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Journal of Forestry Research 2018年第1期29卷 73-84页

作者： Joamir Barbosa Filho maria angélica di carvalho Leandro Silva de Oliveira Enéas Ricardo Konzen Gilvano Ebling Brondani College of Forestry Federal University of Mato Grosso(UFMT) Institute of Agronomic Sciences Federal University of Minas Gerais (UFMG) Department of Forest Sciences Federal University of Lavras (UFLA)

Khaya anthotheca is a hardwood species from Africa and recently introduced to Brazil. This species yields high-quality wood for diverse applications, but little has been done toward an effective propagation method for large-scale production in nurseries. We evaluated the effects of different concentrations of macro and micronutrients（i.e., 100, 50 and 25% of the concentration of a referenced solution） combined with indole-3-butyric acid（IBA） at 0 and 2 g L-1 on the survival rate of mini-stumps and the survival and adventitious rooting of K. anthotheca mini-cuttings. The mini-stumps were grown from a source of seeds imported from Ghana, West Africa. The ministumps survived at a high rate（97%）. Consistently high shoot yields were obtained from the mini-stumps（average of 1.8 per mini-stump）. High survival percentage of minicuttings and adventitious rooting were observed when solutions with 100 and 50% of the nutrients concentration were combined with 2 g L-1 IBA. Histological sections analyzed through optical microscopy indicated the tissues from mini-cutting stems were juvenile, which might have stimulated adventitious rooting. These results have important implications for further work aimed at establishing propagation strategies for K. anthotheca, which are of prime importance for assisting breeding programs of this species.

关键词： African mahogany Seminal mini-garden Adventitious rooting Juvenile tissue Mini-stump

Ternary supramolecular quantum-dot network flocculation for selective lectin detection

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Nano Research 2016年第7期9卷 1904-1912页

作者： maria Oikonomou Junyou Wang Rui Rijo carvalho Aldrik H. Velders Laboratory of BioNanoTechnology WageningenUR Dreijenplein 6 6703 HB Wageningen the Netherlands Micronit Microfluidics B.V. Colosseum 15 7521 PV Enschede the Netherlands Interventional Molecular Imaging Laboratory Department of Radiology Leiden University Medical Center 2333 ZA Leiden the Netherlands

We present a versatile, tuneable, and selective nanoparficle-based lectin biosensor, based on flocculation of ternary supramolecular nanoparticle networks （NPN）, formed through the sequential binding of three building blocks. The three building blocks are ^-cyclodextrin-capped CdTe quantum dots, tetraethylene glycol-tethered mannose-adamantane cross-linkers （ADTEGMan）, and the tetravalent lectin Concanavalin A （ConA）. The working principle of this selective sensor lies in the dual orthogonal molecular interactions of the linker, uniting adamantane-^-cyclodextrin and mannose-lectin interaction motifs, respectively. Only when the lectin is present, sequential binding takes place, leading to in situ self-organization of the sensor through the formation of ternary supramolecular networks. Monitoring the loss of fluorescence signal of the quantum dots in solution, caused by controlled network formation and consecutive flocculation and sedimentation, leads to selective, qualitative, and quantitative lectin detection. Fluorescent sedimented networks can be observed by the naked eye or under UV illumination for a lectin concentration of up to 10 8 M. Quantitative detection is possible at 100 min with a lower detection limit of approximately 5 × 10 ^-8 M.

关键词： supramolecular cyclodextrin quantum dot sensor lectin flocculation

Water-Based Processing of Fiberboard of Acrylic Resin Composites Reinforced With Cellulose Wood Pulp and Cellulose Nanofibrils

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Journal of Renewable Materials 2019年第5期7卷 403-413页

作者： Emanoele maria Santos Chiromito Eliane Trovatti Antonio Jose Felix carvalho Department of Materials Engineering São Carlos School of EngineeringUniversity of São PauloAv.João Dagnone110013563-120São Carlos-SP-Brazil University of Araraquara-UNIARA R.Carlos Gomes1217CEP 14801-320AraraquaraSPBrazil

Despite the great potential of cellulose wood pulp and cellulose nanofibrils as reinforcing filler in thermoplastics,its use is limited due to its tendency to form agglomerates and due to its high hydrophilic *** we describe fiberboard composites with high contents of wood pulp or cellulose nanofibrils,and a resin of poly(styrene-methyl-methacrylate-acrylic acid)used as water-based *** wood pulp and cellulose nanofibrils were used directly in the form of water *** method is based on the flocculation of the polymer emulsion followed by agglomeration of a mixture of the polymer emulsion and cellulose suspension,leading to the co-precipitation of the composite material,which can be easily separated from the water *** with acrylic polymer/cellulose fibers in the proportions of 75:25,50:50 and 25:75 wt%were *** were characterized by scanning electron microscopy(SEM),Fourier transform infrared spectroscopy(FTIR),thermogravimetric analysis(TGA),dynamic mechanical analysis(DMA)and water absorption *** analysis revealed a very good dispersion of the fibers without evidence of agglomeration,which led to superior mechanical *** results showed the effectiveness of the methodology and the potential of cellulose wood pulp and CNF as reinforcement fillers in fiberboard composites and any other high fiber-content materials.

关键词： Cellulose wood pulp cellulose nanofibrils composites acrylic resin emulsion fiberboard

Extracts of the seaweed Bifurcaria bifurcata display antifungal activity against human dermatophyte fungi

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Journal of Oceanology and Limnology 2019年第3期37卷 848-854页

作者： carvalho G.Loic SILVA Raquel GONCALVES J.maria BATISTA T.maria PEREIRA Leonel Environmental and Biochemical Sciences Group the James Hutton InstituteDundee DD2 5DAScotlandUK Institute of Mechanical Process and Energy EngineeringSchool of Engineering and Physical SciencesHeriot Watt UniversityEdinburgh EH14 4ASScotlandUK MARE (Marine and Environmental Sciences Centre) Department of Life SciencesFaculty of Sciences and TechnologyUniversity of CoimbraCoimbra 3030-790Portugal CIEPQPF Department of Chemical EngineeringFaculty of Science and TechnologyUniversity of CoimbraCoimbra 3030-790Portugal

Bifurcaria bifurcata is a seaweed of the Order Fucales(Ochrophyta,Phaeophyceae)that can be found all year round along the Portuguese Atlantic *** it is considered edible in some countries,its biotechnological potential has not yet been assessed in detail and here we report its antimicrobial potential against human dermatophyte *** samples were harvested from Baleal Island(Peniche,Portugal)harvested in winter(BbPe);from Aguda Beach(Arcozelo,Vila Nova de Gaia,Portugal),harvested in autumn(BbAg)and a sample obtained by culturing the Aguda sample under laboratory conditions(BbLC).A broth macrodilution assay was applied to determine the MIC(minimum inhibitory concentration)and MLC(minimum lethal concentration)of the *** *** show that the BbPe MeOH extracts were the most effective and had higher antifungal activity against all the tested dermatophyte strains than the BbLC and BaAg extracts[Epidermophyton floccosum FF9(100–200μg/mL MIC;200μg/mL MLC),Microsporum canis FF1(400μg/mL MIC;≥400μg/mL MLC),Trichophyton mentagrophytes FF7(100μg/mL MIC;>800μg/mL MLC),*** CECT 2908(800μg/mL MIC;≥800μg/mL MLC),*** *** CECT 2958(800μg/mL MIC;≥800μg/mL MLC),*** CECT 2794(200μg/mL MIC;≥400μg/mL MLC)].In fact,only *** FF9 was sensitive to BbAg and BbLC MeOH *** our knowledge,this is the first report of antifungal activity of *** against human dermatophyte fungi.

关键词： seaweed Bifurcaria bifurcata bioactivity antifungal dermatophytes minimum inhibitory concentration minimum lethal concentration

Pathophysiology of cystic fibrosis and drugs used in associated digestive tract diseases

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World Journal of Gastroenterology 2013年第46期19卷 8552-8561页

作者： Adriana Haack Giselle Gonalves Arago maria Rita carvalho Garbi Novaes Department of Health Universidade Paulista (UNIP)

Cystic fibrosis(CF)causes chronic infections in the respiratory tract and alters the digestive *** paper reviews the most important aspects of drug treatment and changes in the digestive tract of patients with *** is a review of the literature,emphasizing the discoveries made within the last 15 years by analyzing scientific papers published in journals indexed in the Scientific Electronic Library Online,Sciences Information,United States National Library of Medicine and Medical Literature Analysis and Retrieval System Online databases,both in English and Portuguese,using the key words:cystic fibrosis,medication,therapeutic,absorption,***,observational,experimental,and epidemiological clinical studies were selected,among others,with statistical significance of 5%.This review evaluates the changes found in the digestive tract of CF patients including pancreatic insufficiency,constipation and liver *** in nutritional status are also *** treatment,nutritional supplementation and drug management were classified in this review as essential to the quality of life of CF patients,and became available through public policies for monitoring and treating *** information gathered on CF and a multi professional approach to the disease is essential in the treatment of these patients.

关键词： Cystic fibrosis Medication Therapeutic Absorption digestion

Microfibrillated Cellulose from Sugarcane Bagasse as a Biorefinery Product for Ethanol Production

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Journal of Renewable Materials 2018年第2期6卷 195-202页

作者： Rafael Grande Eliane Trovatti maria Tereza B.Pimenta Antonio J.F.carvalho University of São Paulo Av.João Dagnone 1100CEP 13563-120São CarlosSão PauloBrazil University of Araraquara(UNIARA) R.Carlos Gomes1217CEP 14801-320AraraquaraSão PauloBrazil Suzano Papel e Celulose SA Av.Lirio Correa146506460-040LimeiraSão PauloBrazil

Research involving the preparation of microfibrillated cellulose(MFC)from sugarcane bagasse is a relevant topic to the production of new nanomaterials and more accessible cellulose substrates for the production of second generation *** the transformation of cellulose into glucose,the precursor of second generation ethanol,this nanosized cellulosic substrate represents a more appropriate material for the chemical hydrolysis *** high aspect ratio of MFC improves hydrolysis,requiring mild conditions and decreasing the generation of ***,MFC was prepared from sugarcane bagasse by ultrasound *** material was oxidized with 2,2,6,6-tetramethylpiperidine-N-oxyl(TEMPO)to produce negatively charged high defibrillated *** MFC was characterized by Fourier transform infrared spectroscopy(FTIR),X-ray diffraction(XRD),scanning electron microscopy(SEM)and viscosity measurements.

关键词： Cellulose nanofibrils substrate second generation ethanol

Enterococcus faecalis can be distinguished from Enterococcus faecium via differential susceptibility to antibiotics and growth and fermentation characteristics on mannitol salt agar

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Frontiers in Biology 2012年第2期7卷 167-177页

作者： maria L. G. QUILOAN John VU John carvalho Biology Program Biology Department California State University Dominguez Hills （CSUDH） Carson CA 90747 USA M. S. Biology Program Biology Department California State University Dominguez Hills （CSUDH） Carson CA 90747 USA Biology Department California State University Dominguez Hills （CSUDH） Carson CA 90747 USA

Enterococcus faecalis and Enterococcus faecium are both human intestinal colonizers frequently used in medical bacteriology teaching laboratories in order to train students in bacterial identification. In addition, hospitals within the United States and around the world commonly isolate these bacteria because they are a cause of bacteremia, urinary tract infections, endocarditis, wound infections, and nosocomial infections. Given that enterococci are becoming more of a world health hazard, it is important for laboratories to be able to distinguish these bacteria within hospitalized patients from other bacterial genera. In addition, laboratories must differentiate different species within the Enterococcus genus as well as different strains within each species. Though enterococci are differentiated from other bacterial genera via classical culture and biochemical methods, nucleic acid sequencing is required to differentiate species within the genus--a costly, time consuming, and technically challenging procedure for laboratory technicians that, in itself, does not necessarily lead to speedy identification of bactericidal antibiotics. In this study, we perform antibiogram analysis to show （1） that penicillin can be rapidly employed to distinguish strains and clinical isolates of E. faecalis from E. faecium, （2） E. faecalis is susceptible to ampicillin, and （3） that vancomycin resistance in enterococci shows no sign of abating. Additionally, we show that E. faecalis can grow on mannitol salt agar and ferment mannitol, while E. faecium lacks these phenotypes. These data reveal that we now have rapid, cost effective ways to identify enterococci to the species, and not just genus, level and have significance for patient treatment in hospitals.

关键词： enterococci Staphylococcus antibiotic resistance bacteriology microbiology

Are extrafloral nectaries efficient against herbivores? Herbivory and plant defenses in contrasting tropical species

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Journal of Plant Ecology 2020年第4期13卷 423-430页

作者： Cássio Cardoso Pereira maria Gabriela Boaventura Gislene carvalho de Castro Tatiana Cornelissen Departamento de Genética Ecologia e EvoluçãoUniversidade Federal de Minas Gerais31270-910 Belo HorizonteMGBrazil Departamento de Ciências Naturais Universidade Federal de São João del-Rei36301-160 São João del-ReiMGBrazil

Aims Plants have limited resources for defenses and species that invest in biotic defenses might exhibit leaves that invest less in other types of *** have investigated whether plants that have few mechanical defenses,but have extrafloral nectaries(EFNs)patrolled by ants,are less prone to herbivory,compared with plants without EFNs that have tougher *** Data from the literature were extracted to examine the reported levels of herbivory in plants with or without *** a savanna vegetation in southern Brazil,field data were collected in leaves from six tropical species and herbivory and specific leaf area(SLA)levels were *** further evaluated differences in herbivory and SLA among species and between plants with or without *** order to test the relationship between herbivory and leaf toughness we regressed average herbivory and average SLA per *** Findings Plants exhibited variable levels of leaf damage,but plants without ant defenses experienced the highest levels of leaf area loss to *** of mechanical defenses were also variable among the plant *** without EFNs were tougher,exhibiting lower values of *** plants without EFNs had more sclerophyllous leaves,this mechanical defense was not sufficient to impair and/or reduce herbivore feeding,suggesting that the biotic defenses performed by patrolling ants might be more effective than investment in mechanical defenses associated with leaf palatability.

关键词： ants biotic defenses herbivory leaf palatability specific leaf area

Rare earth metal as a dopant element:Cerium ion as an articulator in hexavalent chromium removal by magnetic iron oxides

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Journal of Rare Earths 2023年第10期41卷 1616-1626,I0006页

作者： Pricila maria Batista Chagas Maísa M.M.Lima Aline Aparecida Caetano Lucas Bragança carvalho Nayra maria de Abreu Piva maria Eduarda Resende Luiz Iara do Rosário Guimarães Laboratory of Environmental Catalysis and New Materials Department of ChemistryFederal University of LavrasCEP 37200-900LavrasMGBrazil Department of Viticulture&Enology University of CaliforniaDavisCA95616USA Institute of Science and Technology São Paulo State University18087-180SorocabaBrazil

This study highlights introducing the rare earth metal cerium(Ce(Ⅳ))into the structure of magnetite(Fe_(3)O_(4))to achieve enhanced adsorptive properties for the removal of chromium(Cr(VI))from an aqueous *** ratios of Ce(Ⅳ)were introduced into the iron oxide matrix,termed FeCe-5,FeCe-10,and *** numerical values correspond to the nominal content of the dopant element added to the synthesis *** solid materials were characterized for morphology and chemical structure,and N_(2)physical adsorption/desorption measurements were *** solid materials doped with Ce(IV)have a high surface area compared to Fe_(3)O_(4),and the solid material with the highest content of the dopant ion(Ce(IV))has a 4-fold greater surface *** increase in the dopant content in the iron oxide structure leads to a total chromium removal of 93.3%.Isotherms studies on the solid materials show that chromium adsorption follows the Langmuir *** adsorption capacity to Fe_(3)O_(4)is 12.59 mg/g and FeCe-10 is 22.49 mg/g at 35℃.By fitting the kinetic and isothermal models,it is found that for the Fe_(3)O_(4)and FeCe-10 materials Cr(VI)removal occurs in very different ways,attributed to the different surface areas and compositions of the oxide,with the formation of the goethite(α-FeOOH)*** FeCe-10reuse process was performed and the removal capacity the Cr(VI)is reduced after the first *** result is attributed to a strong and irreversible adsorption of Cr(VI)on the FeCe-10.

关键词： Bimetallic oxides Fe-Ce interaction Oxygen vacancies Adsorption Cr(Ⅵ) Rare earths

Hydro-wind Optimal Operation for Joint Bidding in Day-ahead Market: Storage Efficiency and Impact of Wind Forecasting Uncertainty

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Journal of Modern Power Systems and Clean Energy 2020年第1期8卷 142-149页

作者： António Cerejo Sílvio J.P.S.mariano Pedro M.S.carvalho maria R.A.Calado Technical-Scientific Unity of Electrical and Industrial Engineering Polytechnic Institute of Castelo BrancoAvenida do Empresário6000-767 Castelo BrancoPortugal Department of Electromechanical Engineering University of Beira Interior and Instituto de TelecomunicacoesCalcada Fonte do Lameiro6201-001 CovilhaPortugal Department of Electrical and Computer Engineering Instituto Superior Técnicoand INESC-IDUniversity of LisbonAv.Rovisco Pais1049-001 LisbonPortuga

Wind power production is *** imbalance between committed and delivered energy in pool markets leads to the increase of system costs,which must be incurred by defaulting producers,thereby decreasing their *** avoid this situation,wind producers can submit their bids together with hydro *** the mismatches between the predicted and supplied wind power can be used by hydro producers,turbining or pumping such differences when *** study formulates the problem of hydro-wind production optimization in operation contexts of pool *** problem is solved for a simple three-reservoir cascade case to discuss optimization *** results show a depreciation in optimal revenues from hydro power when wind forecasting is *** depreciation is caused by an asymmetry in optimal revenues from positive and negative wind power *** problem of neutralizing the effect of forecasting uncertainty is subsequently formulated and solved for the three-reservoir *** results are discussed to conclude the impacts of uncertainty on joint bidding in pool market contexts.

关键词： Optimization hydro power wind power uncertainty joint bidding pool market