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Differential response of injured and healthy retinas to syngeneic and allogeneic transplantation of a clonal cell line of immortalized olfactory ensheathing glia:a double-edged sword

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Neural Regeneration Research 2025年第8期20卷 2395-2407页

作者： maría Norte-Muñoz maría Portela-Lomba Paloma Sobrado-Calvo Diana Simón Johnny Di Pierdomenico Alejandro Gallego-Ortega mar Pérez JoséMCabrera-Maqueda Javier Sierra Manuel Vidal-Sanz maría teresa.moreno-flores marta Agudo-Barriuso Grupo de Investigación Oftalmología Experimental Departamento de OftalmologíaOptometríaOtorrinolaringología y Anatomía PatológicaFacultad de MedicinaUniversidad de MurciaInstituto Murciano de Investigación Biosanitaria(IMIB)Campus de Ciencias de la SaludMurciaSpain Experimental Sciences Faculty Universidad Francisco de VitoriaPozuelo de AlarcónMadridSpain Anatomy Histology and Neuroscience DepartmentMedicine FacultyUniversidad Autónoma de MadridMadridSpain Center of Neuroimmunology Service of NeurologyLaboratory of Advanced Imaging in Neuroimmunological DiseasesHospital Clínic of BarcelonaInstitut d’Investigacions Biomèdiques August Pi i Sunyer(IDIBAPS)and Universitat de BarcelonaBarcelonaSpain Medicine Faculty Universidad Francisco de VitoriaPozuelo de AlarcónMadridSpain

Olfactory ensheathing glia promote axonal regeneration in the mammalian central nervous system,including retinal ganglion cell axonal growth through the injured optic ***,it is unknown whether olfactory ensheathing glia also have neuroprotective *** ensheathing glia express brain-derived neurotrophic factor,one of the best neuroprotectants for axotomized retinal ganglion ***,we aimed to investigate the neuroprotective capacity of olfactory ensheating glia after optic nerve *** ensheathing glia cells from an established rat immortalized clonal cell line,TEG3,were intravitreally injected in intact and axotomized retinas in syngeneic and allogeneic mode with or without microglial inhibition or immunosuppressive *** and gene expression analyses were *** bulb-derived primary olfactory ensheathing glia and TEG3 express major histocompatibility complex classⅡ*** and syngenically transplanted TEG3 cells survived in the vitreous for up to 21 days,forming an *** axotomized retinas,only the allogeneic TEG3 transplant rescued retinal ganglion cells at 7 days but not at 21 *** these retinas,microglial anatomical activation was higher than after optic nerve crush *** intact retinas,both transplants activated microglial cells and caused retinal ganglion cell death at 21 days,a loss that was higher after allotransplantation,triggered by pyroptosis and partially rescued by microglial inhibition or ***,neuroprotection of axotomized retinal ganglion cells did not improve with these *** different neuroprotective properties,different toxic effects,and different responses to microglial inhibitory treatments of olfactory ensheathing glia in the retina depending on the type of transplant highlight the importance of thorough preclinical studies to explore these variables.

关键词： cell therapy immune recognition major histocompatibility complex class II(MHCII) neuroprotection olfactory ensheathing glia retinal ganglion cells

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Ubiquitin-proteasome system and oxidative stress in liver transplantation

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World Journal of Gastroenterology 2018年第31期24卷 3521-3530页

作者： Norma Alva Arnau Panisello-Roselló marta flores Joan Roselló-Catafau teresa Carbonell Department of Cell Biology Physiology and ImmunologyUniversity of Barcelona Experimental Pathology Department Institute of Biomedical Research of Barcelona

A major issue in organ transplantation is the development of a protocol that can preserve organs under optimal conditions. Damage to organs is commonly a consequence of flow deprivation and oxygen starvation following the restoration of blood flow and reoxygenation. This is known as ischemia-reperfusion injury(IRI): a complex multifactorial process that causes cell damage. While the oxygen deprivation due to ischemia depletes cell energy, subsequent tissue oxygenation due to reperfusion induces many cascades, from reactive oxygen species production to apoptosis initiation. Autophagy has also been identified in the pathogenesis of IRI, although such alterations and their subsequent functional significance are controversial. Moreover, proteasome activation may be a relevant pathophysiological mechanism. Different strategies have been adopted to limit IRI damage, including the supplementation of commercial preservation media with pharmacological agents or additives. In this review, we focus on novel strategies related to the ubiquitin proteasome system and oxidative stress inhibition, which have been used to minimize damage in liver transplantation.

关键词： Liver transplant Ischemia-reperfusion injury Oxidative stress Proteasome Redox regulation Ubiquitin

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Laser-Induced Breakdown Spectroscopy (LIBS) Applied in the Differentiation of Arabicaand RobustaCoffee

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Optics and Photonics Journal 2017年第10期7卷 181-195页

作者： Abigail maría Díaz Guerrero Luis Vidal Ponce Cabrera teresa flores Reyes Rogelio Ortega Izaguirre Research Center forApplied Science and Advanced Technology Altamira Unit National Polytechnic Institute Altamira Mexico

Coffee is one of the most consumed and commercialized crops in the world, which is why there is a potential risk to find arabica coffee (an expensive variety) adulterated with robusta coffee (a cheaper variety). The currently used technique for certifying coffee, High Performance Liquid Chromatography (HPLC), requires the sample to be subjected to a chemical treatment prior to analysis;in addition, the equipment is bulky and can not be moved easily. Laser Induced Breakdown Spectroscopy (LIBS) is a technique which does not require that samples be subjected to a chemical pretreatment, and equipment is small and portable, this can save valuable time in coffee trading. The purpose of this research was to demonstrate that LIBS can be applied to solve various problems related with the coffee authentication. Green coffee pills with different concentrations of arabica and robusta varieties were analyzed by LIBS, the results were used in the construction of calibration curves for the detection of the degree of simulated adulteration in coffee. It was found that the relative intensities of Ca (392.4 nm), Sr (407.1 nm), N (500.5 nm) and Na (588.7 nm), as well as the intensity ratios of Ca II (392.4 nm)/N I (500.5 nm), Sr I (407.1 nm)/N I (500.5 nm)and N I (500.5 nm)/Na I (588.7 nm) can be used for this purpose. It is concluded that the differentiation of coffee and the detection of its adulteration is possible with the use of LIBS. Further, with the use of an Artificial Neural Network (ANN) of type Multilayer Perceptron, it was possible to correctly classify the spectra of arabica and robusta roasted coffee.

关键词： Laser Induced Breakdown Spectroscopy (LIBS) Calibration Curves Artificial Neural Network (ANN) Multilayer Perceptron

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Evaluation of the Hydroxynitrile Lyase Activity in Cell Cultures of Capulin (Prunus serotina)

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生物工程学报 2008年第7期24卷 1199-1201页

作者： Liliana Hemandez Hector Luna Arturo Navarro-Ocafia Ma teresa de Jesus Olivera-flores Ivon Ayala UniversidadAutdnoma Metropolitana UnidadXochimilco Depto. Sistemas Biologicos A. P. 23/181 Mgxico D. F. Mexico Facultad de Qufmica Universidad Nacional Autdnoma de Mexico Ciudad Universitaria Mgxico D. F. 04510 Mexico

Enzymatic preparations obtained from young plants and cell cultures of capulin were screened for hydroxynitrile lyase activity. The threeweek old plants, grown under sterile conditions, were used to establish a solid cell culture. Crude preparations obtained from this plant material were evaluated for the transformation of benzaldehyde to the corresponding cyanohydrin (mandelonitrile). The results show that the crude material from roots, stalks, and leaves of young plants and calli of roots, stalks, internodes and petioles biocatalyzed the addition of hydrogen cyanide (HCN) to benzaldehyde with a modest to excellent enantioselectivity.

关键词：细胞裂解酶活性柳叶野黑桃

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XEN^(■) implant and trabeculectomy medium-term quality of life assessment and comparison of results

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International Journal of Ophthalmology(English edition) 2018年第12期11卷 1941-1944页

作者： Ana Luisa Basilio Nuno Moura-Coelho Ines Passos mariana Sa Cardoso Isabel Domingues maria Reina Rita flores teresa Gomes Ophthalmology Department Central Lisbon Hospital Center Lisbon 1169050Portugal

AIM: To evaluate and compare the quality of life of patients submitted to XEN~? implant or trabeculectomy and the relationship with potentially involved ***: A cross-sectional study of patients with advanced open-angle glaucoma who underwent implantation of XEN~?(group 1) and trabeculectomy(group 2) between October 2015 and February 2017. The studied variables were: age, gender, follow-up time, need of topical anti-hypertensive therapy, visual acuity and intraocular pressure(IOP). The quantification of the quality of life was attained through the Glaucoma Symptom Scale(GSS) questionnaire. RESULTS: Totally 34 eyes(34 patients) were included, 17 in each group. The mean GSS scores for group 1 were 42.6±6.8(median, 47; p25, 36.5; p75, 48.5) and for group 2 it was 41.6±7.0(median, 43; p25, 36.5; p75, 47.0; P=0.34). There was a strong negative correlation between the need for topical anti-hypertensive drugs and the GSS result in both groups(r=-0.88, P<0.01, r=-0.59, P=0.01, respectively) and a moderate negative correlation with IOP in group 1(r=-0.50, P=0.03).CONCLUSION: The analysis demonstrates the noninferiority of medium-term quality of life of one group in relation to the other(XEN~? implant and trabeculectomy). The number of topical anti-hypertensive drugs and IOP negatively influenced the quality of life.

关键词： quality of life XEN^(■) trabeculectomy

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Influence of Inorganic Salts during the Photooxidative Process of Nitrobenzene

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Journal of Environmental Science and Engineering(A) 2013年第4期2卷 267-275页

作者： Fernando Hernandez Aldana Antonio Rivera Miguel Angel Hernandez Eduardo Torres Tonahtiuh Rendon teresa Zayas Leticia flores Chemical Center-Environmental Chemistry BUAP Puebla 72570 Mexico Research Center for Microbial Sciences BUAP Puebla 72570 Mexico Zeolites Research Department BUAP Puebla 72570 Mexico Faculty of Chemical Engineering BUAP Puebla 72570 Mexico

Into the photooxidation process, several factors such as pH, time of irradiation, dose of UV light, lamp power, contaminant concentration, turbidity of the solution and the presence of salts can interfere with the photodegradation of pollutants. This research aims to evaluate the influence of salts： NaCl, MgCl2, CaCI2, BaC12, CuCl2, Na2SO4, MgSO4, MnSO4, FeSO4, CuSO4, Na3PO4, K2CrO4 and K2Cr207 in concentrations of 0.0005 M, 0.005 M and 0.05 M during photodegradation of aqueous solution of 59.5 mg/L of nitrobenzene at a pH of 2.5. It was observed that the presence of salts such as CuCI2, CuSO4, FeSO4, K2CrO4 and K2Cr207 interfere negatively in the system UV/H202 applied for degradation of nitrobenzene; possibly by oxidation of Fe2＋ to Fe3＋ and Cu＋ to Cu2＋ in the Cr case, due to the difficulty of transforming the Cr6＋ to Cr3＋ or because these solutions have color and act as radiation absorbing filter.

关键词： AOPs nitrobenzene photooxidation influence by salts.

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The 2ndStep by Step International Spinal Cord Repair—Combining research Step by Step into multi-pronged approaches for spinal cord repair

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Journal of Biomedical Science and Engineering 2013年第7期6卷 21-32页

作者： maria teresa.moreno-flores Francois Ferón Victor Arvanian Armin Blesch Armin Curt David J. Fink marina Mata Kinichi Nakashima Xavier Navarro Francisco Javier Rodríguez Michal Schwartz Mikael Svensson Erik Sundstrom Joan Romero Filip Lim Department of Biotechnology University Francisco de Vitoria Pozuelo de Alarcón Spain Department of Cell Biology Physiology and Immunology University Autonoma de Barcelona Barcelona Spain Department of Medicine University Hospital Balgrist Zurich Switzerland Department of Molecular Biology University Autónoma de Madrid Madrid Spain Department of Neurobiology Care Sciences and Society Karolinska Institute Stockholm Sweden Department of Neurobiology Weizmann Institute of Science Rehovot Israel Department of Neurology University of Michigan Ann Arbor USA Department of Stem Cell Biology and Medicine Kyushu University Fukuoka Japan Faculty of Medicine Aix Marseille University Marseille France National Paraplegics Hospital Toledo Spain Northport Veterans Affairs Medical Center Northport USA Spinal Cord Injury Center University Hospital Heidelberg Germany Step by Step Foundation Barcelona Spain

On April 26-27, 2013, the Step by Step Foundation hosted the Second International Spinal Cord Repair Meeting at the Fira Barcelona Convention Center in Hospitalet de Llobregat, Spain, highlighting some of the exciting research including clinical trials which show promise for treatments for this devastating disorder. This meeting brought together clinicians, clinical scientists and molecular biologists from more than 10 countries to evaluate current knowledge on clinical, cellular, and biomolecular aspects of spinal cord injury. A major goal of the conference in advancing the translation of research data to the clinic was to promote multi-pronged approaches for therapy of this complex problem.

关键词： Spinal Cord Injury Immunomodulation Glial Scar Nerve Grafts Neurotrophins Neural Stem Cells Electromagnetic Stimulation HSV-1 Vectors Cholecalciferol Vitamin D3 Wnt Proteins Neuroregeneration

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贝伐单抗治疗湿性年龄相关黄斑变性患者SD-OCT形态学变化(英文)

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国际眼科杂志 2017年第3期17卷 399-403页

作者： Manuel Noronha Nuno Moura-Coelho teresa Gomes Rita flores marco Dutra Medeiros 中央里斯本医疗中心眼科葡萄牙里斯本1169-050 新里斯本大学新医学院葡萄牙里斯本1169-050

目的:研究频域光学相干断层成像(spectral domain optical coherence tomography,SD-OCT)定量和定性检测指标在接受玻璃体腔内注射贝伐单抗的年龄相关性黄斑变性(agerelated macular degeneration,AMD)患者人群中的变化,以评估这些指标... 详细信息

目的:研究频域光学相干断层成像(spectral domain optical coherence tomography,SD-OCT)定量和定性检测指标在接受玻璃体腔内注射贝伐单抗的年龄相关性黄斑变性(agerelated macular degeneration,AMD)患者人群中的变化,以评估这些指标是否可以用于预测治疗后视力情况。方法:回顾性分析66眼61例未进行过AMD相关治疗的患者接受至少3mo玻璃体腔内注射贝伐单抗治疗的情况。治疗前后SD-OCT定量检测指标[中央视网膜厚度(central foveal thickness,CFT),外界膜(external limiting membrane,ELM)和椭圆区(ellipsoid zone,EZ)长度]和定性检测指标进行分析和比较。同时,分析这些指标和治疗前后的视力的相关性。结果:平均视力(Log mar)、CFT、ELM和EZ长度治疗前为0.62±0.41、419.3±110.0μm、378.2±377.2μm和156.4±253.7μm,治疗后为0.53±0.44、325.8±117.9μm、547.1±421.5μm和173.1±207.1μm。治疗前视力和CFT(rs=0.27)、ELM长度(rs=-0.30)及ELM断裂(rs=0.43)有相关性。治疗后视力同样和治疗后ELM长度相关(rs=-0.40)。治疗后视力和治疗前视力(rs=0.66)、ELM长度(rs=-0.35)和ELM断裂(rs=0.46)相关。结论:研究显示:治疗前视力、ELM长度和ELM断裂可以用于预测治疗后视力。

关键词：光学相干断层扫描外界膜椭圆区年龄相关性黄斑变性

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Evaluation of Morphological Changes of Aeromonas caviaeSch3 Biofilm Formation under Optimal Conditions

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Advances in Microbiology 2012年第4期2卷 552-560页

作者： Erika Beatriz Angeles-Morales Ricardo Mondragón-flores Juan Pedro Luna-Arias Cinthia teresa Enríquez-Nieto Berenice Parra-Ortega Graciela Castro-Escarpulli Centro de Diagnóstico y Vigilancia Epidemiológica del Distrito Federal. Instituto de Ciencia y Tecnología del Distrito Federal Mexico City Mexico Departamento de Biología Celular Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav-IPN) Mexico City Mexico Departamento de Bioquímica Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav-IPN) Mexico City Mexico Laboratorio de Bacteriología Departamento de Microbiología Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional (ENCB-IPN) Prolongación de Carpio y Plan de Ayala s/n Mexico City Mexico

Aeromonas is a Gram-negative bacterium that lives in aquatic habitats. It can be infective in humans. One of its remarkable attributes is the ability of biofilm formation. Many factors are involved in the construction of biofilms as has been described for Pseudomonas, Klebsiella, and Vibrio, among others. The aim of this work was to study the bacterial morphology during the establishment of biofilm through scanning electron microscopy (SEM) and transmission electron microscopy (TEM) with a modified microtiter plate assay and to determine the best conditions for the establishment of Aeromonas caviae Sch3 biolfilm in vitro. We observed several phenotypic changes, including surface appearance, size, presence of extracellular vesicles from 100 to 250 nm in diameter, and flagella. The best conditions for biofilm formation were to grow cultures at 28℃ at pH 6, as determined by the crystal violet assay. This is, to the best of our knowledge, the first study that describes the cell’s biological events involved in the establishment of biofilm formation of Aeromonas caviae Sch3 in vitro.

关键词： Biofilm Aeromonas Caviae Scanning Electron Microscopy Transmission Electron Microscopy Physical and Chemical Factors

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“Anti-Michael” and Michael Additions in the Reactions of 2-Arylmethyliden-1,3-Indandiones with 2-Aminothiophenol

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International Journal of Organic Chemistry 2017年第1期7卷 57-81页

作者： Jessica J. Sá nchez Garcí a Alberto D. Herná ndez-Suzan Elena martí nez-Klimova marcos flores-Alamo teresa Ramí rez Apan Elena I. Klimova Departamento de Quí mica Orgá nica Universidad Nacional Aut&oacute noma de Mé xico Facultad de Qu&iacute mica Cd. Universitaria Ciudad de M&eacute xico M&eacute xico Instituto de Quí mica Universidad Nacional Aut&oacute noma de Mé xico Facultad de Qu&iacute mica Cd. Universitaria Ciudad de M&eacute xico M&eacute xico

A novel 2-indano[2,3b]-2-ferrocenyl- and 2-indano[2,3b]-2-(p-methoxy-phenyl)[1,5]benzo-2,5-dihydrothiazepine 5a,b (addition Michael/cyclization) (~30.32%), indano[2,3b]-2-ferrocenyl- and 2-(p-methoxyphenyl)[1,4] benzothiazine 4a,b (addition “anti-Michael”/cyclization) (~45.43%), respectively, were obtained by the condensation of 2-ferrocenyl-and 2-(p-methoxy-phenyl)methyliden-1,3-indandiones 1a,b with o-aminothiophenol 2 in the presence of AcOH and HCl. A new “anti-Michael” addition reaction of 1,4-bis-heteronucleophile 2 into 2-arylmethyliden-1,3-indandiones was reported. As a result of this reaction the product 1a,b was obtained. The structures of the resultant compounds were elucidated by IR, 1H and 13C NMR spectroscopy, mass spectrometry, elemental and X-ray diffraction analysis. The in vitro antitumor activity of the obtained products was researched using the following human cancer cell lines: glioblastoma (CNS U-251), prostatic adenocarcinoma (PC-3), chronic myelogenous leukemia (K562), colorectal adenocarcinoma (HCT-15), mammary adenocarcinoma (MCF-7), and small cell lung cancer (SKLU) and the sulforhodamine B (SRB) method. Among these new compounds some thiazine and thiazepine derivatives showed compelling in vitro antitumor effects on cell lines K-562, HCT-15, SKLU-1 and MCF-7.

关键词： Ferrocene Addition “Anti-Michael” Reaction Cyclization 1 5-Thiazepines 4-Thiazines Antitumor Cell Lines

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