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A COVID-19 T-Cell Response Detection Method Based on a Newly Identified Human CD8+T Cell Epitope from SARS-CoV-2—Hubei Province,China,2021

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China CDC weekly 2022年第5期4卷 83-87,I0005页

作者： Jie Zhang Dan Lu Min li Maoshun Liu Sijia Yao Jianbo Zhan William J.liu George F.Gao NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Savaid Medical School University of Chinese Academy of SciencesBeijingChina College of Laboratory Medicine and life Sciences Wenzhou Medical UniversityWenzhouZhejiangChina Hubei Provincial Center for Disease Control and Prevention WuhanHubeiChina

Introduction:Similar to antibody detection,severe acute respiratory syndrome coronavirus type 2(SARSCoV-2)-specific T-cell response evaluation is also pivotal among the coronavirus disease 2019(COVID-19)convalescents and the vaccinated populations.Nucleocapsid(N)protein is one of the main structural proteins of SARS-CoV-2 and can trigger T-cell responses in humans.Methods:An overlapping peptide pool covering the full length of the N protein was designed,peptides with positive T-cell activating potency in COVID-19 convalescents were screened,and CD8^(+)T cell epitopes were further identified.The epitope was used to detect the SARS-CoV-2-specific CD8^(+)T cell responses in COVID-19 convalescents based in intracellular cytokine staining and tetramer staining in flow cytometry.Results:A human leukocyte antigen A(HLAA)*1101-restricted CD8^(+)T cell epitope,which could stimulate the production of IFN-γvia peripheral blood mononuclear cells(PBMCs)of the convalescents was defined,and the tetramer generated with this epitope could detect SARS-CoV-2-specific T cells in the PBMCs of the convalescents.The structural investigation eliminated that the epitope was a typical HLA-A^(*)1101-restricted T-cell epitope which was conserved among all the sarbecoviruses.Discussion:The newly identified SARS-CoV-2-derived T-cell epitope was helpful to detect the cellular immunity against different sarbecoviruses including SARS-CoV and SARS-CoV-2.This study provided an evaluation method and also a peptide candidate for the research and development of T-cell based vaccine for the virus.

关键词： restricted immunity Human

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In-orbit performance of LE onboard Insight-HXMT in the first 5 years

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Radiation Detection Technology and Methods 2023年第1期7卷 25-33页

作者： Xiaobo li Yong Chen liming Song Weiwei Cui Wei li Juan Wang Shuang-Nan Zhang Fangjun Lu Yupeng Xu Haisheng Zhao Mingyu Ge Youli Tuo Yusa Wang Tianxiang Chen Dawei Han Jia Huo Yanji Yang Maoshun Li Ziliang Zhang Yuxuan Zhu Xiaofan Zhao Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of Science19B Yuquan RoadShijingshan DistrictBeijing 100049China

Purpose The low-energy X-ray telescope(LE)is a main instrument of the Insight-HXMT mission and consists of 96 swept charge devices covering the 1–10 keV energy band.The energy gain and resolution are continuously calibrated by analyzing Cassiopeia A(Cas A)and blank sky data,while the effective areas are also calibrated with the observations of the Crab Nebula.In this paper,we present the evolution of the in-orbit performances of LE in the first 5 years since launch.Methods The Insight-HXMT data analysis software package(HXMTDAS)is utilized to extract the spectra of Cas A,blank sky,and Crab Nebula using different good time interval selections.We fit a model with a power-law continuum and several Gaussian lines to different ranges of Cas A and blank sky spectra to get peak energies of their lines through xspec.After updating the energy gain calibration in CAlibration DataBase(CALDB),we rerun the Cas A data to obtain the energy resolution.An empirical function is used to modify the simulated effective areas so that the background-subtracted spectrum of the Crab Nebula can best match the standard model of the Crab Nebula.Results The energy gain,resolution,and effective areas are calibrated every month.The corresponding calibration results are duly updated in CALDB,which can be downloaded and used for the analysis of Insight-HXMT data.Simultaneous observations with NuSTAR and NICER can also be used to verify our derived results.Conclusion LE is a well-calibrated X-ray telescope working in 1–10 keV band.The uncertainty of LE gain is less than 20eV in 2–9 keV band,and the uncertainty of LE resolution is less than 15eV.The systematic errors of LE,compared to the model of the Crab Nebula,are lower than 1.5%in 1–10 keV.

关键词： X-ray Calibration SCD In-orbit performance

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The genomic characteristics and pathogenicity of a mammalian orthoreovirus within a new lineage from wild pika in plateau

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Virologica Sinica 2023年第6期38卷 877-888页

作者： Kexin Zong Yuanyuan Guo Jingdong Song Maoshun Liu Junfeng Hao Jie Zhang Xin li Shiyan Zhu Shuting Huo Ziqian Xu Peipei liu Yingze Zhao Yuhai Bi Jiapeng Qu George F.Gao Jun liu School of Laboratory Medicine and life Sciences Wenzhou Medical UniversityWenzhou325035China NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention(China CDC)Beijing102206China Laboratory Animal Center Institute of BiophysicsChinese Academy of SciencesBeijing100101China CAS Key Laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing100101China Northwest Institute of Plateau Biology Chinese Academy of SciencesXining810001China Research Unit of Adaptive Evolution and Control of Emerging Viruses(2018RU009) Chinese Academy of Medical SciencesBeijing100021China

Emerging and re-emerging viruses from wild animals have seriously threatened the health of humans and domesticated animals in recent years.Herein,we isolated a new mammalian orthoreovirus(MRV),Pika/MRV/GCCDC7/2019(PMRV-GCCDC7),in the Qinghai-Tibet Plateau wild pika(Ochotona curzoniae).Though the PMRVGCCDC7 shows features of a typical reovirus with ten gene segments arranged in 3:3:4 in length,the virus belongs to an independent evolutionary branch compared to other MRVs based on phylogenetic tree analysis.The results of cellular susceptibility,species tropism,and replication kinetics of PMRV-GCCDC7 indicated the virus could infect four human cell lines(A549,Huh7,HCT,and LoVo)and six non-human cell lines,including Vero-E6,LLCMK2,BHK-21,N2a,MDCK,and RfKT cell,derived from diverse mammals,i.***.monkey,mice,canine and bat,which revealed the potential of PMRV-GCCDC7 to infect a variety of hosts.Infection of BALB/c mice with PMRVGCCDC7 via intranasal inoculation led to relative weight loss,lung tissue damage and inflammation with the increase of virus titer,but no serious respiratory symptoms and death occurred.The characterization of the new reovirus from a plateau-based wild animal has expanded our knowledge of the host range of MRV and provided insight into its risk of trans-species transmission and zoonotic diseases.

关键词： Plateau pika Mammalian orthoreovirus(MRV) Emerging viruses Genomic characteristics Cellular tropism and pathogenicity

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Ionizing radiation effect on 10-bit bipolar A/D converter

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Nuclear Science and Techniques 2010年第3期21卷 152-156页

作者： CHEN Rui LU Wu REN Diyuan ZHENG Yuzhan WANG Yiyuan FEI Wuxiong li maoshun LAN Bo CUI Jiangwei Xinjiang Technical Institute of Physics and Chemistry Chinese Academy of Sciences Urumqi 830011 China Xinjiang Key Laboratory of Electronic Information Material and Device Urumqi 830011 China Graduate University of Chinese Academy of Sciences Beijing 100049 China

In this article,radiation effects and annealing characteristics of a bipolar analog-to-digital converter(ADC) are investigated in different biases and dose rates.The results show that ADC is sensitive to both the bias and dose rate. Under high-dose-rate irradiation,the ADC functions well,while under low-dose-rate irradiation,the parameters of ADC change obviously at low dose level,and the damage is significant at zero bias.Combining the fringing field with the space charge model,the underlying mechanism for this response is discussed.

关键词：数字转换器辐射效应双极性电离辐射低剂量率 ADC 退火特性参数变化

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The Low Energy X-ray telescope (LE) onboard the Insight-HXMT astronomy satellite

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Science China(Physics,Mechanics & Astronomy) 2020年第4期63卷 49-61页

作者： Yong Chen WeiWei Cui Wei li Juan Wang YuPeng Xu FangJun Lul YuSa Wang TianXiang Chen DaWei Han Wei Hu Yi Zhang Jia Huo YanJi Yang Maoshun Li Bo Lu Ziliang Zhang TiPei li ShuangNan Zhang Shaolin Xiong Shu Zhang RongFeng Xue XiaoFan Zhao Yue Zhu YuXuan Zhu HongWei liu YiJung Yang Fan Zhang Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences Beijing 100049China Department of Physics Tsinghua UniversityBeijing 100084China Department of Education Lu Liang UniversityLuliang 033000China Qian Xuesen Laboratory of Space Technology China Academy of Space TechnologyBeijing 100094China College of Physics Jilin UniversityChangchun 130023China

The Low Energy X-ray telescope(LE) is one of the three main instruments of the Insight-Hard X-ray Modulation Telescope(Insight-HXMT). It is equipped with Swept Charge Device(SCD) sensor arrays with a total geometrical area of 384 cm^2 and an energy band from 0.7 to 13 ke V. In order to evaluate the particle induced X-ray background and the cosmic X-ray background simultaneously, LE adopts collimators to define four types of Field Of Views(FOVs), i.e., 1.6°×6°, 4°×6°, 50°-60°×2°-6 oand the blocked ones which block the X-ray by an aluminum cover. LE is constituted of three detector boxes(LEDs) and an electric control box(LEB) and achieves a good energy resolution of 140 e V@5.9 ke V, an excellent time resolution of 0.98 ms, as well as an extremely low pileup(libration on the ground have been performed,including energy-channel relation, energy response, detection efficiency and time response.

关键词： LE X-ray telescope SCD collimators

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水平轴海流能发电机组叶轮模拟研究

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太阳能学报 2016年第8期37卷 2044-2048页

作者：徐全坤石茂顺刘宏伟李伟林勇刚浙江大学流体动力与机电系统国家重点实验室杭州310027

为降低水平轴海流能发电机组海上试验或水槽试验的成本,在实验室内对机组叶轮特性进行模拟。采用叶素动量(BEM)理论对机组的叶轮输出特性进行分析,建立模型机组的Bladed模型,并仿真得到该模型机组的叶轮输出特性。提出基于PLC控制器的... 详细信息

为降低水平轴海流能发电机组海上试验或水槽试验的成本,在实验室内对机组叶轮特性进行模拟。采用叶素动量(BEM)理论对机组的叶轮输出特性进行分析,建立模型机组的Bladed模型,并仿真得到该模型机组的叶轮输出特性。提出基于PLC控制器的异步电机转矩和q轴电流双闭环控制的叶轮模拟方法,搭建模型机组的实验室内试验平台,对模型机组叶轮模拟装置的可行性进行试验验证。试验结果表明,该叶轮模拟装置在机组额定流速以下和额定流速以上均可很好地实现目标转矩的跟踪模拟,为水平轴海流能发电机组的实验室内模拟试验研究提供便利。

关键词：水平轴海流发电叶轮模拟双闭环控制模拟试验

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基层盐政管理长效机制建设浅探

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苏盐科技 2005年第1期 31-32页

作者：李茂顺江苏省盐城市建湖县盐务局江苏建湖224700

盐政管理是党和政府赋予盐业部门的重要职能之一,长期以来深受各级盐务管理部门的高度重视,盐政执法部门始终坚持国家食盐专营方针政策,狠抓盐业市场整治,严厉打击盐业违法犯罪.

关键词：盐政管理部门盐业市场盐务管理盐政执法食盐专营长效机制标的打击保证

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电液数字马达变桨距控制技术

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太阳能学报 2014年第9期35卷 1627-1633页

作者：殷秀兴林勇刚李伟石茂顺楼杉浙江大学流体动力与机电系统国家重点实验室杭州310027

提出一种适用于风力机的电液数字马达变桨距方式,设计电液数字马达变桨距机构及其电液控制系统,构建其数学模型。在此基础上,提出模糊变桨控制策略,采用重复PID变桨控制器,并进行仿真研究。结果验证了该变桨距控制方式的可行性,并表明:... 详细信息

提出一种适用于风力机的电液数字马达变桨距方式,设计电液数字马达变桨距机构及其电液控制系统,构建其数学模型。在此基础上,提出模糊变桨控制策略,采用重复PID变桨控制器,并进行仿真研究。结果验证了该变桨距控制方式的可行性,并表明:该方式易于实现数字式控制,控制实时性和精度较高。

关键词：变桨距控制电液数字马达模糊变桨距策略重复PID控制

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海流能发电系统的负载控制仿真研究

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太阳能学报 2014年第1期35卷 119-124页

作者：石茂顺李伟林勇刚刘宏伟张大海徐全坤浙江大学流体动力与机电系统国家重点实验室杭州310027

基于海流能发电系统最大功率跟踪控制理论,设计以二极管整流-斩波-逆变结构为主电气结构的小功率等级海流能发电系统。系统负载控制器输出PWM信号调节DC/DC变换器,即调节系统负载特性,使得叶轮转速跟踪最优转速,实现系统最大功率跟踪控... 详细信息

基于海流能发电系统最大功率跟踪控制理论,设计以二极管整流-斩波-逆变结构为主电气结构的小功率等级海流能发电系统。系统负载控制器输出PWM信号调节DC/DC变换器,即调节系统负载特性,使得叶轮转速跟踪最优转速,实现系统最大功率跟踪控制运行。在系统数学模型的基础上,搭建Matlab环境下的系统仿真模型并进行仿真分析,仿真结果显示,系统具有良好的动态特性,通过调节负载大小的方法,叶轮实际捕获功率可较好地跟踪理论最大功率,实现系统最大功率输出。

关键词：海流能负载控制最大功率跟踪控制反馈线性化

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海流发电液压传动系统设计及仿真验证

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电力系统自动化 2014年第3期38卷 118-122页

作者：石茂顺刘宏伟李伟林勇刚丁金钟周宏宾流体动力与机电系统国家重点实验室浙江大学浙江省杭州市310027

针对海流发电机组转速控制问题,采用容积调速方法控制离网型海流发电液压传动机组的叶轮转速和发电机转速。设计了两种类似的海流发电液压传动系统方案,均采用泵—马达—发电机直接连接的结构形式。为验证所设计的液压传动系统方案的有... 详细信息

针对海流发电机组转速控制问题,采用容积调速方法控制离网型海流发电液压传动机组的叶轮转速和发电机转速。设计了两种类似的海流发电液压传动系统方案,均采用泵—马达—发电机直接连接的结构形式。为验证所设计的液压传动系统方案的有效性,对其进行数学模型分析,并建立系统的MATLAB/Simulink模型进行系统控制特性仿真研究。结果表明,两种液压传动拓扑结构在满足最大功率跟踪控制要求的前提下,均可以在液压传动系统环节同时实现发电机恒频输出控制。

关键词：海流能液压传动最大功率跟踪恒频控制

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