检索结果-南通市图书馆

American Journal of Plant Sciences 2012年第6期3卷 753-763页

作者： Takashi Yuasa Yushi Ishibashi mari iwaya-inoue Department of Bioresource Science Faculty of Agriculture Kyushu University Fukuoka Japan.

Floral and reproductive organs of higher plants are relatively sensitive to biotic and abiotic stresses compared with the vegetative organs. Calcineurin B-like molecule (CBL) interacting protein kinase (CIPK) has appeared to be involved in acquired tolerance and acclimation under environmental stresses such as salinity, drought and chilling. Semi-quantitative RT-PCR using the vegetative and reproductive organs of tomato Micro-Tom (Solanum lycopersicum L.) at the various developmental stages indicated that SlCIPK2 was expressed specifically in the floral organ. An anti-CIPK specific antibody recognized the recombinant SlCIPK2 specifically and cross-reacted to a CIPK-related polypeptide at a significant level in flower, particularly in stamen. The flower specific CIPK was tightly associated with the microsomes. In vitro pull-down assay of the recombinant SlCIPK2 showed that SlCIPK2 interacts with SlCBLs and stress-responsive transcription factors, SlERF7, SlCBF1 and SlAREB1. The present data suggested that the flower-specific CIPK, SlCIPK2, was involved in calcium signaling in tomato via CBLs and stress tolerance possibly mediated by the stress-responsive transcription factors in stamen.

关键词： Calcium CBL CIPK Flower SnRK Tomato

The Sucrose Starvation Signal Mediates Induction of Autophagy- and Amino Acid Catabolism-Related Genes in Cowpea Seedling

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2013年第3期4卷 647-653页

作者： Ayami Kaneko Eri Noguchi Yushi Ishibashi Takashi Yuasa mari iwaya-inoue Department of Bioresource Sciences Faculty of Agriculture Kyushu University Fukuoka Japan Department of Bioresource Sciences Faculty of Agriculture Kyushu University Fukuoka Japan. Graduate School of Bioresource and Bioenvironmental Sciences Kyushu University Fukuoka Japan School of Agriculture Kyushu University Fukuoka Japan

In higher plants, autophagy is bulk degradation process in vacuole necessary for survival under nutrient-limited conditions and plays important roles in senescence, development and pathogenic response, etc. Cowpea is one of the most important legume crops in semi-aride region, which is highly tolerant to drought stress. Changes of photoassimilate status by drought stress and/or sink-source balance appeared to affect autophagy and senescence of leaf in cowpea. Accordingly, we focused on roles of sucrose signal in autophagy and amino acid recycling in cowpea. Effects of starvation stress on the expression of autophagy-related genes (ATGs) and amino acid catabolism-related genes in cowpea [Vigna unguiculata (L.) Walp] were examined by Reverse transcription-polymerase chain reaction (RT-PCR) and anti-ATG8i specific antibody. Sucrose starvation stress enhanced the expression levels of VuATG8i, VuATG8c and VuATG4 incowpea seedlings. The expressions of amino acid catabolism related genes, such as asparagine synthase (VuASN1), proline dehydrogenase1 (VuProDH) and branched chain amino acid transaminase (VuBCAT2), are also up-regulated under the sucrose starvation. In contrast, high sucrose condition suppressed autophagy and the expressions of ATGs. These results indicate that sucrose starvation stress stimulates both autophagy and amino acid catabolism by regulation of ATGs and VuBCAT2. It is conceivable that sucrose starvation stress enhances autophagy in cowpea, possibly via branched chain amino acid level regulated by the starvation-induced BCAT.

关键词： Amino acid Catabolism ATG Autophagy BCAT Cowpea Starvation Stress

Wrinkled1 (WRI1) Homologs, AP2-Type Transcription Factors Involving Master Regulation of Seed Storage Oil Synthesis in Castor Bean (Ricinus communisL.)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2013年第2期4卷 333-339页

作者： Daichi Tajima Ayami Kaneko Masatsugu Sakamoto Yumena Ito Nong Thi Hue Masayuki Miyazaki Yushi Ishibashi Takashi Yuasa mari iwaya-inoue Department of Bioresource Sciences Faculty of Agriculture Kyushu University Fu- kuoka Japan Department of Bioresource Sciences Faculty of Agriculture Kyushu University Fu- kuoka Ja-pan Fukuoka Agricultural Re- search Center Chikushino Japan Graduate School of Bioresource and Bioenvironmental Sciences Kyushu University Fukuoka Japan

Among APETALA2 (AP2)-type plant specific transcription factor family, WRINKLED1 (WRI1), has appeared to be a master gene transcriptionally regulating a set of carbon metabolism- and fatty acid synthesis (FAS)-related genes responsible for seed specific triacylglycerols (TAGs) storage in oil plants. B3 type transcription factors, such as ABI3 and FUS3, are known to be involved in seed development, such as seed storage protein synthesis and maturation. Based on the recent whole genome sequence data of castor bean (Ricinus communis L.), putative WRI1 homologs (RcWRI1, RcWRI2) specifically expressed in castor bean seed have been identified by comparing organ specific expression profiles among seed development-related transcription factors, seed storage specific genes (Ricin, RcOleosin) and a set of FAS genes including genes for sucrose synthase (RcSUS2), biotin carboxyl carrier protein (a subunit of acetyl-CoA carboxylase, RcBCCP2) and ketoacyl-acyl carrier protein synthase (RcKAS1). Immunoreactive signals with WRI1, FUS3 and ABI5-related polypeptides were also detected in seed specifically, consistent with the expression profiles of seed development-related genes. The WRI1 binding consensus sites, [CnTnG](n)(7)[CG], designated as the AW-box, were found at the promoter region of RcBCCP2 and RcKAS1. Thus, RcWRI1 possibly play a pivotal role in seed specific TAGs storage during seed development by directly activating FAS -related genes.

关键词： AP2 Castor Bean Fatty Acid Oil Seed Ricinus communis L. Transcription Factor WRI1

Hsp90 and reactive oxygen species regulate thermotolerance of rice seedlings via induction of heat shock factor A2 (OsHSFA2) and galactinol synthase 1 (OsGolS1)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Agricultural Sciences 2013年第3期4卷 154-164页

作者： Nong Thi Hue Huong Thi Tran Thuy Phan Junya Nakamura Tomoe Iwata Keisuke Harano Yushi Ishibashi Takashi Yuasa mari iwaya-inoue Department of Bioresource Sciences Faculty of Agriculture Kyushu University Fukuoka Japan. Graduate School of Bioresource and Bioenvironmental Sciences Kyushu University Fukuoka Japan Graduate School of Bioresource and Bioenvironmental Sciences Kyushu University Fukuoka Japan. School of Agriculture Kyushu University Fukuoka Japan.

Heat stress induces expression of a set of thermotolerance-related genes in plants. We focused on rice (Oryza sativa L.) homologs of the gene family that encodes galactinol synthase (OsGolS), which is closely related to the Arabidopsis thaliana galactinol synthase (AtGolS) family whose expression is induced under various stresses. OsGolS1 was highly up-regulated compare to the level of OsGolS2 in re- sponse to heat stress. Interestingly, OsGolS1 was also up-regulated by treatment with the Hsp90 inhibitor, geldanamycin (GDA). Expression profiles of OsGolS1 were correlated to those of OsHsfA2 under the GDA treatments. Treatment with GDA increased expression of OsHsfA2, but marginally increased or did not change OsHsfA1 expression. Notably, gel shift assay indicated that OsHsfA2 binds directly to OsGolS1 promoter region and that OsHsfA1 also binds to the promoter regions of OsHsfA2. Both OsHsfA2 and OsGolS1 were dramatically induced in response to heat stress. Accordingly, galactinol and raffinose contents in rice seedlings increased significantly following the induction of OsGolS1. Pre-treatment of rice seedlings with raffinose or GDA improved their thermotolerance. These results suggest that OsGolS1 plays an important role in response to heat stress, possibly via the transcription cascade of OsHsfA1-OsHsfA2 that leads to galactinol and raffinose accumulation, and that the increased content of these carbohydrates is a key response factor for rice seedlings to enhance thermotolerance.

关键词： Galactinol Synthase Geldanamycin Heat Shock Factor Heat Shock Protein Oryza sativa

A Flower Specific Calcineurin B-Like Molecule (CBL)-Interacting Protein Kinase (CIPK) Homolog in Tomato Cultivar Micro-Tom (Solanum lycopersicum L.)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Removal of estrogens by electrochemical oxidation process

American Journal of Plant Sciences 2012年第6期

作者： Takashi Yuasa Yushi Ishibashi mari iwaya-inoue

来源：评论

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2014年第6期26卷 1355-1360页

作者： Vo Huu Cong Sota iwaya Yutaka Sakakibara Graduate School of Creative Science and Engineering Waseda University

Treatments of estrogens such as Estrone （El）, Estradiol （E2） and Ethinylestradiol （EE2） were conducted using an electrolytic reactor equipped with multi-packed granular glassy carbon electrodes. Experimental results showed that El, E2 and EE2 were oxidized in the range of 0.45-0.85 V and were removed through electro-polymerization. Observed data from continuous experiments were in good agreement with calculated results by a mathematical model constructed based on mass transfer limitation. In continuous treatment of trace estrogens （1 μg/L）, 98% of El, E2 and EE2 were stably removed. At high loading rate （100 μg/L）, removal efficiency of E1 was kept around 74%-88% for 21 days, but removal efficiency reduced due to passivation of electrodes. However, removal efficiency was recovered after electrochemical regeneration of electrodes in presence of ozone. Electric energy consumption was observed in the range of 1-2 Wh/m3. From these results, we concluded that the present electrochemical process would be an alternative removal of estrogens.

关键词： electro-polymerization electrode regeneration endocrine disrupting chemicals estrogen granular reactor

Stress Dependence of Transformation Plastic Behavior

在线全文

学校读者我要写书评

暂无评论

材料热处理学报 2004年第5期25卷 208-213页

作者： Tatsuo inoue Fukuyama University Fukuyama/Himshima Japan

Transformation plasticity is known to play an important role in the course of heat treatment processes, and so affect the results of heat treatment simulations, which means that the transformation plasticity coefficient is necessary to be identifies. The authors developed a new method by use of four-point bending system of a beam to identify transformation plasticity coefficient taking advantage of its easiness and high accuracy compared with other conventional methods like tension test, etc., and identified the coefficient for four kinds of steels; plane carbon steel, Cr-steel, Cr-Mo steel and bearing steel. In this paper, further experimental data are presented for the steels if the coefficient Kp during pearlite transformation depend on the applied stress. Obtained results reveals that the stress dependence of Kp is rather trivial except for uncertain tendency detected in plane carbon steel. The relation between the Kp and carbon content included is discussed in comparison with other data referred from references.

关键词：相变塑性四点弯曲试验应力关系金属热机械性能

Synthesis,structure and mechanical properties of Zr-Cu-based bulk metallic glass composites

在线全文

学校读者我要写书评

暂无评论

International Journal of Minerals,Metallurgy and Materials 2010年第2期17卷 208-213页

作者： Akihisa inoue Institute for Materials Research Tohoku University

The unusual glass-forming ability（GFA） of the Zr48Cu36Ag8Al8 alloy and the high ductility of the Zr48Cu36Ag8Al8 metallic glass-matrix composites containing Ta powder were *** bulk metallic glass rod with a diameter of 25 mm was successfully synthesized using copper mold casting for the Zr48Cu36Ag8Al8 *** GFA of this alloy was found to be related to a large supercooled liquid region and a quaternary eutectic point with low melting *** bulk metallic glass matrix composites were prepared by introducing extra Ta particles into the Zr48Cu36Ag8Al8 *** composites consist of Ta particles homogenously distributed in the Zr48Cu36Ag8Al8 metallic glass *** optimum content of Ta powder is 10at%for the composite with the highest plasticity,which shows a plastic strain of 31%.

关键词： bulk metallic glass composites glass forming ability Zr-Cu-based alloy plastic strain

Large Eddy Simulation of Methane Non-premixed Flame Using the Laminar Flamelet Model

在线全文

学校读者我要写书评

暂无评论

Journal of Thermal Science 2011年第6期20卷 534-542页

作者： mari Mitani Yuta Ito Nobuhiko Yamasaki Department of Aeronautics and Astronautics Kyushu University

The large eddy simulation (LES) using the steady laminar flamelet model is applied to a simple turbulent jet flame with 33.2% H2,22.1% CH4 and 44.7% N2 at the Reynolds number of 15,200 in order to validate the numerical methods and to investigate the flame *** the validation,the detailed experimental data of DLR-A flame is *** numerical results are in reasonable agreement with experimental results except mass fractions of minor *** the flow field,the break-down of the potential core,the vortex structure and the mixing intensity are well *** the combustion field,mass fractions of major species (H2O,CO2,CO) are well predicted *** species are well predicted *** the present study,the simulations conducted on the Cartesian and cylindrical grids with approximately 6.6× 10 5 nodes are compared.

关键词： Cartesian grid cylindrical grid validation calculation

Estrogens and the regulation of glucose metabolism

在线全文

学校读者我要写书评

暂无评论

World Journal of Diabetes 2021年第10期12卷 1622-1654页

作者： marià Alemany Faculty of Biology University of BarcelonaBarcelona 08028CataloniaSpain

The main estrogens:estradiol,estrone,and their acyl-esters have been studied essentially related to their classical estrogenic and pharmacologic ***,their main effect in the body is probably the sustained control of core energy *** nuclear and membrane receptors show an extraordinary flexibility in the modulation of metabolic responses,and largely explain gender and age differences in energy metabolism:part of these mechanisms is already sufficiently known to justify *** regard to energy,the estrogen molecular species act essentially through four key functions:(1)Facilitation of insulin secretion and control of glucose availability;(2)Modulation of energy partition,favoring the use of lipid as the main energy substrate when more available than carbohydrates;(3)Functional protection through antioxidant mechanisms;and(4)Central effects(largely through neural modulation)on whole body energy *** the different actions of estrone,estradiol and their acyl esters,a tentative classification based on structure/effects has been *** separately or as a group,estrogens provide a comprehensive explanation that not all their quite diverse actions are related solely to specific *** a group,they constitute a powerful synergic action *** consequence,estrogens may be considered wardens of energy homeostasis.

关键词： Estrogens Insulin Estrogen receptors Energy metabolism Glucose Antioxidants Metabolic syndrome