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Fast-track protocols in laparoscopic liver surgery:Applicability and correlation with difficulty scoring systems

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World Journal of Gastrointestinal Surgery 2022年第3期14卷 211-220页

作者： Ruben Ciria Ana Padial maría dolores ayllón Carmen García-Gaitan Javier Briceño Unit of Hepatobiliary Surgery and Liver Transplantation University Hospital Reina SofiaCordoba 14004Spain Unit of Anesthesiology University Hospital Reina SofiaCordoba 14004Spain

BACKGROUnD Few series have reported the utility of fast-track protocols(FTP)in minimally invasive liver *** To report the applicability of FTP in minimally invasive liver surgery and to correlate with difficulty *** The series of patients undergoing minimally invasive liver surgery from 2014 was ***,Southampton and Gayet’s scores were compared as predictors of FTP *** of FTP was considered within 24-h,48-h and *** models were performed to define dischargens and *** From 160 cases,78 were candidates for FTP,of which 22(28.2%),19(24.4%)and 14(17.9%)were discharged innd 72-h,respectively(total=71.5%).Iwate,Southampton and Gayet’s scores achieved area under the receiver operating characteristic values fornd 0.698,*** and specificity values for the best score(Iwate)were 87.7%and 66.7%,respectively(cutoff=5.5).In multivariate models,nd complications revealed body mass index as a risk factor independent from difficulty *** The development of aggressive FTP is feasible andn be achieved even in moderate and advanced complexity *** scores,including body mass index value,may be useful to predict which cases may adhere to these protocols.

关键词： Liver Fast-track Enhanced recovery Laparoscopy

Short-term outcomes of robotic liver resection: An initial singleinstitution experience

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World Journal of Hepatology 2022年第1期14卷 224-233页

作者： Manuel Durán Javier Briceño Ana Padial Ferdinando Massimiliano Anelli Juan Manuel Sánchez-Hidalgo maría dolores ayllón Rafael Calleja-Lozano Carmen García-Gaitan Unit of Hepatobiliary Surgery and Liver Transplantation General and Digestive Surgery DepartmentReina Sofia University HospitalCordoba 14004Spain GC18 Translational Research in Surgery of Solid Organ Transplantation Maimonides Biomedical Research InstituteCórdoba 14004Spain GE09 Research in Peritoneal and Retroperitoneal Oncological Surgery Maimonides Biomedical Research InstituteCórdoba 14004Spain Department of Anesthesiology and Resuscitation Reina Sofia University HospitalCordoba 14004Spain

BACKGROUnD Liver surgery has traditionally been characterized by the complexity of its procedures and potentially high rates of morbidity and mortality in inexperienced *** robotic approach has gradually been introduced in liver surgery and has increased notably in recent ***,few centers currently perform robotic liver surgery and experiences in robot-assisted surgical procedures continue to be limited compared to the laparoscopic *** To analyze the outcomes and feasibility of an initial robotic liver program implemented in an experienced laparoscopic hepatobiliary *** A total of forty consecutive patients underwent robotic liver resection(da Vinci Xi,***,United States)between June 2019 and January *** were prospectively followed and retrospectively *** characteristics and perioperative and short-term outcomes were *** are expressed as mean and standard *** study was approved by the Institutional Review *** The mean age of patients was 59.55 years,of which 18(45%)were *** mean body mass index was 29.41 kg/m^(2).nine patients(22.5%)were *** were divided by type of resection as follows:Ten segmentectomies,three wedge resections,ten left lateral sectionectomies,six bisegmentectomies(two VVI bisegmentectomies and four IVb-V bisegmentectomies),two right anterior sectionectomies,five left hepatectomies and two right *** lesions occurred in twenty-nine(72.5%)of the *** mean operative time was 258.11 min and two patients were transfused intraoperatively(5%).Inflow occlusion was used in thirty cases(75%)and the mean total clamping time was 32.62 *** was a single conversion due to uncontrollable *** postoperative complications(Clavien–Dindo>Ⅲb)occurred in three patients(7.5%)and mortality in one(2.5%).no patient required readmission to the *** mean hospital stay was 5.6 *** Althou

关键词： Robotics Hepatectomy Minimally invasive surgery Liver surgery Da vinci

Machine perfusion and the prevention of ischemic type biliary lesions following liver transplant:What is the evidence?

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World Journal of Gastroenterology 2023年第20期29卷 3066-3083页

作者： Manuel Durán Rafael Calleja Angus Hann George Clarke Ruben Ciria Anisa nutu Rebeca Sanabria-Mateos maría dolores ayllón Pedro López-Cillero Hynek Mergental Javier Briceño M Thamara P R Perera Department of Liver Transplantation Reina Sofía University HospitalCórdoba 14004Spain The Liver Unit Queen Elizabeth Hospital BirminghamBirmingham B152THUnited Kingdom Centre for Liver and Gastrointestinal Research Institute of Immunology and ImmunotherapyUniversity of BirminghamBirmingham B152THUnited Kingdom

The widespread uptake of different machine perfusion(MP)strategies for liver transplant has been driven by an effort to minimize graft *** to the cholangiocytes during the liver donation,preservation,or early posttransplant period may result in stricturing of the biliary tree and inadequate biliary *** problem continues to trouble clinicians,and may have catastrophic consequences for the graft and *** injury,as a result of compromised hepatic artery flow,is a well-known cause of biliary strictures,sepsis,and graft ***,very similar lesions can appear with a patent hepatic artery and these are known as ischemic type biliary lesions(ITBL)that are attributed to microcirculatory dysfunction rather than main hepatic arterial *** the warm and cold ischemic period duration appear to influence the onset of *** of the commonly used MP techniques deliver oxygen to the graft cells,and therefore may minimize the cholangiocyte injury and subsequently reduce the incidence of *** clinical experience and published evidence grows for these modalities,the impact they have on ITBL rates is important to *** this review,the evidence for the three commonly used MP strategies(abdominal normothermic regional perfusion[A-nRP],hypothermic oxygenated perfusion[HOPE],and normothermic machine perfusion[nMP])for ITBL prevention has been critically *** with ITBL definitions used in trials,coupled with variations in techniques of MP,make interpretation ***,the evidence suggests that both HOPE and A-nRP prevent ITBL in donated after circulatory death grafts compared to cold *** evidence for ITBL prevention in donor after brain death grafts with any MP technique is weak.

关键词： Liver transplant Ischemic type biliary lesions Hypothermic oxygenated machine perfusion normothermic machine perfusion Abdominal normothermic regional perfusion Donation after circulatory death

Phagocytosis: A Practical Approach for Evaluating neutrophil Function in Health and Disease

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Modern Research in Inflammation 2024年第2期13卷 9-28页

作者： Oscar Rojas-Espinosa Patricia Arce-Paredes Sergio Islas-Trujillo Luis Enrique Becerril-Villanueva maría dolores Ponce-Regalado Departamento de Inmunologa Escuela Nacional de Ciencias Biolgicas Instituto Politcnico Nacional Ciudad de Mxico Mxico Laboratorio de Psicoinmunologa Instituto Nacional de Psiquiatra Ramn de la Fuente Ciudad de Mxico Mxico Departamento de Ciencias de la Salud Centro Universitario de los Altos Universidad de Guadalajara Jalisco Mxico

neutrophils, crucial players in the effector phase of the immune response, are recognized as important mediators of both innate and adaptive immune responses. Through the production of pro- and anti-inflammatory cytokines, they modulate the function of T and other lymphoid cells. Countless reports have highlighted the importance of these cells as efficient antimicrobial agents and annotated their involvement in the pathology of infectious and noninfectious diseases. The development of modern, sophisticated technologies has allowed the study of the functions of these cells in clinical settings. These advanced technologies include fluorescence-activated cell sorters, confocal microscopy, automated cell image analyzers, and live cell analysis instruments. Unfortunately, the cost of these modern instruments, maintenance, reagents, and the need for qualified technicians prohibit their use in low-income laboratories and universities in developing countries. With this in mind, we propose a series of basic tests that can be used in low-input clinical laboratories and universities to evaluate the function of neutrophils in health and disease. Our methodology allows us to assess in a practical and low-cost manner the functions of neutrophils in the phagocytic process, including opsonization, ingestion, ROI production (nBT reduction), myeloperoxidase content, phagosome-lysosome fusion, microbicidal activity, and nET production. Thus, under a disadvantageous ambiance, this may guide physicians in deciding whether a patient’s illness involves phagocytic defects without imposing a heavy financial *** Abstract[-rId13-]

关键词： Phagocytosis neutrophils Microbicidal Activity netosis

Dendritic spine degeneration:a primary mechanism in the aging process

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neural Regeneration Research 2025年第6期20卷 1696-1698页

作者： Gonzalo Flores Leonardo Aguilar-Hernández Fernado García-dolores Humberto nicolini Andrea Judith Vázquez-Hernández Hiram Tendilla-Beltrán Instituto de Fisiología Benemérita Universidad Autónoma de Puebla(BUAP)PueblaMexico Instituto de Ciencias Forenses(InCIFO) Tribunal Superior de Justicia de la Ciudad de México(TSJCDMX).Mexico CityMexico Instituto nacional de Medicina Genómica(InMEGEn) Mexico CityMexico

Recent reports suggest that aging is not solely a physiological process in living beings;instead, it should be considered a pathological process or disease(Amorim et al., 2022). Consequently, this process involves a wide range of factors, spanning from genetic to environmental factors, and even includes the gut microbiome(GM)(Mayer et al., 2022). All these processes coincide at some point in the inflammatory process, oxidative stress, and apoptosis, at different degrees in various organs and systems that constitute a living organism(Mayer et al., 2022;AguilarHernández et al., 2023).

关键词： aging process stress

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Male genitourinary schistosomiasis-related symptoms among long-term Western African migrants in Spain: a prospective population-based screening study

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贫困所致传染病(英文) 2024年第2期13卷 1-10页

作者： Roure Sílvia Vallès Xavier Pérez-Quílez Olga López-Mu?oz Israel Chamorro Anna Abad Elena Valerio Lluís Soldevila Laura Espa?a Sergio Hegazy Alaa H. A. Fernández-Rivas Gema Gorriz Ester Herena dolores Oliveira Mário Miralles maria Carme Conde Carmen Montero-Alia Juan José Fernández-Pedregal Elia Miranda-Sánchez Jose Llibre Josep M. Isnard mar Bonet Josep maria Estrada Oriol Prat núria Clotet Bonaventura The Schisto-Stop study group International Health Program (PROSICS) Barcelona North Metropolitan Territorial Directorate for Infectious Diseases Catalan Institute for Health CAP La Salut Passatge dels Encants S/N Badalona Spain Fundació Lluita contra les Infeccions c/Can Ruti s/n Badalona Spain Infectious Diseases Department Hospital Universitari Germans Trias i Pujol c/Can Ruti s/n Badalona Spain Germans Trias i Pujol Research Institute c/Can Ruti s/n Badalona Spain Microbiology Department Germans Trias i Pujol University Hospital c/Can Ruti s/n Badalona Spain Department of Genetics and Microbiology Universitat Autònoma de Barcelona c/Can Ruti s/n Badalona Spain Faculty of Medicine University of Asyut El Fateh Assiut Governorate Egypt Department of Urology Germans Trias i Pujol University Hospital c/Can Ruti s/n Badalona Spain Canovelles Primary Health Care Unit Barcelona North Metropolitan Health Directorate Catalan Institute for Health C/Indústria Canovelles Spain Primary Health Care Unit Mataró-(Rocafonda-Palau) Barcelona North Metropolitan Health Directorate Catalan Institute for Health Camí Ral el Ravalet Mataró Barcelona Spain Barcelona north Metropolitan Primary Care Directorate Catalan Institute for Health Ctra. de Barcelona Sabadell Barcelona Spain Directorate for Innovation and Interdisciplinary Cooperation Barcelona North Metropolitan Health Directorate Catalan Institute for Health C/Can Ruti S/N Badalona Spain IrsiCaixa-AIDS Research Institute Hospital Universitari Germans Trias i Pujol University Hospital c/Can Ruti s/n Badalona Spain Infectious Diseases Directorate Barcelona North Metropolitan Health Directorate Catalan Institute for Health C/Can Ruti S/N Badalona Spain

Background Schistosomiasis is highly endemic in sub-Saharan Africa and frequently imported to Europe. Male urogenital manifestations are often neglected. We aimed to ascertain the prevalence of genitourinary clinical signs and symptoms among long-term African migrants in a non-endemic European country using a serology *** We carried out a prospective, community-based cross-sectional study of adult male migrants from sub-Saharan Africa living in *** serology tests and microscopic urine examinations were carried out, and clinical data were obtained from an electronic medical record search and a structured *** We included 388 adult males, mean age 43.5 years [Standard Deviation (SD) = 12.0, range: 18–76]. The median time since migration to the European Union was 17 [Interquartile range (IQR): 11–21] years. The most frequent country of origin was Senegal (n = 179, 46.1%). Of the 338, 147 (37.6%) tested positive forSchistosoma. Parasite eggs were present in the urine of only 1.3%. nine genitourinary clinical items were significantly associated with positiveSchistosoma serology results: pelvic pain (45.2%;OR = 1.57, 95%CI: 1.0–2.4), pain on ejaculation (14.5%;OR = 1.85, 95%CI: 1.0–3.5), dyspareunia (12.4%;OR = 2.45, 95%CI: 1.2–5.2), erectile dysfunction (9.5%;OR = 3.10, 95%CI: 1.3–7.6), self-reported episodes of infertility (32.1%;OR = 1.69, 95%CI: 1.0–2.8), haematuria (55.2%;OR = 2.37, 95%CI: 1.5–3.6), dysuria (52.1%;OR = 2.01, 95%CI: 1.3–3.1), undiagnosed syndromic STIs (5.4%), and orchitis (20.7%;OR = 1.81, 95%CI: 1.0–3.1). Clinical signs tended to *** Urogenital clinical signs and symptoms are prevalent among male African long-term migrants with a positiveSchistosoma serology results. Genital involvement can be frequent even among those with long periods of non-residence in their sub-Saharan African countries of origin. Further research is needed to develop diagnostic tools and validate therapeutic approaches to

关键词： Schistosomiasis Chronic schistosomiasis Urogenital schistosomiasis Male genital schistosomiasis Longterm migrant

Functional traits:the pathways to riverine plant resistance in times of hydropeaking

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Ecological Processes 2023年第1期12卷 884-915页

作者： Alejandro Baladrón maría dolores Bejarano Isabel Boavida CERIS Civil Engineering Research and Innovation for SustainabilityInstituto Superior TécnicoUniversidade de LisboaAv.Rovisco Pais1049-001 LisbonPortugal natural Resources Department Universidad Politécnica de Madrid(UPM)Calle JoséAntonio Novais1028040 MadridSpain

Hydropeaking,which refers to rising or falling discharges caused by the turning on or off of hydro-turbines to generate electricity,is a topic of growing interest due to its impact on fluvial *** date,most hydropeaking studies have focused on the impact of peak fluctuations on invertebrate and fish communities,but little attention has been paid to its impact on riverine plants and how functional traits may make them resistant to *** performed a review to assess how a set of 32 plant functional traits can be expressed in riverine plant species,and found evidence of how such expression affects their capacity to cope with common sources of hydropeaking disturbance(i.e.,inundation,fast water drawdown,and rapid water fluctuations linked to up-ramping and down-ramping hydropeaking operations).The information here presented can simplify the evaluation of impacts on riverine plant communities worldwide,as well as to detect suitable species to successfully restore rivers affected by hydropower production.

关键词： Drought Flooding Waterlogging Hydropeaking Plant trait Riverine vegetation

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Vulvar Swelling in a 12-Year-Old Girl: An Early Manifestation of Crohn’s Disease

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Case Reports in Clinical Medicine 2024年第7期13卷 219-226页

作者： Stefania Rendina dolores Ferrara Rosanna Mamone Sara Isoldi Massimo Zeccolini Francesco Esposito Department of Radiology Universit degli studi di Napoli Luigi Vanvitelli Naples Italy Department of Radiology A.O.R.N. Santobono-Pausilipon Children Hospital Naples Italy Pediatric Gastroenterology and Hepatology Unit Santobono-Pausilipon Childrens Hospital Naples Italy

Vulvar Crohn’s disease (VCD) is a rare complication of Crohn’s disease, especially in pediatric population. An early diagnosis can result difficult if the complication does not present in conjunction with the classic gastrointestinal symptoms that characterize this disease. In this study we present the case of a 12-year-old girl whose initial symptom of Crohn’s disease was a symptomatic vulvar swelling promoted by a rectovaginal fistula. We also provide an overview of Crohn’s disease and the vulvar changes found in the course of this disease.

关键词： Recto-Vaginal Fistula Vulvar Swelling Metastatic Crohn’s Disease

Characterization and QTL identification in eggplant introgression lines under two n fertilization levels

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Horticultural Plant Journal(园艺学报:英文版) 2023年第5期9卷 971-985页

作者： Elena Rosa-martínez Gloria Villanueva Ahmet Sahin Pietro Gramazio maría dolores García-martínez maría dolores Raig on Santiago Vilanova Jaime Prohens mariola Plazas Instituto de Conservacion y Mejora de la Agrodiversidad Valenciana Universitat Politecnica de ValenciaCamino de Vera 1446022 ValenciaSpain Instituto de Biología Molecular y Celular de Plantas Consejo Superior de Investigaciones Científicas-Universitat Politecnica de ValenciaCamino de Vera 1446022 ValenciaSpain

Lowering nitrogen inputs is a major goal for sustainable *** the present study,a set of 10 Solanum melongena introgression lines(ILs)developed using Solanum incanum as the exotic donor parent were grown under two nitrogen fertilization doses supplied with the irrigation system:1)8.25 mmol·L-1nH4nO3,corresponding to the high nitrogen treatment(Hn),and 2)no external nitrogen supply,corresponding to the low nitrogen treatment(Ln).Twenty traits,including plant growth and yield parameters,fruit size and morphology,nitrogen and carbon content in leaf and fruit,and phenolics content in fruit,were *** aim was to select of potential materials for eggplant breeding under low n inputs,as well as to identify and locate putative QTLs associated with the traits *** significant differences were observed between the soil characteristics of the Hn and Ln treatments,except for nitrogen and iron content,which was slightly lower in the Hn,probably as a consequence of higher nutrient removal from soil by plants in the latter *** of variance showed that lowering nitrogen inputs did not significantly affect the final yield,fruit morphology,size and phenolics *** agronomic traits were highly and positively correlated with each other under both treatments,as well as total phenolics with chlorogenic acid *** assessment of the differences between each IL and the recipient parent resulted in the identification of 36 QTLs associated with most of the traits—12 were specific to the Hn,17 specific to the Ln,and 7 were stable across *** introgressed fragment of *** generally had a negative effect on the trait,except for QTLs for fruit dry matter,for fruit length on chromosome 10 under the Hn,and for fruit pedicel length on chromosome 9 under the *** increase over An-S-26 of the allele of *** for the QTLs detected ranged between-73.98% and 26.03% in Hn and-73.67%and 34.43% in *** findings provide useful tools fo

关键词： Solanum melongena Solanum incanum Wild relatives n fertilization Abiotic stress QTL

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