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HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium

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World Journal of Gastroenterology 2022年第29期28卷 3886-3902页

作者： Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López marcela salazar garcía Norma Velázquez-Guadarrama Laboratorio de Investigación en Enfermedades Infecciosas Hospital Infantil de México Federico GómezMexico City 06720Mexico Posgrado en Biomedicina y Biotecnología Molecular Laboratorio de Producción y Control de BiológicosEscuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalMexico City 11340Mexico Laboratorio de Adquisición de Hierro Universidad Autónoma de la Ciudad MéxicoPosgrado Ciencias GenómicaMexico City 03100Mexico Laboratorio Central de Microscopía Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalMexico City 11340Mexico Centro de estudios económicos y sociales en salud Hospital Infantil de México Federico GómezMexico City 06720Mexico Laboratorio de Investigación en Biología del Desarrollo y Teratogénesis Experimental Hospital Infantil de México Federico GómezMexico City 06720Mexico

BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence *** To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) *** Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional *** HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium *** technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS *** analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein

关键词： Hypothetical protein HP0953 Adherence Helicobacter pylori Glycocalyx Virulence factor Persistence

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Multi-temporal NDVI analysis using UAV images of tree crowns in a northern Mexican pine-oak forest

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林业研究:英文版 2023年第6期34卷 1855-1867页

作者： JoséLuis Gallardo-salazar marcela Rosas-Chavoya Marín Pompa-garcía Pablito Marcelo López-Serrano Emily garcía-Montiel Arnulfo Meléndez-Soto Sergio Iván Jiménez-Jiménez Instituto de Investigaciones Agropecuarias y Forestales Universidad Michoacana de San Nicolás de Hidalgo(UMSNH)58330 MoreliaMichoacánMexico Programa Institucional de Doctorado en Ciencias Agropecuarias y Forestales Universidad Juárez del Estado de DurangoRío Papaloapan y Blvd.DurangoValle del Sur s/n34120 DurangoDurangoMéxico Facultad de Ciencias Forestales Universidad Juárez del Estado de DurangoRío Papaloapan y Blvd.DurangoValle del Sur s/n34120 DurangoDurangoMéxico Instituto de Silvicultura e Industria de la Madera Universidad Juárez del Estado de DurangoBoulevard del Guadiana 501Ciudad UniversitariaTorre de Investigación34120 DurangoMéxico INIFAP-CENID RASPA Centro Nacional de Investigación Disciplinaria en Relación Agua-Suelo-Planta-Atmósfera Margen derecha canal Sacramento km 6.5Zona industrial35140 Gómez PalacioDurangoMexico

The use of unmanned aerial vehicles(UAV)for forest monitoring has grown significantly in recent years,providing information with high spatial resolution and temporal *** with multispectral sensors allow the use of indexes such as the normalized difference vegetation index(NDVI),which determines the vigor,physiological stress and photo synthetic activity of *** study aimed to analyze the spectral responses and variations of NDVI in tree crowns,as well as their correlation with climatic factors over the course of one *** study area encompassed a 1.6-ha site in Durango,Mexico,where Pinus cembroides,Pinus engelmannii,and Quercus grisea *** images were acquired with UAV and information on meteorological variables was obtained from NASA/POWER *** ANOVA explored possible differences in NDVI among the three *** correlation was performed to identify the linear relationship between NDVI and meteorological *** differences in NDVI values were found at the genus level(Pinus and Quercus),possibly related to the physiological features of the species and their *** grisea had the lowest NDVI values throughout the year which may be attributed to its sensitivity to relative humidity and *** the use of UAV with a multispectral sensor for NDVI monitoring allowed genera differentiation,in more complex forest analyses hyperspectral and LiDAR sensors should be integrated,as well other vegetation indexes be considered.

关键词： Multispectral images Normalized diff erence Vegetation index Phenology Unmanned aerial vehicles Multitemporal analysis

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Use of antiepileptic drugs in children with brain tumors: A review for acute management

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Journal of Acute Disease 2018年第5期7卷 181-185页

作者： Jennifer Herrera-Bejarano Amit Agrawal Claudia marcela Navas Valbuena Guru Dutta Satyarthee Chandramouli Balasubramanian Andrei F.Joaquim George Chater Cure Luis Rafael Moscote-salazar Physician Universidad del RosarioBogotaColombia Professor of Neurosurgery MM Institute of Medical Sciences&ResearchMaharishi Markandesh(w)ar UniversityMullana-Ambala133-207.HaryanaIndia Physician Universidad Industrial de SantanderUISBucaramangaColombia Assistant Professor of Neurosurgery All India Institute of Medical SciencesNew DelhiIndia Department of Neurosurgery Birmingham Children's HospitalBirminghamUK Department of Neurology Neurosurgery DivisionUniversity of Campinas(UNICAMP)Campinas-SPBrazil Neurosurgeon Clinica General del NorteBarranquillaColombia Neurosurgeon-Critical Care University of CartagenaCartagena de IndiasColombia

Patients with primary or secondary tumors in the central nervous system may have seizures resulting from direct tissue damage,metabolic abnormalities,infection,or toxic side effects of *** pediatric patients,it is more frequent to use drugs to control secondary *** this article,we discuss the main nuances of antiepileptic drugs for the proper management of children with central nervous system tumors.

关键词： Antiepileptic drugs Brain tumors Neuro oncology Seizures

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