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Methylation changes at the GNAS imprinted locus in pancreatic cystic neoplasms are important for the diagnosis of malignant cysts

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World Journal of Gastrointestinal Oncology 2020年第9期12卷 1056-1064页

作者： Sandra Faias Marlene Duarte Luísa pereira Paula Chaves Marília Cravo Antonio Dias pereira Cristina albuquerque department of Gastroenterology Instituto Português de Oncologia de Lisboa Francisco GentilEPELisboa 1099-023Portugal Faculty of Health Sciences University of Beira InteriorCovilhã6200-506Portugal Unidade de Investigação em Patobiologia Molecular(UIPM) Instituto Português de Oncologia de Lisboa Francisco GentilEPELisboa 1099-023Portugal Centro de Matemática e Aplicações(CMA-UBI) Universidade da Beira InteriorCovilhã6200-506Portugal department of Pathology Instituto Português de Oncologia de Lisboa Francisco GentilEPELisboa 1099-023Portugal department of Gastroenterology Hospital BeatrizÂngeloLoures 2674-514Portugal

BACKGROUND Guanine nucleotide-binding protein,alpha stimulating(GNAS)mutations are characteristic of intraductal papillary mucinous neoplasms(IPMNs).Pancreatic ductal adenocarcinomas(PDACs)harboring GNAS mutations originate in *** is a complex imprinted locus that produces five transcripts regulated by differential methylated regions,NESP55,GNASAS,GNASXL,GNAS1A,and *** To evaluate if methylation changes in the differential methylated regions of GNAS locus contributed to malignant progression of pancreatic *** GNAS locus methylation was analyzed in archival pancreatic cyst fluid(PCF)obtained by endoscopic ultrasound with fine-needle aspiration by methylation specific–multiplex ligation dependent probe *** were normalized and analyzed using *** *** Fifty-two PCF samples obtained by endoscopic ultrasound with fine-needle aspiration and previously characterized for KRAS and GNAS mutations were *** final diagnoses were surgical(11)and clinicopathological(41),including 30 benign cysts,14 pre-malignant cyst,and eight malignant *** changes at NESP55,GNASAS,GNAS1A,and especially GNASXL were more frequent in malignant cysts,and NESP55 and GNASAS were useful for diagnosis.A combined variable defined as“GNAS locus methylation changes”was significantly associated with malignancy(6/8 malignant cysts and only 2/20 benign cysts)and improved *** in both maternally(NESP55)and paternally(GNASXL)derived promoters was found in 3/3 *** This is the first study to identify methylation changes in the GNAS locus,improving the diagnosis of malignant pancreatic cysts and suggesting a role in progression to PDAC.

关键词： Intraductal papillary mucinous neoplasms Pancreas cyst Methylation Biomarker GNAS locus Pancreatic neoplasm

Oxidative stability of green weakfish(Cynoscion virescens)by-product surimi and surimi gel enhanced with a Spondias mombin *** phenolic-rich extract during cold storage

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Food Bioscience 2022年第6期50卷 1260-1267页

作者： Thamyres César de albuquerque Sousa Edson Luís lima Silva Valquiria Cardoso da Silva Ferreira Marta Suely Madruga Fábio Anderson pereira da Silva Postgraduate Program in Agrifood Technology Center for HumanSocial and Agrarian SciencesFederal University of ParaíbaBananeirasParaíba58220-000Brazil Center for Human Social and Agrarian SciencesFederal University of ParaíbaBananeirasParaíba58220-000Brazil Postgraduate Program in Food Science and Technology Technology CenterFederal University of ParaíbaJardim Cidade UniversitáriaJoão PessoaParaíba58900-000Brazil

The aim of this investigation was to assess the effect of yellow mombin bagasse extract(YMBE),alone or combined with commercial cryoprotectants,on the quality changes during cold storage of surimi and surimi gel prepared with the cutting by-product of green weakfish(Cynoscion virescens).Four treatments were elaborated:SC(surimi control),SE(surimi with addition of 1%extract),SCP(surimi with the addition of 4%sorbitol+4%sucrose)and SECP(surimi with addition of 1%extract+4%sorbitol+4%sucrose).The addition of the extract was effective in maintaining the whiteness of the surimi and its gel throughout *** the end of 12 days of cold storage,the surimi gel prepared with SE treatment presented high whiteness compared to the control *** inclusion of 1%YMBE in isolation or combined with commercial cryoprotectants showed a positive effect on the inhibition of lipid and protein oxidation of *** conclusion,YMBE extract is a promising alternative to be used as a natural food preservative in surimi and surimi gel.

关键词： Natural antioxidant Cryoprotectants Gelification Oxidation Surimi

Effect of silymarin on biochemical indicators in patients with liver disease: systematic review with meta-analysis

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World Journal of Gastroenterology 2017年第27期23卷 5004-5017页

作者： Camila Ribeiro de Avelar Emile Miranda pereira Priscila Ribas de Farias Costa Rosangela Passos de Jesus Lucivalda pereira Magalhaes de Oliveira Postgraduation Program Federal University of Bahia departament of Science Nutrition Federal University of Bahia

To evaluate the effect of silymarin on the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma glutamyl transpeptidase (γGT) in patients with liver diseases. METHODSA systematic review with meta-analysis of ramdomized and controlled clinical trials was performed, evaluating the effects of sylimarin in patients with hepatic diseases, published by January 31, 2016. Clinical trials were sought on the basis of The Cochrane Central Register of Controlled Trials in the Cochrane library, PubMed/Medline, Scopus, Web of Science, lilacs and Clinical Trials. The trials with adult and elderly patients of both sexes, with liver Diseases who took oral silymarin supplementation, as extract or isolated, as well as Silymarin combined with other nutrients, were included. The trials should provide information about the intervention, such as dosages and detailing of the product used, besides the mean and standard deviation of serum levels of ALT, AST and γGT of the baseline and at the end of the intervention. RESULTSAn amount of 10904 publications were identified. From those, only 17 were included in the systematic review and 6 in the meta-analysis, according to the used selection criteria. In this meta-analysis, the results indicated a reduction of 0.26 IU/mL (95%CI: -0.46-0.07, P = 0.007) at the level of ALT and 0.53 IU/mL (95%CI: -0.74-0.32, P = 0.000) at the serum levels of AST after using the silymarin, both, statistically significant, but with no clinical relevance. There was no significant change in the γGT levels. Subgroup analyzes were also performed for the biochemical markers in relation to the type of intervention, whether silymarin isolated or associated with other nutrients and the time of intervention (whether ≥ 6 mo or degree of heterogeneity and low methodological quality in the carried out analysis. CONCLUSIONSilymarin minimally reduced, but w

关键词： Systematic review liver diseases Milk thistle Silymarin Meta-analysis

Phenological study of congeneric Myrcia species and Clethra scabra in wetland and drained habitats in a Montane Forest

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Journal of Forestry Research 2021年第4期32卷 1419-1427页

作者： Vinícius Londe Jaqueline Alves pereira Hildeberto Caldas de Sousa lisbon Portugal Ecology of Tropical Biomes Graduate Course Federal University of Ouro PretoOuro PretoMinas Gerais 35400-000Brazil department of Biodiversity Evolution and EnvironmentFederal University of Ouro PretoOuro PretoMinas Gerais 35400-000Brazil

Although important,phenological studies comparing congeneric species or the same species growing in different habitats are still scarce for the ***,we integrate phylogeny,ecology and biometeorology to verify whether the phenophases of congeneric species Myrcia laruotteana and Myrcia amazonica or Clethra scabra differ when their populations inhabit wetland and drained habitats and to determine what abiotic factors affect the vegetative and reproductive phenophases of these species in distinct habitat *** collected data on phenological events of 80 trees for 1 year in Itacolomi State Park,Brazil,and related them to abiotic local *** to our expectation,the phenophases of the congeneric species did not differ between habitats,but the reproductive phenophases of *** did and was greater in drained *** of *** were affected by the depth of the water table and maximum temperature in the wetland ***,precipitation,maximum temperature and relative humidity influenced Myrcia and Clethra in the drained *** differences between *** populations suggest that this species is phenotypically plastic and can present distinct phenophases depending on the habitat it *** the other hand,the congeneric Myrcia species may have similar phenophases in distinct habitats because of their shared similarities during their *** study provides a better understanding of the ecology of these species and their adaptations to different abiotic *** of this nature are important in a changing world and can inform strategies for adaptive management.

关键词： Abiotic factors Forest ecology Phenophases Soil types

Current applications of transperineal ultrasound in gastroenterology

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World Journal of Radiology 2016年第4期8卷 370-377页

作者： Andreia albuquerque Eduardo pereira Gastroenterology department Centro Hospitalar Sao Joao Gastroenterology department Hospital Amato Lusitano

Transperineal ultrasound is an inexpensive,safe and painless technique that dynamically and non-invasively evaluates the anorectal *** has multiple indications,mainly in urology,gynaecology,surgery and gastroenterology,with increased use in the last *** is performed with conventional probes,positioned directly above the anus,and may capture images of the anal canal,rectum,puborectalis muscle(posterior compartment),vagina,uterus,(central compartment),urethra and urinary bladder(anterior compartment).Evacuatory disorders and pelvic floor dysfunction,like rectoceles,enteroceles,rectoanal intussusception,pelvic floor dyssynergy can be diagnosed using this *** makes a dynamic evaluation of the interaction between pelvic viscera and pelvic floor musculature,with images obtained at rest,straining and sustained *** technique is an accurate examination for detecting,classifying and following of perianal inflammatory *** can also be used to sonographically guide drainage of deep pelvic abscesses,mainly in patients who cannot undergo conventional *** ultrasound correctly evaluates sphincters in patients with fecal incontinence,postpartum and also following surgical repair of obstetric *** are also some studies referring to its role in anal stenosis,for the measurement of the anal cushions in haemorrhoids and in chronic anal pain.

关键词： Transperineal ultrasound Inflammatory perianal disease Posterior compartment Obstructed defecation Fecal incontinence

Human osteoclast formation and resorptive function on biomineralized collagen

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Bioactive Materials 2022年第2期7卷 241-252页

作者： Daniel de Melo pereira Noel Davison Pamela Habibovic department of Instructive Biomaterials Engineering MERLN Institute for Technology-Inspired Regenerative MedicineMaastricht University6229 ERMaastrichtThe Netherlands

Biomineralized collagen composite materials pose an intriguing alternative to current synthetic bone graft substitutes by offering a biomimetic composition that closely resembles native *** hypothesize that this composite can undergo cellular resorption and remodeling similar to natural *** investigate the formation and activity of human osteoclasts cultured on biomineralized collagen and pure collagen membranes in comparison to cortical bone *** monocytes/macrophages from peripheral blood differentiate into multinucleated,tartrate-resistant alkaline phosphatase(TRAP)-positive osteoclast-like cells on all *** cells form clear actin rings on cortical bone,but not on biomineralized collagen or pure collagen *** form resorption pits in cortical bone,resulting in higher calcium ion concentration in cell culture medium;however,osteoclast resorption of biomineralized collagen and collagen membranes does not measurably *** of osteoclast enzymes-TRAP,carbonic anhydrase II(CA-II),and cathepsin-K(CTS-K)-is similar on all substrates,despite phenotypic differences in actin ring formation and *** mesh-like structure,relatively low stiffness,and lack of RGD-containing binding domains are likely the factors responsible for preventing formation of stable actin rings on and resorption of(biomineralized)collagen *** insight helps to guide further research toward the optimized design of biomineralized collagen composites as a more biomimetic bone-graft substitute.

关键词： Osteoclastogenesis Osteoclast resorption Biomineralized collagen Intrafibrillar mineral Bone graft substitute

Femtosecond laser-assisted deep anterior lamellar keratoplasty in phototherapeutic keratectomy versus the big-bubble technique in keratoconus

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International Journal of Ophthalmology(English edition) 2018年第5期11卷 807-812页

作者： Jarbas pereira de Macedo Lauro Augusto de Oliveira Flavio Hirai Luciene Barbosa de Sousa department of Ophthalmology and Visual Sciences Escola Paulista de MedicinaUniversidade Federal de S?o Paulo

AIM：To compare the functional and anatomic results of femtosecond laser（FSL）-assisted deep anterior lamellar keratoplasty（DALK） associated with phototherapeutic keratectomy（PTK） and FSL-assisted DALK performed using the big-bubble technique in ***：During the first phase of the study,an electron microscopy histopathology pilot study was conducted that included four unsuitable donor corneas divided into two groups：in FSL group,FSL lamellar cuts were performed on two corneas and in FSL＋PTK group,PTK was performed at the stromal beds of two corneas after FSL lamellar cuts were *** the second phase of the study,a randomized clinical trial was conducted that included two treatment groups of patients with keratoconus：group 1（n=14 eyes） underwent FSL-assisted DALK associated with PTK and group 2（n=12 eyes） underwent FSL-assisted DALK associated with the bigbubble *** main outcome measures were the postoperative visual acuity（VA） and optical coherence tomography（OCT） measurements,confocal microscopic findings,and contrast ***：In the pilot study,histopathology showed a more regular stromal bed in the FSL＋PTK *** the clinical trial,group 1 had significantly worse best spectaclecorrected VA and contrast sensitivity（P〈0.05 for both comparisons）.The residual stromal bed measured by OCT was significantly（P〈0.05） thicker in group *** microscopy detected opacities only at the donor-receptor interface in group ***：Patients with keratoconus treated with FSL-assisted DALK performed using the big-bubble technique fare better than treated with FSL-assisted DALK associated with PTK.

关键词： deep anterior lamellar keratoplasty phototherapeutic keratectomy big-bubble technique keratoconus

Implant surface features as key role on cell behavior

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BIOCELL 2022年第5期46卷 1151-1156页

作者： RAFAEL SCAF de MOLON MARTA MARIA ALVES pereira ERICA DORIGATTI de AVILA Federal University of Rio de Janeiro Rio de Janeiro21941-617Brazil São Paulo State University Araraquara14801-930Brazil

It has been recognized that physical and chemical properties of biomaterial surfaces mediate the quality of extracellular matrix(ECM)that may affect cell *** nature,ECM is a heterogeneous three-dimensional superstructure formed by three major components,glycosaminoglycan,glycoconjugate,and protein,that anchors cellular compartments in tissues and regulates the function and the behavior of *** in the biointerface alter the quality of ECM and morphology through cell surface receptors,which,in turn,enable it to trigger specific cell signaling and different cellular *** fact,a number of strategies have been used to improve the functionality of surfaces and direct cell behavior through precisely designed ***,we aimed to discuss,through a science-based viewpoint,the biomaterial surface features on cell behavior and analyze the impact of cell physical modification on dental implant development.

关键词： Biomaterial Cell adhesion Surface chemistry Surface topography

Molecule-based kinetic modeling by Monte Carlo methods for heavy petroleum conversion

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Science China Chemistry 2013年第11期56卷 1608-1622页

作者： de OliVEIRA Luís pereira VERSTRAETE Jan J. KOLB Max IFP Energies nouvelles Rond-point de l'échangeur de Solaize-BP 369360 SolaizeFrance Laboratoire de Chimie cole Normale Supérieure69364 Lyon Cedex 07France

A methodology for kinetic modeling of conversion processes is *** proposed approach allows to overcome the lack of molecular detail of the petroleum fractions and to simulate the reactions of the process by means of a two-step *** the first step,a synthetic mixture of molecules representing the feedstock is generated via a molecular reconstruction method,termed SR-REM molecular *** the second step,a kinetic Monte Carlo method,termed stochastic simulation algorithm(SSA),is used to simulate the effect of the conversion reactions on the mixture of *** resulting methodology is applied to the Athabasca vacuum residue *** adequate molecular representation of the vacuum residue is obtained using the SR-REM *** reaction simulations present a good agreement with the laboratory data for Athabasca vacuum residue *** addition,the proposed methodology provides the molecular detail of the vacuum residue conversion throughout the reactions simulations.

关键词： Monte-Carlo molecular reconstruction kinetic modeling stochastic simulation algorithm vacuum residue hydrocracking