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Improving ITs sequence data for identification of plant pathogenic fungi

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Fungal Diversity 2014年第4期67卷 11-19页

作者： R.Henrik Nilsson Kevin D.Hyde Julia Pawlowska Martin Ryberg Leho Tedersoo Anders Bjornsgard Aas siti A.Alias Artur Alves Cajsa Lisa Anderson Alexandre Antonelli A.Elizabeth Arnold Barbara Bahnmann Mohammad Bahram Johan Bengtsson-Palme Anna Berlin sara Branco Putarak Chomnunti Asha Dissanayake Rein Drenkhan Hanna Friberg Tobias Guldberg Froslev Bettina Halwachs Martin Hartmann Beatrice Henricot Ruvishika Jayawardena Ari Jumpponen Havard Kauserud sonja Koskela Tomasz Kulik Kare Liimatainen Bjorn D.Lindahl Daniel Lindner Jian-Kui Liu sajeewa Maharachchikumbura Dimuthu Manamgoda svante Martinsson Maria Alice neves Tuula Niskanen stephan Nylinder Olinto Liparini Pereira Danilo Batista Pinho Teresita M.Porter Valentin Queloz Taavi Riit Marisol sánchez-García Filipe de sousa Emil stefańczyk Mariusz Tadych susumu Takamatsu Qing Tian Dhanushka Udayanga Martin Unterseher Zheng Wang saowanee Wikee Jiye Yan Ellen Larsson Karl-Henrik Larsson Urmas Koljalg Kessy Abarenkov Department of Biological and Environmental sciences University of GothenburgBox 46140530 GothenburgSweden Institute of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand school of science Mae Fah Luang UniversityChiang Rai 57100Thailand Department of Plant systematics and Geography Faculty of BiologyUniversity of WarsawAl.Ujazdowskie 400-478 WarsawPoland Department of Organismal Biology Uppsala UniversityNorbyvagen 18D75236 UppsalaSweden Institute of Ecology and Earth sciences University of TartuLai 40Tartu 51005Estonia Microbial Evolution Research Group University of OsloBlindernveien 310371 OsloNorway Institute of Biological sciences University of Malaya50603 Kuala LumpurMalaysia Department of Biology CESAMUniversity of Aveiro3810-193 AveiroPortugal school of Plant sciences The University of Arizona1140 E South Campus DriveForbes 303TucsonAZ 85721USA Laboratory of Environmental Microbiology Institute of Microbiology ASCRVídeňská108314220 Prague 4Czech Republic Department of Infectious Diseases Institute of BiomedicineSahlgrenska AcademyUniversity of GothenburgGuldhedsgatan 1041346 GoteborgSweden Department of Forest Mycology and Plant Pathology Swedish University of Agricultural SciencesBox 702675007 UppsalaSweden University of California at Berkeley 321 Koshland Hall University of CaliforniaBerkeleyCA 94720-3102USA Agriculture and Forestry sciences Beijing 100097China Institute of Forestry and Rural Engineering Estonian University of Life SciencesKreutzwaldi551014 TartuEstonia Natural History Museum of Denmark Øster Voldgade 5-71350 København KDenmark Institute for Genomics and Bioinformatics Graz University of Technology8010 GrazAustria Core Facility Bioinformatics Austrian Centre of Industrial Biotechnology8010 GrazAustria Forest soils and Biogeochemistry Swiss Federal Research Institute WSLZuercherstrasse 1118903 BirmensdorfSwitzerland Molecular Ecology Institute for Sustainability SciencesAgroscopeReckenholzstrasse 191

Plant pathogenic fungi are a large and diverse assemblage of eukaryotes with substantial impacts on natural ecosystems and human *** taxa often have complex and poorly understood life cycles,lack observable,discriminatory morphological characters,and may not be amenable to in vitro *** a result,species identification is frequently ***(DNA sequence)data have emerged as crucial information for the taxonomic identification of plant pathogenic fungi,with the nuclear ribosomal internal transcribed spacer(ITs)region being the most popular ***,international nucleotide sequence databases are accumulating numerous sequences of compromised or low-resolution taxonomic annotations and substandard technical quality,making their use in the molecular identification of plant pathogenic fungi *** we report on a concerted effort to identify high-quality reference sequences for various plant pathogenic fungi and to re-annotate incorrectly or insufficiently annotated public ITs sequences from these fungal lineages.A third objective was to enrich the sequences with geographical and ecological *** results-a total of 31,954 changes-are incorporated in and made available through the UNITE database for molecular identification of fungi(http://***),including standalone FAsTA files of sequence data for local BLAsT searches,use in the next-generation sequencing analysis platforms QIIME and mothur,and related *** present initiative is just a beginning to cover the wide spectrum of plant pathogenic fungi,and we invite all researchers with pertinent expertise to join the annotation effort.

关键词： Phytopathogenic fungi Molecular identification ITs Taxonomy Annotation

输尿管支架用镁合金丝材在模拟尿液中的腐蚀行为

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输尿管支架用镁合金丝材在模拟尿液中的腐蚀行为

Response to Commentary by Dr. Matthew J. Clarkson on

第十一届全国腐蚀与防护大会

作者：梅迪王成 Maria Nienaber Margarida Pacheco Alexandre Barros sara neves Rui L.Reis 朱世杰 Jan Bohlen Dietmar Letzig 关绍康 Mikhail.L.Zheludkevich sviatlana V.Lamaka 郑州大学 University of Minho

可生物降解的金属材料是用于制造生物医学植入装置的有前途的材料[1-2]。近年来,可降解镁合金作为输尿管支架的适用性和体内相容性已通过动物试验得到初步证实[3]。尽管初步结果表明镁合金是制造输尿管支架的有前途的候选材料之一[4-5]... 详细信息

可生物降解的金属材料是用于制造生物医学植入装置的有前途的材料[1-2]。近年来,可降解镁合金作为输尿管支架的适用性和体内相容性已通过动物试验得到初步证实[3]。尽管初步结果表明镁合金是制造输尿管支架的有前途的候选材料之一[4-5],但相关的研究基础尚不牢固。镁在尿道环境中的降解行为仍然没有被完全理解。本工作研究了人造尿液中镁合金的腐蚀机理,有助于更好地理解镁合金作为输尿管植入物的降解行为。经模拟尿液浸泡72小时后纯Mg和Mg-Zn丝材腐蚀后的横截面形貌如图1所示。Mg-1Zn丝材表现出严重的局部腐蚀(图1中的箭头所指(a1-a3))。纯Mg丝材中也存在局部腐蚀,但不如Mg-1Zn丝材明显。连续EIs测试是分析形成的腐蚀产物层的演变和屏障保护能力的有效方法。Mg-1Zn丝材和纯Mg丝材在模拟尿液中浸泡72小时的Bode图如图2所示。两个样品的初始Bode图(浸泡15分钟后)非常相似,由中频和低频时间常数组成。两个样本的低频阻抗模量在整个浸没期间逐渐增加。随着时间的增加,浸入24小时(纯Mg丝材)或48小时(Mg-1Zn丝材)后,在高频范围(10 kHz至100kHz内)会出现一个附加的时间常数。此后高频阻抗模量也随着高频时间常数的增加而逐渐增加。连续EIs证实了浸入模拟尿液期间两种Mg丝材上腐蚀产物的层状结构。输尿管支架是可生物降解镁合金的一个相对较新的应用领域。在这项研究中发现经模拟尿液浸泡后的Mg表面腐蚀产物具有两层结构:外层MgNHPO·6HO和内层Mg(OH)。具有一定保护性的外层MgNHPO·6HO产物减缓了Mg的腐蚀,但这一现象显示了裸Mg支架在泌尿系统环境中的结壳风险。

关键词：镁丝输尿管支架模拟尿液腐蚀行为

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Journal of sport and Health science 2024年第5期13卷 670-671页

作者： Hugo de Luca Corrêa Lysleine Alves Deus Dahan da Cunha Nascimento Nicholas Rolnick Rodrigo Vanerson Passos neves Andrea Lucena Reis Thais Branquinho de Araújo Carmen Tzanno-Martins Fernanda silveira Tavares Luiz sinésio silva Neto Cláudio Avelino Rodrigues santos Paolo Lucas Rodrigues-silva Fernando Honorato souza Vitória Marra da Motta Vilalva Mestrinho Rafael Lavarini dos santos Rosangela Vieira Andrade Jonato Prestes Thiago dos santos Rosa Post-graduate Program of Physical Education Catholic University of BrasıliaBrasılia 71966-700Brazil The Human Performance Mechanic Lehman CollegeNew YorkNY 10011USA Clinical Group Home Dialysis Center and RenalClass Sao Paulo 01239-040Brazil Department of Medicine Catholic University of BrasıliaBrasılia 71966-700Brazil Faculty of Medicine Federal University of TocantinsTocantins 77001-090Brazil Embrapa Genetic Resources and Biotechnology Brasılia 70770-917Brazil g Graduate Program of Genomic sciences and Biotechnology Brasılia 71966-700Brazil

Dear editor:We sincerely thank Matthew *** for his insightful commentary1 on our recent publication in the Journal of sport and Health science.2 Clarkson's pioneering work in the development and application of blood flow restriction (BFR) exercise protocols has undeniably laid the groundwork for subsequent investigations in this field,including our own.(3-6)His commentary not only highlights the relevance and timeliness of our study but also offers valuable perspectives on refining safety considerations for intradialytic BFR exercise among hemodialysis patients.

关键词： patients application flow

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医院小丑在儿科症状管理中的效果

英国医学杂志中文版 2021年第10期24卷 606-608页

作者： Lufs Carlos Lopes-Junior Emiliana Bomfim Karin Olson Eliane Tatsch neves Denise sayuri Calheiros silveira Michelle Darezzo Rodrigues Nunes Lucila Castanheira Nascimento Gabriela Pereira-da-silva Regina Aparccida Garcia Lima 蔡思雨(译) 彭晓霞(校) Federal University of Espirito santo Avenida Marechai CampusES.Brazil University of saskatchewan College of Medicine.SaskatoonSKCanada University of Alberta EdmontonABCanada Federal University of santa Maria Santa Maria.RSBrazil Federal Institute of Espirito santo LinharesESBrazil Rio de Janeiro state University Rio de Janeiro.RJ.Brazil University of sao Paulo at Ribeirao Prelo College of Nursing SPBrazil 国家儿童医学中心首都医科大学附属北京儿童医院临床流行病学与循证医学中心

目的基于随机对照试验和非随机对照试验产生的证据,在因急慢性疾病住院的儿童和青少年中,评价医院小丑对一系列症状群的干预效果。研究设计基于随机和非随机对照试验的系统综述。数据来源美国国立医学图书馆(MEDLINE).美国科学情报研究... 详细信息

目的基于随机对照试验和非随机对照试验产生的证据,在因急慢性疾病住院的儿童和青少年中,评价医院小丑对一系列症状群的干预效果。研究设计基于随机和非随机对照试验的系统综述。数据来源美国国立医学图书馆(MEDLINE).美国科学情报研究所数据库(IsI of Knowledge).考科蓝对照试验注册中心(Cochrane Cental Registerof Controled Trials)、science Diret全文数据库、sopus数据库、美国心理协会数据库(American Psychalogical Association PeyeINFO)、护理和健康文献累积指数数据库(Curmulative Index to Nursing and Alied Heauth Literature),以及拉丁美洲和加勒比健康科学文献数据库(Lain Ameriean and Caribbean Health sciences Literature)。研究筛选经間行评议后筛选随机和非随机对照试脸,纳人标准如下:因急性或慢性疾病人院的儿童和青少年,比较医院小丑与标准护理的研究,此外研究将医院小丑对住院儿童和青少年症状管理的影响作为主要结局指标。数据提取与整合由两名研究者独立完成研究筛选,数据摘录,以及偏倚风险评价。方法学评价也由两名研究者独立进行,评价工具有:Jadnd量表、随机对照试验的偏倚风险评估工具(RoB 2)和非随机对照试验的偏倚风险评估工具(ROBINs-I)。结果24项研究(n=1612)符合纳入标准,对其进行数据摘录和分析。大多数研究是随机对照试验(n=13)。最常分析的症状是焦虑(n=13),其次是疼痛(n=9)、心理和情绪反应以及幸福感(n=4)、压力(n=4)、癌症相关疲劳(n-3)和哭泣(n=2)。5项研究使用生物标志物(主要为皮质醇)来评估医院小丑对压力或疲劳的影响。方法学评价显示,大多数随机对照试验(n=11;85%)存在潜在偏倚风险,2项试验具有高度偏倚风险。ROBINs-I评价结果显示,大多数非随机对照试验(n=6;55%)存在中度偏倚风险。研究表明,无论父母是否在场,医院小丑出现时,儿童和青少年在一系列医疗程序中的焦虑显著降低,心理调节也得到改善(P<0.05)。3项针对慢性病患者的研究显示,医院小丑的干预有明显获益,显著减少了患儿的压力、疲劳、疼痛和痛营(PC0.05)。结论这些研究表明,在医疗程序术前诱导麻醉,以及慢性病常规护理中进行医院小丑干预,可能是症状管理的有效策略。此外,与接受标准护理的患儿相比,医院小丑干预可能有助于改善患有急慢性疾病的儿童和青少年的心理健康状态。

关键词：急慢性疾病方法学评价慢性病患者心理健康状态诱导麻醉症状群情报研究所文献数据库

Highlighting COVID-19:What the imaging exams show about the disease

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World Journal of Radiology 2021年第5期13卷 122-136页

作者： Lorena sousa de Carvalho Ronaldo Teixeira da silva Júnior Bruna Vieira silva Oliveira Yasmin silva de Miranda Nara Lúcia Fonseca Rebouças Matheus sande Loureiro samuel Luca Rocha Pinheiro Regiane santos da silva Paulo Victor silva Lima Medrado Correia Maria José souza silva sabrina neves Ribeiro Filipe Antônio França da silva Breno Bittencourt de Brito Maria Luísa Cordeiro santos Rafael Augusto Oliveira sodré Leal Márcio Vasconcelos Oliveira Fabrício Freire de Melo Department of Instituto Multidisciplinar em saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil

Coronavirus disease 2019(COVID-19),a global emergency,is caused by severe acute respiratory syndrome coronavirus *** gold standard for its diagnosis is the reverse transcription polymerase chain reaction,but considering the high number of infected people,the low availability of this diagnostic tool in some contexts,and the limitations of the test,other tools that aid in the identification of the disease are *** this scenario,imaging exams such as chest X-ray(CXR)and computed tomography(CT)have played important *** is useful for assessing disease progression because it allows the detection of extensive consolidations,besides being a fast and cheap *** the other hand,CT is more sensitive for detecting lung changes in the early stages of the disease and is also useful for assessing disease *** note,ground-glass opacities are the main COVID-19-related CT *** emission tomography combined with CT can be used to evaluate chronic and substantial damage to the lungs and other organs;however,it is an expensive *** ultrasound(LUs)has been shown to be a promising technique in that context as well,being useful in the screening and monitoring of patients,disease classification,and management related to mechanical ***,LUs is an inexpensive alternative available at the ***,magnetic resonance imaging,although not usually requested,allows the detection of pulmonary,cardiovascular,and neurological abnormalities associated with ***,it is important to consider the challenges faced in the radiology field in the adoption of control measures to prevent infection and in the follow-up of post-COVID-19 patients.

关键词： sARs-CoV-2 COVID-19 Pneumonia Pandemic Radiology Tomography

BiFeO_(3) codoping with Ba,La and Ti:Magnetic and structural studies

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Journal of Advanced Dielectrics 2015年第4期5卷 53-60页

作者： O.García-Zaldívar s.Díaz-Castañón F.J.Espinoza-Beltran M.A.Hernández-Landaverde G.López J.Faloh-Gandarilla F.Calderón-Piñar Facultad de Física-Instituto de Ciencia y Tecnología de Materiales Universidad de La Habana San Láazaro y L VedadoLa Habana 10400Cuba CINVEsTAV-Unidad Querétaro Libramiento Norponiente No.2000 Real de JuriquillaQuerétaroQro.76230México Instituto Latino-Americanode Ciênciasda Vidaeda Natureza Universidade Federalda Integração Latino-Americana (ILCVN-UNILA)Avenida Tancredo NevesPTIBloco 6 CEP:85866-000-Foz do Iguaçu-ParanaBrazil División Materiales Avanzados Instituto Potosinode Investigación Científica Tecnológica san Luis Potosí CP78216Méexico

Conventional solid state reaction method,from oxides and carbonates,was employed to prepare bismuth(Bi)-based multiferroic *** undoped BiFeO_(3)(BFO)and the codoped system with Ba,La and Ti(Bi_(1-x)Ba_(x)Fe_(1-y)Ti_(y)O_(3),Bi_(1-x-z)Ba_(x)LazFe_(1-y)TiyO_(3))with x,y,z=0.1 were prepared stoichiometrically and sintered at two different *** structural and magnetic properties were investigated at room *** measurements confirm the obtaining of the rhombohedral perovskite structure of the BFO family *** the undoped system,some reflections of undesired phases are present for two different sintering temperatures,while for the doped system only one phase is present for both *** magnetic characterization at room temperature revealed remarkable differences between the ceramic *** results show that for undoped BFO system,spontaneous magnetization is not observed at room ***,in doped one,a well-defined ferromagnetic behavior is observed at room temperature,possible,due to the suppression of the spatially modulated spin structure of BFO promoted by the reduction of the rhombohedral distortion and the weakening of the Bi-O *** XPs results confirm the presence of oxygen vacancies and the coexistence of Fe^(3+)and Fe^(2+) in all the studied *** measurements reveal that the dopant incorporation has not a direct effect in Neel temperature but possibly yes in ferroelectric-paraelectric transition.

关键词： Multiferroics XPs magnetic bismuth ferrite BiFeO_(3)

超声波提取后，用原子吸收光谱法测定鱼饲料中的矿物元素

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饲料与畜牧（新饲料） 2009年第2期 56-56页

作者： neves,R.C.F. Moraes,P.M. saleh, M.A.D. Loureiro,V.R. 雷蕾(译者) 李菊(校者)

本研究推荐一种测定鱼饲料样品中钙、镁、锰、锌的方法，即采用超声波提取样品，然后用原子吸收光谱法（FAAs）进行后续的量化。提取液是0．10mol／L的HCl，建立的最佳萃取条件如下：100mg样品；样品粒度小于60μm；3个周期的超声时间为... 详细信息

本研究推荐一种测定鱼饲料样品中钙、镁、锰、锌的方法，即采用超声波提取样品，然后用原子吸收光谱法（FAAs）进行后续的量化。提取液是0．10mol／L的HCl，建立的最佳萃取条件如下：100mg样品；样品粒度小于60μm；3个周期的超声时间为10s；超声功率为102W。这种方法运用于研究尼罗罗非鱼幼鱼日粮饲料样品中这些营养素的消化率。测得的结果与在金属养分提取过程中鱼饲料样品矿化结果一致。

关键词：矿物元素的吸收原子吸收光谱法鱼类营养超声波

Chemical Exposure Hazardous for Fish Hyphessobrycon eques through the Incorrect Release of Oil in Amazon Region

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Journal of Fisheries science 2020年第2期2卷 19-23页

作者： Natalino da Costa sousa Arthur dos santos da silva Keber santos Costa Junior Francisco Alex Lima Barros Geisy Correa de Oliveira Pedro Rodrigo Nery de souza Claudmilson Rodrigues de Mesquita Bruna de jesus santos Alexander Damaceno Lima santos Winnícius Muniz dos santos sá Nayara siqueira dos santos Márcia Valéria silva do Couto Federal University of Pará(UFPA) Campus BragançaAlameda Leandro RibeiroBragança68600-000PABrazil Federal University of Pará(UFPA) Campus de TucuruíRua Itaipu36-Vila PermanenteTucuruí68464-000PABrazil Federal University of de sergipe(UFs) Av.Marechal Rondons/n-Jardim Rosa ElzeSão Cristóvão49100-000SEBrasil Faculdade Pio Décimo Av.Pres.Tancredo neves 5655-Jabotiana49095-000Aracaju–SE.Brazil

Incorrect discard of oil used by fishing ship in the brazilian north region has become a common *** the possible hazard to the aquatic organisms,still missing scientific data about their ***,this study aimed to evaluate the stress and lethality caused by lubricant oil(FsAOLU)on fish Hyphessobrycon ***,it was used six different concentration diluted in water(0,22,24,26,28,30 and 32%of oil)and three replaces during 96 *** the end or during the experiment(with dying fish),it was collected blood samples(cutting the caudal fin)to determine physiological *** FsAOLU showed mean lethal dose(LD50-96h)of 27.36%,classified as toxic causing alterations in glucose values from the 26%.The greater dilution of FsAOLU(32%)increased 115%in glucose values when compared to the ***,lubricant oil when discarded in water,it present hazard to the aquatic organisms causing stress and mortality for fish being necessary adequate management to discard of this residue.

关键词： Toxicity FsA sensibility stress