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The Optimization of Aspergillus ***4 Tannase Production under Submerged Fermentation

Advances in Microbiology 2014年第3期4卷 143-150页

作者： Alessandra Goncalves de Melo Rayssa Cristina Faria Pedroso Luis Henrique souza Guimaraes josé Guilherme Lembi Ferreira Alves Eustáquio souza Dias Mário Lúcio Vilela de Resende Patrícia Gomes Cardoso department of Biology Sector of MicrobiologyFederal University of LavrasLavrasBrazil department of Biology University of Sao PauloRibeirao PretoBrazil department of Food Science Federal University of LavrasLavrasBrazil department of Phytopathology Federal University of LavrasLavrasBrazil

Tannase is a hydrolytic enzyme that is involved in the biodegradation of tannins and it has biotechnological potential in the pharmaceutical, chemical, food and beverage industries. Microorganisms, especially filamentous fungi, are important tannase producers. The aims of this work were to find a potential tannase producer and to improve the cultivation conditions. Three Aspergillus species (A. japonicus 246A, A. tamarii 3 and Aspergillus sp. GM4) were investigated in different culture media (Adams, Czapeck, Khanna, M5 and Vogel) and inducers (1% and 2% tannic acid;1% green tea;1% methyl gallate;1% gallic acid). Aspergillus sp. GM4 and Adams medium were selected. The tannase production by Aspergillus sp. GM4 in Adams medium was induced in the presence of 2% (w/v) tannic acid and gallic acid as carbon sources, while green tea was not able to induce tannase production. The Plackett-Burman screening design was performed with the variables MgSO4, KH2PO4, yeast extract, tannic acid, agitation rate and salt solution. The variables MgSO4 and agitation rate were selected for the optimization of tannase production using a Central Composite Rotatable design. Under optimized conditions, a 2.66-fold increase in the enzyme production was observed with small modifications in the medium composition.

关键词： Aspergillus sp. Response Surface Methodology Submerged Fermentation Tannase Tannic Acid

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Extrahepatic right hepatic duct diverticulum: a rare entity

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Hepatobiliary & Pancreatic Diseases International 2010年第2期9卷 213-215页

作者： Eduardo SM Fernandes Raquel L Bernardo Moacir M Fernandes Rogério MC Araújo Renato Sebbe Asterio Monte joséF Coelho Antonio AP souza Joaquim Ribeiro Filho department of General Surgery Section of Hepatobiliary Surgery and TransplantationRio de Janeiro Federal University department of Digestive Surgery and Gastroenterology Pio XII General Hospital

BACKGROUND:Douglas described choledochal cysts in 1852 and Todani proposed an anatomy-based classification in 1977. The classification is the most extensively used,but does not include some rare *** present a case of hepatic duct diverticulum,one of the variations,and discuss its diagnosis and treatment. METHODS:A 57-year-old woman presented with upper abdominal pain and discomfort associated with nausea and *** was finally diagnosed with cholelithiasis and right hepatic duct diverticulum. RESULT:The patient underwent resection of the hepatic duct diverticulum and cholecystectomy,and was asymptomatic 26 months after surgery. CONCLUSIONS:Hepatic duct diverticulum is a rare form of choledochal cyst,not included in Todani’s classification. Todani’s classification including this and other uncommon variations of choledochal cysts must be *** best diagnostic imaging methods and treatment for choledochal cysts must be defined.

关键词： choledochal cysts hepatic duct diverticulum Todani's classification intrahepatic diverticulum

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Metastasis to Paranasal Sinuses as the First Presenting Sign of Renal Cell Carcinoma

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Open Journal of Urology 2012年第1期2卷 28-31页

作者： Gustavo Ruschi Bechara josé Anacleto Dutra Resende Júnior Hélio Assuncao Gouveia Thomás Accioly de souza department of Urology Hospital Federal da Lagoa Rio de Janeiro Brazil

The clear cell carcinoma is a relatively rare tumor, represented approximately 85% by renal cell carcinoma (RCC), but has a characteristic of early metastatic, which sometimes spread to unusual places. Metastatic tumors in the paranasal sinuses are very rare. This case report a 65 years-old patient who presented sinusitis and epitaxis as a first sign of renal cell carcinoma with metastasis to the right maxillary sinus, which was opereted at Lagoa Hospital—RJ and strengthen the inclusion of this disease in the differential diagnosis of sinus disease. The renal cell carcinoma is a relatively rare tumor, representing approximately 3% of malignant tumors. About a third of patients with renal cell carcinoma have metastasis at diagnosis. The sinuses are a rare location of metastasis. The maxillary sinuses and the paranasal sinuses are most commonly affected by metastatic tumors to the sinuses region. The most frequent primary tumor to metastasis to paranasal sinuses is the renal cell carcinoma. This report highlights the rarity of the case and the importance in the differential diagnosis of sinus disease.

关键词： Renal Cell Carcinoma Metastasis Sinuses Epistaxis

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Growth of Pepper under Different Concentration of the Solutions Used in the Hydroponic System

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Agricultural Sciences 2018年第8期9卷 925-935页

作者： Felipe Guedes de souza Guilherme de Freitas Furtado Antonio Ramos Cavalcante Lucia Helena Garófalo Chaves josely Dantas Fernandes josé Amilton Santos Júnior department of Agricultural Engineering Federal University of Campina Grande Campina Grande Brazil department of Agricultural Engineering Federal Rural University of Pernambuco Recife Brazil

Water availability is a fundamental condition for sustainable agriculture. However, a satisfactory water condition is not always possible in terms of quantity and quality in certain regions, such as the Brazilian semi-arid region. Thus, the purpose of this extremely important research was to evaluate the growth of cv. All Big with six combinations of wastewater plus saline (WW + SW) and two nutrient solution percentages, in a low-cost hydroponic system. The experiment was carried out in a greenhouse at the department of Agricultural Engineering at Federal University of Campina Grande. A completely randomized experimental design was used, with treatments arranged in a factorial scheme (6 × 2), with 5 replications, being the treatments composed by the combination of six proportions of wastewater with saline water (50%/50%;60%/40%;70%/30%;80%/20%;90%/10%;100% of wastewater) and two nutrient solution concentration (80% and 100%), totalizing 60 experimental units. The biometric variables were analyzed at 55 and 96 days after sowing, namely: plant height (PH), stem diameter (SD), leaf number (LN) and leaf area (LA). The interaction between the concentration of the nutrient solution and the ratio of the saline water with wastewater was influenced in the behavior of peppers (cv. All Big) biometric variables. The nutrient solution prepared at 80% of its normal concentration had better performance in the development of plants when mixed with the proportions of water containing more wastewater. The proportion 30% saline water and 70% wastewater (30/70) with concentration of 80% of the nutrient solution obtained better satisfactory results in the studied biometric variables.

关键词： Capsicum annuum L. Saline Water Wastewater Nutrient Solution

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Assessment of the Total Organic Carbon and Conductivity in Consecutive Dyeings with Reactive Dyestuffs Using Treated Effluent by UV/H202 Photocatalysis

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Journal of Chemistry and Chemical Engineering 2014年第2期8卷 139-144页

作者： Leite Adilson da SilvaI Barco Luiz Trindade Nelson barros Araujo Mauricio de Campos Rosa Jorge Marcos Tambourgi Elias Basile Santana josé Carlos Curvelo Chemical Textile Post Graduation department College of Technology SENAI Antoine Skaf $6o Paulo 03008-020 Brazil Textile and Fashion Post Graduation Program S6o Paulo University S6o Paulo 03828-000 Brazil College of Chemical Engineering State University of Campinas Campinas 13083-852 Brazil Production Engineering Post Graduation Program Nove de Julho University Sao Paulo 05001-100 Brazil

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Quality of <i>Jatropha curcas</i>L. Seeds Harvested at Different Stages of Fruit Maturation

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American Journal of Plant Sciences 2014年第18期5卷 2716-2725页

作者： Cristiane de Oliveira Veronesi Cristiano Marcio Alves de souza Ademar Pereira Serra Leidy Zulys Leyva Rafull Cesar josé da Silva Vinicius de Vito Ros Vanessa do Amaral Conrad Embrapa Beef Cattle Campo Grande Brazil Embrapa Western Agriculture Dourados Brazil Federal University of Grande Dourados (UFGD—Universidade Federal da Grande Dourados) Dourados Brazil

The aim of this study was to evaluate the physiological quality of Jatropha curcas L. seeds harvested at different stages of fruit maturation and two threshing methods. To analyze the mechanical damage rate an experiment was carried out with five stages of fruit maturation (green, yellow, ripe, dried on the ground and dried on the plant) threshed mechanically and manually. To evaluate the physiological quality, three tests were made;germination, vigor and electrical conductivity, all of them were applied in each storage (0, 120 and 240 days after harvest) and five stages of fruit maturation. It was feasible to infer that the maturation stage of yellow, ripe and dried on the plant fruits showed the best results of the physiological quality of the Jatropha curcas L. seeds. The manual threshing is also recommended in order to preserve the physiological quality of Jatropha curcas L. seeds. When the fruits were harvested at the appropriate stage of maturation (yellow, ripe and dry fruit on the plant) and threshing without mechanical damage, the Jatropha curcas L. seeds are possible to store for 240 days without loss in their germination. Regardless of the threshing system and the maturation stage, the seeds of Jatropha curcas L. lost their vigor from 120 days after harvested, when these seeds were stored in the shadow and in an environment without control on the relative humidity and air temperature.

关键词： Vigor Harvester Electrical Conductivity Germination

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Respiratory Muscle Strength Evaluation in Healthy Adult and Elderly Women—Respiratory Muscle Strength Assessment in Adult and Elderly

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Health 2018年第8期10卷 1031-1036页

作者： Maria Clara de souza Pereira Gama Maciel Cássio Daniel Araújo da Silva Fernanda Figueiroa Sanchez Jamilly Reboucas demosthenes Marques Pablo Costa Cortez Camila Miriam Suemi Sato barros do Amaral Naylla Morais de souza Roberta Lins Goncalves Federal University of Amazonas-UFAM ManausBrazil

Measurement of respiratory muscle strength is performed through static measurements of maximal respiratory pressures and is an important tool for determining the existence and prognosis of neuromuscular and pulmonary disorders, such as weakness or fatigue of this musculature. Objectives: To evaluate and compare respiratory muscle strength among healthy adult and elderly women. Methods: A total of 163 healthy women were recruited through convenience sampling at the family centers of the city of Manaus (AM, Brazil), divided into two groups: adult women (age 20 - 59 years) and elderly women (over 60 years of age). The evaluation was performed by Manovacuometry, in order to measure the maximum inspiratory pressure (MIP) and maximum expiratory pressure (MEP). For statistical analysis we used the Student’s T test, considering significant p value ≤ 0.05. Results: The final sample consisted of 116 adult women and 47 elderly women. The mean age in the group of women between 20 - 59 years was 44.5 ± 12 and the elderly group was 65 ± 6.6. MIP values in adult women were significantly higher when compared to the elderly (−108 ± 5.3 cmH2O × −79.5 ± 4 cmH2O, p = 0.0014, respectively). The same behavior was observed in the evaluation of MEP (87.1 ± 2.9 cmH2O in adults and 69.8 ± 3.5 cmH2O in the elderly;p = 0.0008). Conclusion: The results indicate that inspiratory and expiratory muscle strength is lower in older women, suggesting that the aging process tends to interfere with those muscles, resulting in altered respiratory system function.

关键词： Muscular Strength Evaluation Respiratory Muscles Aging

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Calibration of Hargreaves-Samani Equation for Estimating Reference Evapotranspiration in Sub-Humid Region of Brazil

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Journal of Water Resource and Protection 2013年第12期5卷 1-5页

作者： josé Romualdo de Sousa Lima Antonio Celso Dantas Antonino Eduardo Soares de souza Claude Hammecker Suzana Maria Gico Lima Montenegro Carlos Alberto Brayner de Oliveira Lira Academic Unity of Garanhuns Federal Rural University of Pernambuco Garanhuns Brazil Academic Unity of Serra Talhada Federal Rural University of Pernambuco Serra Talhada Brazil Land development department Office of Science for Land Development IRD Montpellier France Nuclear Energy department Federal University of Pernambuco Recife Brazil

Reference evapotranspiration (ETo) is an important parameter used in numerous applications, such as climatological and hydrological studies, as well as for water resources planning and management. There are several methods to estimate ETo, being that the FAO Penman-Monteith (EToPM) method is considered standard. This method needs many parameters (solar radiation, air temperature, humidity and wind speed), however there are still many uncovered areas, suggesting the need for methods of calculating evapotranspiration based on few meteorological elements, such as air temperature. Therefore, this study aimed to determine the ETo by Hargreaves-Samani method in the experimental watershed of the “Riacho do Papagaio” farm, in county of Sao Joao, in north-eastern Brazil, using data of 2011 and 2012. Reference evapotranspiration estimated by non-calibrated Hargreaves-Samani method (EToHS) was overestimated in all months (RMSE = 1.43 mm·d-1), mainly in months of lower evaporative demand (from May to July). Because of these tendencies, this method cannot be used in its original form to estimate ET for this region;therefore, a calibration of radiation adjustment coefficient (kRs) was performed. The calibrated Hargreaves-Samani method (EToHSc) had better performance (RMSE = 0.52 mm·d-1), being suitable for predicting ETo in this region.

关键词： Reference Evapotranspiration Penman-Monteith Hargreaves-Samani Air Temperature

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Synthesis and PAMPA Permeability Assay of New Sulfonyl Hydrazone derivatives

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Journal of Biosciences and Medicines 2019年第9期7卷 111-120页

作者： Luiz Felipe Schmitz de souza Tiago Tizziani Larissa Sens Dalila Venzke Inê s Maria Costa Brighente Moacir Geraldo Pizzolatti Ricardo josé Nunes Laborató rio Estrutura e Atividade Departamento de Qu&iacute mica LEAT-CFM-UFSC Universidade Federal de Santa Catarina Campus Trindade Florian&oacute polis SC Brazil Laborató rio de Quí mica de Produtos Naturais Departamento de Qu&iacute mica LQPN-CFM-UFSC Universidade Federal de Santa Catarina Campus Trindade Florian&oacute polis SC Brazil

This work describes the synthesis of seven new sulfonyl hydrazones, which were proposed from the general structure of acyl hydrazones and 2,4-dinitrosulfonamides. Sulfonyl hydrazones are a class of compounds known to exhibit a wide range of biological activity. In this sense, the study of the pharmacokinetic properties of the bioactive molecules is of extreme importance;therefore, in this article the permeability of these compounds with in vitro PAMPA assay mimetizing the permeability through the gastrointestinal tract as well as lipophilicity through miLogP was investigated. All compounds presented good permeability results;it was possible to make a structure-activity relationship with the obtained results and a comparison between the results.

关键词： Sulfonyl Hydrazones PAMPA Permeability Organic Synthesis Medicinal Chemistry

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Comparative Study on Cerebral Processing among Elderly People with Either Intellectual or Mechanical Work Activities, by Means of Electroencephalographic Techniques

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Journal of Behavioral and Brain Science 2020年第8期10卷 311-322页

作者： Arisson Euclides da Silva Leonardo Coelho de Mendonça Silva Lousane Leonoura Alves Santos Diêgo Lucas Ramos e Silva Paulo josé Medeiros de souza Costa Milton Vieira Costa josé Claudio da Silva Euclides Mauricio Trindade Filho State University of Health Sciences of Alagoas Biological Science Nucleus Maceió Brasil State University of Health Sciences of Alagoas Nucleus of Maternal Child and Adolescent Health Maceió Brasil

Background: The cognitive capacity is one of the determinants of quality of life in old age. In EEG, gamma rhythm represented electrical correlates of execution of cognitive activities. Objective: To compare the cortical areas activated during executive activities through the gamma rhythm (25 - 45 Hz) and the performance in the Frontal Assessment Battery (FAB) among older people who exercised intellectual and mechanical work during their lifetime. Methods: We compared the electroencephalographic record of the gamma rhythm potential (25 - 45 Hz) in the cortical quadrants during the execution of the FAB among 13 elderly people who exercised intellectual activity and 12 elderly people who performed mechanical work. Results: In the FAB, the intellectual group obtained a better performance with a mean score of 16.2/18, while the mechanical group obtained an average score of 14.9/18. Only a significant difference between groups was observed in the similarity test, with intellectual group leadership. There was no significant difference between groups in the overall analysis of gamma rhythm potential in the brain quadrants during the tests. Conclusion: Elderly people who performed intellectual activity performed better than the elderly who exercised mechanical work in the execution of the Frontal Evaluation Battery (FAB). There was a greater quantification of gamma rhythm in the group of elderly individuals who exercised intellectual activity, compared to those who exercised mechanical activity.

关键词： Electroencephalography Aged Gamma Rhythm

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