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Development of automatic and efficient immuno-separator of foodborne pathogenic bacteria using magnetophoresis and magnetic mixing

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International Journal of Agricultural and Biological Engineering 2019年第5期12卷 167-172页

作者： Yuhe wang Gaozhe Cai maohua wang Jianhan Lin Key Laboratory of Agricultural Information Acquisition Technology(Beijing) Ministry of Agriculture and Rural AffairsChina Agricultural UniversityBeijing 100083China Key Laboratory of Modern Precision Agriculture System Integration Research Ministry of EducationChina Agricultural UniversityBeijing 100083China

In recent years,the outbreaks of foodborne diseases caused by pathogenic bacteria have made considerable economic losses and shown a threat to public health.The key to prevent and control these diseases is fast screening of pathogenic bacteria,which is usually performed with three procedures:sample collection,bacteria separation and bacteria detection.For sample collection,the national standard methods are often employed.For bacteria detection,currently available methods such as Polymerase Chain Reaction and Enzyme Linked Immuno-Sorbent Assay are often used.For bacteria separation,traditional methods such as filtration and centrifugation are not capable to specifically separate the target bacteria.However,food samples are very complicated and require efficient pretreatment for bacteria separation and concentration to achieve accurate and reliable results.The conventional immune magnetic separation method can be used to specifically separate the bacteria,but it still cannot meet the requirements for food sample pretreatment due to very low concentration of target bacteria in food.Therefore,this study developed an automatic and efficient immuno-separator of foodborne bacteria based on magnetophoresis and magnetic mixing,and E.coli O157:H7 was used as research model.A magnetic mixer was applied to facilitate the immunoreaction between the immune magnetic nanoparticles and the target bacteria cells,and a magnetophoretic separation tubing was utilized for magnetophoretic separation of the magnetic bacteria.Under the optimal mixing time of 20 min and the optimal flow rate of 50μL/min,the separation efficiency of E.coli O157:H7 could be more than 90%,showing that the developed immuno-separator is promising to be applied for efficient separation of foodborne bacteria and can be easily extended for separation of other biological targets by using their specific antibodies.

关键词： foodborne bacteria immuno-separator bacteria separation magnetophoresis magnetic mixing magnetic nanoparticles

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煤企工业化智能升级改造的经济效益分析

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现代工业经济和信息化 2024年第1期14卷 180-182页

作者：王茂华山西灵石昕益旺岭煤业有限公司山西灵石031300

随着煤矿自动化和信息化建设程度的不断深入,煤企步入了智能化建设的新时代。矿山企业的井下智能集中控制系统可以看作是一个大型的采煤机器人,通过建立统一的数据传输接口和通讯规约,实现与各个子系统的数据通讯,通讯网络将各个相关设... 详细信息

随着煤矿自动化和信息化建设程度的不断深入,煤企步入了智能化建设的新时代。矿山企业的井下智能集中控制系统可以看作是一个大型的采煤机器人,通过建立统一的数据传输接口和通讯规约,实现与各个子系统的数据通讯,通讯网络将各个相关设备联系在一起,满足生产工艺监控要求。以旺岭煤矿10号煤层综采工作面为例,为煤企后工业化时代下智慧矿山的建设提出了建议。对10号煤层综采工作面进行智能化改造,实现了自动化采煤、远程控制和智能监测等。全面提升了矿井安全系数,有效降低了生产经营成本和事故发生率,经济效益十分显著。

关键词：现代化矿井集中控制智能化改造

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Direct Observations of Atmospheric Transport and Stratosphere-Troposphere Exchange from High-Precision Carbon Dioxide and Carbon Monoxide Profile Measurements

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Advances in Atmospheric Sciences 2020年第6期37卷 608-616页

作者： You YI Zhaonan CAI Yi LIU Shuangxi FANG Yuli ZHANG Dongxu YANG Yong wang Miao LIANG maohua wang Institute of Atmospheric Physics Chinese Academy of SciencesBeijing 100029China University of Chinese Academy of Sciences Beijing 100049China Meteorological Observation Centre China Meteorological AdministrationBeijing 100081China Shanghai Advanced Research Institute Chinese Academy of SciencesShanghai 201210China

The balloon-borne Aircore campaign was conducted in Inner Mongolia,China,on June 13 and 142018,which detected carbon dioxide(CO2)and carbon monoxide(CO)profiles from surface to 24 km,showing strong positive and negative correlations between 8 km and 10 km on 13 and 14 June,respectively.Backward trajectories,meteorological analyses,and CO2 horizontal distributions were combined to interpret this phenomenon.The results indicated that the source region experienced a stratospheric intrusion and exhibited a large horizontal CO2 gradient;namely,lower CO concentrations corresponded to higher CO2 concentrations and vice versa.The laminar structure with multiple origins resulted in the highly negative correlation between CO2 and CO in the upper troposphere on 14 June.The contribution of stratospheric air mass to the upper troposphere and that of tropospheric air mass to the lower stratosphere were 26.7%and24.3%,respectively,based on a mass balance approach.Another interesting phenomenon is that CO2 and CO concentrations increased substantially at approximately 8 km on 13 June.An analysis based on the backward trajectory implied that the air mass possibly came from anthropogenic sources.The slope of CO2/CO representing the anthropogenic sources was 87.3 ppm ppm-1.In addition,the CO2 profile showed that there was a large CO2 gradient of 4 ppm km-1 within the boundary layer on 13 June,and this gradient disappeared on 14 June.

关键词： troposphere meteorological intrusion

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生态位的T-S模糊滑模自适应控制

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枣庄学院学报 2024年第2期41卷 21-26,48页

作者：郝云力王茂华阜阳师范大学信息工程学院安徽阜阳236041 阜阳师范大学数学与统计学院安徽阜阳236041

借助生物系统生物个体具有的自适应性,达到智能仿生控制效果。滑膜控制在处理一类具有非线性不确定参数系统问题时具有很强的逼近功能,在T-S模糊滑模控制的基础上,引入生态位贴近度函数作为模糊控制的后件,运用李雅普函数分析系统稳定... 详细信息

借助生物系统生物个体具有的自适应性,达到智能仿生控制效果。滑膜控制在处理一类具有非线性不确定参数系统问题时具有很强的逼近功能,在T-S模糊滑模控制的基础上,引入生态位贴近度函数作为模糊控制的后件,运用李雅普函数分析系统稳定性并求出生态位贴近度函数生态因子的自适应律,反映了生物个体始终朝着自己有利的方向发展,突出本方法的仿生智能控制。

关键词：生态位滑模控制贴近度仿生智能

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A New TanSat XCO2 Global Product towards Climate Studies

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Advances in Atmospheric Sciences 2021年第1期38卷 8-11页

作者： Dongxu YANG Yi LIU Hartmut BOESCH Lu YAO Antonio DI NOIA Zhaonan CAI Naimeng LU Daren LYU maohua wang Jing wang Zengshan YIN Yuquan ZHENG Institute of Atmospheric Physics Chinese Academy of SciencesBeijing 100029China Shanghai Advanced Research Institute Chinese Academy of SciencesShanghai 201210China Earth Observation Science School of Physics and AstronomyUniversity of LeicesterLeicestershire LE17RHUK National Centre for Earth Observation University of LeicesterLeicestershire LE17RHUK National Satellite Meteorological Center China Meteorological Administration Beijing 100081China Shanghai Engineering Center for Microsatellites Shanghai 201210China Changchun Institute of Optics Fine Mechanics and PhysicsChangchun 130033China

The 1st Chinese carbon dioxide(CO2)monitoring satellite mission,TanSat,was launched in 2016.The 1st TanSat global map of CO2 dry-air mixing ratio(XCO2)measurements over land was released as version 1 data product with an accuracy of 2.11 ppmv(parts per million by volume).In this paper,we introduce a new(version 2)TanSat global XCO2 product that is approached by the Institute of Atmospheric Physics Carbon dioxide retrieval Algorithm for Satellite remote sensing(IAPCAS),and the European Space Agency(ESA)Climate Change Initiative plus(CCI+)TanSat XCO2 product by University of Leicester Full Physics(UoL-FP)retrieval algorithm.The correction of the measurement spectrum improves the accuracy(−0.08 ppmv)and precision(1.47 ppmv)of the new retrieval,which provides opportunity for further application in global carbon flux studies in the future.Inter-comparison between the two retrievals indicates a good agreement,with a standard deviation of 1.28 ppmv and a bias of−0.35 ppmv.

关键词： TanSat CO2 remote sensing carbon flux climate change

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A New Global Solar-induced Chlorophyll Fluorescence(SIF)Data Product from TanSat Measurements

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Advances in Atmospheric Sciences 2021年第3期38卷 341-345页

作者： Lu YAO Dongxu YANG Yi LIU Jing wang Liangyun LIU Shanshan DU Zhaonan CAI Naimeng LU Daren LYU maohua wang Zengshan YIN Yuquan ZHENG Key Laboratory of Middle Atmosphere and Global Environment Observation Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China University of Chinese Academy of Sciences Beijing 100049China Shanghai Advanced Research Institute Chinese Academy of SciencesShanghai 201210China Key Laboratory of Digital Earth Science Institute of Remote Sensing and Digital EarthChinese Academy of SciencesBeijing 100094China National Satellite Meteorological Center China Meteorological AdministrationBeijing 100081China Changchun Institute of Optics Fine Mechanics and PhysicsChangchun 130033China Shanghai Engineering Center for Microsatellites Shanghai 201210China

The Chinese Carbon Dioxide Observation Satellite Mission(TanSat)is the third satellite for global CO2 monitoring and is capable of detecting weak solar-induced chlorophyll fluorescence(SIF)signals with its advanced technical characteristics.Based on the Institute of Atmospheric Physics Carbon Dioxide Retrieval Algorithm for Satellite Remote Sensing(IAPCAS)platform,we successfully retrieved the TanSat global SIF product spanning the period of March 2017 to February 2018 with a physically based algorithm.This paper introduces the new TanSat SIF dataset and shows the global seasonal SIF maps.A brief comparison between the IAPCAS TanSat SIF product and the data-driven SVD(singular value decomposition)SIF product is also performed for follow-up algorithm optimization.The comparative results show that there are regional biases between the two SIF datasets and the linear correlations between them are above 0.73 for all seasons.The future SIF data product applications and requirements for SIF space observation are discussed.

关键词： TanSat solar-induced chlorophyll fluorescence retrieval algorithm remote sensing

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塑料对室内和车内环境空气质量的影响

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塑料工业 2024年第3期52卷 20-26页

作者：龚强路少中李国秋李连江刘晓燕王茂华国家室内车内环境及环保产品质量检验检测中心北京101102 长城汽车股份有限公司哈弗技术中心河北保定071000 北京世纪科环生态农业研究院北京101102 国家市场监督管理总局认证认可技术研究中心北京100013

本文分析了塑料产生的各类污染物(微塑料、挥发性有机化合物和气味等)对室内和车内环境带来的危害,分析了现有室内、车内空气质量标准及检测标准。现行标准体系具有局限性,未将塑料带来的有害物质(微塑料和气味等)列入对应标准限量值中... 详细信息

本文分析了塑料产生的各类污染物(微塑料、挥发性有机化合物和气味等)对室内和车内环境带来的危害,分析了现有室内、车内空气质量标准及检测标准。现行标准体系具有局限性,未将塑料带来的有害物质(微塑料和气味等)列入对应标准限量值中,本文提出了塑料在室内和车内空气质量限量值的建议,微塑料≤0.05 mg/m^(3)(重量法)、臭气浓度<10(无量纲)、塑料制品<3级(气味评级),为后续室内车内环境空气质量及塑料制品标准制定和更新提供参考。

关键词：微塑料室内空气质量车内空气质量挥发性有机化合物气味

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CMAES-WFD:Adversarial Website Fingerprinting Defense Based on Covariance Matrix Adaptation Evolution Strategy

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Computers, Materials & Continua 2024年第5期79卷 2253-2276页

作者： Di wang Yuefei Zhu Jinlong Fei maohua Guo School of Cyberspace Security Information Engineering UniversityZhengzhou450000China

Website fingerprinting,also known asWF,is a traffic analysis attack that enables local eavesdroppers to infer a user’s browsing destination,even when using the Tor anonymity network.While advanced attacks based on deep neural network(DNN)can performfeature engineering and attain accuracy rates of over 98%,research has demonstrated thatDNNis vulnerable to adversarial samples.As a result,many researchers have explored using adversarial samples as a defense mechanism against DNN-based WF attacks and have achieved considerable success.However,these methods suffer from high bandwidth overhead or require access to the target model,which is unrealistic.This paper proposes CMAES-WFD,a black-box WF defense based on adversarial samples.The process of generating adversarial examples is transformed into a constrained optimization problem solved by utilizing the Covariance Matrix Adaptation Evolution Strategy(CMAES)optimization algorithm.Perturbations are injected into the local parts of the original traffic to control bandwidth overhead.According to the experiment results,CMAES-WFD was able to significantly decrease the accuracy of Deep Fingerprinting(DF)and VarCnn to below 8.3%and the bandwidth overhead to a maximum of only 14.6%and 20.5%,respectively.Specially,for Automated Website Fingerprinting(AWF)with simple structure,CMAES-WFD reduced the classification accuracy to only 6.7%and the bandwidth overhead to less than 7.4%.Moreover,it was demonstrated that CMAES-WFD was robust against adversarial training to a certain extent.

关键词： Traffic analysis deep neural network adversarial sample Tor website fingerprinting

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激光三维扫描技术在立式搅拌磨螺旋衬板上的应用

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矿山机械 2024年第1期52卷 43-48页

作者：王铭浩李华伟冯浩源姚坤王茂华梁霖浩洛阳矿山机械工程设计研究院有限责任公司河南洛阳471039 智能矿山重型装备全国重点实验室河南洛阳471039

激光三维扫描技术可用于对大型异形件的测量、逆向建模、对比分析。螺旋衬板作为立式搅拌磨更换频率高的易损件,可直接影响磨矿效率、设备运转率。基于此,介绍了激光三维扫描技术在螺旋衬板扫描点云分析处理中的应用;对螺旋衬板的磨损... 详细信息

激光三维扫描技术可用于对大型异形件的测量、逆向建模、对比分析。螺旋衬板作为立式搅拌磨更换频率高的易损件,可直接影响磨矿效率、设备运转率。基于此,介绍了激光三维扫描技术在螺旋衬板扫描点云分析处理中的应用;对螺旋衬板的磨损规律进行探究,建立了衬板损耗模型,并制定了契合用户需求的衬板优化方案;最终实现了对螺旋衬板的结构优化设计,延长了衬板的使用寿命,提高了矿山综合经济效益。

关键词：立式搅拌磨螺旋衬板扫描点云逆向建模磨损规律

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Multiparameter collaborative optimization of the vibrating screen based on the behavior of oil sunflower seed penetrating screen holes

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International Journal of Agricultural and Biological Engineering 2024年第1期17卷 49-58页

作者： Wenbo Wei Jianchang Li Jianjun Hao maohua Xiao Huiju Zhang College of Mechanical and Electrical Engineering Hebei Agricultural UniversityBaoding 071001HebeiChina College of Engineering Nanjing Agricultural UniversityNanjing 210032China The Higher Educational Key Laboratory for Flexible Manufacturing Equipment Integration of Fujian Province Xiamen Institute of TechnologyXiamen 361000FujianChina

The behavior of oil sunflower seeds penetrating screen holes is an important factor that affects the screening performance of oil sunflower seeds.In this study,a double-deck reverse-motion vibrating screening device for oil sunflower seed screening was designed.The force condition and motion law of the oil sunflower seeds on the screen surface were analyzed.This study compared the effect of particle filling amount of discrete element model of oil sunflower seeds on the simulation effects.The screening process was numerically simulated using the coupled Discrete Element Method and Multibody Dynamics(DEM-MBD)technique with the screening percentage of oil sunflower seeds as the index.The influence of the operating parameters of the vibrating screen on the screening effect was analyzed using a multiparameter collaborative optimization scheme.The results of this study can provide a reference for the numerical simulation of crop screening behavior and the development of screening devices.

关键词： oil sunflower seeds screening analysis DEM-MBD discrete element method response surface analysis

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