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Methods and parameters of melting curve analysis for identification of Leishmania species:A scoping review

Asian Pacific Journal of Tropical Medicine 2021年第12期14卷 528-542页

作者： Juliana J G ferreira Fernanda S Nascimento Glaucia E B Marcon Eros A de Almeida Sandra C B costa Laboratório de Diagnóstico de Doenças Infecciosas por Técnicas de Biologia Molecular Departamento de Clínica MédicaFaculdade de Ciências MédicasUniversidade Estadual de Campinas(UNICAMP)Campinas13083-887SPBrazil Fundação Oswaldo Cruz Mato Grosso do Sul(FIOCRUZ MS) Rua Gabriel Abrão92Jardim das NaçõesCampo Grande79081-746 MSBrazil Grupo de Estudos em Doença de Chagas Departamento de Clínica MédicaFaculdade de Ciências MédicasUniversidade Estadual de Campinas(UNICAMP)Campinas13083-887SPBrazil

Leishmaniasis is a set of diseases with a worldwide distribution that affects mainly economically underprivileged populations in developing countries. It has a major impact on public health, with a global cost of billions of dollars per year. The treatment and control of leishmaniasis vary according to the Leishmania species involved, which require reliable methods for species identification. Since most of the currently used methods have limitations, there is a need for assays that allow rapid, precise identification of the offending species. Real-time polymerase chain reactions in conjunction with dissociation curve analysis have been used to detect differences in the DNA composition of selected genes of Leishmania spp. Kinetoplast DNA is the main molecular target used because of its high copy number per parasite, but other targets have also been studied. As part of an effort to establish melting temperature standards for each target gene, we have reviewed the pertinent literature available in public databases, including Pub Med, Web of Science, Sci ELO and LILACS, using the keywords "Leishmania", "leishmaniasis", "realtime PCR", "melting temperature", and "melting curve", alone or in combination. After applying eligibility criteria, 27 articles were selected for analysis. A considerable variation in the methodologies analyzed was found regarding molecular targets, standardization of the methods, reproducibility and specificity. Because of this, statistical analysis was not performed. In most cases, the methods were able to differentiate the parasite at the subgenus level or few species regardless of the target chosen.

关键词： Leishmaniasis Melting temperature Real-time PCR

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Development of a System to Monitor the Quality of Electric Energy Generation in Micro-scale

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Journal of Energy and Power Engineering 2018年第8期12卷 402-407页

作者： Ernesto Guevara Leal Manicoba Eliel da costa Pinheiro Jacques Cousteau da Silva Borges Ricardo ferreira Pinheiro Filho Energy Laboratory IFRN(Federal Institute of EducationScience and Technology of Rio Grande do Norte)Natal-RNAv.Senador Salgado Filho 155959015-000Brazil

This work demonstrates a system of monitoring the quality of energy produced by micro-scale *** to the growth in the use of alternative energy generation systems as a means of supplying high demand situations in the network,the problems that these sources generate with respect to power quality such as voltage fluctuation,harmonic distortion and should be studied and *** at this need,we present a device capable of receiving voltage and current data,and through a software developed in the LabVIEW language present in real time information on energy quality,following parameters defined by the Brazilian Agency of Electric Energy.

关键词： Behavior of harmonics power factor power quality LabVIEW,ANEEL

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Contribution of inrush current to mechanical failure of power transformers windings

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High Voltage 2019年第4期4卷 300-307页

作者： Rafael M.R.Barros Edson Gda costa Jalberth F.Araujo Filipe L.Mde Andrade Tarso V.ferreira Department of Electrical Engineering Federal University of Campina GrandeAprigio Veloso St.882Campina GrandeBrazil

This work presents a study regarding the contribution of inrush current to the occurrence of mechanical fatigue in windings of a power transformer and,consequently,the impact of that phenomenon on the equipment's *** order to perform the study,electromagnetic transient simulations were used to determine the inrush current in a three-phase transformer ***,three-dimensional simulations,based on the finite element method,were performed to determine the electromechanical stresses in the equipment windings,under the effect of the inrush ***,the methods of critical plans and Palmgren–Miner were applied to perform the fatigue *** the results,the impact of inrush current on the integrity of the transformer's windings was determined,as well as,to estimate the reduction of lifespan of the equipment due to inrush *** number of inrushes needed for the total reduction of lifespan of the equipment due to mechanical fatigue was also *** results can be used in the transformers design stage,with the purpose of assisting the evaluation of mechanical withstand of equipment,which can minimise the possibility of failure and increase its lifespan.

关键词： winding fatigue power

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Determination of the Bromine, Manganese and Antimony in Nicotiana tabacum Solanaceae by Using the Neutron Activation Analysis Technique

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Materials Sciences and Applications 2022年第2期13卷 91-106页

作者： Tufic Madi Filho Elson Barros ferreira José Roberto Berretta Maria da Conceição costa Pereira Instituto de Pesquisas Energéticas e Nucleares S&atilde o Paulo Brazil Universidade Paulista—UNIP-SP Software Engineering Research Group Paulista University UNIP Campus Tatuapé S&atilde o Paulo Brazil Secretaria da Educaç ã o do Estado de Sã o Paulo S&atilde o Paulo Brazil

Tobacco addiction has been mentioned as a leading cause of preventable illnesses and premature disability. Smoking is the main cause of lung cancer and one of the factors that most contribute to the occurrence of heart diseases, among others. The herbaceous species Nicotiana tabacum is a plant of the solanaceae family used for tobacco production. Some authors have conducted research about heavy metals and the toxicity of tobacco. It is, frequently, found in low concentrations in the ground, and superficial and underground waters, even though they do not have environmental anthropogenic contributions. However, with the increase of industrial activities and mining together with the agrochemical use of contaminated organic and inorganic fertilizers, an alteration of the geochemical cycle occurs. As a consequence, the natural flow of these materials increases and is released into the biosphere, where they are often accumulated in the superior layer of the ground, accessible to the roots of the plants. During planting and plant development, fertilizers and insecticides, including organochlorines and organophosphates, are used;consequently, the smoke from cigarette smoking presents various toxic substances, such as bromine (Br), manganese (Mn) and antimony (Sb), elements studied in this work. The procedures for the preparation of the samples were carried out in our laboratories and submitted to irradiation with thermal neutrons at Nuclear and Energy Research Institute (IPEN/CNEN-SP), in the Atomic Energy Institute IEA-R1 research reactor. The irradiated material was, then, analyzed by gamma spectrometry, using a high purity germanium detector (HPGe).

关键词： Nicotiana tabacum Bromine Manganese Antimony Neutron Activation Analysis

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超声波与人工智能在现场绝缘诊断中的应用(英文)

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高电压技术 2012年第8期38卷 1842-1847页

作者： ferreira T V Germano A D Silva K M costa E G Department of Electrical Engineering Federal University of Campina GrandeCampina Grande Institute for High Voltage Technology and Power Transmission Technische Universitt München Department of Electrical Engineering University of Brasília

This work studies the feasibility of implementing a system for diagnosis of electrical insulation in the field based on ultrasonic noise and artificial neural *** system,proved functional under laboratory conditions,extracts spectral information from the ultrasonic noise emitted by the corona discharges in electric equipments,and correlates it with previously defined degrees of *** achieve this classification,artificial neural networks are *** results show the viability of the method in the site;nevertheless they also show that its reliability is proportional to the size and diversity of the available database.

关键词：局部放电射频信号放电量高电压技术

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Demand Charge under Nonsinusoidal Conditions

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Journal of Energy and Power Engineering 2013年第2期7卷 375-380页

作者： Eduardo da costa Sousa Ezequiel Junio de Lima Augusto Wohlgemuth Fleury Veloso da Silveira Sergio ferreira de Paula Silva School of Electrical Engineering Brazilian Federal University of U-berlandia Uberldndia 38408-100 Minas Gerais Brazil

This paper aims to present a new point of view about the active power measurement, for billing purposes, measured at the PCC （point of common coupling） between the utility and the consumer when harmonic distortions are involved. Depending on theorigin of it, the active power can result in higher or lower values in comparison to the fundamental component. The consequences are higher costs for the consumer or losses for the electric utility. Using computational simulations and theoretical analysis, these aspects are evaluated and compared.

关键词： Harmonic distortions demand charge power quality active power harmonic direction.

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Tracheal Intubation Difficulties: Systematic Literature Review

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Journal of Biosciences and Medicines 2022年第5期10卷 95-104页

作者： Vanessa ferreira Dias Duarte da costa Mariana Ronchesel Barauna Camila dos Santos Leite Oscar César Pires Laboratory of Pharmacology and Physiology Medicine Department Taubaté University Taubaté Brazil Laboratory of Immunopharmacology and Molecular Biology S&atilde o Francisco University Medical School Bragan&ccedil a Paulista Brazil

Background: Tracheal intubation is a common procedure in medical practice, being performed mainly in surgical centers and emergencies in trauma patients. Objective: This systematic literature review aims to understand the main difficulties and complications related to tracheal intubation. Methods: Systematic literature review, conducted in the Virtual Health Library databases between 2016 and 2021, restricted to articles in English and Portuguese with the descriptors: Airway, Difficult intubation, and Complications. After reading the abstracts, 34 articles were found, and, after reading them, 9 articles were selected. Results: This review analyzed 9 scientific studies that rigorously met the previously established characteristics in the sample selection. The synthesis included the following aspects (author/year of publication, article title, objective, type of study, and database. Discussion: Several factors that influence the success of intubation were identified, such as technical, professional, and patient issues. In addition, measures to minimize the risks involving the procedure were highlighted. Conclusion: Tracheal intubation is a common procedure in surgical centers and emergencies. However, its risks, while low, should not be ignored. Unsuccessful procedures can occur and have consequences for the patient, such as trauma to the upper airway.

关键词： Airway Difficult Intubation Complications

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Energy Efficiency of Reactive Dynamic Compensators

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Journal of Energy and Power Engineering 2016年第3期10卷 191-198页

作者： Rodrigo Nobis da costa Lima Sergio ferreira de Paula Silva Antonio Carlos Delaiba Ezequiel Junio deLima Energy Rationalization and Power Quality Group Faculdade de Engenharia Eletrica Universidade Federal de Uberldndia Uberlandia 38. 400-902 Brazil Instituto Federal de Educacao Ciencia e Tecnologia do Sul de Minas Gerais Po~os de Caldas 37713-100 Brazil

A parameter that allows an evaluation of power quality transmitted, or distributed, between energy source and the final user is electric system power factor. Among other aspects, a bigger power factor, close to unit value, relieves operational conditions of lines and cables, besides, it improves feeder＇s voltage behavior. Due to load variation along the day, the dynamic compensation of power factor allows maintaining this parameter close to the ideal. This paper brings a study about a reactive dynamic compensator based on the voltage control in a capacitive element, varying the reactive energy in accordance with the system demand, everything from the energy efficiency point of view. In distribution systems, the losses due to this variable compensation can be lower than in other compensation methods and also the voltage presents a better behavior, justifying its application.

关键词： Reactive dynamic compensator power factor correction losses energy efficiency tap changing.

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Organic Vegetables Quality Arising from the Brazilian Family-Run Farm

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Food and Nutrition Sciences 2014年第15期5卷 1538-1544页

作者： Thadia Turon costa da Silva Verona Borges ferreira Silvia Magalhaes Couto Armando Ubirajara Oliveira Sabaa Srur Department of Food Technology Federal Rural University of Rio de JaneiroRio de JaneiroBrazil Nutrition Institute Federal University of Rio de JaneiroRio de JaneiroBrazil Research Group in Fruits and Vegetables Processing CNPqUFRJRio de JaneiroBrazil

The present study has its aim to evaluate the different dimensions of organic vegetables quality from the Brazilian family-run farm, especially the evaluation of composition, antioxidant activity, microbiological characteristics and pesticide residues. There were evaluated carrot, broccoli, kale and white cabbage grown by organic farmers in the highlander region of Rio de Janeiro state. There were determined humidity, lipids grade, total protein content, mineral residues (ashes), soluble and insoluble fibers, soluble solids, total acidity, pH, and pesticide residues. The antioxidant activity was determined using DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radical removal. Among the analyzed vegetables, the effectiveness of the antioxidant capacity was obtained, in decreasing order, by broccoli, kale, white cabbage and carrot. According to the microbiological parameter, evaluated vegetables have shown satisfactory sanitary quality. Pesticide residues were not found above the detection limit for both authorized and unauthorized analyzed crops.

关键词： Organic Food Vegetables Pesticide Antioxidant Capacity Food Composition

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Hepatic“pregnancy”:hepatocellular carcinoma with extensive extrahepatic growth

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Hepatobiliary Surgery and Nutrition 2023年第4期12卷 641-642页

作者： Ana Rita ferreira Bárbara Castro Jose daniel Martins João Varanda Alexandre costa Bela Pereira Manuel Oliveira General Surgery Centro Hospitalar Vila Nova de Gaia/EspinhoVila Nova de GaiaPortugal

A female in her 50s,no previous personal history,presented to the Emergency Department with a 7 months history of progressively worsening abdominal diffuse pain associated with increasing abdominal *** physical examin... 详细信息

关键词： pregnancy Hepatic Figure

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