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Maize seed coatings and seedling sprayings with chitosan and hydrogen peroxide:their influence on some phenological and biochemical behaviors

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Journal of Zhejiang University-Science b(biomedicine & biotechnology) 2013年第2期14卷 87-96页

作者： Eva-Guadalupe LIZRRAGA-PAULíN Susana-Patricia MIRANDA-CASTRO Ernesto MORENO-marTíNEZ Alma-Virginia lara-SAGAHóN Irineo TORRES-PACHECO Laboratorio de biotecnología Facultad de Estudios Superiores CuautitlánCampo 1Universidad Nacional Autónoma de MéxicoEstado de México 54740México Unidad de Investigación en Granos y Semillas Facultad de Estudios Superiores CuautitlánCentro de Asimilación TecnológicaUniversidad Nacional Autónoma de MéxicoEstado de México 54740México CA Ingeniería de biosistemas División de Investigación y PosgradoFacultad de IngenieríaUniversidad Autónoma de QuerétaroQuerétaro 76010México

Objective： To evaluate the effect of chitosan （CH） and hydrogen peroxide （H2O） seed coatings and seedling sprinklings on two different maize varieties by measuring their phenology, the H202 presence, the catalase （CAT） activity, and the protein quantity. Methods： Seven groups of ten seeds for each maize variety were treated with CH （2% （20 g/L） and 0.2% （2 g/L）） or H2O2 （8 mmol/L） by coating, sprinkling, or both. Germination and seedling growth were measured. One month after germination, the presence of H202 in seedlings in the coated seed treatments was evaluated. Protein content and CAT activity were determined under all treatments. Results： H2O2 seed coating en- hanced the germination rate and increased seedling and stem length in the quality protein maize （QPM） variety. Seedlings had a higher emergence velocity under this treatment in both varieties. CH and H2O2 sprinklings did not have an effect on seedling phenology. Exogenous application of H2O2 promoted an increase of endogenousH2O2. OH and H2O2seedling sprinkling increased the protein content in both maize varieties, while there was no significant effect on the CAT activity of treated seeds and seedlings. Conclusions： CH and H2O2 enhance some phenological and biochemical features of maize depending on their method of application.

关键词： Zea mays Chitosan Hydrogen peroxide Phenology Catalase Protein

Use of recombinant microRNAs as antimetabolites to inhibit human non-small cell lung cancer

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Acta Pharmaceutica Sinica b 2023年第10期13卷 4273-4290页

作者： Yixin Chen Mei-Juan Tu Fangwei Han Zhenzhen Liu Neelu batra Primo N.lara Hong-Wu Chen Huichang bi Ai-Ming Yu Department of biochemistry and Molecular Medicine School of MedicineUC DavisSacramentoCA 95817USA School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou 510006China School of Public Health UNT Health Science CenterFort WorthTX 76107USA Department of Internal Medicine School of MedicineUC DavisSacramentoCA 95817USA

During the development of therapeutic microRNAs(miRNAs or miRs),it is essential to define their pharmacological ***,miRNA research and therapy mainly use miRNA mimics synthesized in *** experimental screening of unique recombinant miRNAs produced in vivo,three lead antiproliferative miRNAs against human NSCLC cells,miR-22-3p,miR-9-5p,and miR-218-5p,were revealed to target folate metabolism by bioinformatic *** miR-22-3p,miR-9-5p,and miR-218-5p were shown to regulate key folate metabolic enzymes to inhibit folate metabolism and subsequently alter amino acid metabolome in NSCLC A549 and H1975 *** tracing studies further confirmed the disruption of one-carbon transfer from serine to folate metabolites by all three miRNAs,inhibition of glucose uptake by miR-22-3p,and reduction of serine biosynthesis from glucose by miR-9-5p and-218-5p in NSCLC *** greater activities to interrupt NSCLC cell respiration,glycolysis,and colony formation than miR-9-5p and-218-5p,recombinant miR-22-3p was effective to reduce tumor growth in two NSCLC patient-derived xenograft mouse models without causing any *** results establish a common antifolate mechanism and differential actions on glucose uptake and metabolism for three lead anticancer miRNAs as well as antitumor efficacy for miR-22-3p nanomedicine,which shall provide insight into developing antimetabolite RNA therapies.

关键词： RNA therapy Folate metabolism Amino acid Glycolysis miR-22 miR-9 miR-218 Lung cancer

N-acetyl-L-cysteine combined with mesalamine in the treatment of ulcerative colitis: Randomized,placebo-controlled pilot study

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World Journal of Gastroenterology 2008年第18期14卷 2851-2857页

作者： Luis G Guijarro Jose Mate Javier P Gisbert Jose Luis Perez-Calle Ignacio marín-Jimenez Encarna Arriaza Tomás Olleros mario Delgado maria S Castillejo David Prieto-Merino Venancio Gonzalez lara Amado Salvador Pea Department of biochemistry and Molecular biology CIBERehd Alcalá University Alcalá de Henares Madrid Spain Gastroenterology Unit CIBERehd La Princesa University Hospital Autonomous University Madrid Spain Gastroenterology Unit Gregorio Maraón University Hospital Complutense University Madrid Spain Group Farmasierra SL. Ctra N-Ⅱ Km 26.200 San Sebastián de los Reyes Madrid Spain Laboratory of Immunogenetics Department of Pathology VU University Medical Center Amsterdam The Netherlands

AIM: To evaluate the effectiveness and safety of oral N-acetyl-L-cysteine (NAC) co-administration with mesalamine in ulcerative colitis (UC) patients. METHODS: Thirty seven patients with mild to moderate UC were randomized to receive a four-wk course of oral mesalamine (2.4 g/d) plus N-acetyl-L-cysteine (0.8 g/d) (group A) or mesalamine plus placebo (group b). Patients were monitored using the Modified Truelove-Witts Severity Index (MTWSI). The primary endpoint was clinical remission (MTWSI ≤ 2) at 4 wk. Secondary endpoints were clinical response (defined as a reduction from baseline in the MTWSI of ≥ 2 points) and drug safety. The serum TNF-α, interleukin-6, interleukin-8 and MCP-1 were evaluated at baseline and at 4 wk of treatment. RESULTS: Analysis per-protocol criteria showed clinical remission rates of 63% and 50% after 4 wk treatment with mesalamine plus N-acetyl-L-cysteine (group A) and mesalamine plus placebo (group b) respectively (OR = 1.71; 95% CI: 0.46 to 6.36; P = 0.19; NNT = 7.7). Analysis of variance (ANOVA) of data indicated a significant reduction of MTWSI in group A (P = 0.046) with respect to basal condition without significant changes in the group b (P = 0.735) during treatment. Clinical responses were 66% (group A) vs 44% (group b) after 4 wk of treatment (OR = 2.5; 95% CI: 0.64 to 9.65; P = 0.11; NNT = 4.5). Clinical improvement in group A correlated with a decrease of IL-8 and MCP-1. Rates of adverse events did not differ significantly between both groups. CONCLUSION: In group A (oral NAC combined with mesalamine) contrarily to group b (mesalamine alone), the clinical improvement correlates with a decrease of chemokines such as MCP-1 and IL-8. NAC addition not produced any side effects.

关键词： Ulcerative colitis Interleukin Mesalamine N-acetyl-L-cysteine

Celecoxib-induced gastrointestinal, liver and brain lesions in rats, counteraction by bPC 157 or L-arginine, aggravation by L-NAME

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World Journal of Gastroenterology 2017年第29期23卷 5304-5312页

作者： Domagoj Drmic Danijela Kolenc Spomenko Ilic lara bauk marko Sever Anita Zenko Sever Kresimir Luetic Jelena Suran Sven Seiwerth Predrag Sikiric Departments of Pharmacology and Pathology Medical Faculty University of Zagreb Faculty of Medicine J.J. Strossmayer University of Osijek

To counteract/reveal celecoxib-induced toxicity and NO system involvement. METHODSCelecoxib (1 g/kg b.w. ip) was combined with therapy with stable gastric pentadecapeptide bPC 157 (known to inhibit these lesions, 10 μg/kg, 10 ng/kg, or 1 ng/kg ip) and L-arginine (100 mg/kg ip), as well as NOS blockade [N(G)-nitro-L-arginine methyl ester (L-NAME)] (5 mg/kg ip) given alone and/or combined immediately after celecoxib. Gastrointestinal, liver, and brain lesions and liver enzyme serum values in rats were assessed at 24 h and 48 h thereafter. RESULTSThis high-dose celecoxib administration, as a result of NO system dysfunction, led to gastric, liver, and brain lesions and increased liver enzyme serum values. The L-NAME-induced aggravation of the lesions was notable for gastric lesions, while in liver and brain lesions the beneficial effect of L-arginine was blunted. L-arginine counteracted gastric, liver and brain lesions. These findings support the NO system mechanism(s), both NO system agonization (L-arginine) and NO system antagonization (L-NAME), that on the whole are behind all of these COX phenomena. An even more complete antagonization was identified with bPC 157 (at both 24 h and 48 h). A beneficial effect was evident on all the increasingly negative effects of celecoxib and L-NAME application and in all the bPC 157 groups (L-arginine + bPC 157; L-NAME + bPC 157; L-NAME + L-arginine + bPC 157). Thus, these findings demonstrated that bPC 157 may equally counteract both COX-2 inhibition (counteracting the noxious effects of celecoxib on all lesions) and additional NOS blockade (equally counteracting the noxious effects of celecoxib + L-NAME). CONCLUSIONbPC 157 and L-arginine alleviate gastrointestinal, liver and brain lesions, redressing NSAIDs’ post-surgery application and NO system involvement.

关键词： bPC 157 Celecoxib L-arginine N(G)-nitro-L-arginine methyl ester Rats

Radiative Recombination Mechanisms of Large InAs/GaAs Quantum Dots

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World Journal of Condensed Matter Physics 2011年第4期1卷 161-166页

作者： Sandro martini Angelo E. b. marques marcelo marques Alain A. Quivy lara Kühl Teles 不详

The optical properties of large InAs/GaAs quantum dots were investigated by low-temperature photoluminescence as a function of the excitation-power density. The presence of excited states was clearly detected below the saturation regime of the ground state. We analyzed the dependence of the integrated-photoluminescence intensity on the excitation-power density and the type of radiative recombination involving the electronic ground state and the excited states inside the quantum dots. We concluded that the probability to have more than one exciton by dots must be considered, and the usual equation , must be revised to correctly describe the origin of the recombination and must include other factors as scattering, relaxation time, radiative recombination rate, and others.

关键词： Quantum Dots Photoluminescence Carrier Dynamics

Highly sensitive ratiometric fluorescent fiber matrices for oxygen sensing with micrometer spatial resolution

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bio-Design and Manufacturing 2024年第3期7卷 292-306页

作者： Giuliana Grasso Valentina Onesto Stefania Forciniti Eliana D’Amone Francesco Colella lara Pierantoni Valeria Famà Giuseppe Gigli Rui L.Reis J.Miguel Oliveira Loretta L.del Mercato nstitute of Nanotechnology National Research Council(CNR-NANOTEC)c/o Campus Ecoteknevia Monteroni73100 LecceItaly Department of Mathematics and Physics“Ennio De Giorgi” University of Salentoc/o Campus Ecoteknevia Monteroni73100 LecceItaly 3b’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3b’s-PT Government Associate Laboratory 4806-909 Braga/GuimarãesPortugal Department of Experimental Medicine University of Salentoc/o Campus Ecoteknevia Monteroni73100 LecceItaly

Oxygen(O_(2))-sensing matrices are promising tools for the live monitoring of extracellular O_(2) consumption levels in long-term cell *** this study,ratiometric O_(2)-sensing membranes were prepared by electrospinning,an easy,low-cost,scalable,and robust method for fabricating ***(ε-caprolactone)and poly(dimethyl)siloxane polymers were blended with tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II)dichloride,which was used as the O_(2)-sensing probe,and rhodamine b isothiocyanate,which was used as the reference *** functionalized scaffolds were morphologically characterized by scanning electron microscopy,and their physicochemical profiles were obtained by Fourier transform infrared spectroscopy,thermogravimetric analysis,and water contact angle *** sensing capabilities were investigated by confocal laser scanning microscopy,performing photobleaching,reversibility,and calibration curve studies toward different dissolved O_(2)(DO)*** sensing nanofibers showed a high response to changes in DO concentrations in the physiological-pathological range from 0.5%to 20%and good stability under ratiometric *** addition,the sensing systems were highly biocompatible for cell growth promoting adhesiveness and growth of three cancer cell lines,namely metastatic melanoma cell line SK-MEL2,breast cancer cell line MCF-7,and pancreatic ductal adenocarcinoma cell line Panc-1,thus recreating a suitable biological environment in *** O_(2)-sensing biomaterials can potentially measure alterations in cell metabolism caused by changes in ambient O_(2)content during drug testing/validation and tissue regeneration processes.

关键词： Electrospinning Ruthenium(II)dichloride Oxygen sensors Ratiometric imaging Fluorescence

Landscape Analysis for PaV1 Infection in Lobsters Panulirus argus from the Artisanal Fishery of the Eastern Coast of Yucatan, Mexico

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Open Journal of marine Science 2016年第3期6卷 386-394页

作者： Ruth A. Pérez-Campos Oswaldo Huchim-lara Silvia Salas maría Liceaga-Correa Héctor Hernández-Nuñez Cristina Pascual-Jiménez Pascual-Jiménez Rossanna Rodríguez-Canul Laboratorio de Inmunología y biología Molecular Mérida Yucatán México Laboratorio de Pesquerías Mérida Yucatán México Laboratorio de Percepción Remota y Sistemas de Información Geográfica Centro de Investigación y de Estudios Avanzados del IPN-Mérida (CINVESTAV-IPN) Merida Mexico Universidad Nacional Autónoma de México Facultad de Ciencias Unidad Académica Sisal Sisal Mexico

Panulirus argus virus 1 (PaV1) is considered a major threat to spiny lobsters Panulirus argus. In this study Geospatial analysis was used to analyze PaV1 distribution in an artisanal fishery of spiny lobster Panulirus argus population from the north coast of the Yucatan Peninsula. Adult and sub-adult P. argus and seabed coverage data were collected from thirty artisanal fishing sites. Five seabed coverage types were identified: seagrass;sand/seagrass mixture;sand only;coral/sand mixture;and seaweed. No juveniles were examined. Of the 358 collected lobsters, PaV1 was identified in four organisms (three sub-adults and one adult) from two fishing sites (termed A & b), both found in a seagrass coverage area. Overall prevalence was of 1.12%. Prevalence was of 20% (2/10) at one site and of 12.6% (2/16) at the other.

关键词： Panulirus argus PaV1 Geospatial Analysis Artisanal Fishery Seagrass

Prolonged coexistent central diabetes insipidus and cerebral salt wasting syndrome following neurosurgery

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Open Journal of Pediatrics 2013年第2期3卷 74-77页

作者： D. López de lara b. Joyanes A. Llaneza O. Pérez b. Llorente I. Runkle Department of Endocrinology Hospital Clínico San Carlos Madrid Spain Department of Pediatrics Hospital Clínico San Carlos Madrid Spain

The coexistence of different water homeostasis abnormalities following neurosurgery represents a diagnostic and therapeutic challenge for intensive care units. This paper reports the case of a 13 year-old boy who underwent surgery for a suprasellar tumour and, immediately after surgery, developed a cerebral abscess, persistent diabetes insipidus (DI) as well as cerebral salt wasting syndrome (CSWS). The early onset of CSWS following DI has been associated with a poor prognosis and increased mortality. In cases in which these abnormalities coexist, the increased polyuria secondary to the rise in natriuresis associated with CSWS might be erroneously interpreted as a sign of poor control of the DI, thereby leading to therapeutic mistakes. Treatment basically consists of restoring electrolytes and the joint administration of desmopressin and fludrocortisone.

关键词： Electrolyte Disorder Diabetes Insipidus Cerebral Salt Wasting Syndrome Neurosurgery

SENP7 overexpression protects cancer cells from oxygen and glucose deprivation and associates with poor prognosis in colon cancer

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Genes & Diseases 2022年第6期9卷 1419-1422页

作者： Francisco Gallardo-Chamizo Nieves lara-Urena Juan F.Correa-Vazquez Jose C.Reyes benoit R.Gauthier mario Garcia-Dominguez Cell Dynamics and Signaling Department CABIMERCSICUniversidad de Sevilla-Universidad Pablo de Olavide-Junta de AndaluciaSeville 41092Spain Genome biology Department CABIMERCSIC-Universidad de Sevilla-Universidad Pablo de Olavide-Junta de AndaluciaSeville 41092Spain Cell Therapy and Regeneration Department CABIMERCSIC-Universidad de Sevilla-Universidad Pablo de Olavide-Junta de AndaluciaSeville 41092Spain Centro de Investigacion biomedica en Red de Diabetes y Enfermedades Metabo´licas Asociadas(CIbERDEM) Madrid 28029Spain

To the Editor,Constricted oxygen and glucose supplies are common conditions that healthy cells following a stress such an ischemic insult as well as cancer cells within a solid tumor need to overcome in order to *** conditions are a serious challenge for cells,which accurately measure the impact to make a life or death *** cancer cells,aberrant gene expression and/or protein regulation,frequently linked to appearing mutations,may confer selective advantages to survive under these adverse *** the past years,cumulative evidence indicates that protein modification by covalent attachment of the Small Ubiquitin-like MOdifier(SUMO)plays a role in cell survival after oxygen and glucose deprivation(OGD).

关键词： cancer deprivation colon