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Multiomic investigation of Sugarcane mosaic virus resistance in sugarcane

The Crop Journal 2023年第6期11卷 1805-1815页

作者： Ricardo JoséGonzaga Pimenta Alexandre Hild Aono Roberto Carlos Villavicencio Burbano Marcel Fernando da Silva Ivan Antônio dos Anjos marcos guimarães de andrade landell marcos Cesar Gonçalves Luciana Rossini Pinto Anete Pereira de Souza Centre for Molecular Biology and Genetic Engineering University of CampinasCampinasBrazil Gustavo Galindo Velasco Campus Littoral Polytechnic Superior SchoolGuayaquilEcuador Advanced Centre for Technological Research in Sugarcane Agribusiness Agronomic Institute of CampinasRibeirão PretoBrazil Plant Protection Research Centre Biological InstituteSão PauloBrazil department of Plant Biology Institute of BiologyUniversity of CampinasCampinasBrazil

Sugarcane mosaic virus(SCMV)is the main etiological agent of sugarcane mosaic disease,which affects sugarcane and other grass *** the extensive characterization of quantitative trait loci controlling resistance to SCMV in maize,the genetic basis of this trait in sugarcane is largely ***,a genome-wide association study was performed and machine learning coupled with feature selection was used for genomic prediction of resistance to SCMV in a diverse sugarcane *** single-nucleotide polymorphisms(SNPs)explained up to 29.9%of the observed phenotypic variance and a 73-SNP set predicted resistance with high accuracy,precision,recall,and F1 scores(the harmonic mean of precision and recall).Both marker sets were validated in additional sugarcane genotypes,in which the SNPs explained up to 23.6%of the phenotypic variation and predicted resistance with a maximum accuracy of 69.1%.Synteny analyses suggested that the gene responsible for the majority of SCMV resistance in maize is absent in sugarcane,explaining why this major resistance source has not been identified in this ***,using sugarcane RNA-Seq data,markers associated with resistance to SCMV were annotated,and a gene coexpression network was constructed to identify the predicted biological processes involved in *** network allowed the identification of candidate resistance genes and confirmed the involvement of stress responses,photosynthesis,and the regulation of transcription and translation in resistance to *** results provide a practical marker-assisted breeding approach for sugarcane and identify target genes for future studies of SCMV resistance.

关键词： Saccharum SCMV GWAS Machine learning RNA-Seq

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Oxidized organic molecules in the tropical free troposphere over Amazonia

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National Science Review 2024年第1期11卷 174-184页

作者： Qiaozhi Zha Diego Aliaga Radovan Krejci Victoria A.Sinclair Cheng Wu Giancarlo Ciarelli Wiebke Scholz Liine Heikkinen Eva Partoll Yvette Gramlich Wei Huang Markus Leiminger Joonas Enroth Otso Per?kyl? Runlong Cai Xuemeng Chen Alkuin Maximilian Koenig Fernando Velarde Isabel Moreno Tuukka Pet?j? Paulo Artaxo Paolo Laj Armin Hansel Samara Carbone Markku Kulmala marcos andrade Douglas Worsnop Claudia Mohr Federico Bianchi Institute for Atmospheric and Earth System Research/Physics University of Helsinki Joint International Research Laboratory of Atmospheric and Earth System Sciences Nanjing University department of Environmental Science & Bolin Centre for Climate Research Stockholm University department of Chemistry and Molecular Biology University of Gothenburg Institute for Ion and Applied Physics University of Innsbruck Ionicon Analytik GmbH Laboratory for Atmospheric Physics Institute for Physics Research Universidad Mayor de San Andrés Institute of Physics University of Sao Paulo Institute for Geosciences and Environmental Research (IGE) University of Grenoble Alpes Agrarian Sciences Institute Federal University of Uberl?ndia Beijing Advanced Innovation Center for Soft Matter Science and Engineering Beijing University of Chemical Technology department of Atmospheric and Oceanic Sciences University of Maryland Aerodyne Research Inc. department of Environmental System Science ETH Zürich Switzerland and Laboratory of Atmospheric Chemistry Paul Scherrer Institute

New particle formation(NPF) in the tropical free troposphere(FT) is a globally important source of cloud condensation nuclei, affecting cloud properties and climate. Oxidized organic molecules(OOMs)produced from biogenic volatile organic compounds are believed to contribute to aerosol formation in the tropical FT, but without direct chemical observations. We performed in situ molecular-level OOMs measurements at the Bolivian station Chacaltaya at 5240 m above sea level, on the western edge of Amazonia. For the first time, we demonstrate the presence of OOMs, mainly with 4–5 carbon atoms, in both gas-phase and particle-phase(in terms of mass contribution) measurements in tropical FT air from Amazonia. These observations, combined with air mass history analyses, indicate that the observed OOMs are linked to isoprene emitted from the rainforests hundreds of kilometers away. Based on particle-phase measurements, we find that these compounds can contribute to NPF, at least the growth of newly formed nanoparticles, in the tropical FT on a continental scale. Thus, our study is a fundamental and significant step in understanding the aerosol formation process in the tropical FT.

关键词： oxidized organic molecules tropical aerosol formation Amazon free troposphere

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Fluid flow simulation of industrial fixed bed mixed-flow grain dryer using k-ω SST turbulence model

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International Journal of Agricultural and Biological Engineering 2021年第2期14卷 226-230页

作者： Anderson Rodrigo Visconcini Cid marcos Gonçalves andrade Alexandre Marconi de Souza Costa Post-graduation department of Mechanical Engineering University of MaringáAv.Colombo3790-Bloco 14-Sala 2487020-900Maringá-PRBrazil

The fluid dynamics analysis on industrial,mixed-flow grain dryers with fixed bed using computational techniques is necessary to assist the design of such equipment contributing to cost reduction in the dryer projects and agricultural drying operations that involve the production of grains in the *** study presents a Computational Fluid Dynamic(CFD)solution for air flow analysis in an industrial *** air flow at the inlet and outlet of the dryer was investigated using the k-ωSST turbulence *** dryer region with soybean was considered as a laminar porous medium flow in the permanent and isothermal regime,having for the model a simplified geometry with the tower considered as a porous medium and the air inlet and outlet as a turbulent fluid *** flow was treated with a permanent and isothermal *** flow and pressures were used according to design *** validate the k-ωSST turbulence model,the velocity profile at the dryer inlet was obtained experimentally,which presented results with good agreement between the numerical and the experimental *** model obtained satisfactory results of the computational validation of the air flow in the dryer with good convergence requiring a minimum of computational effort,being suitable for the simulation of industrial-scale dryers,as to its air flow through a tower,operating with fixed bed soybean in the steady and isothermal regime.

关键词： computational methods fixed bed porous medium soybean dryer turbulent flow

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Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni

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World Journal of Gastroenterology 2008年第38期14卷 5842-5850页

作者： Sheilla andrade Oliveira Bruno Solano Freitas Souza Carla Adriana guimares-Ferreira Elton Sá Barreto Siane Campos Souza Luiz Antonio Rodrigues Freitas Ricardo Ribeiro-dos-Santos Milena Botelho Pereira Soares Centro de Pesquisas Gonalo Moniz Fundao Oswaldo Cruz Rua Waldemar Falco 121 Brotas 40295-001 Salvador BA Brazil Centro de Pesquisas Aggeu Magalhes Fundao Oswaldo Cruz Recife Av Moraes Rego s/n Cidade Universitária Recife 50670-420 PE Brazil Centro de Pesquisas Gonalo Moniz Fundao Oswaldo Cruz Rua Waldemar Falco 121 Brotas Salvador 40295-001 BA Brazil Centro de Pesquisas Gonalo Moniz Fundao Oswaldo Cruz Rua Waldemar Falco 121 Brotas 40295-001 Salvador BA Brazil Hospital So Rafael Av So Rafael 2152 So Marcos Salvador 41256-900 BA Brazil

AIM： To investigate the potential of bone marrow mononuclear cells （BM-MCs） in the regeneration of hepatic lesions induced by Schistosoma mansoni （***） chronic infection. METHODS： Female mice chronically infected with *** were treated with BM-MCs obtained from male green fluorescent protein （GFP） transgenic mice by intravenous or intralobular injections. Control mice received injections of saline in similar conditions, Enzyme-linked immunosorbent assay （ELISA） assay for transforming growth factor-beta （TGF-β）, polymerase chain reaction （PCR） for GFP DNA, immunofluorescence and morphometric studies were performed. ResULTS： Transplanted GFP^＋ cells migrated to granuloma areas and reduced the percentage of liver fibrosis. The presence of donor-derived cells was confirmed by Fluorescence in situ hybridization （FISH） analysis for detection of cells bearing Y chromosome and by PCR analysis for detection of GFP DNA. The levels of TGF-β, a cytokine associated with fibrosis deposition, in liver fragments of mice submitted to therapy were reduced. The number of oval cells in liver sections of ***-infected mice increased 3-4 fold after transplantation. A partial recovery in albumin expression, which is decreased upon infection with ***, was found in livers of infected mice after cellular therapy. CONCLUSION： In conclusion, transplanted BMCs migrate to and reduce the damage of chronic fibrotic liver lesions caused by ***.

关键词： Schistosoma mansoni Liver injury Fibrosis Bone marrow cells Transplantation Albumin Oval cells

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Are models of volumetric production at the diametric distribution and total stand level mutually compatible?

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Journal of Forestry Research 2020年第5期31卷 1691-1698页

作者： marcos Felipe de Oliveira Valeriano Eder Pereira Miguel Pedro Guilherme de andrade Vasconcelos Mauro Eloi Nappo Humberto Angelo Alba Valeria Rezende Renan Augusto Miranda Matias Leonardo Job Biali Ilvan Medeiros Lustosa Junior department of Forestry Engineering University of Brasilia(UnB)Brası´liaBrazil Federal Institute of Brasılia(IFB) BrasıliaBrazil

The objective of this work was to compare estimates generated by a diametric distribution model and a total stand model against the pre-cut *** model efficiency was also *** were evaluated from 30 permanent sample plots in a Eucalyptus urophylla stand,comprising 24 sample plots used for model fitting,and six sample plots for *** volume of wood per hectare was estimated for different productive units(sites),using 7 years as the reference *** model adjustment quality was verified by adjustment and precision statistics:the correlation between observed and predicted variables,root mean square error percentage,graphical analysis of residual distribution,and a frequency histogram for classes of relative errors and *** the two-parameter Weibull probability density function adhered to the data for tree evolution in diameter classes for the reference age(7 years)in the different productivity classes,it generated imprecise estimates of the number of ***,it produced inaccurate volumetric production *** total stand model provided reliable projections of production volumes in different productivity classes for both adjustment types,showing compatibility with the pre-cut inventory according to a Tukey *** summary,the total stand model generated estimates that were compatible with the pre-cut inventory while the diametric distribution model did not.

关键词： Clutter model Diameter class Eucalyptus Prognosis

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Feasibility and safety of autologous bone marrow mononuclear cell transplantation in patients with advanced chronic liver disease

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World Journal of Gastroenterology 2007年第7期13卷 1067-1073页

作者： Andre Castro lyra Milena Botelho Pereira Soares luiz Flavio Maia da Silva marcos Fraga Fortes André Goyanna Pinheiro Silva Augusto César de andrade Mota Sheilla A Oliveira Eduardo lorens Braga Wilson andrade de Carvalho Bernd Genser Ricardo Ribeiro dos Santos luiz Guilherme Costa lyra Hos-pital Sao Rafael Salvador Bahia Brazil Centro de Pesquisas Goncalo Moniz Fundacao Oswaldo Cruz Salvador Bahia Brazil BGStats Consulting Graz Austria

AIM： To evaluate the safety and feasibility of bone marrow cell （BMC） transplantation in patients with chronic liver disease on the waiting list for liver transplantation. METHODS： Ten patients （eight males） with chronic liver disease were enrolled to receive infusion of autologous bone marrow-derived cells. Seven patients were classified as Child-Pugh B and three as Child-Pugh C. Baseline assessment included complete clinical and laboratory evaluation and abdominal MRI. Approximately 50 mL of bone marrow aspirate was prepared by centrifugation in a ficoll-hypaque gradient. At least of 100 millions of mononuclear-enriched BMCs were infused into the hepatic artery using the routine technique for arterial chemoembolization for liver tumors. Patients were followed up for adverse events up to 4 mo. ResULTS： The median age of the patients was 52 years （range 24-70 years）. All patients were discharged 48 h after BMC infusion. Two patients complained ofmild pain at the bone marrow needle puncture site. No other complications or specific side effects related to the procedure were observed. Bilirubin levels were lower at 1 （2.19 ± 0.9） and 4 mo （2.10 ± 1.0） after cell transplantation that baseline levels （238 ± 1.2）. Albumin levels 4 mo after BMC infusion （3.73 ± 0.5） were higher than baseline levels （3.47 ± 0.5）. International normalized ratio （INR） decreased from 1.48 （SD = 0.23） to 1.43 （SD = 0.23） one month after cell transplantation. CONCLUSION： BMC infusion into hepatic artery of patients with advanced chronic liver disease is safe and feasible. In addition, a decrease in mean serum bilirubin and INR levels and an increase in albumin levels are observed. Our data warrant further studies in order to evaluate the effect of BMC transplantation in patients with advanced chronic liver disease.

关键词： Bone marrow Cell transplantation Liverfailure Stem cell Cirrhosis

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The Engineering Professional and the Labor Market demands： Electronic Engineer and Mechanical Engineer

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Journal of Mechanics Engineering and Automation 2014年第7期4卷 616-624页

作者： Aldair Oliveira de andrade Antonio marcos de Oliveira Siqueira Lucas Henrique Figueiredo Prates Instituto de Educacao Agricultura e Ambiente Universidade Federal do Amazonas Humaita 69800-000 Brazil department of Chemistry Universidade Federal de Vicosa Vicosa 36570-900 Brazil

The purpose of this study is to present results of a jobs classified survey from a newspaper in circulation in the city of Manaus （AM, Brazil）, between late 1990s and early 2000s. The structure and functionality of the CBO （Brazilian Occupational Classification） 2002, fundamental tool for data analysis was used, followed by collection, diagnosis and data analysis to identify the characteristics and transformations of the occupational tasks of the electronic and mechanical engineer. The analysis was based on CBO data in three distinct steps described in the methodology section, compared with a study conducted in the early 1990s, and finally, the overall prevailing trend required in professional engineering training in times of complexity and in the knowledge era was presented.

关键词： Engineering education occupational profile Brazilian classification of occupations skills and abilities.

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Clique Approach for Networks:Applications for Coauthorship Networks

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Social Networking 2014年第2期3卷 80-85页

作者： marcos Grilo Rosa Inácio de Sousa Fadigas Maria Teresinha Tamanini andrade Hernane Borges de Barros Pereira Universidade estadual de Feira de Santana Feira de SantanaBrazil Instituto Federal de Educacao Ciencia e Tecnologia Simoes FilhoBrazil Universidade do estado da Bahia SalvadorBrazil Programa de Modelagem Computacional SENAI CimatecSalvadorBrazil

Coauthorship networks consist of links among groups of mutually connected authors that form a clique. Classical approaches using Social Network Analysis indices do not account for this characteristic. We propose two new cohesion indices based on a clique approach, and we redefine the network density using an index of variance of density. We have applied these indices to two coauthorship networks, one comprising researchers that published in Mathematics Education journals and the other comprising researchers from a Computational Modeling Graduate Program. A contextualized and comparative analysis was performed to show the applicability and potential of the indices for analyzing social networks data.

关键词： Clique Networks Cohesion Indices Coauthorship

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HS-SPME/GC-MS Analysis of VOC and Multivariate Techniques Applied to the Discrimination of Brazilian Varieties of Mango

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American Journal of Analytical Chemistry 2014年第3期5卷 157-164页

作者： Clícia Maria de Jesus Benevides marcos de Almeida Bezerra Pedro Afonso P. Pereira Jailson Bittencourt de andrade departamento de Ciências da Vida Universidade do Estado da Bahia (UNEB) Salvador Brazil Instituto de Química Universidade Federal da Bahia (UFBA) Salvador Brazil Laboratório de Química Analítica Universidade Estadual do Sudoeste da Bahia (UESC) Jequié Brazil

The present study analyzed the volatile compounds of three mango varieties (Tommy Atkins, Rosa and espada) using the static headspace technique with SPME coupled to CG-MS. Multivariate methodologies, such as factorial design and response surface methodology, were used to optimize the conditions of adsorption and desorption of these substances. The data were evaluated by using principal components analysis (PCA) and hierarchical grouping analysis, in order to visualize grouping tendencies of volatile compounds. Thirty-seven volatile compounds belonging to different chemical classes, such as esters, terpenes, alcohols and others, were tentatively identified in the three varieties of mango. Amongst them, twenty-three presented chromatographic peaks with relative areas larger than 2%. The multivariate analysis made it possible to visualize the grouping tendencies of the mango samples, according to the presence of their respective volatile substances, and enabled the identification of the groups of substances responsible for the discrimination among the three varieties.

关键词： Mango Volatiles Multivariate Techniques HS-SPME/GC-MS Chemometrics

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Use of Orbital Images and Interpolation Techniques: Climate Monitoring in Permanent Preservation Area (Humid Subtropical Climate)

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Journal of Geographic Information System 2020年第6期12卷 560-578页

作者： Aparecido Ribeiro de andrade marcos Aurelio Pelegrina Juliane Bereze João Anésio Bednarz Bruno Henrique Costa Toledo department of the Geography Climatology and Environmental Analysis Laboratory Universidade Estadual do Centro-Oeste Unicentro LACLIAM Guarapuava Paraná Brazil department of the Geography Geoprocessing Laboratory LABGEO Universidade Estadual do Centro-Oeste Unicentro Guarapuava Paraná Brazil

The present work sought to demonstrate the importance using geoprocessing techniques and methodologies in the identification and analysis of the temporal and spatial distribution of air temperature. Therefore, an environment with difficulties for systematic monitoring of climatic elements was selected in the Serra da esperançaárea, southern region of the State of Paraná. Two methodologies established in geoprocessing were used: the first deals with the interpretation of orbital images and the second makes reference to the interpolation of surface data. These methodologies, used in an integrated way, made it possible to know the relationship between the spatial and temporal distribution of air temperature with vegetation and the terrain altimetry. The air temperature data were monitored at 4 (four) points, following the contours of the federal highway that passes through the study area. Satellite images were obtained of July 26th, 2015 and January 18th, 2016, representing winter and summer southern conditions. The results indicated that this relationship exists and that in the absence of absolute data monitored at conventional collection points, geostatistics techniques allow good estimation. Moreover, this methodology can be used to control land use change in permanent preservation areas.

关键词： Air Temperature Topoclimate Geostatistics

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