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Transcriptome Analysis of Mantle Tissues Reveals Potential Shell-Matrix-Protein Genes in Gigantidas haimaensis

Journal of Ocean University of China 2023年第4期22卷 1087-1097页

作者： SHI Yu YAO Gaoyou he maoxian CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology Guangdong Provincial Key Laboratory of Applied Marine BiologySouth China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou Guangzhou 511458China University of Chinese Academy of Sciences Beijing 100049China

Haima cold seep ecosystem is on the northwestern slope of the South China Sea,which is characterised by high pressure,low temperature,hypoxia,and low pH *** deep-sea mussel Gigantidas haimaensis is distributed in this *** studies have focused on its adaptation to abiotic stress,biogeography,ecotoxicology,genomes,immunity and symbiosis,but knowledge on biomineralisation remains ***,we generated a comprehensive transcript dataset from *** mantle tissue,and 30255 unigenes were *** top 20 most highly expressed genes are related to energy supply,such as mitochondrial genes,suggesting they may mediate the adaptability of this deep-sea mussel to the high pressure and hypoxia *** shell matrix protein(SMP)-related genes were identified from the transcriptome *** PCR analyses showed that five of ten acidic SMPs and nacreous-layer-matrix-protein genes(nacrein,perlucin,perlwapin,pif and mantle protein)were highly expressed in mantle tissue,while the expressions of other five genes(chitinase,SPARC,TRY,papilin and calmodulin)were *** electron microscopy showed that the shell was composed of a prismatic layer and a nacreous layer,and every nacreous layer was made of the whole pieces of aragonite that stacked on top of each *** results indicated the conservation of the structure and functions of nacreous matrix genes in ***,the nacreous layer was made of whole pieces of aragonite that were not quadrilateral or polygonal *** these genes will likely reveal the molecular mechanisms of biomineralisation in *** and other deep-sea mussels.

关键词： Gigantidas haimaensis transcriptome biomineralisation mantle shell structure

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Comparative Transcriptome Analysis of Shell-Matrix-Protein Genes and Observation of the Shell Microstructure in the Deep-Sea Clam Calyptogena marissinica

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Journal of Ocean University of China 2024年第3期23卷 797-810页

作者： SHI Yu LIU Dongting YAO Gaoyou he maoxian CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology Guangdong Provincial Key Laboratory of Applied Marine BiologySouth China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China University of Chinese Academy of Sciences Beijing 100049China

The deep-sea clam Calyptogena marissinica is widely distributed in the Haima cold seep ecosystem on the northwes-tern slope of the South China Sea with low pH values,low temperature and high *** information is available on the biomineralization of this *** this research,we generated a comprehensive transcript dataset of ***’s mantle tissue,and a total of 19821 unigenes were *** shell matrix proteins(SMP)-related genes were *** qPCR results showed that four out of six prismatic matrix genes(MSP2,MSP5,prisilkin-39,and shematrin),four out of the six nacreous matrix genes(perlucin,pif,pif97,and papilin),and two extrapallial fluid proteins(SPARC and calmodulin)were significantly expressed in the *** the nacreous and the prismatic layers are chrysanthemum-shaped,which are stacked on the top of each other to form a laminated nacreous *** alignment and phylogenetic analysis of MSP-5,Prisilkin-39,Perlucin,and Pif homologues showed that some amino acids of *** that differed from those detected in other molluscs may cause the different shape of the nacreous and prismatic layers,but do not lead to a change in the species’evolutionary *** results indicated the conservation of the functions of SMP-related genes in ***,and the specific shape of the prismatic and nacreous layers of this deep-sea mollusc,which will contribute to the research on the molecular regulation mechanisms of biomineralization in *** and provide a new perspective to investigate biomineralization in deep-sea clams in general.

关键词： transcriptome biomineralization mantle tissue shell Calyptogena marissinica

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Transcriptomic Analysis and Comparison of the Gene Expression Profiles in Fast- and Slow-Growing Pearl Oysters Pinctada fucata martensii

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Journal of Ocean University of China 2022年第1期21卷 186-194页

作者： XU Hanzhi LIU Huiru ZHANG Hua he maoxian CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology Guangdong Provincial Key Laboratory of Applied Marine BiologySouth China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China University of Chinese Academy of Sciences Beijing 100049China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China

The pearl oyster Pinctada fucata martensii is an economically valuable shellfish that is cultured for seawater pearl pro-duction,which mainly depends on oyster ***,the growth mechanisms of the pearl oyster are still poorly *** this study,oysters were grouped with relative growth rate,including fast-growing(FG)group and slow-growing(SG)*** Nanopore Technologies(ONT)long-read sequencing was applied to investigate the molecular mechanisms involved in the growth of this *** alternative splicing(AS)types were analyzed in both FG and SG groups,which include alternative 3’splice site,alternative 5’splice site,exon skipping,intron retention,and mutually exclusive *** analysis showed that four of five different AS events(excluding mutually exclusive exons)occurred more frequently in FG than in SG oysters,and the five main AS types exhibited different *** AS events that were detected may be involved in growth,and the difference in ex-pression of AS events between FG and SG oysters may be involved in the mechanism underlying the difference in *** dif-ferentially expressed genes(DEGs)were identified between the FG and SG *** results showed that 40 genes were signifi-cantly up-regulated in FG oysters,while 10 genes were significantly down-regulated in SG *** genes related to nutrient metabolism,shell formation,and immunity were more highly expressed in FG oysters than in SG *** summary,FG oysters exhibited higher metabolic and biomineralization activities and had a more powerful immune system than SG *** results provide insight into the growth of *** that can be used to improve breeding programs.

关键词： Pinctada fucata martensii differential growth Oxford Nanopore Technologies sequencing differentially expressed genes

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Pf-Dmrt4,a potential factor in sexual development in the pearl oyster Pinctada fucata

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Journal of Oceanology and Limnology 2018年第6期36卷 2337-2350页

作者： WANG Qi SHI Yu he maoxian CAS Key Laboratory of Tropical Marine Bio-resources and Ecology Guangdong Provincial Key Laboratory of Applied Marine BiologySouth China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Taiyuan University of Technology Taiyuan 030024China

The mechanisms of sex determination and sex differentiation in the pearl oyster P inctada fucata are currently poorly understood. We therefore investigated the roles of orthologs of the D mrt gene family, key players in male gonad differentiation in mammals, in P. fucata sex diff erentiation and sexual development. Pf-Dmrt4 exhibits features typical of the D mrt family, and displays significant homologies to the DMRT4 cluster. Pf-Dmrt4 mRNA expression in the gonads during a gametogenic cycle, measured by quantitative polymerase chain reaction, was maximal in mature individuals. P f-Dmrt4 expression, demonstrated by in situ hybridization, was localized in the spermatozoa, spermatids, oocytes and vitellogenic oocytes. Knockdown of Pf-Dmrt4 with double-stranded RNA resulted in decreased mRNA expression levels. And Pf-Dmrt4-dsRNA-injected groups showed spawning-stage male gonads, with ruptured follicles and released spermatozoa. Our results enhance the understanding of sex determination and differentiation in P. fucata and suggest that Pf-Dmrt4 could be involved in male gonadal development, and maintenance of male gonadal function.

关键词： Pinctada fucata Pf-Dmrt4 gene expression sexual development

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Effect of Temperature on Gene Expression in the Pearl Oyster Pinctada fucata

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Journal of Ocean University of China 2014年第3期13卷 509-515页

作者： LIU Wenguang HUANG Xie LIN Jianshi he maoxian CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology South China Sea Institute of OceanologyChinese Academy of Sciences

In this study, we examined the effect of elevated temperature on the expression patterns of genes, i.e., nacrein, irr, n16, n19, and hsp70 in the pearl oyster Pinctada fucata. The experiment was carried out at 4 temperatures, i.e., 20℃(ambient, control), 24, 28℃, and 32℃. The expression levels of target genes in P. fucata were assayed at 0, 6, 24, 48, and 96 h via real-time polymerase chain reaction. Results showed that the expression levels of nacrein and irr had no significant variations among different time points below 28℃, but significantly increased over time at 32℃. The expression levels of n16 and n19 did not change markedly at 20℃. The former increased significantly at 6 h and 24 h while the latter substantially decreased during 6–96 h at 24, 28 and 32℃. Among different temperatures, the level of n16 was significantly lower at 20℃ than at other temperatures during 6–96 h, and the level of n19 significantly varied among different temperatures at 48 h and 96 h. The expression level of hsp70 was significantly higher at 32℃ than at 20, 24 and 28℃ at 24 h. These results demonstrated that elevated temperature impacted the physiological processes of P. fucata and potentially influenced its adaptability to thermal stress.

关键词： seawater temperature heat shock protein gene expression pattern Pinctadafucata

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The role of Smad6 in immunity of the pearl oyster Pinctada fucata martensii

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Journal of Oceanology and Limnology 2022年第3期40卷 1135-1147页

作者： Yu SHI Xiaolan PAN Meng XU Huiru LIU Hanzhi XU maoxian he CAS Key Laboratory of Tropical Marine Bio-resources and Ecology Guangdong Provincial Key Laboratory of Applied Marine BiologySouth China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China

Inhibitory Smads(I-Smads),which belong to the Smad family and inhibit bone morphogenic protein 2(BMP2)signaling by a variety of mechanisms,can suppress innate immunity responses in ***,there are no reports for the role of Smad6 in immunity in *** this study,we showed that Smad6 of the pearl oyster Pinctada fucata martensii was located in the Smad6 cluster of the phylogenetic tree;mRNA expression of Smad6 and Smad3 was up-regulated after lipopolysaccharide and polyinosinic:polycytidylic challenge;and transcript levels of Smad6 and Smad3 showed opposite patterns during wound *** salinity stress,water inflow and outflow in the gills appear to be regulated by BMP2-Smads signals,and BMP2-Smads signaling may be closely related to the immune *** results indicate that Smad6 is involved in immunity,that it plays a positive role in the response to immune challenge and an inhibitory role during wound healing,and that Smad6 and Smad3 may work against each other.

关键词： Smad6 BMP2-Smads signal pathway expression immunity Pinctada fucata martensii

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Identification and Expression of the Conotoxin Homologous Genes in the Giant Triton Snail (Charonia tritonis)

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Journal of Ocean University of China 2023年第1期22卷 213-220页

作者： JIA Huixia ZHANG Gege ZHANG Chenglong ZHANG Hua YAO Gaoyou he maoxian LIU Wenguang CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology Guangdong Provincial Key Laboratory of Applied Marine BiologySouth China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China University of Chinese Academy of Sciences Beijing 100049China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China Sansha Marine and Fisheries Bureau Sansha 573199China

Conotoxin(CTX)is a small peptide toxin,and plays crucial role in anesthetizes prey,preys and defends *** tritonis is the natural predator of the crown of thorns starfish(CoTS)and the protector of coral *** plays an important role in the coral reef ***,the types of toxins produced by tritons and the molecular mechanisms of toxin secretion of *** are *** the present study,the four conotoxin homologous genes(CTXs)from *** were *** and conotoxin superfamily phylogenetic tree indicated that CTX-1(***2)and CTX-4(Unigene 58438_All)belong to the C superfamily,CTX-2(Unigene 66414_All)belong to the V superfamily,and CTX-3(Unigene 21408_All)belong to the B1 *** were highly expressed in salivary gland,liver and digestive *** predation control experiment revealed that the expressions of CTXs were significantly different in salivary gland,liver and digestive *** results indicated that CTXs may participate in the process of *** predating CoTs,and provided a scientific basis for further studying the toxins secretion mechanism of ***.

关键词： Charonia tritonis toxin tissue distribution predation experiment

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马氏珠母贝IGF2BP1基因的鉴定及对矿化相关基因表达的影响

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水产学报 2022年第12期46卷 2265-2273页

作者：熊盼盼张华何毛贤中国科学院南海海洋研究所中国科学院热带海洋生物资源与生态重点实验室广东省应用海洋生物学重点实验室南方海洋科学与工程广东省实验室中国科学院大学

为了研究胰岛素样生长因子2结合蛋白(IGF2BPs)基因是否参与珍珠贝的生物矿化，本实验鉴定了一个马氏珠母贝IGF2BP1基因，命名为PfIGF2BP1-1，该基因cDNA全长为7 348 bp,ORF长1 818 bp，编码605个氨基酸。多序列比对和系统进化分析表明，... 详细信息

为了研究胰岛素样生长因子2结合蛋白(IGF2BPs)基因是否参与珍珠贝的生物矿化，本实验鉴定了一个马氏珠母贝IGF2BP1基因，命名为PfIGF2BP1-1，该基因cDNA全长为7 348 bp,ORF长1 818 bp，编码605个氨基酸。多序列比对和系统进化分析表明，PfIGF2BP1-1有2个RRM结构域和4个KH结构域，与其他物种来源的IGF2BPs同源。实时荧光定量PCR结果显示，PfIGF2BP1-1在马氏珠母贝的8个组织中均有表达，在闭壳肌组织中表达量最高，其次为足和外套膜组织。利用pET-32a原核表达载体构建了含有PfIGF2BP1-1成熟多肽区的重组质粒并成功表达蛋白。在IPTG浓度为0.5 mmol/L,37℃培养8 h的条件下诱导表达PfIGF2BP1-1蛋白最佳。PfIGF2BP1-1蛋白刺激马氏珠母贝外套膜原代细胞，引起壳基质蛋白基因Accbp、MSI7、Nacrein的表达水平显著上升，表明PfIGF2BP1-1蛋白可诱导壳矿化相关基因的表达，参与马氏珠母贝的生物矿化过程。

关键词：马氏珠母贝胰岛素样生长因子2结合蛋白1 蛋白表达壳矿化相关基因

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合浦珠母贝实时定量PCR内参基因的稳定性比较

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南方水产科学 2013年第6期9卷 33-40页

作者：王琦何毛贤中国科学院大学北京100039 中国科学院南海海洋研究所中国科学院海洋生物资源可持续利用重点实验室广东广州510301

以合浦珠母贝(Pinctada fucata)为研究对象,应用实时荧光定量PCR技术检测了甘油醛-3-磷酸脱氢酶(GAPDH)、β-肌动蛋白(β-actin)和18S核糖体rRNA(18S rRNA)3个管家基因在合浦珠母贝不同组织、性腺发育时期、胚胎发育时期mRNA水平的表达... 详细信息

以合浦珠母贝(Pinctada fucata)为研究对象,应用实时荧光定量PCR技术检测了甘油醛-3-磷酸脱氢酶(GAPDH)、β-肌动蛋白(β-actin)和18S核糖体rRNA(18S rRNA)3个管家基因在合浦珠母贝不同组织、性腺发育时期、胚胎发育时期mRNA水平的表达情况。同时比较了BestKeeper、geNorm和NormFinde软件对试验数据处理的差异,从中选出合适的内参基因。结果表明β-actin在不同组织和胚胎发育不同阶段表达最稳定,18S rRNA在性腺发育不同时期表达最稳定。

关键词：合浦珠母贝 qPCR 内参基因稳定性分析

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海产经济贝类壳色多态性的研究进展

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海洋通报 2009年第1期28卷 108-114页

作者：管云雁何毛贤中国科学院南海海洋研究所 LMBLAMB广东广州510301 中国科学院研究生院北京100039

海产贝类的贝壳颜色是一个对生产、育种有重要意义的可遗传性状。滨螺、海湾扇贝、皱纹盘鲍、牡蛎、蛤类及珍珠贝等贝类的壳色多态性的遗传研究及壳色选择育种的角度综述了目前海产贝类壳色性状遗传机制的研究进展及壳色多态性在育种中... 详细信息

海产贝类的贝壳颜色是一个对生产、育种有重要意义的可遗传性状。滨螺、海湾扇贝、皱纹盘鲍、牡蛎、蛤类及珍珠贝等贝类的壳色多态性的遗传研究及壳色选择育种的角度综述了目前海产贝类壳色性状遗传机制的研究进展及壳色多态性在育种中的应用,为进一步开展壳色遗传机制和选择育种研究提供理论基础。

关键词：海产贝类壳色多态性壳色变异选育

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