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Cotton GhBAK1 Mediates Verticillium Wilt Resistance and Cell Death

Journal of Integrative Plant Biology 2013年第7期55卷 586-596页

作者： Xiquan Gao Fangjun li maoying li Ali S.Kianinejad Jane K.Dever Terry A.Wheeler Zhaohu li Ping He libo Shan Department of Plant Pathology and Microbiology and Institute for Plant Genomics and BiotechnologyTexas A&M UniversityCollege Station College of Agriculture Nanjing Agricultural University College of Agronomy and Biotechnology China Agricultural University Department of Biochemistry and Biophysics and Institute for Plant Genomics and Biotechnology Texas A&M UniversityCollege Station University of Texas at Brownsville Texas A&M Agrilife Research and Extension Center

Virus-induced gene silencing （VIGS） offers a powerful approach for functional analysis of individual genes by knocking down their expression. We have adopted this approach to dissect gene functions in cotton resistant to Verticfllium wilt, one of the most devastating diseases worldwide. We showed here that highly efficient VIGS was obtained in a cotton breeding line （CA4002） with partial resistance to Verticillium wilt, and GhMKK2 and Gh Ve I are required for its resistance to Verticillium wilt. Arabidopsis AtBAK1/SERK3, a central regulator in plant disease resistance, belongs to a subfamily of somatic embryogenesis receptor kinases （SERKs） with five members, AtSERK1 to AtSERK5. Two BAK1 orthologs and one SERK1 ortholog were identified in the cotton genome. Importantly, GhBAK1 is required for CA4002 resistance to Verticillium wilt. Surprisingly, silencing of GhBAK1 is sufficient to trigger cell death accompanied with production of reactive oxygen species in cotton. This result is distinct from Arabidopsis in which AtBAK1 and AtSERK4 play redundant functions in cell death control. Apparently, cotton has only evolved SERK1 and BAK1 whereas AtSERK4/5 are newly evolved genes in Arabidopsis. Our studies indicate the functional importance of BAK1 in Verticillium wilt resistance and suggest the dynamic evolution of SERK family members in different plant species.

关键词： Cell death: Gossvoium hirsutum: Verticillium dahliae: virus-induced qene silence.

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CRISPR/Cas9-mediated mutagenesis of CiBG1 decreased seed size and promoted seed germination in watermelon

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Horticulture Research 2021年第1期8卷 1013-1024页

作者： Yanping Wang Jinfang Wang Shaogui Guo Shouwei Tian Jie Zhang Yi Ren maoying li Guoyi Gong Haiying Zhang Yong Xu National Watermelon and Melon Improvement Center Beijing Academy of Agricultural and Forestry SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)Beijing Key Laboratory of Vegetable Germplasm ImprovementBeijing 100097China

Abscisic acid(ABA)is a critical regulator of seed development and germination.β-glucosidases(BGs)have been suggested to be contributors to increased ABA content because they catalyze the hydrolysis of ABA-glucose ester to release free ***,whether BGs are involved in seed development is *** this study,a candidate gene,CiBG1,in watermelon was selected for targeted mutagenesis via the CRISPR/Cas9 *** size and weight were significantly reduced in the Clbg1-mutant watermelon lines,which was mainly attributed to decreased cell number resulting from decreased ABA levels.A transcriptome analysis showed that the expression of 1015 and 1429 unique genes was changed 10 and 18 days after pollination(DAP),***-and cell cycle-related genes were enriched in the differentially expressed genes of wild type and Clbg1-mutant lines during seed ***,the expression of genes in the major signaling pathways of seed size control was also *** addition,seed germination was promoted in the Cibg1-mutant lines due to decreased ABA *** results indicate that ClBG1 may be critical for watermelon seed size regulation and germination mainly through the modulation of ABA content and thereby the transcriptional regulation of cytoskeleton-,cell cycle-and signaling-related *** results lay a foundation for dissecting the molecular mechanisms of controlling watermelon seed size,a key agricultural trait of significant economic importance.

关键词： CRISPR/Cas9 size thereby

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Natural variation in the NAC transcription factor NONRIPENING contributes to melon fruit ripening

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Journal of Integrative Plant Biology 2022年第7期64卷 1448-1461页

作者： Jinfang Wang Shouwei Tian Yongtao Yu Yi Ren Shaogui Guo Jie Zhang maoying li Haiying Zhang Guoyi Gong Min Wang Yong Xu National Watermelon and Melon Improvement Center Beijing Academy of Agricultural and Forestry SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)Beijing Key Laboratory of Vegetable Germplasm ImprovementBeijing 100097China Sanya Institute Hainan Academy of Agricultural SciencesHaikou 572025China

ThThe NAC transcription factor NONRIPENING(NOR)is a master regulator of climacteric fruit ***(Cucumis melo L.)has climacteric and nonclimacteric fruit ripening varieties and is an ideal model to study fruit *** natural CmNAC-NOR variants,the climacteric haplotype CmNAC-NOR^(S,N) and the non-climacteric haplotype CmNAC-NOR^(A,S),have effects on fruit ripening;however,their regulatory mechanisms have not been ***,we report that a natural mutation in the transcriptional activation domain of CmNAC-NORS,Ncontributes to climacteric melon fruit ***-NOR knockout in the climacteric-type melon cultivar“BYJH”completely inhibited fruit ripening,while ripening was delayed by 5-8 d in heterozygous cmnac-nor mutant *** AC-NOR directly activated carotenoid,ethylene,and abscisic acid biosynthetic genes to promote fruit coloration and ***,CmNAC-NOR mediated the transcription of the“CmNAC-NOR-CmNAC73-CmCWINV2”module to enhance flesh *** transcriptional activation activity of the climacteric haplotype CmNAC-NORS,Non these target genes was significantly higher than that of the nonclimacteric haplotype CmNAC-NOR^(A,S).Moreover,CmNAC-NORS,Ncomplementation fully rescued the non-ripening phenotype of the tomato(Solanum lycopersicum)cr-nor mutant,while CmNAC-NOR^(A,S) did *** results provide insight into the molecular mechanism of climacteric and non-climacteric fruit ripening in melon.

关键词： climacteric fruit fruit ripening melon NAC tran-scription factor

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ClSnRK2.3 negatively regulates watermelon fruit ripening and sugar accumulation

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Journal of Integrative Plant Biology 2023年第10期65卷 2336-2348页

作者： Jinfang Wang Yanping Wang Yongtao Yu Jie Zhang Yi Ren Shouwei Tian maoying li Shengjin liao Shaogui Guo Guoyi Gong Haiying Zhang Yong Xu State Key Laboratory of Vegetable Biobreeding National Engineering Research Center for VegetablesBeijing Key Laboratory of Vegetable Germplasms ImprovementBeijing Vegetable Research CenterBeijing Academy of Agriculture and Forestry ScienceBeijing 100097China

Watermelon(Citrullus lanatus) as non-climacteric fruit is domesticated from the ancestors with inedible fruits. We previously revealed that the abscisic acid(ABA) signaling pathway gene ClSnRK2.3 might infuence watermelon fruit ripening. However,the molecular mechanisms are unclear. Here,we found that the selective variation of ClSnRK2.3 resulted in lower promoter activity and gene expression level in cultivated watermelons than ancestors, which indicated ClSnRK2.3 might be a negative regulator in fruit ripening. Overexpression (OE) of ClSnRK2.3 significantly delayed watermelon fruit ripening and suppressed the accumulation of sucrose, ABA and gibberellin GA4. Furthermore,we determined that the pyrophosphate-dependent phosphofructokinase(ClPFP1) in sugar metabolism pathway and GA biosynthesis enzyme GA20 oxidase(Cl GA20ox) could be phosphorylated by ClSnRK2.3 and thereby resulting in accelerated protein degradation in OE lines and finally led to low levels of sucrose and GA4. Besides that, ClSnRK2.3 phosphorylated homeodomain-leucine zipper protein(ClHAT1) and protected it from degradation to suppress the expression of the ABA biosynthesis gene 9’-cis-epoxycarotenoid dioxygenase 3(Cl NCED3). These results indicated that ClSnRK2.3 negatively regulated watermelon fruit ripening by manipulating the biosynthesis of sucrose, ABA and GA4. Altogether, these findings revealed a novel regulatory mechanism in non-climacteric fruit development and ripening.

关键词： fruit ripening SnRK2.3 sucrose watermelon

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The NAC transcription factor CINAC68 positively regulates sugar content and seed development in watermelon by repressing CIINV and CIGH3.6

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Horticulture Research 2021年第1期8卷 3028-3041页

作者： Jinfang Wang Yanping Wang Jie Zhang Yi Ren maoying li Shaowei Tian Yongtao Yu Yi Zuo Guoyi Gong Haiying Zhang Shaogui Guo Yong Xu National Watermelon and Melon Improvement Center Beijing Academy of Agricultural and Forestry SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)Beijing Key Laboratory of Vegetable Germplasm ImprovementBeijing 100097China

NAC(NAM,ATAF1/2,and CUC2)transcription factors play important roles in fruit ripening and *** watermelon genome encodes 80 NAC genes,and 21 of these NAC genes are highly expressed in both the flesh and vascular *** these genes,CINAC68 expression was signi fi cantly higher in flesh than in ***,the intrinsic regulatory mechanism of CINAC68 in fruit ripening and quality is still *** this study,we found that ClNAC68 is a transcriptional repressor and that the repression domain is located in the *** of CINAC68 by the CRISPR-Cas9 system decreased the soluble solid content and sucrose accumulation in mutant *** was delayed,germination was inhibited,and the IAA content was signi ficantly decreased in mutant *** analysis showed that the invertase gene CUNV was the only gene involved in sucrose metabolism that was upregulated in mutant flesh,and expression of the indole-3-acetic acid-amido synthetase gene CIGH3.6 in the IAA signaling pathway was also induced in mutant *** and dual-luciferase assays showed that CINAC68 directly bound to the promoters of CUNV and CIGH3.6 to repress their *** results indicated that CINAC68 positively regulated sugar and IAA accumulation by repressing CUNV and *** findings provide new insights into the regulatory mechanisms by which NAC transcription factors affect fruit quality and seed development.

关键词： expression metabolism inhibited

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强夯后地基承载力的估算

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岩石力学与工程学报 2006年第Z2期25卷 3523-3528页

作者：曾庆军莫海鸿李茂英华南理工大学建筑学院广东广州510640 广东交通职业技术学院广东广州510650

基于强夯法处理软弱地基承载力的研究现状,对夯后地基承载力作经验统计回归分析,得出强夯后地基容许承载力是单夯击能和点夯击数的函数。研究结果表明,其不仅仅为单夯击能的函数。强夯处理无黏性土地基时,按照拟静力法,首先计算出强夯... 详细信息

基于强夯法处理软弱地基承载力的研究现状,对夯后地基承载力作经验统计回归分析,得出强夯后地基容许承载力是单夯击能和点夯击数的函数。研究结果表明,其不仅仅为单夯击能的函数。强夯处理无黏性土地基时,按照拟静力法,首先计算出强夯拟静力、夯坑深度,然后确定夯后相对密实度。按照前人研究的相对密实度和标准贯入值的经验关系来确定标准贯入值,再依据《建筑地基基础设计规范》(GB50007-2002)的规定,按标准贯入击数确定地基承载力标准值,提出其估算公式。工程实例表明,所获得的公式简单且易应用。强夯后地基承载力的估算可为强夯法处理地基的初步设计和处理方案的比选提供依据。

关键词：土力学强夯无黏性土地基承载力拟静力

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课证岗赛融合的专业课程教学改革与实践

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职业技术教育 2015年第11期36卷 16-19页

作者：李茂英曾庆军广东交通职业技术学院

课证岗赛融合是重要的高职专业课程建设与教学改革模式。以工程造价专业课程建设与教学为案例,围绕学生职业发展能力和专业技能培养,注重融合"造价员考证能力—三大岗位技能—造价技能竞赛能力"要求,建构专业课程体系,编制专业核心课程... 详细信息

课证岗赛融合是重要的高职专业课程建设与教学改革模式。以工程造价专业课程建设与教学为案例,围绕学生职业发展能力和专业技能培养,注重融合"造价员考证能力—三大岗位技能—造价技能竞赛能力"要求,建构专业课程体系,编制专业核心课程标准,开发项目化实践教学资源,在"项目课堂"上实施行动导向教学与考核评价,全程体现课证岗赛融合,全面提升人才培养质量。

关键词：课证岗赛融合工程造价专业专业课程教学改革项目课堂

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镍基析氢阴极技术综述

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广东化工 2015年第7期42卷 114-115,93页

作者：李茂营国家知识产权局专利局专利审查协作江苏中心化学发明审查部江苏苏州215000

氯碱工业是我国基础原材料工业,在国民经济中发挥着举足轻重的作用。降低阴极高析氢过电位是目前氯碱工业节能降耗、提高企业经济效益的关键。在最近十几年中,活性阴极技术的研究与应用日益受到人们的重视。影响镍基析氢活性电极性能的... 详细信息

氯碱工业是我国基础原材料工业,在国民经济中发挥着举足轻重的作用。降低阴极高析氢过电位是目前氯碱工业节能降耗、提高企业经济效益的关键。在最近十几年中,活性阴极技术的研究与应用日益受到人们的重视。影响镍基析氢活性电极性能的主要因素有两种:一是电极材料结晶结构的影响;二为电极真实比表面积的影响,当电极材料的真实表面积远大于表观面积。本文综上了阴极析氢电极在专利申请上的发展历程,从而有助于阴极析氢电极研究人员了解该技术专利动向。

关键词：析氢阴极镍基氯碱

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