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Molecular investigation of Tuscan sweet cherries sampled over three years: gene expression analysis coupled to metabolomics and proteomics

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Horticulture Research 2021年第1期8卷 1-24页

作者： Roberto Berni Sophie Charton Sebastien Planchon Sylvain Legay marco romi Claudio Cantini Giampiero Cai Jean-Francois Hausman Jenny Renaut Gea Guerriero Department of Life Sciences University of Sienavia P.A.Mattioli 4I-53100 SienaItaly TERRA Teaching and Research Center Gembloux Agro-Bio TechUniversity of Liège5030 GemblouxBelgium Environmental Research and Innovation Department Luxembourg Institute of Science and Technology41Rue du BrillL-4422 BelvauxLuxembourg Environmental Research and Innovation Department Luxembourg Institute of Science and Technology5rue BommelL-4940 HautcharageLuxembourg Istituto per la BioEconomia(IBE CNR) Dipartimento di Scienze BioAgroAlimentarivia Aurelia 4958022 FollonicaItaly

Sweet cherry(Prunus avium L.)is a stone fruit widely consumed and appreciated for its organoleptic properties,as well as its nutraceutical *** here investigated the characteristics of six non-commercial Tuscan varieties of sweet cherry maintained at the Regional Germplasm Bank of the CNR-IBE in Follonica(Italy)and sampled ca.60 days post-anthesis over three consecutive years(2016-2017-2018).We adopted an approach merging genotyping and targeted gene expression profiling with *** complement the data,a study of the soluble proteomes was also performed on two varieties showing the highest content of *** identified the presence of flavanols and proanthocyanidins in highest abundance in the varieties Morellona and Crognola,while gene expression revealed that some differences were present in genes involved in the phenylpropanoid pathway during the 3 years and among the ***,proteomics on Morellona and Crognola showed variations in proteins involved in stress response,primary metabolism and cell wall *** the best of our knowledge,this is the first multi-pronged study focused on Tuscan sweet cherry varieties providing insights into the differential abundance of genes,proteins and metabolites.

关键词： metabolism maintained sweet

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