Helix aspersa</i>Müller in Barcelona Area (Spain)

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Advances in Microbiology 2014年第10期4卷 604-608页

作者： Lucia Pérez Caullan Gisela Girmé Vila Esteban Arosemena Angulo Agustín calvo José Antonio Marcelo M. angeles calvo torras Universitat Autónoma de Barcelona Barcelona Spain

In recent decades snail consumption has been increasing throughout Europe. The Heliciculture meets this need and gets snail farms, in order to get consistent production in the shortest possible time. The research provided, has a main objective to establish the microbiota of Helix aspersa Müller, both from healthy animals, and individuals with gastrointestinal processes. The samples selected for the study were feces obtained from snails collected in both breeding farms as field snails. In the case, farm animals were selected apparently form healthy individuals and others were with signs of gastrointestinal process study. Aliquots of each sample were plated on a range of culture media and incubated under different conditions of temperature and respiration. From the results we can note that the healthy snails (farmed or free-living) have been isolated and identified mostly, as strains belonging to the genera Pediococcus, Lactobacillus and Lactococcus and five strains of yeasts of the genera Candida and Cryptococcus. Individuals suffering from gastrointestinal disturbances processes have been isolated in high proportion species of Klebsiella, Pantoea, Citrobacter and Enterobacter. The results obtained indicate that the snails with health problems reduce the presence of strains of Lactobacillus and Lactococcus in favor of increasing and/or establishing colonization by strains of Enterobacteriaceae.

关键词： Helix aspersa Muller Heliciculture Lactic Acid Bacteria Enterobacteriaceae

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