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First genetic linkage map of Lathyrus cicera based on RNA sequencing-derived markers: Key tool for genetic mapping of disease resistance

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Horticulture Research 2018年第1期5卷 345-358页

作者： Carmen Santos Nuno Felipe Almeida mara lisa alves Ralf Horres Nicolas Krezdorn Susana Trindade Leitão Thaïs Aznar-Fernández Björn Rotter Peter Winter Diego Rubiales Maria Carlota Vaz Patto Instituto de Tecnologia Química e Biológica António Xavier Universidade Nova de LisboaAv.da RepúblicaOeiras2780-157Portugal GenXPro GmbH Frankfurt am MainD-60438Germany Institute for Sustainable Agriculture CSICCórdobaE-14004Spain

The Lathyrus cicera transcriptome was analysed in response to rust(Uromyces pisi)infection to develop novel molecular breeding tools with potential for genetic mapping of resistance in this robust orphan legume *** RNA-seq library each was generated from control and rust-inoculated leaves from two *** genotypes with contrasting quantitative resistance,de novo assembled into contigs and sequence polymorphisms were *** toto,19,224 SNPs differentiate the susceptible from the partially resistant genotype’s *** addition,we developed and tested 341 expressed E-SSR markers from the contigs,of which 60.7%varied between the two *** genotypes.A first *** linkage map was created using part of the developed markers in a RIL population from the cross of the two *** map contains 307 markers,covered 724.2 cM and is organised in 7 major and 2 minor linkage groups,with an average mapping interval of 2.4 *** genic markers also enabled us to compare their position in *** map with the physical position of the same markers mapped on Medicago truncatula genome,highlighting a high macrosyntenic conservation between both *** study provides a large new set of genic polymorphic molecular markers with potential for mapping rust *** represents the first step towards genomics-assisted precision breeding in ***.

关键词： breeding linkage resistance

Droplet digital microfluidic system for screening filamentous fungi based on enzymatic activity

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Microsystems & Nanoengineering 2022年第6期8卷 97-112页

作者： Kenza Samlali Chiara Leal alves mara Jezernik Steve C.C.Shih Department of Electrical and Computer Engineering Concordia UniversityMontréalQCCanada Centre for Applied Synthetic Biology Concordia UniversityMontréalQCCanada Department of Chemical Engineering and Applied Chemistry University of TorontoTorontoONCanada Department of Biology Concordia UniversityMontréalQCCanada

Fungal cell-wall-degrading enzymes have great utility in the agricultural and food *** cell-wall-degrading enzymes are known to have functions that can help defend against pathogenic *** existing methods used to discover these enzymes are not well adapted to fungi culture and morphology,which prevents the proper evaluation of these *** report the first droplet-based microfluidic method capable of long-term incubation and low-voltage conditions to sort filamentous fungi inside nanoliter-sized *** new method was characterized and validated in solid-phase media based on colloidal chitin such that the incubation of single spores in droplets was possible over multiple days(2–4 days)and could be sorted without droplet *** long-term culture,we examined the activity of cell-wall-degrading enzymes produced by fungi during solid-state droplet fermentation using three highly sensitive fluorescein-based *** also used the low-voltage droplet sorter to select clones with highly active cell-wall-degrading enzymes,such as chitinases,β-glucanases,and β-Nacetylgalactosaminidases,from a filamentous fungi droplet library that had been incubated for>4 *** new system is portable,affordable for any laboratory,and user-friendly compared to classical droplet-based microfluidic *** propose that this system will be useful for the growing number of scientists interested in fungal microbiology who are seeking high-throughput methods to incubate and sort a large library of fungal cells.

关键词： system fluid culture

Uncovering the uncertainty: Risk factors and clinical relevance of P1 lesions on small bowel capsule endoscopy of anemic patients

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World Journal of Gastroenterology 2016年第38期22卷 8568-8575页

作者： Tiago Cúrdia Gon?alves mara Barbosa Bruno Rosa Maria Jo?o Moreira José Cotter Gastroenterology Department Hospital da Senhora da Oliveira School of Health Sciences University of Minho ICVS/3B’s PT Government Associate Laboratory

AIM To identify risk factors for P1 lesions on small bowel capsule endoscopy(SBCE) and to describe the natural history of anemic patients with such type of *** One hundred patients were consecutively selected for a case-control analysis performed between 37 cases with P1 lesions and 63 controls with negative SBCE. Age, gender, comorbidities and regular medication were collected. Rebleeding, further investigational studies and death were also analyzed during the *** No significant differences on gender, median age or Charlson index were found between groups. Although no differences were found on the use of proton pump inhibitors, acetylsalicylic acid, anticoagulants or antiplatelet agents, the use of non-steroidal antiinflammatory drugs(NSAID) was associated with a higher risk of P1 lesions(OR = 12.00, 95%CI: 1.38-104.1). From the 87 patients followed at our center, 39 were submitted to additional studies for investigation of iron-deficiency anemia(IDA), and this was significantly more common in those patients with no findings on SBCE(53.7% vs 30.3%, P = 0.033). A total of 29 patients had at least one rebleeding or IDA recurrence episode and 9 patients died of non-anemia related causes but no differences were found between cases and *** P1 lesions are commonly found in patients with IDA submitted to SBCE. The use of NSAID seems to be a risk factor for P1 lesions. The outcomes of patients with P1 lesions do not differ significantly from those with P0 lesions or normal SBCE.

关键词： P1 lesions Iron-deficiency anemia Small bowel capsule endoscopy Risk factors Rebleeding

The ARQUIGRAFIA project: A Web Collaborative for Review Only Environment for Architecture and Urban Heritage Image

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Journal of Data and Information Science 2020年第1期5卷 51-67页

作者： Vânia mara alves Lima Ci bele Araújo Camargo Marques dos Santos Artur Simões Rozestraten Department of Information and Culture University of São PauloSão PauloBrazil Department of Architecture Technology University of São PauloSão PauloBrazil

Purpose:This paper presents the ARQUIGRAFIA project,an open,public and nonprofit,continuous growth web collaborative environment dedicated to Brazilian architectural photographic ***/methodology/approach:The ARQUIGRAFIA project promotes the active and collaborative participation among its institutional users(GLAMs,NGOs,laboratories and research groups)and private users(students,professionals,professors,researchers),both can create an account and share their digitized iconographic collections in the same Web environment by uploading their files,indexing,georeferencing and assigning a Creative Commons ***:The development of users interactions by means of semantic differentials impressions recording on visible plastic-spatial aspects of the architectures in synthetic infographics,as well as by the retrieval of images through an advanced system search based on those impressions *** gamification means,the system often invites users to review images’in order to improve images’data *** pilot project named Open Air Museum that allows users to add audio descriptions to images in *** interface for users’digital curatorship will be soon *** limitations:The ARQUIGRAFIA’s multidisciplinary team gathering professorsresearchers,graduate and undergraduate students from the Architecture and Urbanism,Design,Information Science,Computer Science faculties of the University of S?o Paulo,demands continuous financial resources for grants,for contracting third party services,for the participation in scientific events in Brazil and abroad,and for *** 2016,significant budget cuts in the University of S?o Paulo own research funds and in Brazilian federal scientific agencies can compromise the continuity of this *** implications:The open source template called+GRAFIA that can freely help other areas of knowledge to build their own visual Web collaborative ***/value:The collaborative natu

关键词： Metadata Architectural images Collaborative web environment Digital repository

The Importance of Three-Dimensional Imaging from CBCT in Elucidating a Well-Defined Radiolucency Image within the Maxillary Sinus: A Case Report

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Open Journal of Radiology 2014年第3期4卷 255-259页

作者： Ricardo alves de Mesquita Cláudia Borges Brasileiro Tania mara Pimenta Amaral Department of Oral Surgery and Pathology School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Brazil lDepartment of Oral Surgery and Pathology School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Brazi

This study describes a case of antral septum with alveolar process extension that is identified using cone-beam computed tomography (CBCT). Periapical radiolucency was observed in the maxillary sinus, and clinical and radiographic examinations ruled out the possibility of odontogenic lesions. CBCT was performed to elucidate the radiolucency identified using periapical radiography. A 3-D image indicated that the maxillary sinus extended into the alveolar process toward the palatal cortical bone in the region of the maxillary right first molar, as well as an antral septum extending from the inferior and lateral wall of the right maxillary sinus. CBCT is an important tool for use in dental practice because CBCT images reveal the entire volume of the maxillary sinus and allow for identification of patient anatomy and anatomical variations, which is essential for planning appropriate surgical interventions.

关键词： Diagnostic Imaging Cone-Beam Computed Tomography Radiography Maxillary Sinus

Comparison between Elderly Persons of the Urban Community, According to the Indicative of Depression

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Open Journal of Nursing 2017年第2期7卷 158-168页

作者： Lilane Maria alves Silva Sheron Hellen da Silva Darlene mara dos Santos Tavares Leiner Resende Rodrigues The Federal University of Triângulo Mineiro Uberaba MG Brazil

Objectives: To compare the sociodemographic and economic profile, functional capacity, number of self-referred morbidities and level of physical activity among the elderly with and without indicative of depression. Methods: Cross-sectional and analytical study, with 980 elderly persons (250 with and 730 without indicative of depression) residing in the city of Uberaba, in the State of Minas Gerais, Brazil, between August 2012 and May 2013. We used the BOMFAQ Questionnaire, GDS 15, Katz Index, Lawton and Brody Scale and IPAQ. For data analysis, descriptive statistics and the Chi-square test (p Results: In the comparison between the groups, there were significant differences regarding the female gender (p = 0.009), education (p = 0.008), individual income (p Conclusion: The comparison of the existence or not of depressive symptoms among the elderly enables a more effective planning of public policies aimed at health promotion and adequate treatment in cases of disease already present and the subsidization of preventive strategies, using data such as risk factors and associated factors and ways to research depression.

关键词： Depression Aged Motor Activity Activities of Daily Living

Formulations Containing Curcumin or Trans-Resveratrol Increase Dermal Thickness in Rats Submitted to Chemical Peeling

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Journal of Cosmetics, Dermatological Sciences and Applications 2017年第1期7卷 14-26页

作者： Gisele mara Silva Gonç alves Pedro Paulo Barros Gustavo Henrique da Silva Erica Mendes dos Santos Amanda Figlia Minutti Faculty of Pharmaceutical Sciences Pontifical Catholic University of Campinas Campinas Brazil

Plant-derived substances such as curcumin and trans-resveratrol, both of which have anti-inflammatory properties, may have a beneficial effect on human skin. The present study analyzed the effects of topical formulations containing curcumin or trans-resveratrol on the recovery and rejuvenation of skin after chemical peeling. The study was performed on rats, randomly divided into seven groups of six animals each. Superficial peeling was performed using a 50% glycolic acid gel, which was applied to the dorsal region of each animal. Rats were then treated with the experimental formulations for 15 days. On the sixteenth day, skin samples were taken and mounted on slides for histological analysis. Statistical analysis showed that the formulation containing trans-resveratrol led to increased dermal and epidermal thickness, while the formulation containing curcumin had no effects on epidermal thickness. The increased epidermal thickness may reflect greater skin vitality, although this was not directly evaluated. The increase in dermal thickness may be attributed to greater collagen production, which may increase skin firmness and elasticity, and lead to skin rejuvenation as well as wrinkle reduction. Formulations containing curcumin or trans-resveratrol may have potential for the topical treatment after peeling and of sensitive skin, in addition to being used for their antiaging properties.

关键词： Curcumin Resveratrol Peeling Histological Analysis

The Importance of the Three-Dimensional Image in the Early Diagnosis of Cementoblastoma

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Open Journal of Radiology 2012年第1期2卷 10-13页

作者： Tania mara Pimenta Amaral Claudia Silami de Magalhaes Allyson Nogueira Ricardo alves Mesquita Joao Batista de Freitas Maria Cassia Ferreira Aguiar Department of Oral Pathology and Surgery School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Brazil Department of Restorative Dentistry School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Brazil

The cementoblastoma is a very rare odontogenic neoplasm characterised by the formation of cementum-like tissue in connection with the root of a tooth. An 18-year-old boy was referred to the Clinic of Oral Pathology of the School of Dentistry, Universidade Federal de Minas Gerais, Brazil, complaining of an irradiating, itching pain from the region of the lower right first molar. He reported that the symptoms began three months prior, with a gradual increase in intensity during this period. The pain episodes were of short duration, with characteristics of toothache originating from the pulp. Upon intra-oral clinical examination, an open bite and slight increase in volume at the buccal alveolar region of the lower right first molar was observed. He also reported palpation and percussion pain symptoms in this region. Electrical, heat and cold pulp vitality tests were performed, and the tooth was vital. Periapical and panoramic radiographs showed radiolucent images with diffuse, poorly defined borders at the mesial root periapex extending to the interradicular region suggesting an inflammatory periapical lesion. In this early presentation, the three-dimensional image was fundamental to the final diagnosis of cementoblastoma.

关键词： Cementoblastoma Cone Beam CT Dental Radiography

Environmental health in public health community practice: An integrative review of the literature

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Health 2013年第9期5卷 1422-1432页

作者： Marta Regina Cezar-Vaz Clarice alves Bonow Cynthia Fontella Sant’ Anna Laurelize Pereira Rocha Marlise Capa Verde de Almeida mara Regina Santos da Silva Federal University of Pampa Uruguaiana Brazil School of Nursing Federal University of Rio Grande/RS Rio Grande City in Rio Grande do Sul Brazil

It is understood that the performance of Public Health Nursing is indispensable for the development of sustainable health communities, with regard to sub-standard living conditions in environments that include physical, chemical and biological factors influencing these conditions. This work is in an integrative review which aims to characterize how Environmental Health appears in scientific Public Health Nursing production, for the development of community health. Out of 442 articles, 30 met the search criteria. The publications covered contamination and exposure to environmental risks;strengthening of community groups for environmental health and workforce development of public health nursing for community practice in environmental health. The results summarized in the present study support the growing trend of interest in Environmental Health as knowledge which is essential to Public Health Nursing practice in the community.

关键词： Public Health Nursing Environmental Health Community Health