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Evaluation of biogenic markers-based phenotyping for resistance to Aphanomyces root rot in field pea

Information Processing in Agriculture 2022年第1期9卷 1-10页

作者： Afef Marzougui Abirami Rajendran D.Scott Mattinson Yu Ma Rebecca J.McGee manuel garcia-perez Stephen P.Ficklin Sindhuja Sankaran Department of Biological Systems Engineering Washington State UniversityPullmanWAUnited States Department of Horticulture Washington State UniversityPullmanWAUnited States United States Department of Agriculture-Agricultural Research Service Grain Legume Genetics and Physiology Research UnitWashington State UniversityPullmanWAUnited States

Biogenic volatile organic compounds(VOCs)emitted by plants can reveal information about plant adaptation,defense processes,and biological ***,such VOC data may be utilized to capture phenotypic plant responses to the *** this study,the main objective was to evaluate the potential of biogenic compounds,including VOCs,to phenotype two pea cultivars,Ariel(susceptible)and Hampton(high levels of partial resistance)for resistance to Aphanomyces root rot *** were monitored non-destructively for VOC emission at three-time points(15,20,and 30 days after inoculation,DAI)using dynamic headspace sampling with gas chromatography-flame ionization detec-tion(GC-FID)system,as well as destructively at the end of the experiments,using solvent extraction and pyrolysis of both shoot and root tissues.A non-inoculated control(mock-inoculated with distilled water)was utilized to compare the plant responses within a *** common chemical peaks between control and inoculated samples of both culti-vars(RT_(cm))were analyzed after normalizing the relative peak intensity of inoculated samples with those of control samples,prior to a comparison between *** addi-tion,unique chemical peaks(RT_(uq))present in inoculated samples,but not in control sam-ples were also identified and their relative peak intensities were *** the released green leaf volatiles(RT_(cm)),the normalized relative peak intensity of hexanal emis-sion,at 20 DAI,was higher in Ariel than that of *** addition,several putative chemical peaks(both RT_(cm) and RT_(uq)),previously known as indicators for disease response,exhibited some differences in their emission rates between pea cultivars in at least one of the time *** destructive sampling revealed that shoot samples produced more putative unique biomarkers(RT_(uq))than the root *** on the differences in puta-tive chemical peaks between cultivars,this initial study supports the concept of utilizat

关键词： Plant breeding Pulse crop Volatile organic compounds Plant phenotyping Biotic stress

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A novel elemental composition based prediction model for biochar aromaticity derived from machine learning

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Artificial Intelligence in Agriculture 2021年第1期5卷 133-141页

作者： Hongliang Cao Yaime Jefferson Milan Sohrab Haghighi Mood Michael Ayiania Shu Zhang Xuzhong Gong Electo Eduardo Silva Lora Qiaoxia Yuan manuel garcia-perez Key Laboratory of Agricultural Equipment in Mid-lower Yangtze River College of EngineeringHuazhong Agricultural UniversityNo.1Shizishan StreetHongshan DistrictWuhan 430070PR China Department of Biological Systems Engineering Washington State UniversityPullmanWA 99164USA College of Materials Science and Engineering Nanjing Forestry UniversityNanjing 210037PR China Key Laboratory of Green Process and Engineering National Engineering Laboratory for Hydrometallurgical Cleaner Production TechnologyInstitute of Process EngineeringChinese Academy of SciencesBeijing 100190PR China Excellence Group in Thermal Power and Distributed Generation Federal University of ItajubáBrazil

The measurement of aromaticity in biochars is generally conducted using solid state 13C nuclear magnetic resonance spectroscopy,which is expensive,time-consuming,and only accessible in a small number of researchintensive *** modelling could be a viable alternative to predict biochar aromaticity from other much easier accessible parameters(*** composition).In this research,Genetic Programming(GP),an advancedmachine learning method,is used to develop *** order to identify and evaluate the performance of prediction models,an experimental data set with 98 biochar samples collected from the literature was *** to the benefits of the intelligence iteration and learning of GP algorithm,a kind of underlying exponential relationship between the elemental compositions and the aromaticity of biochars is disclosed *** exponential relationship is clearer and simpler than the polynomial mapping relationships implicated by Maroto-Valer,Mazumdar,and Mazumdar-Wang *** this case,a novel exponential model is proposed for the prediction of biochar *** proposed exponential model appears better prediction accuracy and generalization ability than existing polynomial models during the statistical parameter evaluation.

关键词： Biochar C aromaticity Prediction model Machine learning Genetic programming

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迈向“大分流”:中欧贸易网络和全球消费者在澳门和马赛(18～19世纪)

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海洋史研究 2015年第2期 32-47页

作者：马龙中国人民大学国际关系学院

引言:为什么选择澳门和马赛?近几十年来,全球史已经能与“旧史学”——专注于零散的、地方性的、民族国家内部的历史观相提并论了。“旧史学”过多地使用微历史视角叙述,却没有强调通常定义下地区间的社会经济和文化交流过程。然而,随... 详细信息

引言:为什么选择澳门和马赛?近几十年来,全球史已经能与“旧史学”——专注于零散的、地方性的、民族国家内部的历史观相提并论了。“旧史学”过多地使用微历史视角叙述,却没有强调通常定义下地区间的社会经济和文化交流过程。然而,随着冷战的结束、柏林墙的倒塌,全球史在学术研究上更受关注。通常人们会认为全球史是关于以发达国家现代经济发展为标志的全球化的历史,但事实上全球史是分析人、社会、经济和文化以及全球不同地区的差异的一种研究方法。因此,全球史是一门通过寻找大型问题的答案以帮助人们认知此种全球性进程的交叉科学。彭慕兰(Kenneth Pomeranz)2000年出版的《大分流》是全球(经济)史研究的一个里程碑,它的主要目标是对在工业革命初期发达国家和发展中国家之间的巨大经济差异以及在中国经济发展水平等同甚至超过欧洲的前提下,为什么西欧国家诸如英国和荷兰先一步进行了工业革命,促使它们经济腾飞并领先于中国等问题做出进一步的解释。以加州学派学者为代表的学者们把理论重点放在中国东部地区的经济发展上,诸如彭慕兰、王国斌、杰克·戈德斯通(Jack Goldstone)、李中清、丹尼斯·弗林(Dennis Flynn)、阿图罗·吉拉尔德兹(Arturo Giraldez)、马立博(Robert ***)、万志英(Richard Von Glann)、约翰·霍布森(John Hobson)、杰克·古迪(Jack Goody)、吉姆·布劳特(Jim Blaut)和贡德·弗兰克等知名历史学家。他们通过对“欧洲中心论”这一曾盛行于世界历史的视角发起挑战,分析了西方与东方特别是欧洲和中国之间的巨大经济差异。

关键词：澳门分流消费者经济发展水平发展中国家社会经济网络贸易

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Sperm subpopulations in avian species： a comparative study between the rooster （Gallus domesticus） and Guinea fowl （Numida meleagris）

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Asian Journal of Andrology 2016年第6期18卷 889-894页

作者： manuel garcia-Herreros National Secretariat of Higher Education Science Technology and Innovation (SENESCYT) Quito Ecuador

The main aims of this research were to study possible differences in objective morphometric sperm characteristics, establish normative sperm morphometry standards, and evaluate the presumed different subpopulation distribution of avian spermatozoa from the rooster （Gallus domesticus） and Guinea fowl （Numida meleagris） as model avian species. Seventy-two ejaculates （36 per species studied） were obtained manually, following a training period involving gently combined dorso-abdominal and *** massage of the birds. Ejaculates were processed for volume, sperm concentration, viability, motility, and morphology. Moreover, samples were submitted for sperm morphometric assessment using objective Computer-Assisted Semen Analysis for Morphometry （CASA-Morph） methods, with sperm morphometric descriptors evaluated by Principal Component Analysis （PCA） and multivariate clustering analyses. There were several differences observed between the avian species in values obtained for ejaculate volume and sperm concentration （P 〈 0.001）. Irrespective of species, PCA revealed two Principal Components （PCs） explaining more than 80% of the variance. In addition, the number of subpopulations differed with species （three and five subpopulations for rooster and Guinea fowl, respectively）. Moreover, the distribution of the sperm subpopulations was found to be structurally different between species. In conclusion, our findings from using CASA-Morph methods indicate pronounced sperm morphometric variation between these two avian species. Because of the strong differences observed in morphometric parameter values and their subpopulation distribution, these results suggest that application of objective analytical methods such as CASA-Morph could substantially improve the reliability of comparative studies and help establish valid normative sperm morphological values for avian species.

关键词： Gallus domesticus Numida meleaEris principal component analysis sperm morphometry sperm subpopulations

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The Finite-dimensional Decomposition Property in Non-Archimedean Banach Spaces

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Acta Mathematica Sinica,English Series 2014年第11期30卷 1833-1845页

作者： Albert KUBZDELA Cristina perez-garcia Institute of Civil Engineering Poznań University of TechnologyUl.Piotrowo 561-138 PoznańPoland Department of Mathematics Facultad de CienciasUniversidad de CantabriaAvda.de los Castros s/n39071SantanderSpain

A non-Archimedean Banach space has the orthogonal finite-dimensional decomposition property（OFDDP） if it is the orthogonal direct sum of a sequence of finite-dimensional *** property has an influence in the non-Archimedean Grothendieck＇s approximation theory,where an open problem is the following： Let E be a non-Archimedean Banach space of countable type with the OFDDP and let D be a closed subspace of *** D have the OFDDP？ In this paper we give a negative answer to this question; we construct a Banach space of countable type with the OFDDP having a one-codimensional subspace without the *** we prove that,however,for certain classes of Banach spaces of countable type,the OFDDP is preserved by taking finite-codimensional subspaces.

关键词： Non-Archimedean Banach spaces finite-dimensional decomposition property orthogonal base

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Retinal remodeling following photoreceptor degeneration causes retinal ganglion cell death

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Neural Regeneration Research 2018年第11期13卷 1885-1886页

作者： Diego garcia-Ayuso Johnny Di Pierdomenico Marta Agudo-Barriuso manuel Vidal-Sanz Maria P. ViUegas-perez Departamento de Oftalmologla Facultad de Medicina Universidad de Murcia and Instituto Murciano de Investigacion Biosanitaria Hospital Virgen de la Arrixaca (IMIB-Virgen de la Arrixaca) Murcia Spain

The retina is the extension of the central nervous system that senses *** and rods,situated in the outer retina,convert light into electrical signals that travel through intermediate neurons where these are further pr... 详细信息

关键词： FG Retinal remodeling following photoreceptor degeneration causes retinal ganglion cell death Figure

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Normozoospermic versus teratozoospermic domestic cats： differential testicular volume, sperm morphometry, and subpopulation structure during epididymal maturation

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Asian Journal of Andrology 2016年第6期18卷 871-878页

作者： Miguel Angel Gutierrez-Reinoso manuel garcia-Herreros National Secretariat of Higher Education Science Technology and Innovation (SENESCYT) Quito Ecuador

Teratozoospermia （〈40% morphologically normal spermatozoa/ejaculate） is a frequent phenomenon in feline species. This research was carried out to study the possible differences in testicular volume, differential sperm morphometric traits, and potential differences regarding the sperm subpopulational structure during epididymal sperm maturation in teratozoospermic feline donors. Epididymal sperm samples were collected from the caput （R1）, corpus （R2）, and cauda （R3） epididymidis in two donor groups （N： normozoospermic; T： teratozoospermic）. Aliquots were assessed for concentration, viability, motility, and acrosomal integrity. Sperm morphometric descriptors from CASA-Morph analysis were analyzed by the Principal Component Analysis （PCA） and clustering analyses. Irrespective of the group analyzed, PCA revealed two Principal Components （PCs） for each epididymal region explaining more than the 93% of the variance. Surprisingly, the number of subpopulations remained constant in regions R1-R2-R3 irrespective of the donor group analyzed. However, the distribution of these subpopulations was found to be structurally different and strongly influenced by the epididymal region and the donor group. In conclusion, testicular morphometry and the sperm subpopulation structure were different in N and T donors. The alterations in subpopulations during epididymal maturation could be used as a potential clinical indicator of teratozoospermic individuals since an important influence of teratozoospermia on sperm subpopulation structure has been demonstrated.

关键词： epididymis feline sperm morphometry sperm subpopulations teratozoospermia testicular volume

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Intense pulsed light‑based treatment for the improvement of symptoms in glaucoma patients treated with hypotensive eye drops

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Eye and Vision 2024年第2期10卷 12-21页

作者： Jose Maria Martinez‑de‑la‑Casa Carlos Oribio‑Quinto Almudena Milans‑del‑Bosch Pilar perez‑garcia Laura Morales‑Fernandez Javier garcia‑Bella Jose manuel Benitez‑del‑Castillo Julian garcia‑Feijoo David P.Pinero Ophthalmology Unit Department of Ophthalmology and ORLFaculty of MedicineHospital Clinico San-CarlosUniversidad Complutense de MadridInstituto de Investigacion Sanitaria del Hospital Clinico San-Carlos(IdISSC)28040 MadridSpain Department of Immunology Ophthalmology and ORLSchool of MedicineInstituto de Investigaciones Oftalmologicas Ramon CastroviejoUniversidad Complutense de MadridMadridSpain Department of Optics Pharmacology and AnatomyUniversity of AlicanteAlicanteSpain

Background:Ocular surface disease in glaucoma patients is a significant ocular co-morbidity that can affect 40%to 59%of these patients *** current study was aimed at evaluating the potential clinical benefit of an intense pulsed light(IPL)-based treatment in glaucomatous patients with ocular surface disease due to prolonged hypotensive eyedrop *** our knowledge,this is the first series analyzing the therapeutic effect of this treatment option in this type of ***:This non-comparative prospective case series study enrolled a total of 30 glaucoma patients ranging in age from 57 to 94 years old and treated with hypotensive eyedrops for years with dry eye *** patients received four sessions of IPL treatment using the Optima IPL system(Lumenis,Yokneam,Israel)adjusted to the official optimized Lumenis *** in symptomatology,corneal staining,conjunctival hyperemia,non-invasive break-up time(NIBUT),tear osmolarity,tear meniscus height(TMH),meiboscore and meibomian gland expressibility was analyzed after ***:Statistically significant reductions were observed after IPL treatment in the symptomatology scores measured with different questionnaires[ocular surface disease index(OSDI),standard patient evaluation of eye dryness(SPEED)and symptom assessment questionnaire in dry eye(SANDE)]as well as with the visual analogue scale(P<0.001).Mean change in OSDI was−15.0±11.3.A significant reduction was found after treatment in the corneal staining score(P<0.001).A significant reduction was found in tear film meniscus height(P=0.012),as well as in tear film osmolarity(P=0.001).A significant reduction was also found in meibomian gland expressibility(P=0.003),changing the percentage of grade 3 eyes from 44.4%before IPL to 17.2%after ***:IPL therapy combined with meibomian gland expression(MGX)seems to be an effective option to improve symptomatology in glaucomatous patients with ocular surface diseas

关键词： Glaucoma Intense pulsed light Dry eye Meibomian glands

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2-Cys Peroxiredoxins Participate in the Oxidation of Chloroplast Enzymes in the Dark

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Molecular Plant 2018年第11期11卷 1377-1388页

作者： Valle Ojeda Juan manuel perez-Ruiz Francisco Javier Cejudo Instituto de Bioquimica Vegetaly Fotosintesis Universidad de Sevilla and Consejo Superior de lnvestigaciones CientificasAvenida Americo Vespucio 4941092SevillaSpain

Most redox-regulated chloroplast enzymes are reduced during the day and oxidized during the *** the reduction mechanism of light-dependent enzymes is well known,the mechanism mediating their oxidation in the dark remains *** thiol-dependent peroxidases,2-Cys peroxiredoxins (Prxs),play a key role in light-dependent reduction of chloroplast *** transfer reducing equivalents of thiols to hydrogen peroxide,suggesting the participation of these peroxidases in enzyme oxidation in the ***,we have addressed this issue by analyzing the redox state of well-known redox-regulated chloroplast enzymes in response to darkness in Arabidopsis thaliana mutants deficient in chloroplastlocalized Prxs (2-Cys Prxs A and B,Prx ⅡE,and Prx Q).Mutant plants lacking 2-Cys Prxs A and B,and plants overexpressing NADPH-dependent thioredoxin (Trx) reductase C showed delayed oxidation of chloroplast enzymes in the *** contrast,the deficiencies of Prx ⅡE or Prx Q exerted no *** vitro assays allowed the reconstitution of the pathway of reducing equivalents from reduced fructose 1,6-bisphosphatase to hydrogen peroxide mediated by Trxs and 2-Cys *** together,these results suggest that 2-Cys Prxs participate in the short-term oxidation of chloroplast enzymes in the dark.

关键词： chloroplast enzyme oxidation peroxiredoxin thioredoxin hydrogen peroxide darkness

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Local Phase Portraits through the Newton Diagram of a Vector Field

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Acta Mathematica Sinica,English Series 2015年第6期31卷 1015-1034页

作者： Antonio ALGABA Isabel CHECA Cristóbal garcia manuel REYES Department of Mathematics Faculty of Experimental Sciences

The Newton diagram and, in particular, the lowest-degree quasi-homogeneous terms of an analytic planar vector field allow us to determine the existence of characteristic orbits and separatrices of an isolated singular point. We give an easy algorithm for obtaining the local phase portrait near the origin of a bi-dimensional differential system and we provide several examples.

关键词： Monodromy separatrices phase portraits quasi-homogeneous vector field

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