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Contemporary climate infuence on variability patterns of Anadenanthera colubrina ***,a key species in seasonally dry tropical forests

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Journal of Forestry Research 2022年第1期33卷 89-101页

作者： maría Victoria García maría eugenia Barrandeguy Kathleen Prinz Genética de Poblaciones y Cuantitativa-Departamento de Genética-Facultad de Ciencias Exactas Químicas y Naturales-Universidad Nacional deMisionesInstituto de Biología Subtropical(UNaM-CONICET)Nodo PosadasFélix de Azara 15523300 PosadasArgentina Consejo Nacional de Investigaciones Científicas Y Técnicas(CONICET) PosadasMisionesArgentina Institute of Systematic Botany With Herbarium Haussknecht and Botanical Gardens Friedrich-Schiller-University Jena07743 JenaGermany

The distribution of many plant species has been shaped by climate changes,and their current phenotypic and genetic variability refect microclimatically suitable *** study relates contemporary climate to variability patterns of phenotypic traits and molecular markers in the Argentinean distribution of Anadenanthera colubrina ***,as well as to identify the most relevant phenotypic trait or molecular marker associated with those *** from four populations in both biogeographic provinces,Paranaense and Yungas,were *** analyses and multiple linear regressions were carried out to determine relationships among phenotypic traits and nuclear microsatellites,respectively,to climatic variables,and to identify the phenotypic traits as well as nuclear microsatellite loci most sensitive to *** and three clusters of individuals were detected based on genetic and phenotypic data,*** clusters based on genetic data refected the biogeographic origin of *** traits were the most relevant indicators of climatic *** microsatellite locus Ac41.1 appeared to be non-neutral presenting a strong correlation with climate variable temperature *** findings show complex patterns of genetic and phenotypic variability in the Argentinean distribution of *** *** related to the present or contemporary climate,and provides an example for an integrative approach to better understand climate impact on contemporary genetic and phenotypic variability in light of global climate change.

关键词： Contemporary climate Curupay Genetic variability Phenotypic variability Seasonally dry tropical forests

Numerical Investigation of the Influence of a Magnetic Field on the Laminar Flow of a Yield-Stress Nanofluid over a Backward Facing Step

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Frontiers in Heat and Mass Transfer 2025年第1期23卷 185-206页

作者： Karim Amrani eugenia Rossi di Schio Mohamed Bouzit Abderrahim Mokhefi Abdelkader Aris Cherif Belhout Paolo Valdiserri Mechanical Manufacturing Technology Research Laboratory (LaRTFM) National Polytechnic School of Oran Maurice Audin El mnaouar Bp 1523 Es-senia Oran 31000 Algeria Department of Industrial Engineering DIN Alma Mater Studiorum-University of Bologna Viale Risorgimento 2 Bologna 40136 Italy Laboratory of maritime Sciences and Engineering LSIM Faculty of Mechanical Engineering University of Science and Technology of Oran Mohamed Boudiaf El Mnaouer Oran 31000 Algeria Mechanics Modeling and Experimentation Laboratory L2ME Faculty of Sciences and Technology Bechar University Bechar 08000 Algeria

The present study focuses on the flow of a yield-stress (Bingham) nanofluid, consisting of suspended Fe 3 O 4 nanoparticles, subjected to a magnetic field in a backward-facing step duct (BFS) configuration. The duct is equipped with a cylindrical obstacle, where the lower wall is kept at a constant temperature. The yield-stress nanofluid enters this duct at a cold temperature with fully developed velocity. The aim of the present investigation is to explore the influence of flow velocity (Re = 10 to 200), nanoparticle concentration ( φ = 0 to 0.1), magnetic field intensity (Ha = 0 to 100), and its inclination angle (γ = 0 to 90) and nanofluid yield stress (Bn = 0 to 20) on the thermal and hydrodynamic efficiency inside the backward-facing step. The numerical results have been obtained by resolving the momentum and energy balance equations using the Galerkin finite element method. The obtained results have indicated that an increase in Reynolds number and nanoparticle volume fraction enhances heat transfer. In contrast, a significant reduction is observed with an increase in Hartmann and Bingham numbers, resulting in quasi-immobilization of the fluid under the magnetic influence and radical solidification of this type of fluid, accompanied by the suppression of the vortex zone downstream of the cylindrical obstacle. This study sheds light on the complexity of this magnetically influenced fluid, with potential implications in various engineering and materials science fields.

关键词： Yield-stress nanofluid Bingham nanofluid backward-facing step BFS magnetic field heat transfer hydrodynamics

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美国推出鲜花采后贮存新型解决方案

中国花卉园艺 2025年第1期 74-76页

作者： eugenia den Ouden 妍然(译) 不详

在全球范围内,包括在美国,鲜切花的市场需求有明显的季节性波动,情人节是玫瑰销售的高峰期,复活节、母亲节和圣诞节.也是鲜花需求旺季。如何满足节日销售旺季的需求,及时提供新鲜的高品质鲜花?这对花卉行业来说是一项挑战。由此,许多延... 详细信息

在全球范围内,包括在美国,鲜切花的市场需求有明显的季节性波动,情人节是玫瑰销售的高峰期,复活节、母亲节和圣诞节.也是鲜花需求旺季。如何满足节日销售旺季的需求,及时提供新鲜的高品质鲜花?这对花卉行业来说是一项挑战。由此,许多延长切花保鲜期的技术应运而生。

关键词：花卉行业切花保鲜鲜切花全球范围鲜花母亲节情人节季节性波动

Epidermal stem cells and skin tissue engineering in hair follicle regeneration

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World Journal of Stem Cells 2015年第4期7卷 711-727页

作者： maría eugenia Balaná Hernán Eduardo Charreau Gustavo Jose Leirós Fundación Pablo Cassará-Instituto de Ciencia y Tecnología Dr.César Milstein Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)Saladillo 2468 C1440FFXCiudad de Buenos AiresArgentina Technology Intelligence Unit ClarkeModet&C°Lavalle 1903°PisoC1047AAD Buenos AiresArgentina

The reconstitution of a fully organized and functional hair follicle from dissociated cells propagated under defined tissue culture conditions is a challenge stillpending in tissue engineering. The loss of hair follicles caused by injuries or pathologies such as alopecia not only affects the patients' psychological well-being, but also endangers certain inherent functions of the skin. It is then of great interest to find different strategies aiming to regenerate or neogenerate the hair follicle under conditions proper of an adult individual. Based upon current knowledge on the epithelial and dermal cells and their interactions during the embryonic hair generation and adult hair cycling, many researchers have tried to obtain mature hair follicles using different strategies and approaches depending on the causes of hair loss. This review summarizes current advances in the different experimental strategies to regenerate or neogenerate hair follicles, with emphasis on those involving neogenesis of hair follicles in adult individuals using isolated cells and tissue engineering. Most of these experiments were performed using rodent cells, particularly from embryonic or newborn origin. However, no successful strategy to generate human hair follicles from adult cells has yet been reported. This review identifies several issues that should be considered to achieve this objective. Perhaps the most important challenge is to provide threedimensional culture conditions mimicking the structure of living tissue. Improving culture conditions that allow the expansion of specific cells while protecting their inductive properties, as well as methods for selecting populations of epithelial stem cells, should give us the necessary tools to overcome the difficulties that constrain human hair follicle neogenesis. An analysis of patent trends shows that the number of patent applications aimed at hair follicle regeneration and neogenesis has been increasing during the last decade. This fiel

关键词： Adult stem cells Skin grafts Epidermis Multipotential differentiation Tissue regeneration Dermal papilla Epithelial-mesenchymal interactions

Experimental bio-artificial liver:Importance of the architectural design on ammonia detoxification performance

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World Journal of Hepatology 2018年第10期10卷 719-730页

作者： maría Dolores Pizarro maría eugenia Mamprin Lucas Damián Daurelio Joaquín Valentín Rodriguez maría Gabriela Mediavilla Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET) Rosario S2002 LRKArgentina Laboratorio de Investigaciones en Fisiología y Biología Molecular Vegetal(LIFiBVe) Facultad de Ciencias AgrariasUniversidad Nacional del LitoralEsperanza 3080Argentina Facultad de Ciencias Bioquímicas y Farmacéuticas Universidad Nacional de RosarioRosario S2002 LRKArgentina Centro Binacional de Criobiología Clínica y Aplicada(CAIC) Universidad Nacional de RosarioRosario S2011 BXNArgentina Instituto de Biología Molecular y Celular de Rosario(IBR CONICET-UNR)Consejo Nacional de Investigaciones Científicas y Tecnológicasy Universidad Nacional de RosarioRosario S2002 LRKArgentina

AIM To determine the influence of the construction design over the biological component's performance in an experimental bio-artificial liver(BAL) *** Two BAL models for liver microorgans(LMOs) were constructed. First, we constructed a cylindrical BAL and tested it without the biological component to establish its correct functioning. Samples of blood and biological compartment(BC) fluid were taken after 0, 60, and 120 min of perfusion. Osmolality, hematocrit, ammonia and glucose concentrations, lactate dehydrogenase(LDH) release(as a LMO viability parameter), and oxygen consumption and ammonia metabolizing capacity(as LMO functionality parameters) were determined. CPSI and OTC gene expression and function were measured. The second BAL, a "flat bottom" model, was constructed using a 25 cm2 culture flask while maintaining all other components between the models. The BC of both BALs had the same capacity(approximately 50 cm3) and both were manipulated with the same perfusion system. The performances of the two BALs were compared to show the influence of *** The cylindrical BAL showed a good exchange of fluids and metabolites between blood and the BC, reflected by the matching of osmolalities, and glucose and ammonia concentration ratios after 120 min of perfusion. No hemoconcentration was detected, the hematocrit levels remained stable during the whole study, and the minimal percentage of hemolysis(0.65% ± 0.10%) observed was due to the action of the peristaltic pump. When LMOs were used as biological component of this BAL they showed similar values to the ones obtained in a Normothermic Reoxygenation System(NRS) for almost all the parameters assayed. After 120 min, the results obtained were: LDH release(%): 14.7 ± 3.1 in the BAL and 15.5 ± 3.2 in the NRS(n = 6); oxygen consumption(μmol/min?g wet tissue): 1.16 ± 0.21 in the BAL and 0.84 ± 0.15 in the NRS(n = 6); relative expression of Cps1 and Otc: 0.63 ± 0.12 and 0.67 ± 0.20, respectively,

关键词： Bio-artificial liver Ammonia detoxification Device design Ornithine Transcarbamylase Rat liver microorgans Carbamyl Phosphate SynthetaseⅠ

Performance of cold-preserved rat liver Microorgans as the biological component of a simplified prototype model of bioartificial liver

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World Journal of Hepatology 2016年第33期8卷 1442-1451页

作者： maría Dolores Pizarro maría Gabriela Mediavilla Alejandra Beatriz Quintana ángel Luis Scandizzi Joaquín Valentín Rodriguez maría eugenia Mamprin Centro Binacional de Criobiología Clínica y Aplicada Instituto de Biología Molecular y Celular de Rosario Consejo Nacional de Investigaciones Científicas y Técnicas Morfología Facultad de Ciencias Bioquímicas y Farmacéuticas área Farma-cología Facultad de Ciencias Bioquímicas y FarmacéuticasUniversidad Nacional de Rosario

AIMTo develop a simplified bioartificial liver (BAL) device prototype, suitable to use freshly and preserved liver Microorgans (LMOs) as biological component. METHODSThe system consists of 140 capillary fibers through which goat blood is pumped. The evolution of hematocrit, plasma and extra-fiber fluid osmolality was evaluated without any biological component, to characterize the prototype. LMOs were cut and cold stored 48 h in BG35 and ViaSpan® solutions. Fresh LMOs were used as controls. After preservation, LMOs were loaded into the BAL and an ammonia overload was added. To assess LMOs viability and functionality, samples were taken to determine lactate dehydrogenase (LDH) release and ammonia detoxification capacity. RESULTSThe concentrations of ammonia and glucose, and the fluids osmolalities were matched after the first hour of perfusion, showing a proper exchange between blood and the biological compartment in the minibioreactor. After 120 min of perfusion, LMOs cold preserved in BG35 and ViaSpan® were able to detoxify 52.9% ± 6.5% and 53.6% ± 6.0%, respectively, of the initial ammonia overload. No significant differences were found with Controls (49.3% ± 8.8%, P ® cold preserved LMOs, respectively (n = 6, P CONCLUSIONThis prototype relied on a simple design and excellent performance. It’s a practical tool to evaluate the detoxification ability of LMOs subjected to different preservation protocols.

关键词： Rat liver Microorgans Cold preservation BG35 preservation solution Bioartificial liver device Acute liver failure

A shape shifting organelle: unusual mitochondrial phenotype determined with three-dimensional electron microscopy reconstruction

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Neural Regeneration Research 2016年第6期11卷 900-901页

作者： eugenia Trushina Department of Neurology Mayo Clinic Department of Molecular Pharmacology and Experimental Therapeutics Mayo Clinic

Mitochondria,the powerhouses of the cell,are dynamic organelles that constantly move and change their size and morphology to meet energetic demands of the cell（Wai and Langer,2016）.Recent studies demonstrated that energy production is dependent on the ability of mitochondria to undergo cycles of fission and fusion collectively termed“mitochondrial dynamics”（Youle and van der Bliek,2012）.

关键词： mitochondrial phenotype Langer constantly fission hippocampal neuronal unusual protective deprivation

Liver histological changes and lipid peroxidation in the amphibian Ambystoma mexicanum induced by sediment elutriates from the Lake Xochimilco

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Journal of Environmental Sciences 2016年第8期28卷 156-164页

作者： Esperanza Ortiz-Ordonez eugenia López-López Jacinto Elías Sedeno-Díaz Esther Uría Ignacio Andrés Morales maría Estela Pérez Mineko Shibayama Instituto Politécnico Nacional Depto.de Formación Básica e InterdisciplinariaCICS-USTAv.de los Maestros s/n.Col.Sto Instituto Politécnico Nacional Laboratorio de Evaluación de la Salud de Ecosistemas AcuáticosENCBProl.de Carpio y Plan de Ayala s/n.Col.Sto Instituto Politécnico Nacional Coordinación Politécnica para la SustentabilidadEdificio de la Biblioteca Nacional3er PisoAv.Instituto Politécnico Nacional s/nesq.Wilfrido MassieuCol Instituto Politécnico Nacional Laboratorio de HistologíaENCBProl.de Carpio y Plan de Ayala s/n.Col.Sto Unidad de Servicio Académico Acuario BiologíaFacultad de CienciasUniversidad Autónoma de MéxicoCiudad Universitaria Laboratorio de Infectómica Centro de Investigación y de Estudios Avanzados del IPNAv.Instituto Politécnico Nacional

Lacustrine sediments accumulate pollutants that input from the lake watershed and can be released to the water column by sediment resuspension; thus, pollutants can change their bioavailability and exert adverse effects to aquatic biota. Shallow-urban lakes are particularly susceptible to receive pollutants from urban discharges and sediment resuspension. Lake Xochimilco, in Mexico City, an urban-shallow lake, faces multiple problems： urban sprawl, overexploitation of aquifers, drying of springs, discharge of wastewater from treatment plants, and sediment resuspension. The aquatic biota living in this ecosystem is continuously exposed to the release of pollutants from the sediments. We assessed the risk that pollutants released from sediments from Lake Xochimilco, Touristic（TZ） and Agriculture zone（AZ）, can exert on a native amphibian species of the lake（Ambystoma mexicanum） through exposure bioassays to sediment elutriates. We evaluate alterations in the amphibian by three approaches： biochemical（level of lipid peroxidation,LPO）, cellular（ultrastructure） and the liver histology of A. mexicanum and we compare them with a batch control. Additionally, we assessed heavy metals（Pb, Cd and Hg） in *** from TZ showed the highest concentrations of the metals assessed. Organisms exposed to sediment elutriates from either study sites showed higher LPO values than control organisms（p 〈 0.05）. Organisms exposed to elutriates from the TZ showed the most conspicuous damages： hepatic vasodilation of sinusoids, capillaries with erythrocytes,leukocyte infiltration and cytoplasmic vacuolation in hepatocytes. The biological responses assessed reflected the risk that faces A. mexicanum when is exposed for prolonged periods to sediment resuspension in Lake Xochimilco.

关键词： Elutriates Amphibian Liver Histological alterations Ultrastructure Oxidative stress Metals Urban lake

“Second-generation” stem cells for cardiac repair

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World Journal of Stem Cells 2015年第2期7卷 352-367页

作者： Alberto Núez García Ricardo Sanz-Ruiz maría eugenia Fernández Santos Francisco Fernández-Avilés Instituto parala Investigación Biomédica del Hospital General Universitario Gregorio mara?ón Department of Internal Medicine Complutense University-School of Medicine Department of Cardiology Hospital General Universitario Gregorio Mara?ón Laboratory of Bioartificial Organs and Scaffolds Hospital General Universitario Gregorio Mara?ón

Over the last years, stem cell therapy has emerged asan inspiring alternative to restore cardiac function after myocardial infarction. A large body of evidence has been obtained in this field but there is no conclusive data on the efficacy of these treatments. Preclinical studies and early reports in humans have been encouraging and have fostered a rapid clinical translation, but positive results have not been uniformly observed and when present, they have been modest. Several types of stem cells, manufacturing methods and delivery routes have been tested in different clinical settings but direct comparison between them is challenging and hinders further research. Despite enormous achievements, major barriers have been found and many fundamental issues remain to be resolved. A better knowledge of the molecular mechanisms implicated in cardiac development and myocardial regeneration is critically needed to overcome some of these hurdles. Genetic and pharmacological priming together with the discovery of new sources of cells have led to a "second generation" of cell products that holds an encouraging promise in cardiovascular regenerative medicine. In this report, we review recent advances in this field focusing on the new types of stem cells that are currently being tested in human beings and on the novel strategies employed to boost cell performance in order to improve cardiac function and outcomes after myocardial infarction.

关键词： Stem cells Cardiac repair Myocardialinfarction Heart failure Second generation