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Aboveground nutrient components of eucalyptus camaldulensis and e. grandis in semiarid Brazil under the nature and the mycorrhizal inoculation conditions

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Journal of Forestry Research 2009年第1期20卷 15-22页

作者： marcela c. Pagano Antonio F. Bellote Maria R. scotti Microorganism-Plant Interaction LaboratolT Institute of Biological Sciences Federal University of Minas Gerais Av. Antonio Carlos 6627 Pampulha CEP： 31270-901 Belo Horizonte MG Brazil embrapa Forestry Estrada da Ribeira km 111 Caixa Postal 319 - Colombo PR - 83411-000- Brazil.

A study was conducted to evaluate the aboveground biomass, nutrient content and the percentages of mycorrhizal colonization in eucalyptus camaldulensis and eucalyptus grand＆ plantations in the semiarid region （ 15° 09′ s 43° 49′ W） in the north of the state of Minas Gerais in Brazil. Results show that the total above-ground biomass （dry matter） was 33.6 ***^-1 for e. camaldulensis and 53.1 Mg·ha^-1 for e. grandis. The biomass of the stem wood, leaves, branches, and stein bark for e. camaldulens accounted for 64.4%, 19.6%, 15.4%, and 0.6% of the total biomass, respectively （Table 2）; meanwhile a similar partition of the total above-ground biomass was also found for e. grand＆. The dry matter of leaves and branches of e. camaldulensis accounted for 35% of total biomass, and the contents of N, P, K, ca, Mg, and s in leaves and branches accounted for 15.5%, 0.7%, 12.3%, 22.6%, 1.9%, and 1.4% of those in total above-ground biomass, respectively. In the trunk （bark and wood）, nutrient accumulation in general was lower. Nutrient content of e. grand＆ presented little variation compared with that ore. camaJd^llensis. Wood localized in superior parts of trunk presented a higher concentration of P and bark contained significant amounts of nutrients, especially in e. grand＆. This indicated that leaving vegetal waste is of importance on the site in reducing the loss of tree productivity in this semi-arid region. The two species showed mycotrophy.

关键词： eucalyptus biomass nutrient components semi-arid region mycorrhizal symbioses Brazil

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changes in soil carbon stocks under Integrated crop-Livestock-Forest system in the Brazilian Amazon Region

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Agricultural sciences 2017年第9期8卷 904-913页

作者： marcela c.G.da conceicao eduardo s.Matos edison D.Bidone Renato de A.R.Rodrigues Renato c.cordeiro Universidade Federal Fluminense NiteróiBrazil embrapa Agrossilvipastoril SinopBrazil embrapa solos Rio de JaneiroBrazil

several studies indicate that the use of integrated production systems, such as integrated crop-livestock-forest systems (IcLF), improves the quality of the soil and consequently the sequestration of organic carbon in the soil. In this way, this work aims to evaluate the carbon stocks in soil under different management systems in the cerrado/Amazonia transition zone, namely: IcLF, no-tillage, pasture and eucalyptus plantation. For this, two soil samplings were done in 2011 and 2014, in the 0 - 5, 5 - 10 and 10 - 30 cm layers. soil carbon and nitrogen stocks were analyzed. IcLF system was the treatment that obtained the highest percentage of carbon gain (7.8%) after three years of establishment which represents to an increase of 5.5 Mg·ha-1. management systems, such as IcLF, with minimal soil disturbance combined with crop rotations that contribute to the quantity and quality of residues input, increase soil organic matter content. carbon stock data show the potential of IcLF systems to increase soil carbon stocks.

关键词： Agriculture soil carbon IcLF soil management

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聚乙二醇干扰素α联合水飞蓟宾胶囊治疗对慢性乙型肝炎患者肝功能的影响

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医师在线 2024年第8期14卷 48-51页

作者：白兴刚白满灵陈斌武威市人民医院肝病感染科甘肃武威733000

目的分析聚乙二醇干扰素α联合水飞蓟宾胶囊对慢性乙型肝炎患者肝功能的影响。方法选取我院2021年3月~2024年1月收治的84例慢性乙型肝炎患者,随机分为对照组和研究组,各42例。对照组采用聚乙二醇干扰素α治疗,研究组采用聚乙二醇干扰素... 详细信息

目的分析聚乙二醇干扰素α联合水飞蓟宾胶囊对慢性乙型肝炎患者肝功能的影响。方法选取我院2021年3月~2024年1月收治的84例慢性乙型肝炎患者,随机分为对照组和研究组,各42例。对照组采用聚乙二醇干扰素α治疗,研究组采用聚乙二醇干扰素α联合水飞蓟宾胶囊治疗。比较两组患者的临床疗效、炎症因子水平、肝功能、肝纤维化情况及不良反应发生情况。结果治疗前,两组白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、血清干扰素-γ(INF-γ)、转化生长因子-β(TGF-β)水平比较,差异无统计学意义(P>0.05);治疗后,研究组IL-6、TNF-α、TGF-β水平低于对照组,INF-γ水平高于对照组(PsT)、白蛋白(ALB)水平比较,差异无统计学意义(P>0.05);治疗后,研究组TBIL、ALT、AsT水平低于对照组,ALB水平高于对照组(Pha)、层黏连蛋白(LN)、Ⅳ型胶原(Ⅳc)比较,差异无统计学意义(P>0.05);治疗后,研究组ha、LN、Ⅳc水平低于对照组(P<0.05)。研究组治疗总有效率高于对照组(P<0.05),两组均未发生明显不良反应。结论聚乙二醇干扰素α联合水飞蓟宾胶囊治疗慢性乙型肝炎患者效果较好,且具有较高的安全性。

关键词：聚乙二醇干扰素α 水飞蓟宾胶囊慢性乙型肝炎肝功能

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Multi-temporal NDVI analysis using UAV images of tree crowns in a northern Mexican pine-oak forest

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林业研究:英文版 2023年第6期34卷 1855-1867页

作者： JoséLuis Gallardo-salazar marcela Rosas-chavoya Marín Pompa-García Pablito Marcelo López-serrano emily García-Montiel Arnulfo Meléndez-soto sergio Iván Jiménez-Jiménez Instituto de Investigaciones Agropecuarias y Forestales Universidad Michoacana de San Nicolás de Hidalgo(UMSNH)58330 MoreliaMichoacánMexico Programa Institucional de Doctorado en ciencias Agropecuarias y Forestales Universidad Juárez del Estado de DurangoRío Papaloapan y Blvd.DurangoValle del Sur s/n34120 DurangoDurangoMéxico Facultad de ciencias Forestales Universidad Juárez del Estado de DurangoRío Papaloapan y Blvd.DurangoValle del Sur s/n34120 DurangoDurangoMéxico Instituto de silvicultura e Industria de la Madera Universidad Juárez del Estado de DurangoBoulevard del Guadiana 501Ciudad UniversitariaTorre de Investigación34120 DurangoMéxico INIFAP-ceNID RAsPA centro Nacional de Investigación Disciplinaria en Relación Agua-suelo-Planta-Atmósfera Margen derecha canal Sacramento km 6.5Zona industrial35140 Gómez PalacioDurangoMexico

The use of unmanned aerial vehicles(UAV)for forest monitoring has grown significantly in recent years,providing information with high spatial resolution and temporal *** with multispectral sensors allow the use of indexes such as the normalized difference vegetation index(NDVI),which determines the vigor,physiological stress and photo synthetic activity of *** study aimed to analyze the spectral responses and variations of NDVI in tree crowns,as well as their correlation with climatic factors over the course of one *** study area encompassed a 1.6-ha site in Durango,Mexico,where Pinus cembroides,Pinus engelmannii,and Quercus grisea *** images were acquired with UAV and information on meteorological variables was obtained from NAsA/POWeR *** ANOVA explored possible differences in NDVI among the three *** correlation was performed to identify the linear relationship between NDVI and meteorological *** differences in NDVI values were found at the genus level(Pinus and Quercus),possibly related to the physiological features of the species and their *** grisea had the lowest NDVI values throughout the year which may be attributed to its sensitivity to relative humidity and *** the use of UAV with a multispectral sensor for NDVI monitoring allowed genera differentiation,in more complex forest analyses hyperspectral and LiDAR sensors should be integrated,as well other vegetation indexes be considered.

关键词： Multispectral images Normalized diff erence Vegetation index Phenology Unmanned aerial vehicles Multitemporal analysis

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Turbo-taxonomy to assemble a megadiverse lichen genus:seventy new species of cora(Basidiomycota:Agaricales:Hygrophoraceae),honouring David Leslie hawksworth’s seventieth birthday

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Fungal Diversity 2017年第3期84卷 139-207页

作者： Robert Lücking manuela Dal Forno Bibiana Moncada Luis Fernando coca Leidy Yasmín Vargas-Mendoza André Aptroot Laura Juliana Arias Betty Besal Frank Bungartz Diego Mauricio cabrera-Amaya marcela e.s.cáceres José Luis chaves sionara eliasaro Martha cecilia Gutiérrez Jesús e.Hernández Marin María de los Ángeles Herrera-campos María e.Holgado-Rojas harald Jonitz Martin Kukwa Fabiane Lucheta santiago Madriñán Marcelo Pinto Marcelli suzana Maria de Azevedo Martins Joel A.Mercado-Díaz Jorge Alberto Molina eduardo A.Morales Peter R.Nelson Freddy Nugra Francisco Ortega Telma Paredes Ayda Lucía Patiño Rouchi Nadine Peláez-Pulido Rosa emilia Pérez Pérez Gary B.Perlmutter eimy Rivas-Plata Javier Robayo camilo Rodríguez Diego Fernando simijaca edier soto-Medina Adriano Afonso spielmann Alejandra suárez-corredor Jean-Marc Torres carlos Alberto Vargas Alba Yánez-Ayabaca Gothamie Weerakoon Karina Wilk marcela celis Pacheco Mauricio Diazgranados Grischa Brokamp Thomas Borsch Patrick M.Gillevet Masoumeh sikaroodi James D.Lawrey Botanical Garden and Botanical Museum Berlin KöniginLuise-Straße 6-814195 BerlinGermany science education The Field Museum1400 South Lake ShoreChicagoIL 60605USA Department of Botany Smithsonian InstitutionNational Museum of Natural History10th St Constitution Ave NWWashingtonDC 20560USA Department of environmental science and Policy George Mason UniversityFairfaxVA 22030-4444USA Licenciatura en Biologı´a Universidad Distrital Francisco Jose´de CaldasCra.4 No.26D-54Torre de LaboratoriosHerbarioBogota´D.C.Colombia Herbario Universidad de caldas(FAUc) Edificio BicentenarioCalle 65No.26-10ManizalesA.A.275CaldasColombia ABL Herbarium G.v.d.Veenstraat 1073762 XK SoestThe Netherlands Biologı´a Universidad del Quindı´oCarrera 15 Calle 12 NorteArmeniaQuindı´oColombia 120 chavis Avenue LexingtonVA 24450USA charles Darwin Foundation Puerto AyoraSanta Cruz IslandGala´pagosEcuador schedestraße 8 53113 BonnGermany Jardı´n Bota´nico de Bogota´Jose´celestino Mutis Avenida Calle 63 No.68-95Bogota´D.C.Colombia Departamento de Biocieˆncias Universidade Federal de SergipeAvenida Vereador Olı´mpio Grandes/n.ItabaianaCEP:49.500-000 SergipeBrazil Laboratorio de Hongos Instituto Nacional de Biodiversidad(INBio)Apdo.22-3100Santo Domingo de HerediaCosta Rica Departamento de Botaˆnica Universidade Federal do Parana´Campus Polite´cnicoSetor de Cieˆncias Biolo´gicasAv.Cel.Francisco H.dos Santoss/nJardim das Ame´ricas81531-980 CuritibaPRBrazil Ingenierı´a Ambiental Universidad Distrital Francisco Jose´de CaldasCarrera 5 Este No.15-82Av.CircumvalarBogota´D.C.Colombia Instituto experimental Jardı´n Bota´nico Dr.Tobı´as Lasser Centro de Investigacio´n y DesarrolloUniversidad Central de VenezuelaAve.Salvador AllendeJardı´n Bota´nico de CaracasApartado#2156Caracas 1010-AVenezuela Departamento de Bota´nica Instituto de Biologı´aUniversidad Nacional Auto´noma de Me´xicoApartado Postal 70-36704510 Ciudad De Me´xicoMexico Facultad de ciencias Escuela Profesional de Biologı´aUniv

Following a large-scale phylogenetic study of the lichenized genus cora(Basidiomycota:Agaricales:Hygrophoraceae),we formally describe 70 new species,honouring the seventieth birthday of David Leslie hawksworth,one of the preeminent figures in mycology and lichenology in the past 50 *** on an updated phylogeny using the ITs fungal barcoding locus,we now recognize 189 taxa in a genus that until recently was considered to represent a single species;including this contribution,92 of these are formally recognized,including five taxa based on historical names or collections that have not been *** of cora can be recognized by a combination of morphological(size,colour,lobe configuration,surface hairs,hymenophore size and shape),anatomical(thallus thickness,cortex structure,photobiont type,hyphal papillae),and ecogeographical features(substrate,habitat,distribution),and a keytable allowing the identification of all accepted taxa is *** new species are:cora accipiter Moncada,Madrin˜a´n&Lücking ***.,*** Moncada,soto-Medina&Lücking ***.,*** Moncada,Dal Forno&Lücking ***.,*** Dal Forno,chaves&Lücking ***.,*** Moncada,***ı´guez&Lücking ***.,*** Lücking,Moncada&*** ***.,*** Moncada,Ya´nez-Ayabaca&Lücking ***.,*** Moncada,Patin˜o&Lücking ***.,*** Lücking,***&Dal Forno ***.,*** Holgado,Rivas Plata&Perlmutter ***.,*** Dal Forno,eliasaro&spielmann ***.,*** Nugra,Dal Forno&Lücking ***.,*** Lücking,Martins&Lucheta ***.,*** Moncada,R.-***´rez&Lücking ***.,*** Moncada,***.&Lücking ***.,*** Moncada,cabreraAmaya&Lücking ***.,*** Moncada,R.-***´rez&***.,*** Lücking,***&Dal Forno ***.,*** Moncada,***´rez-corredor&Lücking ***.,*** Moncada,***&Lücking ***.,***

关键词： Bolivia citizen science chile Mexico Puerto Rico sri Lanka

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基于RFH-NPT营养风险评估的饮食管理对慢性乙型肝炎肝硬化患者的影响

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护理实践与研究 2024年第9期21卷 1322-1329页

作者：马赵诣张炳勇杨嫚高远征时莉莉罗晓英河南省人民医院/郑州大学人民医院消化内科(河南省郑州市 450000)

目的探讨基于皇家自由医院-营养优先次序工具(RFH-NPT)营养风险评估的饮食管理对慢性乙型肝炎肝硬化患者营养状态及肝功能的影响。方法纳入2020年3月-2023年3月医院收治的120例慢性乙型肝炎肝硬化患者为研究对象,按照组间基本资料具... 详细信息

目的探讨基于皇家自由医院-营养优先次序工具(RFH-NPT)营养风险评估的饮食管理对慢性乙型肝炎肝硬化患者营养状态及肝功能的影响。方法纳入2020年3月-2023年3月医院收治的120例慢性乙型肝炎肝硬化患者为研究对象,按照组间基本资料具有可比性的原则分为对照组和观察组,各60例。对照组实施常规营养护理干预,观察组在对照组基础上实施基于RFH-NPT营养风险评估的饮食管理,两组均干预3个月。比较两组患者干预前后营养状态[体质指数(BMI)、上臂肌围(AMc)、白蛋白(ALB)、前白蛋白(PA)]、肝功能[丙氨酸转氨酶(ALT)、总胆红素(TBIL)、单胺氧化酶(MAO)]、肝纤维化程度[Ⅲ型前胶原(Pc-Ⅲ)、 Ⅳ型胶原( Ⅳ-c)、层粘连蛋白(LN)、透明质酸酶(ha)]、希望水平[Herth希望量表(HHI)]、生活质量[慢性肝病问卷(cLDQ)]。记录并比较两组患者干预期间并发症发生情况。结果干预后,观察组患者BMI、AMc、ALB、PA水平和HHI量表评分、cLDQ量表评分均高于对照组(Pc-Ⅲ、 Ⅳ-c、LN、ha水平比较差异无统计学意义(P>0.05)。干预期间,两组患者肝肾综合征、消化道出血、腹膜炎总发生率比较差异无统计学意义(P>0.05)。结论基于RFH-NPT营养风险评估的饮食管理应用于慢性乙型肝炎肝硬化患者中,可有效改善其营养状态和肝功能,缓解其肝纤维化进程,提高患者生活质量。

关键词：皇家自由医院-营养优先次序工具饮食管理慢性乙型肝炎肝硬化营养状态肝功能并发症

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Assessment of pathogens and risk factors associated with bloodstream infection in the year after pediatric liver transplantation

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World Journal of Gastroenterology 2022年第11期28卷 1159-1171页

作者： Yeong eun Kim Ho Jung choi Hye-Jin Lee Hyun Ju Oh Mi Kyoung Ahn seak Hee Oh Jung-man Namgoong Dae Yeon Kim Won Kyoung Jhang seong Jong Park Dong-Hwan Jung Deok Bog Moon Gi-Won song Gil-chun Park Tae-Yong ha chul-soo Ahn Ki-Hun Kim shin Hwang sung Gyu Lee Kyung Mo Kim Department of Pediatrics Asan Medical Center Children's HospitalUniversity of Ulsan College of MedicineSeoul 05505South Korea Division of Pediatric surgery Department of SurgeryAsan Medical Center Children's HospitalUniversity of Ulsan College of MedicineSeoul 05505South Korea Division of Hepatobiliary surgery and Liver Transplantation Department of SurgeryAsan Medical CenterUniversity of Ulsan College of MedicineSeoul 05505South Korea

BAcKGROUND Bloodstream infection(BsI)is one of the most significantly adverse events that can occur after liver transplantation(LT)in *** To analyze the profile of BsI according to the postoperative periods and assess the risk factors after pediatric *** clinical data,collected from medical charts of children(n=378)who underwent primary LT,were retrospectively *** primary outcome considered was BsI in the first year after *** and multivariate analyses were performed to identify risk factors for BsI and respective odds ratios(ORs).ResULTs Of the examined patients,106(28%)experienced 162 episodes of pathogen-confirmed BsI during the first year after *** were 1.53±0.95 episodes per children(mean±sD)among BsIcomplicated patients with a median onset of 0.4 mo *** most common pathogenic organisms identified were coagulase-negative staphylococci,followed by enterococcus *** streptococcus *** half(53%)of the BsIs were of unknown *** analysis demonstrated that young age(≤1.3 year;OR=2.1,P=0.011),growth failure(OR=2.1,P=0.045),liver support system(OR=4.2,P=0.008),and hospital stay of>44 d(OR=2.3,P=0.002)were independently associated with BsI in the year after *** BsI was frequently observed in patients after pediatric LT,affecting survival *** profile of BsI may inform clinical treatment and management in high-risk children after LT.

关键词： Bloodstream infection Liver transplantation children Pathogens

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Assessing the Impact of sulfur Atmospheric Deposition on Terrestrial ecosystems close to an Industrial corridor in the southeast of Mexico

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Journal of environmental Protection 2017年第10期8卷 1158-1177页

作者： Rosa M. cerón Julia G. cerón manuel Muriel marcela Rangel Reyna del c. Lara Beatriz Tejero Martha P. Uc Abril Rodríguez chemistry Faculty Autonomous University of Carmen Ciudad del Carmen Campeche Mexico Mexican Institute of Petroleum Marine Zone Ciudad del Carmen Campeche Mexico sciences of Health Faculty Autonomous University of Carmen Ciudad del Carmen Campeche Mexico center of environmental sciences Research Autonomous University of Carmen Ciudad del Carmen Campeche Mexico

The main objective of this research work was to diagnose the vulnerability of terrestrial ecosystems to s deposition in Atasta region in campeche state, Mexico, comprising two simultaneous sampling programs in both, soil and atmospheric deposition on an annual basis during three climatic periods: dry, rainy and cold fronts seasons. From the estimation of soil properties estimation (pH, texture, mineralogy, cationic exchange capacity, and basis saturation %), critical loads and sensitivity classes were assigned to sampled soils based according to the empirical methodology proposed by UNece. During the dry season, 10 sites fell into sensitivity class 2 (moderately sensitive) and 3 (sensitive). On the other hand, during the rainy season, 8 sites showed a sensitivity class 1 (highly sensitive) and 2 sites presented a sensitivity class 2 (moderately sensitive);whereas along cold fronts season, 12 sites fell into sensitivity class 1 that corresponds to highly sensitive. sensitivity classes showed a seasonal trend, with a higher sensitivity during rainy and cold fronts seasons;this agrees with the kind of sources influencing on the study area as a result of the prevailing meteorology during these climatic periods. Likewise, s concentration in atmospheric deposition was determined by turbidimetric method, and s deposition fluxes were estimated from surface area of the funnel opening of the sampling device and the sampling period. s deposition fluxes ranged from 0.29 and 14.06 kg s ha-1·yr-1;with a mean value of 8.57 kg s ha-1·yr-1. From the comparison between the current deposition rates and proposed critical loads, exceedances percentages were obtained (from 1.65% to 62.8%) and mapped to identify critical zones of s deposition in the studied area. It was established the important role which mangrove vegetation plays in the attenuation of the potential ecological effects on terrestrial ecosystems of the study area associated to atmospheric deposition.

关键词： Atmospheric Deposition critical Loads sulfur campeche Mexico

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紫色土丘陵坡地5种土地利用方式土壤腐殖物质特征比较

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草地学报 2021年第10期29卷 2303-2308页

作者：杨满元杨宁湖南环境生物职业技术学院园林学院湖南衡阳421005

以衡阳紫色土的天然草地(Natural grassland,NG)、人工草地(Tame grassland,TG)、灌草地(Frutex and grassland,FG)、灌丛地(Frutex,FX)和乔灌地(Arbor and frutex,AF)5种土地利用方式的土壤为研究对象,分析5种土地利用方式土壤的腐殖物质(Humic substance,Hs)组成和性质,并探讨与土壤理化性质之间的关系。研究发现:FG的腐殖物质含量最高,其次为TG,NG最低;土壤腐殖物质总碳(Total carbon,T-c)以胡敏素碳(Humin carbon,Hu-c)含量最高,胡敏酸碳(Humic acid carbon,ha-c)含量最低;TG与FG的胡敏酸碳/富里酸碳[Humic acid carbon/Fulvic acid carbon,(ha-c)/(FA-c)]含量最高,NG最低,TG,FG,FX与AF 0~20 cm土层的(ha-c)/(FA-c)含量显著高于20~40 cm土层(Ps,ha-c,富里酸碳,Hu-c,土壤有机碳(soil organic carbon,sOc),碱解氮(Alkali-hydrolyzable nitrogen,AN)与速效磷(Available phosphorus,AP)两两间呈显著或极显著正相关关系(Ps,ha-c,FA-c,Hu-c,sOc,AN,AP呈显著负相关关系(Ps组分含量较低,土质较差,而TG,FG的Hs含量较高,土质较好,建议将NG开发成TG与FG土地利用方式,以提高土壤肥力。

关键词：腐殖物质胡敏酸碳富里酸碳胡敏素碳土地利用方式

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TLR2 and TLR4 polymorphisms influence m RNA and protein expression in colorectal cancer

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World Journal of Gastroenterology 2015年第25期21卷 7730-7741页

作者： marcela Alcantara Proenca Juliana Garcia de Oliveira Aline cristina Targa cadamuro Maysa succi Joao Gomes Netinho eny Maria Goloni-Bertolo érika cristina Pavarino Ana elizabete silva Department of Biology UNESPSao Paulo State University Usc-sacred Heart University PróReitoria de Pesquisa e Pós Graduacao Department of surgery School of MedicineFAMERPSao Josédo Rio Preto 15090-000SPBrazil UPGeM School of MedicineFAMERPSao Josédo Rio Preto 15090-000SPBrazil

AIM: To evaluate the effect of promoter region polymorphisms of toll-like receptor(TLR)2-196 to-174 del and TLR4-1607T/c(rs10759932) on m RNA and protein expression in tumor tissue and of TLR4+896A/G(rs4986790) on colorectal cancer(cRc) ***: The TLR2-196 to-174 del polymorphism was investigated using allele-specific polymerase chain reaction(PcR) and the TLR4-1607T/c and TLR4+896A/G by PcR-restriction fragment length p o l y m o r p h i s m( R F L P). W e g e n o t y p e d 4 3 4 D N A samples from 194 cRc patients and 240 healthy individuals. The m RNA relative quantification(RQ) was performed in 40 tumor tissue samples by quantitative PcR Taq man assay, using specific probes for TLR2 and TLR4 genes, and AcTB and GAPDH reference geneswere used as endogenous controls. Protein expression was analyzed by immunohistochemistry with specific primary ***: No association was found for TLR4-1607T/c and TLR4+896A/G by three statistical models(logadditive, dominant and recessive). However, based on dominant and log-additive models, the polymorphic variant TLR2-196 to-174 del was associated with increased cRc risk [dominant: odds ratio(OR) = 1.72, 95%cI: 1.03-2.89; P = 0.038 and log-additive: OR =1.59, 95%cI: 1.02-2.48; P = 0.039]. TLR2 m RNA expression was increased in tumor tissue(RQ = 2.36) when compared to adjacent normal tissue(RQ = 1; P ereas the TLR4 m RNA showed a basal expression(RQ = 0.74 vs RQ = 1, P = 0.452). Immunohistochemistry analysis of TLR2 and TLR4 protein expression was concordant with the findings of m RNA expression. In addition, the TLR2-196 to-174 del variant carriers showed m RNA relative expression 2.19 times higher than wild-genotype carriers. The TLR2 protein expression was also higher for the TLR2-196 to-174 del variant carriers [117 ± 10 arbitrary unit(a.u.) vs 95 ± 4 a.u., P = 0.03]. However, for the TLR4-1607T/c polymorphism no significant difference was found for both m RNA(P = 0.56) and protein expression(P

关键词： Toll-like receptor 2 Toll-like receptor 4 colorectal cancer Protein expression Gene expression Genetic polymorphisms

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