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Fluorescence Intensity Decay Shape Analysis Microscopy （FIDSAM） for Quantitative and Sensitive Live-Cell Imaging： A Novel technique for Fluorescence Microscopy of Endogenously Expressed Fusion-Proteins

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Molecular Plant 2010年第3期3卷 555-562页

作者： Frank Schleifenbaum Kirstin Elgass Marcus Sackrow Katharina Caesar Kenneth Berendzen Alfred j. Meixner Klaus Hatter Center for Plant Molecular Biology Department of Plant Physiology University of Tubingen Auf der Morgenstelle 1 72076 Tubingen Germany Institute of Physical and theoretical Chemistry University of Tubingen Auf der Morgenstelle 8 72076 Tubingen Germany

Fluorescent studies of living plant cells such as confocal microscopy and fluorescence lifetime imaging often suffer from a strong autofluorescent background contribution that significantly reduces the dynamic image contrast and the quantitative access to sub-cellular processes at high spatial resolution. Here, we present a novel technique--fluorescence intensity decay shape analysis microscopy （FIDSAM） to enhance the dynamic contrast of a fluorescence image of at least one order of magnitude. the method is based on the analysis of the shape of the fluorescence intensity decay （fluorescence lifetime curve） and benefits from the fact that the decay patterns of typical fluorescence label dyes strongly differ from emission decay curves of autofluorescent sample areas. Using FIDSAM, we investigated Arabidopsis thaliana hypocotyl cells in their tissue environment, which accumulate an eGFP fusion of the plasma membrane marker protein LtI6b （LtI6b-eGFP） to low level. Whereas in conventional confocal fluorescence images, the membranes of neighboring cells can hardly be optically resolved due to the strong autofluorescence of the cell wall, FIDSAM allows for imaging of single, isolated membranes at high spatial resolution. thus, FIDSAM will enable the sub-cellular analysis of even low-expressed fluorophoretagged proteins in living plant cells. Furthermore, the combination of FIDSAM with fluorescence lifetime imaging provides the basis to study the local physico-chemical environment of fluorophore-tagged biomolecules in living plant cells.

关键词： Cell structure cell walls membrane proteins high-resolution fluorescence microscopy.

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Proteome-Based Clustering Approaches Reveal Phylogenetic Insights into Amphistegina

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journal of Earth Science 2022年第6期33卷 1469-1479页

作者： Marleen Stuhr Bernhard Blank-Landeshammer Achim Meyer Vera Baumeister jörg Rahnenführer Albert Sickmann Hildegard Westphal Leibniz Centre for tropical Marine Research(ZMt) BremenGermany Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V. DortmundGermany Austrian Competence Center for Feed and Food Quality Safety and InnovationWelsAustria Chrestos Concept GmbH&Co KG EssenGermany Department of Statistics TU Dortmund UniversityDortmundGermany Medizinisches Proteom-Center(MPC) Ruhr-Universität BochumBochumGermany Department of Chemistry University of AberdeenOld AberdeenScotlandUK Department of Geosciences Bremen UniversityBremenGermany King Abdullah University of Science and technology(KAUSt) ThuwalSaudi Arabia

Foraminifera are highly diverse and have a long evolutionary *** key bioindicators,their phylogenetic schemes are of great importance for paleogeographic applications,but may be hard to recognize *** phylogenetic relationships within the prominent genus Amphistegina are still *** studies on Amphistegina have so far only focused on genetic diversity within single species and suggested a cryptic diversity that demands for further *** molecular sequencing-based approaches,different mass spectrometry-based proteomics approaches are increasingly used to give insights into the relationship between samples and organisms,especially as these do not require reference *** better understand the relationship of amphisteginids and test different proteomics-based approaches we applied de novo peptide sequencing and similarity clustering to several populations of Amphistegina lobifera,*** and *** also analyzed the dominant photosymbiont community to study their influence on holobiont *** analyses indicate that especially de novo peptide sequencing allows to reconstruct the relationship among foraminiferal holobionts,although the detected separation of *** from *** and *** may be partly influenced by their different photosymbiont *** resulting dendrograms reflect the separation in two lineages previously suggested and provide a basis for future studies.

关键词： large benthic foraminifera de novo peptide sequencing tandem mass spectra clustering LC-MS/MS runs proteomics symbiont diversity phylogeography Fragilariales

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tWO LAtERALLY BRANCHED GRAPtOLItES

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古生物学报 1953年第4期 197-200页

作者： A.t.MU Institute of Palaeontology,Academia Sinica

It is known that the branching of the graptolite stipes falls into two types,the dichotomous and the *** the former type the two branches split offsimultaneously and symmetrically with equal magnitude in position;whereas inthe latter type the main stipes and the lateral ones differ *** branching of all the primitive forms of the true graptolites sbelong tothe dichotomous *** of laterally Branching stipes began to becomeabundant in the late Lower Ordovician *** seems probable that the lateral-ly branching forms were derived from the dichotomous *** isa genus representing the transitional form between the two types,as is shownin textfignre 1(p.92).

关键词： tWO LAtERALLY BRANCHED GRAPtOLItES

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官僚机构与民主责任与绩效

官僚机构与民主责任与绩效

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1000年

作者：（美）小威廉·t·格姆雷（美）斯蒂芬·j·巴拉俞沂暄译

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EXPERIENCE WItH tHE PROVIEW EYE PRESSURE MONItOR

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国际眼科杂志 2003年第1期3卷 48-48页

作者： j.t.Wilensky University of Illinois at Chicago IL USA

Objective: to compare IOP readings obtained by Goldmann ap-planation tonometry with those obtained with the ProView Monitor Methods: 34 patients with open angle glaucoma or ocular hypertension were taught to use the ProView tonometer. then their IOP was measured three times with the Goldmann tonometer and then three times with the ProView tonometer. In 14 of the pa-

关键词：青光眼眼压监测 IOP值测量准确性

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Regeneration of Nothofagus dombeyi(Mirb.)Ø*** little to moderately disturbed southern beech forests in the Andes of Patagonia(Argentina)

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Forest Ecosystems 2023年第4期10卷 472-482页

作者： Stefan Zerbe Stefanie t.Storz Georg Leitinger Natalia Zoe joelson joséBava Steffi Heinrichs Christoph Leuschner Gabriel Loguercio Alois Simon María F.Urretavizcaya Helge Walentowski Faculty of Agricultural Environmental and Food SciencesFree University of Bozen-BolzanoBolzanoItaly Department of Ecology University of InnsbruckInnsbruckAustria Faculty of Resource Management HAWK University of Applied Sciences and ArtsGöttingenGermany Department of Plant Ecology and Ecosystem Research Albrecht-von-Haller-Institute for Plant SciencesUniversity of GöttingenGöttingenGermany Centro de Investigación y Extensión Forestal Andino Patagónico(CIEFAP) EsquelArgentina Department of Forest Planning Tyrol Forest AdministrationOffice of the Tyrolean GovernmentInnsbruckAustria

Natural forests and stands subjected to little to moderate human impact are continuously declining worldwide and with these,their biodiversity and ecosystem *** Nothofagus forests in the south of the South American continent are in a pristine state or only moderately impacted by *** grazing by livestock,in the past and still today often practiced in a non-sustainable way is,however,increasingly under discussion to meet current environmental and socio-economic ***,we investigate the regeneration of Nothofagus dombeyi,a keystone species of the Patagonian Andes,in secondary forests in Argentinian northern Patagonia,particularly addressing the role of disturbance through grazing by livestock at various *** test the hypothesis that the regeneration of this tree species is favored by grazing impact and,thus,disturbance of the herb layer and soil *** support of our hypothesis,Nothofagus dombeyi regeneration was significantly higher in terms of individuals and height classes in moderately grazed *** analysis shows significant positive effects of moderate grazing pressure,herb layer cover,and the occurrence of bare soil on the regeneration of Nothofagus *** results show that an integration of livestock grazing and forest regeneration is possible and that agroforestry systems can be an adequate management option for stakeholders in the region.A grazing management can also be part of a forest fire prevention ***,the regeneration success and grazing pressure should be continuously monitored.

关键词： Agroforestry Disturbance Fire management Forest grazing Forest management Plant traits

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基于小波变换的彩色自然图像数据库自动检索

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红外与毫米波学报 2000年第1期19卷 29-32页

作者：刘建峰李春茂 N.t.thao 戚飞虎美国朗讯科技有限公司贝尔实验室上海研究中心上海200030 香港科技大学计算机系香港科技大学电子与电机工程系上海交通大学计算机系上海200030

针对彩色自然图像数据库,提出了一种新的基于小波帧包变换的自动检索方法。该方法有效结合了纹理信息和颜色信息,克服了以往方法将低层特征孤立起来的缺陷,同时,图像检索过程以渐进的方式进行.本文方法无论在检索效率及检索准确率上都... 详细信息

针对彩色自然图像数据库,提出了一种新的基于小波帧包变换的自动检索方法。该方法有效结合了纹理信息和颜色信息,克服了以往方法将低层特征孤立起来的缺陷,同时,图像检索过程以渐进的方式进行.本文方法无论在检索效率及检索准确率上都能获得好的效果.

关键词：纹理小波变换彩色自然图像数据库自动检索

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Bio-Fertilizer,Ground Magnesium Limestone and Basalt Applications May Improve Chemical Properties of Malaysian Acid Sulfate Soils and Rice Growth

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Pedosphere 2014年第6期24卷 827-835页

作者： Q.A.PANHWAR U.A.NAHER O.RADZIAH j.SHAMSHUDDIN I.MOHD RAZI Department of Land Management Faculty of AgricultureUniversity Putra MalaysiaSerdang 43400 (Malaysia) Institute of tropical Agriculture University Putra MalaysiaSerdang 43400 (Malaysia) Soil Science Division Bangladesh Rice Research InstituteGazipur 1701 (Bangladesh)

Acid sulfate soils are normally not suitable for crop production unless they are appropriately ameliorated. An experiment was conducted in a glasshouse to enhance the growth of rice, variety MR219, planted on an acid sulfate soil using various soil *** soil was collected from Semerak, Kelantan, Malaysia. Ground magnesium limestone(GML), bio-fertilizer, and basalt(each 4t ha-1) were added either alone or in combinations into the soil in pots 15 d before transplanting. Nitrogen, P and potash were applied at 150, 30, and 60 kg ha-1, respectively. three seven-day-old rice seedlings were transplanted into each pot. the soil had a p H of 3.8 and contained organic C of 21 g kg-1, N of 1.2 g kg-1, available P of 192 mg kg-1, exchangeable K of 0.05 cmol c kg-1,and exchangeable Al of 4.30 cmol c kg-1, with low amounts of exchangeable Ca and Mg(0.60 and 0.70 cmol c kg-1). Bio-fertilizer treatment in combination with GML resulted in the highest p H of 5.4. the presence of high Al or Fe concentrations in the control soil without amendment severely affected the growth of rice. At 60 d of growth, higher plant heights, tiller numbers and leaf chlorophyll contents were obtained when the bio-fertilizer was applied individually or in combination with GML compared to the control. the presence of beneficial bacteria in bio-fertilizer might produce phytohormones and organic acids that could enhance plant growth and subsequently increase nutrient uptake by rice. Hence, it can be concluded that addition of bio-fertilizer and GML improved rice growth by increasing soil p H which consequently eliminated Al and/or Fe toxicity prevalent in the acid sulfate soil.

关键词： Al toxicity amendments beneficial bacteria Fe toxicity indoleacetic acid organic acids

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LOW tEMPERAtURE tHERMODYNAMIC StUDY OF tHE StABLE AND MEtAStABLE PHASES OF SILVER

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Acta Metallurgica Sinica(English Letters) 2007年第5期20卷 355-362页

作者： X.B. Li Y.Q. Xie Y.Z. Nie H.j. Peng Research Center of Advanced Materials Xiangtan University Xiangtan 411105 China School of Material Science and Engineering University Changsha 410083 China Central South

the low temperature thermodynamics of the stable phase of silver was assessed by the polynomial model and Debye model from the experimental data available in the literature. By means of the constrained non-linear least squares curve fitting arithmetic, two sets of parameter values were determined. the expression of the thermodynamic functions cp（t） and G（t）-H （298.15K） at 0-298.15K were presented. Only the low temperature thermodynamics of the metastable phase of silver can be extrapolated by the Debye model the expression of the thermodynamic functions cp（t） at 0- 298.15K was presented.

关键词： silver thermodynamic heat capacity SGtE (Scientific Group thermodata Europe)

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CAR t-cell immunotherapy: a powerful weapon for fighting hematological B-cell malignancies

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Frontiers of Medicine 2021年第6期15卷 783-804页

作者： jian-Qing Mi jie Xu jianfeng Zhou Weili Zhao Zhu Chen j.joseph Melenhorst Saijuan Chen State Key Laboratory of Medical Genomics Shanghai Institute of HaematologyNational Research Centre for Translational MedicineRuijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai200025China Department of Pathology&Laboratory Medicine University of PennsylvaniaPhiladelphiaPA19104USA Immunotherapy Research Centre for Hematologic Diseases of Hubei Province Department of HaematologyTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

the current standard of care in hematological malignancies has brought considerable clinical benefits to ***,important bottlenecks still limit optimal achievements following a current medical *** genetic complexity of the diseases and the heterogeneity of tumor clones cause difficulty in ensuring long-term efficacy of conventional treatments for most hematological ***,new treatment strategies are necessary to improve clinical *** antigen receptor t-cell(CAR t)immunotherapy opens a new path for targeted therapy of hematological *** this review,through a representative case study,we summarize the current experience of CAR t-cell therapy,the management of common side effects,the causative mechanisms of therapy resistance,and new strategies to improve the efficacy of CAR t-cell therapy.

关键词： CAR t cells hematological malignancies review

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