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Challenges in the chemotherapy of Chagas disease: Looking for possibilities related to the differences and similarities between the parasite and host

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World Journal of Biological Chemistry 2017年第1期8卷 57-80页

作者： Vitor Sueth-Santiago Debora Decote-Ricardo Alexandre Morrot Celio Geraldo freire-de-lima marco edilson freire lima Instituto Federal de Educacao Ciência e Tecnologia do Rio de JaneiroCampus Sao Goncalo Universidade Federal Rural do Rio de Janeiro Instituto de Ciências ExatasDepartamento de Química Universidade Federal Rural do Rio de Janeiro Instituto de VeterináriaDepartamento de Microbiologia e Imunologia Veterinária Universidade Federal do Rio de Janeiro

Almost 110 years after the first studies by Dr. Carlos Chagas describing an infectious disease that was named for him, Chagas disease remains a neglected illness and a death sentence for infected people in poor countries. This short review highlights the enormous need for new studies aimed at the development of novel and more specific drugs to treat chagasic patients. The primary tool for facing this challenge is deep knowledge about the similarities and differences between the parasite and its human host.

关键词： Trypanosoma cruzi Trans-sialidase Trypanothione reductase CYP51 Cruzipain Tubulin

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Phages and Nanotechnology:New Insights against Multidrug-Resistant Bacteria

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BioDesign Research 2023年第1期5卷 12-24页

作者： marco Pardo-freire Pilar Domingo-Calap Institute for Integrative Systems Biology I2SysBioUniversitat de València-CSIC46980 PaternaSpain.

Bacterial infections are a major threat to the human healthcare system worldwide,as antibiotics are becoming less effective due to the emergence of multidrug-resistant ***,there is a need to explore nontraditional antimicrobial alternatives to support rapid interventions and combat the spread of pathogenic *** nonantibiotic approaches are being developed,many of them at the interface of physics,nanotechnology,and *** physical factors(e.g.,pressure,temperature,and ultraviolet light)are typically used in the sterilization process,nanoparticles and phages(bacterial viruses)are also applied to combat pathogenic ***,phage-based therapies are rising due to the unparalleled specificity and high bactericidal activity of *** the success of phages mostly as compassionate use in clinical cases,some drawbacks need to be addressed,mainly related to their stability,bioavailability,and systemic *** phages with nanoparticles can improve their performance in ***,the combination of nanotechnology and phages might provide tools for the rapid and accurate detection of bacteria in biological samples(diagnosis and typing),and the development of antimicrobials that combine the selectivity of phages with the efficacy of targeted therapy,such as photothermal ablation or photodynamic *** this review,we aim to provide an overview of how phage-based nanotechnology represents a step forward in the fight against multidrug-resistant bacteria.

关键词： diagnosis mostly ultraviolet

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Evaluation of Physicochemical,Microbiological and Sensory Stability of Frozen Stored Vacuum-Packed Lamb Meat

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Journal of Integrative Agriculture 2013年第11期12卷 1946-1952页

作者： Rafaella de Paula Paseto Fernandes Maria Teresa de Alvarenga freire Celso da Costa Carrer marco Antonio Trindade Department of Food Engeneering College of Animal Science and Food Engineering of UniversitySo Paulo(FZEA/USP)Avenida Duque of Caxias Norte225Jardim ElitePirassunungaSo Paulo 13.635-900Brazil Department of Food Engineering FZEA/USPPirassunungaSo Paulo 13.635-900Brazil Department of Animal Science FZEA/USPPirassunungaSo Paulo 13.635-900Brazil

Nowadays, lamb meat represents only 7% of all meat produced in the world. In recent years the demand for standardized lamb meat cuts has been considered of great importance and the marketing occurs predominantly in the form of frozen cuts. Herewith, the main of this work was to evaluate the stability and safety of lamb meat during frozen storage. Meats were vacuum packed in high barrier multilayer plastic iflms and stored during 12 mon at （-18±1）℃. The meat stability was assessed by physical and chemical （lipid oxidation, objective color, pH value, cooking losses and instrumental texture）, microbiological （total count of psychrotrophic, coliform count at 45℃, coagulase-positive staphylococci and the presence of Salmonella） and sensory analysis （acceptance test and visual evaluation）. The vacuum packed lamb meat remained stable as to most physical and chemical indexes. Microbiological indexes showed good stability throughout the storage period according to Brazilian legislation standards to pathogenic microorganisms. Although a signiifcant reduction in tenderness （shear force increase from 3 to 8 kg）, it showed a good sensorial acceptance for all attributes tested, including texture, with scores of around 7 （like moderately） during the 12 mon of storage. Therefore, it can be concluded that, under the conditions applied in this study, lamb meat presents a shelf life of at least 12 mon when stored at-18℃.

关键词： acceptance test Longissimus dorsi shelf life

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Development of a Ready-to-Use PSMA-11 Kit Formulation and Biological Evaluation of Binding Affinity

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Advances in Molecular Imaging 2024年第2期13卷 7-22页

作者： Maria Renata Valente Brandão freire Natália Marchesan Bexiga marco Antonio Stephano João Ezequiel Oliveira Carlos Roberto Jorge Soares Ana Cláudia Camargo Miranda Anna Carolina de Alcântara Barbosa Marycel Figols de Barboza Laura Fernanda Garcia Luiz Felipe S. Teixeira Maria Helena Marumo Bellini Natanael Gomes da Silva Luiza Mascarenhas Balieiro Joel Mendes Elaine Bortoleti de Araújo Centro de Radiofarmcia Instituto de Pesquisas Energticas e Nucleares (IPEN) Cidade Universitria So Paulo Brasil Departamento de Tecnologia Farmacutica Faculdade de Cincias Farmacuticas (USP) Cidade Universitria So Paulo Brasil Centro de Biotecnologia Instituto de Pesquisas Energticas e Nucleares (IPEN) Cidade Universitria So Paulo Brasil Departamento de Medicina Nuclear do Hospital Israelita Albert Einstein So Paulo/SP Brasil

Introduction: 68Ga-PSMA-11 is considered the gold standard in detection of micro and oligometastases in advanced prostate cancer, being used for therapeutic planning, as well as, potentially, for evaluating response to treatment. The development of ready-to-use lyophilized kit of PSMA-11 adds quality and safety to the routine use of this radiopharmaceutical and represents a pharmacotechnical challenge as it must preserve the integrity and specificity of the ligand. Methods: PSMA-11 kit formulation was proposed, considering radiolabeling parameters and the preservation of the peptide during the lyophilization process, using mannitol as an excipient. Critical temperature characterization studies were carried out using DSC equipment and the freeze-drying process was developed. The direct radiolabeling conditions were evaluated and standardized using 68Ge/68Ga generator eluate from two different manufacturers (ITG and Eckert & Ziegler). The radiochemical purity was evaluated by TLC and HPLC. Biological evaluation was carried out with lyophilized PSMA-11 to demonstrate the integrity of the peptide and preservation of biological activity after the lyophilization process. Results: Based on critical temperature characterization studies, the freeze-drying cycle was designed to reach a freezing temperature of around −40˚C and primary drying at 2˚C. Using 20 mg of mannitol, an intact and elegant lyophilized cake was obtained. PSMA-11 lyophilized kit was directly labeled with 68Ga eluate from 68Ge/68Ga GMP generators (ITG and Eckert & Ziegler) resulting in % RP > 95% at pH 4.0 to 4.5. The results obtained from in vitro and in vivo biological competition studies confirmed the preservation of PSMA-11 affinity for the receptor after lyophilization. Conclusion: A lyophilized formulation (Kit) of PSMA-11 was successfully obtained, which preserved the integrity and biological activity of the peptide and guaranteed radiolabeling efficiency.

关键词： 68Ga-PSMA-11 Prostate-Specific Membrane Antigen (PSMA) Ready-to-Use Kit GMP-Grade Generators Metastatic Prostate Cancer PET Imaging

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葡萄牙国家社科人文研究基础设施“ROSSIO”规划与实践

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数字人文研究 2023年第1期3卷 95-112页

作者：通萨洛·M.d.·西利亚阿娜·C.·格洛里亚安热拉·S.·莎尔格萝布鲁诺·艾梅达达涅尔·蒙特罗马科·R.d.·弗雷塔斯努诺·弗雷雷刘语丝(译) 新里斯本大学中世纪研究所新里斯本大学新里斯本大学当代历史研究所新里斯本大学语言学中心新里斯本大学科学和技术学院研究院里斯本INESC-ID信息和决策支持系统实验室美国布林莫尔学院考古系

ROSSIO基础设施(ROSSIO Infrastructure)是一个免费的开放获取的平台,旨在汇总、整理、连接葡萄牙高等教育机构和文化机构所提供的社会科学、艺术和人文领域的各类数字资源。文章介绍了ROSSIO基础设施的概况、其主要目标、参与机构,以... 详细信息

ROSSIO基础设施(ROSSIO Infrastructure)是一个免费的开放获取的平台,旨在汇总、整理、连接葡萄牙高等教育机构和文化机构所提供的社会科学、艺术和人文领域的各类数字资源。文章介绍了ROSSIO基础设施的概况、其主要目标、参与机构,以及所提供的服务--探索入口、数字展览与数据集、虚拟研究环境。这些服务依赖于元数据收集方案,将原机构所提供的数字对象的元数据引入到ROSSIO中。汇总的数据集被转换为关联数据,然后基于由ROSSIO开发和出版的受控词汇表进行数据丰富过程。文章将描述这一过程,包括其间所涉及的应用程序,以及它们如何交互操作。基于可发现、可访问、可互操作、可重用(FAIR)的原则,我们将进一步思考这些服务如何促进科学传播。

关键词：数字基础设施元数据聚合受控词汇表数字人文 ICT工具社会科学艺术和人文数字平台虚拟研究环境展览和数据集科学研究的公共传播

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