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Molecular Identification of Mycobacterium Strains Responsible of Bovine Tuberculosis Cases in Bobo-Dioulasso Slaughterhouse, Burkina Faso

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Advances in Microbiology 2024年第2期14卷 105-114页

作者： mariétou konate Aminata Fofana^# Yacouba Kouadima Aboubacar Sidiki Ouattara Adama Sanou UFR/Sciences et Techniques Universit&eacute Catholique de l’Afrique de l’Ouest Unit&eacute Universitaire &agrave Bobo (UCAO/UUB) Bobo-Dioulasso Burkina Faso Departement Biomé dical et Sant&eacute Publique (BioMed/SP) Institut de Recherche en Sciences de la Sant&eacute (IRSS) Bobo-Dioulasso Burkina Faso Departement de Biochimie-Microbiologie UFR Sciences de la Vie et de la Terre Universit&eacute Nazi BONI Bobo-Dioulasso Burkina Faso Centre de Recherche en Sciences Biologiques Alimentaires et Nutritionnelles (CRSBAN) Universit&eacute Joseph KI-ZERBO Ouagadougou Burkina Faso Laboratoire de Mycobacté riologie Centre MURAZ Institut National de Sant&eacute Publique Bobo-Dioulasso Burkina Faso

Bovine tuberculosis (bTB) is an endemic zoonosis significantly affects animal health in Burkina Faso. The primary causative agent is Mycobacterium tuberculosis (M. tuberculosis) complex, mainly M. bovis. Cattle are considered as natural reservoir of M. bovis. However, in Burkina Faso, the circulation of these strains remains poorly understood and documented. This study aimed to identify and characterize Mycobacterium strains from suspected carcasses during routine meat inspection at Bobo-Dioulasso refrigerated slaughterhouse. A prospective cross-sectional study was conducted from January 2021 to December 2022 on cases of seizures linked to suspected bovine tuberculosis. Microbiological and molecular analyzes were used for mycobacterial strain isolation and characterization. Out of 50 samples, 24% tested positive by microscopy and 12% by culture. Molecular analysis identified 6 strains of Mycobacteria, exclusively Mycobacterium bovis specifically the subspecies bovis (Mycobacterium bovis subsp bovis). In conclusion, M. bovis subsp bovis is the primary agent responsible for bovine tuberculosis in Bobo-Dioulasso. Continuous monitoring of mycobacterial strains is therefore necessary for the effective control of this pathology in the local cattle population.

关键词： Bovine Tuberculosis Mycobacterium bovis Molecular Identification Cattle Population Burkina Faso

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Gross Alpha and Beta Activities and Related Lifetime Risks Assessment Due to Ingestion of Drinking Water from Different Sources in the District of Abidjan, Cote d’Ivoire

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World Journal of Nuclear Science and Technology 2024年第1期14卷 86-96页

作者： Ponaho Claude Kezo Issa konate Dabo Salif Ignace Agba Laboratory of Environmental Science and Technology University Jean Lorougnon Guede (UJLoG) Daloa C&#244te d’Ivoire Laboratory of Environmental and Solar Energy Sciences University Felix Houphouet Boigny (UFHB) Abidjan C&#244te d’Ivoire Laboratory of Fundamental and Applied Physics University Nangui Abrogoua (UNA) Abidjan C&#244te d’Ivoire

Drinking good quality water is essential for better health. It is therefore essential to assess the radiological quality of all water consumed in the District of Abidjan in order to prevent related hazards. Thus, the objective of this study was to assess the risk of cancer due to the ingestion of alpha and beta emitting radionuclides in the different types of water consumed in the region. A total of 63 water samples with 43 tap water samples, 5 bottled mineral water and 15 sachet water samples was collected and taken to GAEC laboratory for analysis. The low background Gas-less Automatic Alpha/Beta counting system (Canberra iMaticTM) was used to determine alpha and beta activity concentrations. Activity concentrations of both gross alpha and gross beta obtained in water sample were respectively lower than the WHO recommended limits of 0.1 Bq/l and 1 Bq/l. Also, the annual effective dose and total equivalent effective dose found in mineral bottled water samples were higher than in other types of water. The assessment of radiological lifetime risk has shown values of cancer risk due to ingestion alpha and beta emitters lower than recommended limit. These results indicate that there is no health hazard associated to consumption of water in the District of Abidjan.

关键词： Gross Alpha and Beta Activities Drinking Water Effective Dose Radiological Lifetime Risks

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Assessing the Impact of Gold Mining on the Quality of Water Resources in the Commune of Meguet, Burkina Faso

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Journal of Water Resource and Protection 2024年第4期16卷 281-292页

作者： Issoufou Ouedraogo Samuel Lankoande Yacouba konate Boukary Sawadogo Nicolas Kagambega Martin Lompo Higher School of Engineering (ESI) University of Fada N&#8217Gourma Fada N&#8217Gourma Burkina Faso Geosciences and Environment Laboratory (LaGE) Joseph KI-ZERBO University Ouagadougou Burkina Faso Water Hydro-Systems and Agriculture Laboratory (LEHSA) International Institute of Water and Environmental Engineering (2iE) Ouagadougou Burkina Faso

Despite its often illegal nature, artisanal gold mining in Burkina Faso contributes to the economic and social development of the country. However, the rudimentary techniques used in gold panning have a significant impact on the environment due to inappropriate practices and the use of various chemical substances. This study aims to assess the impact of artisanal gold mining on the quality of water resources in a rural community at Méguet, Burkina Faso. To this end, surface and groundwater samples were collected and analyzed at the BUMIGEB laboratory. Field results show that the waters are slightly alkaline (6.97 < pH < 8.1), weakly mineralized and conductive (124 < EC < 543 μS/cm), with temperatures ranging from 24.6˚C to 31.6˚C. In addition, trace metals (TMEs) analyzed from surface and subsurface waters show very high levels, generally deviating from the levels recommended by WHO guidelines for Burkina Faso. Trace metals contamination of water resources in the commune of Méguet is mainly due to Fe (3.78 - 11.12 mg/kg), Hg (0.03 - 0.29 mg/kg), As (0.01- 6.31 mg/kg) and Pb (0.01 - 3.8 mg/kg). This study can serve as a basis for guiding national environmental policies to protect the water resources of the Méguet mine.

关键词： Gold Panning Water Quality Trace Metal Méguet Burkina Faso

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Mitigating Iron Toxicity by Using Rock Phosphate to Improve Rice Productivity

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Agricultural Sciences 2024年第4期15卷 423-438页

作者： Adama Bagayogo Honoré Kam Jacques Sawadogo Moumouni konate Moussa Sie Satoshi Nakamura Fujio Nagumo Mahamadou Sawadogo Ouaga I Uviversity Jospeph Ki-Zerbo Life and Earth Sciences Training and Research Unit (UFR/SVT) Ouagadougou Burkina Faso National Center for Scientific and Technological Research National Institute of Environment and Agricultural Research (INERA) Bobo-Dioulasso Burkina Faso Japan International Research Center for Agricultural Sciences Ibaraki Japan

Iron toxicity is a major constraint to rice production, particularly in highly weathered soils of inland valleys in Sub-Saharan Africa where the rice growing area is rapidly expanding. This study aimed to improve the productiveness of iron toxicity sensitive’s rice fields as well as in the unsensitive fields by using local phosphate fertilizers. Eighteen (18) rice genotypes were been assessed in a split plot design in two areas: without iron toxicity and with iron toxicity. NPK, NK, Rock Phosphate, Triple super phosphate, Calcined phosphate and Acidulated phosphate were used as fertilizers. Data collection was focused on agronomic traits and yield (g/m2). The best fertilizers in the area without iron toxicity were NPK (820.2 g/m2) and triple super phosphate (751.7 g/m2). In the iron toxicity area, the best yields were performed by NPK (785.5 g/m2) and raw calcined phosphate (698.3 g/m2). Yet, the Accessions 15, Accessions 225, Accessions 226 and Accessions 270 were rainfed rice genotypes while CC109 A, HB 46 and HB 62 were low-land/irrigated rice genotypes. NPK, NK and acidulated phosphate fertilizers alleviate the best, iron toxicity in both sensitive and unsensitive rice fields.

关键词： Oryza NPK NK Rock Phosphate Rice

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Analysis of the Quality of Physical and Chemical Parameters of Groundwater in Three Localities in the Sub-Prefecture of Zanzra (Ivory Coast)

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Journal of Materials Science and Chemical Engineering 2024年第4期12卷 1-17页

作者： Djedjess Essoh Jules-César Meledje Bi Irié Marc Gouli Drissa Ouattara Kra Vanh Eric-Simon Zran Adjourma konate Albert Trokourey Laboratoire de Chimie des Eaux (LCE) de l’ Ecole Normale Sup&#233rieure d&#8217Abidjan Abidjan C&#244te d&#8217Ivoire Laboratoire de Constitution et Ré action de la Mati&#232re (LCRM) &#224 l&#8217UFR SSMT-Universit&#233 F&#233lix Houphou&#235t-Boigny (UFHB) de Cocody Abidjan C&#244te d&#8217Ivoire

The availability of groundwater of acceptable quality has become a difficult challenge to meet in many countries, particularly Côte d’Ivoire. The State of Côte d’Ivoire and rural populations are building hydraulic infrastructure for this purpose. However, the quality of water coming from these infrastructures remains to be determined. This study therefore aims to analyze certain physicochemical parameters of the groundwater of Gloazra, Kouezra and Trahonfla. These are temperature, pH, electrical conductivity, TDS, salinity level, hardness, alkalinity, chloride content and bicarbonate. To determine the pH, electrical conductivity, temperature, TDS, and salinity rate, an electrochemical analysis was carried out. The titrimetric analysis made it possible to determine the chloride and bicarbonate content, alkalinity, and hardness. The results indicate that the studied groundwater is soft and acidic with good mineralization. This water does not present a health hazard to consumers but can affect hydraulic equipment and household containers.

关键词： Ivory Coast Gloazra Trahonfla Kouezra Physicochemical Parameters Water WHO Standards

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Epidemiological, Clinical and Coronary Angiography Aspects of Acute Coronary Syndrome in the Cardiology Department of the Hospital Mother-Child “Le Luxembourg”Mali

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World Journal of Cardiovascular Diseases 2024年第4期14卷 228-233页

作者： Sidibé Samba toure Mamadou konate Massama Sankaré Hamma Traoré Ousmane Mahamat Guire Sadik Diall Mahan Ameri Dicko Bahaide Daffe Sanoussy Keita Asmaou Thiam Coumba Adiaratou Diarra Soumaila Menta Ichaka Cardiology Department CHU-M&#232re Enfant Bamako Mali Cardiology Department CHU-Point G Bamako Mali Faculty of Medicine University of Sciences Techniques and Technologies of Bamako Bamako Mali Cardiology Department CHU-Gabriel Tour&#233 Bamako Mali Cardiology Department Mali Hospital Bamako Mali

Introduction: Cardiac catheterisation plays a fundamental role in the management of acute coronary syndrome. These explorations require heavy, complex and costly equipment and a large team of doctors, nurses and technicians with highly specialized training. Aims: To describe epidemiological, clinical and coronary angiography aspects of patients with acute coronary syndrome. Patients and Methods: Descriptive study from September 2019 to December 2023 in the Cardiology Department of the Hôpital Mère-Enfant of Bamako. Inclusion criteria were patients admitted for coronary angiography with the diagnosis of acute coronary syndrome. Results: During the study period, 1253 patients underwent coronary angiography, 596 of whom had acute coronary syndrome as an indication, representing a hospital frequency of 47%. Sex-ratio was 2.10. Mean age of patients was 58.5 ± 11.39 years. ST elevation acute coronary syndrome was the most common indication with 63.92% of cases. High blood pressure was the main cardiovascular risk factor with 58.7% of cases, and radial access approach was used in 98% of cases. Coronary angiography was pathological in 91.70% of cases (n = 548). Patients with lesions of anterior interventricular artery were 73.73% of cases. Tritruncal lesions accounted for 40.63% of cases. Conclusion: ST elevation acute coronary syndrome is the most frequent manifestation of acute coronary syndrome. Anterior interventricular artery is most often the culprit lesion for our patients.

关键词： Coronary Angiography Acute Coronary Syndrome Bamako Mali

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Evolution of Viral Load in Patients Infected with HIV-1 at Point G University Hospital

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Open Journal of Medical Microbiology 2024年第1期14卷 66-76页

作者： A. Maiga D. Kone D. M. Coulibaly Ag M. Baraika A. Traore S. S. Diakite I. I. Maiga I. konate A. I. Maiga Faculty of Medicine and Odontostomatology of Bamako (FMOS) University of Science of Technical and Technology De Bamako Bamako Mali Laboratory of Medical Biology and Hospital Hygiene of Point-G University Hospital Bamako Mali Faculty of Pharmacy of Bamako (FaPh) University of Science of Technical and Technology De Bamako Bamako Mali Laboratory of the Luxembourg Mother-Child Hospital Bamako Mali National Institute of Public Health Bamako Mali National Institute for Training in Health Sciences Bamako Mali Laboratory of the Kati University Hospital Center Bamako Mali Infectious and Tropical Diseases Department of Point-G University Hospital Bamako Mali Laboratory of CHU Gabriel touré Bamako Mali

Introduction: HIV, the human immunodeficiency virus, is the etiological agent of acquired immunodeficiency syndrome (AIDS). The aim of this study was to assess the evolution of the viral load in patients under treatment. Methodology: This was a study carried out from July 2017 to June 2022 at the Point G University Hospital laboratory. The determination of the viral load of patients was carried out by PCR on the ABOTT M2000sp/rt platform. Results: A total of 129 patients infected with HIV-1, aged 19 to 72 years with a mean age of 40.05 years ± 10.71;all on antiretroviral chemotherapy. The female gender predominated among our patients. The most common treatment regimen was 2INTI + 1INNTI with 72.9% followed by 2INTI + 1INI with 13.2%. As for the combinations of molecules, the combination TDF + 3TC + EFV and TDF + 3TC + DTG predominated, respectively 65.1% and 13.2%. 89.9% of our patients had undetectable viremia after 12 months of treatment (p < 0.005) with an average viral load which had evolved from 681315.65 copies/ml ± 1616908.484 to M0 at 5742.36 copies /ml ± 35756.883 at M12 (p Conclusion: Generally speaking, antiretroviral treatment had contributed to controlling viral loads, however the therapeutic combination TDF + 3TC + DTG had made it possible to obtain more patients with undetectable viremia instead.

关键词： HIV-1 Treatment Viral Load Point G University Hospital

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Maternal and Perinatal Prognosis of Arterial Hypertension and Pregnancy in a Peripheral Health Center in Mali

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Open Journal of Obstetrics and Gynecology 2024年第5期14卷 855-867页

作者： Samaké Alou Diarra Lasseny Keita Mamadou Haidara Dramane Haidara Mamadou Diallo Mamadou Konaté Moussa Maiga mariam MS Ag Med Elméhdi Elansari Kassogue Djibril Dao Seydou Zana Samake Hawa konate Karim Dembele Bertin Coulibaly Moussa mariko Seydou Colette Dohinnon Reference Health Center in Commune VI of the Bamako District Bamako Mali Bamako Dermatology Hospital (HDB) Bamako Mali Kalaban Coro Reference Health Center Bamako Mali Timbuktu Hospital Timbuktu Mali Reference Health Center of Commune II of the Bamako district Bamako Mali Reference Health Center in Commune V of the Bamako district Bamako Mali National Institute for Training in Health Sciences Bamako Mali Mali Hospital Bamako Mali

Introduction: Maternal mortality constitutes a public health problem and its rate is an indicator of a country’s development. Among the causes of maternal and perinatal death, high blood pressure associated with pregnancy occupies a significant part. It represents 5% of direct maternal deaths. Objective: to study the maternal and perinatal prognosis of high blood pressure during pregnancy in the Tenenkou reference health center in Mali. Methodology: This was a descriptive, analytical and retrospective cross-sectional study over a period of twelve months from January 1, 2021 to December 31, 2021 and involving 144 cases of high blood pressure associated with pregnancy. Results: We obtained a frequency of 11.75%. The majority of patients 70.9% were aged between 20 - 35 years. The important risk factor found was young age. During our study, 46.5% of patients had performed at least one CPN and only 13.9% performed 04 CPN. Pre-eclampsia was the most common type of high blood pressure during pregnancy, i.e. 61.1%. Eclampsia and retroplacental hematoma were the most common maternal complications, respectively 27.8% and 11.1%. The most common fetal complications were premature births and fetal distress with 20.9% and 17.4% respectively. Conclusion: Hypertension associated with pregnancy still remains a major cause of maternal-fetal morbidity and mortality in our context where diagnosis is often late. The main clinical form was preeclampsia. Eclampsia and retroplacental hematoma were the most frequent maternal complications. Fetal complications were mainly prematurity and fetal distress.

关键词： Pregnancy High Blood Pressure Prognosis

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Maternal Fetal Prognosis: Pregnancy and Delivery in Women of 40 Years Old and Over in a Second Reference Hospital in Bamako

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Open Journal of Obstetrics and Gynecology 2024年第4期14卷 509-519页

作者： Samaké Alou Diarra Lasseny Keita Mamadou Haidara Dramane Haidara Mamadou Diallo Mamadou Konaté Moussa Maiga mariam Mohamed Saydi Ag Med Elmehdi Elansari Kassogue Djibril Seydou Zana Dao Samake Hawa konate Karim Dembele Bertin Coulibaly Moussa mariko Seydou Collarete Dohino Reference Health Center of Commune VI of the Bamako District Bamako Mali Bamako Dermatology Hospital (BDH) Bamako Mali Kalaban Coro Reference Health Center Bamako Mali Timbuktu Hospital Timbuktu Mali Reference Health Center of Commune II of the Bamako District Bamako Mali Reference Health Center in Commune V of the Bamako District Bamako Mali National Institute for Training in Health Sciences Bamako Mali Mali Hospital Bamako Mali

Introduction: Late pregnancies are considered a public health problem linked to maternal mortality due to their complications. These risks, which worsen with maternal age, should be better known and taken into account in monitoring these pregnancies. Objective: To evaluate the impact of age on the prevalence of obstetric and neonatal complications among parturients of 40 years old and over. Methodology: This was an 11-year retrospective analytical case control study from January 1, 2007 to December 31, 2017. The study population was female patients of 40 years old or older for cases and female patients younger than 40 years of control case. Results: In total, we collected 640 cases of parturients of 40 years old and over out of a total of 84,234 deliveries during the study period;i.e. a frequency of 0.76%. Spontaneous abortion increased in patients of 40 and over with 8.28%. Hypertension and diabetes were higher of women of 40 and over, 52.65%. Concerning the method of admission: 55% of parturients of 40 and over were referrals. Incidents occurring among women of 40 and over were more pronounced than in control cases. Complications during vaginal delivery, cervical tear, perineal tear, uterine dehiscence and uterine rupture are high in our cases, i.e. 11.88%;3.59%;2.19%;2.66. During our study, depending on the type of delivery, the cesarean rate was much higher among women of 40 and over than among control cases, i.e. 54.22% versus 12.24%. Hemorrhages in the 3rd trimester were the main indications for cesarean section. Hemorrhages in the 3rd trimester were more frequent with women of 40 and over, i.e. 10.47%;5.94% versus 1.74% and 0.42%. As for the different types of malformations in parturients, women under 40 years old fetal malformations were absent in 92.29%, on the other hand they were more frequent in patients with 40 years and over, i.e. a normal frequency of 36.72%. Conclusion: Pregnancy at a late age exposes the elderly patient and the newborn to several ri

关键词： Advanced Maternal Age Childbirth Risk Mali

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Epidemiological-Clinical Aspects of Uterine Leiomyoma at the Reference Health Center of Commune VI of the Bamako District

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Open Journal of Obstetrics and Gynecology 2024年第5期14卷 674-681页

作者： Alou Samaké Lasseny Diarra Mamadou Keita Dramane Haidara Mamadou Haidara Coulibaly Soumana Mamadou Diallo Moussa Konaté mariam Maiga MS Ag Med Elméhdi Elansari Kassogue Djibril Dao Seydou Zana Samake Hawa konate Karim Dembele Bertin Coulibaly Moussa mariko Seydou Colette Dohino Reference Health Center of Commune VI of the Bamako District Bamako Mali Bamako Dermatology Hospital (HDB) Bamako Mali Kalaban Coro Reference Health Center Bamako Mali Hangadoumbo Moulaye touré Hospital in Gao Gao Mali Timbuktu Hospital Tomboctou Mali Reference Health Center of Commune II of the Bamako District Bamako Mali Reference Health Center in Commune V of the Bamako District Bamako Mali National Institute for Training in Health Sciences Bamako Mali Mali Hospital Bamako Mali

Introduction: Uterine fibroid is a mixed mesenchymal tumor, developing from smooth muscle cells of the myometrium, separated by connective tissue. The majority of fibroids are asymptomatic and do not require any intervention or other exploratory measures. However, in some cases fibroids are symptomatic, their treatment should aim to improve symptoms and quality of life. Objectives: Describe the epidemiological-clinical aspects of uterine fibroids in the gynecology and obstetrics department of the reference health center of commune VI of the Bamako district. Methodology: This was a retrospective descriptive study carried out over a period of one year. It concerns all patients seen in consultation in the gynecology and obstetrics department of the reference health center of commune VI. Results: during the study period, the uterine fibroid frequency was 1.80%. During this same period, fibroids represented 5.59% of gyneco-obstetric pathologies operated on in the department. The 30 - 45 year old age group was the most represented with a frequency of 75.63%. The average age was 36.87 years ± 6.2 years with extremes of 25 and 63 years. Married women were the most represented 97.48%. The vast majority of our patients 95.8% were not postmenopausal. The multigravidas were the most represented, i.e. 37.50% with a large part of the pauciparous 41.29%. In our patients, 96.64% had a clinical symptom on their fibroid with the main reason for consultation being the sensation of a pelvic mass in 97.48%. We recorded three cases of infertility as associated factors. Ultrasound was performed in all patients. The location of the myxomatous nuclei was subserosal in the majority in 42.86% and with multiple nodules in 84.85% of cases. The main indication for myomectomy was the failure of medical treatment in 86.49% of cases. Surgical treatment was mainly a myomectomy 93.30%, a hysterectomy was indicated in 6.70%. No cases of death were recorded. Conclusion: Uterine fibroid is a gynecologic

关键词： Myomas Surgery Myomectomy

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