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Low crude protein formulation with supplemental amino acids for its impacts on intestinal health and growth performance of growing-finishing pigs

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1590-1606页

作者： marcos elias duart. Wanpuech Parnsen Shihai Zhang Marvio L.T.Abreu Sung Woo Kim Department of Animal Science North Carolina State UniversityRaleighNC 27695USA

Background Low crude protein(CP)formulations with supplemental amino acids(AA)are used to enhance intestinal health,reduce costs,minimize environmental impact,and maintain growth performance of ***,extensive reduction of dietary CP can compromise growth performance due to limited synthesis of non-essential AA and limited availability of bioactive compounds from protein supplements even when AA requirements are ***,implementing a low CP formulation can increase the net energy(NE)content in feeds causing excessive fat *** supplementation of functional AA,coupled with low CP formulation could further enhance intestinal health and glucose metabolism,improving nitrogen utilization,and growth *** experiments were conducted to evaluate the effects of low CP formulations with supplemental AA on the intestinal health and growth performance of growing-finishing *** In Exp.1,90 pigs(19.7±1.1 kg,45 barrows and 45 gilts)were assigned to 3 treatments:CON(18.0%CP,supplementing Lys,Met,and Thr),LCP(16.0%CP,supplementing Lys,Met,Thr,Trp,and Val),and LCPT(16.1%CP,LCP+0.05%SID Trp).In Exp.2,72 pigs(34.2±4.2 kg BW)were assigned to 3 treatments:CON(17.7%CP,meeting the requirements of Lys,Met,Thr,and Trp);LCP(15.0%CP,meeting Lys,Thr,Trp,Met,Val,Ile,and Phe);and VLCP(12.8%CP,meeting Lys,Thr,Trp,Met,Val,Ile,Phe,His,and Leu).In Exp.3,72 pigs(54.1±5.9 kg BW)were assigned to 3 treatments and fed experimental diets for 3 phases(grower 2,finishing 1,and finishing 2).Treatments were CON(18.0%,13.8%,12.7%CP for 3 phases;meeting Lys,Met,Thr,and Trp);LCP(13.5%,11.4%,10.4%CP for 3 phases;meeting Lys,Thr,Trp,Met,Val,Ile,and Phe);and LCPG(14.1%,12.8%,11.1%CP for 3 phases;LCP+Glu to match SID Glu with CON).All diets had 2.6 Mcal/kg *** In Exp.1,overall,the growth performance did not differ among *** LCPT increased(P<0.05)Claudin-1 expression in the duodenum and *** LCP and LCPT increased(P<0.05)CAT-1,4F2hc,and B0AT exp

关键词： Amino acids Growing-finishing pigs Growth performance Intestinal health Low crude protein formulation Net energy

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Intestinal microbiota and its interaction to intestinal health in nursery pigs

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Animal Nutrition 2022年第1期8卷 169-184页

作者： marcos elias duart. Sung Woo Kim Department of Animal Science North Carolina State UniversityRaleighNC27695United States

The intestinal microbiota has gained increased attention from researchers within the swine industry due to its role in promoting intestinal maturation,immune system modulation,and consequently the enhancement of the health and growth performance of the *** review aimed to provide updated scientific information on the interaction among intestinal microbiota,dietary components,and intestinal health of *** small intestine is a key site to evaluate the interaction of the microbiota,diet,and host because it is the main site for digestion and absorption of nutrients and plays an important role within the immune *** diet and its associated components such as feed additives are the main factors affecting the microbial composition and is central in stimulating a beneficial population of *** microbiotaehost interaction modulates the immune system,and,concurrently,the immune system helps to modulate the microbiota *** direct interaction between the microbiota and the host is an indication that the mucosa-associated microbiota can be more effective in evaluating its effect on health *** was demonstrated that the mucosa-associated microbiota should be evaluated when analyzing the interaction among diets,microbiota,and *** addition,supplementation of feed additives aimed to promote the intestinal health of pigs should consider their roles in the modulation of mucosa-associated microbiota as biomarkers to predict the response of growth performance to dietary interventions.

关键词： Intestinal health Mucosa-associated microbiota Nursery pig

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Efficacy of soy protein concentrate replacing animal protein supplements in mucosa-associated microbiota, intestinal health, and growth performance of nursery pigs

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Animal Nutrition 2023年第3期14卷 235-248页

作者： Zixiao Deng marcos elias duart. Sung Woo Kim Department of Animal Science North Carolina State UniversityRaleighNC 27695USA

This study investigated the effects of using soy protein concentrate (SPC) to replace animal protein supplements on mucosa-associated microbiota,intestinal health,and growth performance of nursery ***-six newly weaned pigs (BW=6.4±0.6 kg) were allotted to 5 treatments in a randomized complete block *** were fed for 35 d in 3 phases (P;1,2,3) for 10,12,13 d,*** treatments were:(1) basal diet with fish meal (P1:4%,P2:2%,and P3:1%),poultry meal (P1:10%,P2:8%,and P3:4%),and blood plasma (P1:4%,P2:2%,and P3:1%),where SPC replacing none (NC);(2) basal diet with SPC replacing fish meal (RFM);(3) basal diet with SPC replacing poultry meal (RPM);(4) basal diet with SPC replacing blood plasma (RBP);and (5) basal diet with SPC replacing all animal protein supplements (PC).Growth performance was recorded for each *** were euthanized on d 35 to collect jejunal mucosa and tissue to evaluate intestinal health and microbiota,and ileal digesta to measure apparent ileal digestibility (AID) of *** were analyzed using the MIXED procedure of ***,RFM,RPM,and RBP did not affect growth performance,whereas PC decreased (P<0.05) ADG and *** RPM increased (P<0.05) Prevotella stercorea and decreased (P<0.05) Helicobacter *** PC decreased (P<0.05) ***,whilst increasing (P<0.05) Prevotella copri,Propionibacterium acnes,and Pelomonas *** RFM tended to increase (P=0.096) immunoglobulin A in the *** PC tended to decrease (P=0.078) jejunal crypt cell *** were no differences in the villus height,AID of nutrients,intestinal inflammation,and intestinal oxidative stress among *** conclusion,SPC can replace fish meal,poultry meal,or blood plasma individually without affecting growth performance and intestinal health,and AID of nutrients of nursery *** SPC replacing poultry meal benefitted intestinal health by reducing *** and increasing ***,SPC replac

关键词： Blood plasma Fish meal Intestinal health Nursery pigs Poultry meal Soy protein concentrate

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Comparative effects of soy protein concentrate,enzyme‑treated soybean meal,and fermented soybean meal replacing animal protein supplements in feeds on growth performance and intestinal health of nursery pigs

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畜牧与生物技术杂志:英文版 2023年第5期14卷 2033-2047页

作者： Zixiao Deng marcos elias duart. So Young Kim Yunil Hwang Sung Woo Kim Department of Animal Science North Carolina State University116 Polk HallCampus Box 7621RaleighNC 27695USA CJ Cheiljedang Seoul 04560Korea

Background Soy protein supplements,with high crude protein and less antinutritional factors,are produced from soybean meal by different *** study evaluated the comparative effects of various soy protein supplements replacing animal protein supplements in feeds on the intestinal immune status,intestinal oxidative stress,mucosaassociated microbiota,and growth performance of nursery *** Sixty nursery pigs(6.6±0.5 kg BW)were allotted to five treatments in a randomized complete block design with initial BW and sex as *** were fed for 39 d in 3 phases(P1,P2,and P3).Treatments were:Control(CON),basal diet with fish meal 4%,2%,and 1%,poultry meal 10%,8%,and 4%,and blood plasma 4%,2%,and 1%for P1,P2,and P3,respectively;basal diet with soy protein concentrate(SPC),enzyme-treated soybean meal(ESB),fermented soybean meal with Lactobacillus(FSBL),and fermented soybean meal with Bacillus(FSBB),replacing 1/3,2/3,and 3/3 of animal protein supplements for P1,P2,and P3,*** were analyzed using the MIXED procedure in SAS *** The SPC did not affect the BW,ADG,and G:F,whereas it tended to reduce(P=0.094)the ADFI and tended to increase(P=0.091)crypt cell *** ESM did not affect BW,ADG,ADFI,and G:F,whereas tended to decrease(P=0.098)protein carbonyl in jejunal *** FSBL decreased(P<0.05)BW and ADG,increased(P<0.05)TNF-α,and Klebsiella and tended to increase MDA(P=0.065)and IgG(P=0.089)in jejunal *** FSBB tended to increase(P=0.073)TNF-α,increased(P<0.05)Clostridium and decreased(P<0.05)Achromobacter and alpha diversity of microbiota in jejunal *** Soy protein concentrate,enzyme-treated soybean meal,and fermented soybean meal with Bacillus could reduce the use of animal protein supplements up to 33%until 7 kg body weight,up to 67%from 7 to 11 kg body weight,and entirely from 11 kg body weight without affecting the intestinal health and the growth performance of nursery *** soybean meal with

关键词： Enzyme-treated soybean meal Fermented soybean meal Intestinal health Nursery pigs Soy protein concentrate

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Impacts of weaning age on dietary needs of whey permeate for pigs at 7 to 11 kg body weight

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Journal of Animal Science and Biotechnology 2022年第2期13卷 593-600页

作者： Ki Beom Jang marcos elias duart. Jerry M.Purvis Sung Woo Kim Department of Animal Science North Carolina State UniversityRaleighNC 27695USA N.G.Purvis Farm Inc RobbinsNC 27325USA.

Background:Whey permeate is an effective lactose source for nursery pigs and the most benefits are obtained when pigs are at 7 to 11 kg *** weaning ages could cause different length of early-weaner phases until 7 kg BW and thus it would influence the dietary need of whey permeate during 7 to 11 kg BW of *** study aimed to evaluate if weaning ages would affect the dietary needs of whey permeate for optimum growth performance of pigs at 7 to 11 kg ***:A total of 1,632 pigs were weaned at d 21(d 21.2±1.3)or d 25(d 24.6±1.1)after *** pigs had a common early-weaner feeds until they reached 7 kg *** pigs reached 7 kg BW within a weaning age group,they were allotted in a randomized complete block design(2×4 factorial).Two factors were weaning age groups(21 and 25 d of age)and varying whey permeate levels(7.50%,11.25%,15.00%,and 18.75%).Data were analyzed using the GLM and NLIN procedures of SAS for slope-ratio and broken-line analyses to determine the growth response to whey permeate and optimal daily whey permeate intake for the growth of the pigs weaned at different ***:Pigs weaned at 21 d of age had a common diet for 11 d to reach 7 kg BW whereas pigs weaned at 25 d of age needed 2 *** G:F of pigs weaned at 25 d of age responded to increased daily whey permeate intake greater(P<0.05)than pigs weaned at 21 d of *** were obtained(P<0.05)at 88 and 60 g/d daily whey permeate intake or 17.0%and 14.4%of whey permeate for G:F of pigs weaned at 21 and 25 d of age,***:Pigs weaned at an older age with a short early-weaner phase had a greater growth response to whey permeate intake compared with pigs weaned at a younger age with a long early-weaner *** weaning ages affected dietary needs of whey permeate for optimum growth performance of pigs from 7 to 11 kg BW.

关键词： Growth performance Nursery pigs Weaning age Whey permeate

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Random noise stimulation in the treatment of patients with neurological disorders

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Neural Regeneration Research 2022年第12期17卷 2557-2562页

作者： Mateo A.Herrera-Murillo Mario Treviño elias Manjarrez Facultad de Medicina Plan de Estudios Combinados en Medicina(PECEM)UNAMCircuito InteriorCiudad UniversitariaAv.UniversidadCiudad de MéxicoMéxico Instituto de Neurociencias Universidad de GuadalajaraGuadalajaraJaliscoMéxico Instituto de Fisiología Benemérita Universidad Autónoma de PueblaPueblaMéxico

Random noise stimulation technique involves applying any form of energy(for instance,light,mechanical,electrical,sound)with unpredictable intensities through time to the brain or sensory receptors to enhance sensory,motor,or cognitive *** noise stimulation initially employed mechanical noise in auditory and cutaneous stimuli,but electrical energies applied to the brain or the skin are becoming more frequent,with a series of clinical ***,recent evidence shows that transcranial random noise stimulation can increase corticospinal excitability,improve cognitive/motor performance,and produce beneficial aftereffects at the behavioral and psychological ***,we present a narrative review about the potential uses of random noise stimulation to treat neurological disorders,including attention deficit hyperactivity disorder,schizophrenia,amblyopia,myopia,tinnitus,multiple sclerosis,post-stroke,vestibular-postural disorders,and sensitivity *** of the reviewed studies reveal that the optimal way to deliver random noise stimulation-based therapies is with the concomitant use of neurological and neuropsychological assessments to validate the beneficial *** addition,we highlight the requirement of more randomized controlled trials and more physiological studies of random noise stimulation to discover another optimal way to perform the random noise stimulation interventions.

关键词： auditory noise mechanical noise neurological disorders neuronal noise noise galvanic vestibular stimulation non-invasive brain stimulation transcranial electrical stimulation transcranial random noise stimulation

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Ecological Costs Applied in the Bleaching of Cotton： A Study for an Optimized Process

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Journal of Chemistry and Chemical Engineering 2013年第7期7卷 633-636页

作者： Rosa Jorge marcos Tambourgi elias Basile Santana Jose Carlo Curvelo Carmo Renee Simoes Argolo Arafjo Mauricio de Campos College of Chemistry Engineering State University of Campinas Sao Paulo 03008-020 Brazil Post Graduation Department College of Technology Antoine Skaf Sao Paulo 03008-202 Brazil Production Engineering Post Graduation Program Nine o f July University Sao Paulo 5001-100 Brazil Textile and Fashion Post Graduation Program Sao Paulo University S6o Paulo 03828-000 Brazil

The aim of this paper was to study three variables in hydrogen peroxide bleaching of cotton fabrics： the amount of hydrogen peroxide （H2O2）, peroxide stabilizer （Na2SiO3） and the process temperature. The factorial planning of two levels and three factors was done in order to obtain an optimized recipe for subsequent comparison with commons recipes used in industry. Were evaluated the brightness （DL）, energy consumption （dQ）, H2O2 residual （Rox）, processing time and ecological costs.

关键词： Cotton bleaching factorial planning ecological costs.

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Determination of the Stabilization Time of the Solution-Air Interface for Aggregates Formed by NaC in Mixtures with SDS and PEO, Investigated by Dynamic Surface Tension Measurements

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Soft 2014年第1期3卷 1-10页

作者： Grasiele Raupp Arlindo C. Felippe Tiago elias Allievi Frizon Luciano da Silva marcos Marques da Silva Paula Alexandre G. Dal-Bó Universidade do Extremo Sul Catarinense (UNESC) Criciúma Brazil Universidade Federal da Fronteira Sul (UFFS) Chapecó Brazil

The properties of mixtures of poly (ethylene oxide) (PEO) and mixed micelles formed from sodium cholate (NaC) and sodium dodecyl sulfate (SDS) in tris/HCl buffered solutions at pH 9.00 were investigated by measuring the mean surface tension. The variation in the superficial tension as a function of the time after formation of solutions containing PEO and NaC was characterized by monitoring the time required for the system to reach equilibrium between the micellar and aqueous phases. These results could serve as a reference for the minimum aging time required for solutions before any surface tension measurements can be performed.

关键词： Poly (Ethylene Oxide) Micelles Sodium Cholate Sodium Dodecyl Sulfate

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Assessment of the Total Organic Carbon and Conductivity in Consecutive Dyeings with Reactive Dyestuffs Using Treated Effluent by UV/H202 Photocatalysis

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Journal of Chemistry and Chemical Engineering 2014年第2期8卷 139-144页

作者： Leite Adilson da SilvaI Barco Luiz Trindade Nelson Barros Araujo Mauricio de Campos Rosa Jorge marcos Tambourgi elias Basile Santana José Carlos Curvelo Chemical Textile Post Graduation Department College of Technology SENAI Antoine Skaf $6o Paulo 03008-020 Brazil Textile and Fashion Post Graduation Program S6o Paulo University S6o Paulo 03828-000 Brazil College of Chemical Engineering State University of Campinas Campinas 13083-852 Brazil Production Engineering Post Graduation Program Nove de Julho University Sao Paulo 05001-100 Brazil

The paper was monitored the conductivity （g.L＂l of NaCI）, the absorbances values （Abs） and the amount of TOC （total organic carbon）, before and after five consecutive dyeings made with reuse water, obtained from treated effluent by UV/H202 photocatalysis. All rates of decolorization were above 92%, the removal of total organic carbon was above 89% in all treatments and the economy of NaCI was from 3.10 in the first recipe, 3.17 in the second, 3.49 in the third, 3.79 in the fourth and 4.05 in the fifth recipe. The five dyeings compared with same dyeings made with deionized water presented a color deviation （AE＊） below 1. The conventional dyeings would consume 80 L/kg against 40 L/kg used in the dyeing process proposed in this study, representing a great economy of water, with no discharge of effluents.

关键词： Conductivity reactive dyestuffs reuse of water photocatalysis textile effluent.

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Relationship of Density of Specific Prostate Antigen (DPSA) with Prostate Histopathology: A Comparative Study of Patients Addressed in a School Hospital

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Journal of Biosciences and Medicines 2017年第3期5卷 105-111页

作者： marcos Benedito Figueiredo Brandã o Maria Benedita Figueiredo Brandã o elias Sobreira Sathler Richard Raphael Borges Tavares Vieira Carolina Cadinelli Vieira Pedro Kozlowsky de Alencar Marcus Vinicius de Mello Pinto Lamara Laguardia Valente Rocha Lina Claudia Pereira Lopes Daniel Almeida da Costa Carlos Henrique Diniz Branco Luiz Gioseffi Jannuzzi School Hospital of the Dom Andre Arcoverde Foundation Valen&ccedil a Brazil Universidade Está cio de Sá Rio de Janeiro Brazil The Medical School at the Center of Higher Education of Valenç a Valen&ccedil a Brazil The Cellurare Institute Center of Laser Treatment and Cell Therapy Petr&oacute polis Brazil The Medical School at the Health Sciences Institute of the University Center of Caratinga Caratinga Brazil

Background: The prostate is the site of problems that have great clinical relevance. Beside this, the alterations in their volume can be divided into benign or malignant pathologies, but of multifactorial cause and there is no examination that alone is reliable for the diagnosis. Despite the evolution of the various methods of diagnostic imaging in the evaluation of pelvic diseases, the diagnosis of prostate cancer still requires histological confirmation obtained by transrectal ultrasound guided biopsy, a procedure that is generally safe and well tolerated by the patients. The most common histological type found in prostate biopsies is adenocarcinoma. Methodology: The objective of this study was to study the values of density of the prostate-specific antigen (DPSA) compared to histopathological results of prostate biopsy, patients attended at the Urology Outpatient Clinic of the Luiz Gioseffi Jannuzzi School Hospital from 1999 to 2007, using Exploratory, retrospective and documentary descriptive study. The sample was divided into groups of patients with adenocarcinoma and patients without adenocarcinoma, who presented only benign prostatic hyperplasia. Results: Of the 251 patients undergoing prostate biopsy, 124 were diagnosed with adenocarcinoma and 127 without adenocarcinoma. It was observed in this study that the DPSA was higher in adenocarcinoma than in the cases of patients with prostatic hyperplasia alone, and could therefore be used as an auxiliary tool in both diagnosis and follow-up of patients with adenocarcinoma. Conclusion: In this study, it can be observed that DPSA was higher in patients presenting with adenocarcinoma than in patients presenting only Benign Prostatic Hyperplasia (BPH), reinforcing its promising role to assist in the diagnosis and follow-up of patients with adenocarcinoma.

关键词： Prostate Cancer Adenocarcinoma Benign Prostatic Hyperplasia Prostate Biopsy

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