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New Insights into Plant Isoprenoid Metabolism

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Molecular Plant 2012年第5期5卷 964-967页

作者： Pablo pulido Catalina Perello manuel Rodriguez-Concepcion Department of Molecular Genetics Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB Campus UAB Bellaterra 08193 BarcelonaSpain

Isoprenoids are a hugely diverse family of compounds derived from the C5 precursors isopentenyl diphosphate （IPP） and dimethylallyl diphosphate （DMAPP）. Although all free-living organisms synthesize isoprenoids, they are particularly abun- dant and diverse in plants, with tens of thousands structures known to date. The highest variety of plant isoprenoids are specialized （secondary） metabolites that participate in the interaction of plants with their environment. These include pigments, volatiles, and defense compounds, some of which have applications in industry and agriculture.

关键词： Metabolism Insight Plant

Geochemical characterization of the salinity of irrigated soils in arid regions(Biskra, SE Algeria)

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Acta Geochimica 2021年第2期40卷 234-250页

作者： Mohamed Amine Abdennour Abdelkader Douaoui Jesus Barrena manuel pulido Abdelhamid Bradai Amel Bennacer Chiara Piccini Alberto Alfonso-Torreno Laboratory of Ecosystem Diversity and Agricultural Production System Dynamics in Arid Zones(DEDSPAZA) Mohamed Khider University07000 BiskraAlgeria Laboratory of Crop Production and Sustainable Valorization of Natural Resources Djilali Bounaama University44225 Khemis MilianaAlgeria GeoEnvironmental Research Group University of Extremadura10071 CaceresSpain Laboratory of Water and Environment Hassiba Ben Bouali University02180 ChiefAlgeria Laboratory of Valorization and Conservation of Biological Resources Faculty of SciencesMohamed Bougara University35000 BoumerdesAlgeria Research Centre for Agriculture and Environment Council for Agricultural Research and EconomicsVia della Navicella 2-400184 RomeItaly

The agriculture in Biskra,southeastern Algeria,is based on traditional practices and characterized by small irrigated *** the last decades,the increasing demand in water as well as the scarcity of rainfall has forced many farmers to use groundwater of low quality to maintain the profitability of their ***,this practice seems to be the main harmful factor for soil quality in the region since it is responsible for the salinization of the irrigated *** to assess the impact of this phenomenon,the soils of the irrigated perimeter of El Ghrous-a representative rural community located in the west of Biskra-have been analyzed.A set of 82 soil samples was collected from top and subsoil(0-15 and15-35 cm respectively),on which the following physicochemical analyzes were performed:Ca^(2+),Mg^(2+),K^(+),Na^(+),Cl^(-),SO_(4)^(2-),HCO_(3)^(-),NO_(3)^(-),pH,electrical conductivity(EC)and sodium adsorption ratio.A Principal Component Analysis was performed to individuate the geochemical processes that influenced significantly the evolution of soil salinity and its *** results showed a calcium sulfate(CaSO_(4))facies with a high risk of salinity and a low to medium risk of *** calcite residual alkalinity and generalized residual alkalinity decreased as the solutions became more *** of the samples were oversaturated in carbonate minerals(aragonite,calcite,and dolomite)and under saturated in evaporitic minerals(anhydrite,gypsum,and halite).Finally,two multiple linear regressions(using cations and anions as independent variables)have been proposed to quantify soil *** equations,with an accuracy of 85%,can represent a time and money-saving tool for managers and farmers to estimate the EC,in comparison to the traditional estimation methods.

关键词： Electrical conductivity Cations Anions Soil quality Saturation index PHREEQC

SNOWY COTYLEDON 2 Promotes Chloroplast Development and Has a Role in Leaf Variegation in Both Lotus japonicus and Arabidopsis thaliana

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Molecular Plant 2017年第5期10卷 721-734页

作者： Nicola Zagari Omar Sandoval-lbanez Niels Sandal Junyi Su manuel Rodriguez-Concepcion Jens Stougaard Mathias Pribil Dario Leister Pablo pulido Plant Molecular Biology Department of Biology I Ludwig-Maximilians-Universitat Munchen 82152 Planegg-Martinsried Germany Copenhagen Plant Science Center University of Copenhagen 1871 Frederiksberg C Denmark Research and Innovation Center Fondazione Edmund Mach via E. Mach 1 38010 San Michele all'Adige Italy Centre for Carbohydrate Recognition and Signalling Department of Molecular Biology and Genetics Aarhus University Gustav Wieds Vej 10 8000 Aarhus C Denmark Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB Campus UAB Bellaterra 08193 Barcelona Spain

Plants contain various factors that transiently interact with subunits or intermediates of the thylakoid multiprotein complexes, promoting their stable association and integration. Hence, assembly factors are essential for chloroplast development and the transition from heterotrophic to phototrophic growth. Snowy cotyledon 2 （SCO2） is a DNAJ-like protein involved in thylakoid membrane biogenesis and interacts with the light-harvesting chlorophyll-binding protein LHCBI. In Arabidopsis thaliana, SCO2 function was previ- ously reported to be restricted to cotyledons. Here we show that disruption of SC02 in Lotus japonicus results not only in paler cotyledons but also in variegated true leaves. Furthermore, smaller and pale- green true leaves can also be observed in A. thaliana sco2 （atsco2） mutants under short-day conditions. In both species, SCO2 is required for proper accumulation of PSlI-LHCll complexes. In contrast to other variegated mutants, inhibition of chloroplastic translation strongly affects L. japonicus sco2 mutant devel- opment and fails to suppress their variegated phenotype. Moreover, inactivation of the suppressor of variegation AtClpR1 in the atsco2 background results in an additive double-mutant phenotype with variegated true leaves. Taken together, our results indicate that SCO2 plays a distinct role in PSll assembly or repair and constitutes a novel factor involved in leaf variegation.

关键词： SCO2 DNAJ-like photosynthesis assembly factor variegation chloroplast development

Variables of Suicidal Behavior in Tenerife Years 2011-2012: Proposals for the Prevention

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Forensic Medicine and Anatomy Research 2014年第2期2卷 37-41页

作者： Sergio manuel Martínez Aguilar Milagros De la Rosa Hormiga Juan manuel Herrera Hernández Francisco Rodríguez pulido Candelaria de la Merced Díaz-González Forensic Department Tenerife Spain Servicio Canario de la Salud Tenerife Spain Universidad de La Laguna Tenerife Spain

Suicide is treated by society as a taboo subject despite it being the leading cause of unnatural death in Spain. The Tenerife Province is ranked 16th among the 52 Spanish provinces for its suicide rate. From 2007 till 2010, the rate increased from 2007 to 2008, then dropped in 2010. Slight rise occurred in suicide rates in 2010. This rate increased from 2007 to 2008, with a decrease of rate in 2010. Slight rise occurred in suicide rates in 2010. In 2013 the province saw 75 suicides per year. 90% - 98% of suicides are closely related to mental disease with the remaining 10% associated with different poor causes of the subject, according to bibliography. In this retrospective study, it has been found that the highest risk rests with men over 40 years of age in the central and southern part of the island. Among the forms of suicide reported, the most common is hanging followed by jumping from a height. It’s important to understand the reasons behind suicidal behavior from a psychosocial perspective, taking into account “unsafe” indicators and individual profiles. The lethal nature of a suicide attempt also depends on the form of suicide adopted and the possibility of a rescue. Specific protocols to prevent suicide should be promoted and discussed between health and social services.

关键词： Consummated Suicide Autopsy Violent Death Autopsy Psychosocial

Fast-track program in laparoscopic liver surgery:Theory or fact?

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World Journal of Gastrointestinal Surgery 2012年第11期4卷 246-250页

作者： Belinda Sánchez-Pérez José manuel Aranda-Narváez Miguel Angel Suárez-Muoz Moises elAdel-delFresno José Luis fernández-Aguilar Jose Antonio Pérez-Daga Ysabel pulido-Roa Julio Santoyo-Santoyo Department of General Digestive and Transplantation SurgeryUniversity Regional Hospital Carlos Haya29010 MálagaSpain

AIM:To analyze our results after the introduction of a fast-track(FT) program after laparoscopic liver surgery in our Hepatobiliarypancreatic ***:All patients(43) undergoing laparoscopic liver surgery between March 2004 and March 2010 were included and divided into two consecutive groups:Control group(CG) from March 2004 until December 2006 with traditional perioperative cares(17 patients) and fast-track group(FTG) from January 2007 until March 2010 with FT program cares(26 patients).Primary endpoint was the influence of the program on the postoperative stay,the amount of re-admissions,morbidity and *** we considered duration of surgery,use of drains,conversion to open surgery,intensive cares needs and ***:Both groups were homogeneous in age and *** differences in technique,time of surgery or conversion to open surgery were found,but more malignant diseases were operated in the FTG,and then transfusions were higher in *** and morbidity were similar in both groups,without *** stay was similar,with a median of 3 for CG vs 2.5 for ***,the 80.8% of patients from FTG left the hospital within the first 3 d after surgery(58.8% for CG).CONCLUSION:The introduction of a FT program after laparoscopic liver surgery improves the recovery of patients without increasing complications or re-admissions,which leads to a reduction of the stay and costs.

关键词： Liver surgery Laparoscopy Fast-track

Efficient Techniques and Algorithms for Improving Indoor Localization Precision on WLAN Networks Applications

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International Journal of Communications, Network and System Sciences 2009年第7期2卷 645-651页

作者： Antonio del CORTE-VALIENTE Jose manuel GóMEZ-pulido Oscar GUTIéRREZ-BLANCO 不详

This paper proposes efficient techniques that allow the deploying of high precision location applications for indoor scenarios over Wireless Local Area Networks (WLAN). Firstly, we compare the use of radio frequency (RF) power levels and relative time delays based on ray-tracing as detection methods to estimate the localization of a set of mobile station using the fingerprint technique. Detection method play an important role in applications of high frequencies techniques for locations systems based on current and emerging standards such as Wi-Fi (802.11x) and Wi-Max (802.16x). The localization algorithm computes the Eucli- dean distance between the samples of signals received from each unknown position and each fingerprint stored in the database or radio-map obtained using the FASPRI simulation tool. Experimental results show that more precision can be obtained in the localization process by means of relative delay instead of RF power detection method. Secondly, the Euclidean distance has been compared with others similarity distance measures. Finally, an interpolation algorithm between the fingerprinting weighing based on the distances has been implemented in order to eliminate those fingerprints that do not contribute to the improvement in the accuracy. These techniques allow obtaining more precision in the localization of indoor mobile devices over WLAN networks.

关键词： Localization Euclidean Distance Interpolation Mahalanobis Distance Faspri Fingerprinting GTD

Incidence, risk factors and outcome of de novo tumors in liver transplant recipients focusing on alcoholic cirrhosis

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World Journal of Hepatology 2015年第7期7卷 942-953页

作者： Carlos Jiménez-Romero Iago Justo-Alonso Félix Cambra-Molero Jorge Calvo-pulido álvaro García-Sesma manuel Abradelo-Usera Oscar Caso-Maestro Alejandro Manrique-Municio Service of General and Digestive Surgery Abdominal Organ Transplantation "Doce de Octubre" University Hospital Universidad Complutense de Madrid

Orthotopic liver transplantation(OLT) is an established life-saving procedure for alcoholic cirrhotic(AC) patients, but the incidence of de novo tumors ranges between 2.6% and 15.7% and is significantly increased in comparison with patients who undergo OLT for other etiologies. Tobacco, a known carcinogen, has been reported to be between 52% and 83.3% in AC patients before OLT. Other risk factors that contribute to the development of malignancies are dose-dependent immunosuppression, advanced age, viral infections, sun exposure, and premalignant lesions(inflammatory bowel disease, Barrett's esophagus). A significantly more frequent incidence of upper aerodigestive(UAD) tract, lung, skin, and kidney-bladder tumors has been found in OLT recipients for AC in comparison with other etiologies. Liver transplant recipients who develop de novo non-skin tumors have a decreased long-term survival rate compared with controls. This significantly lower survival rate is more evident in AC recipients who develop UAD tract or lung tumors after OLT mainly because the diagnosis is usually performed at an advanced stage. All transplant candidates, especially AC patients, should be encouraged to cease smoking and alcohol consumption in the pre- and postOLT periods, use skin protection, avoid sun exposure and over-immunosuppression, and have a yearly otopharyngolaryngeal exploration and chest computed tomography scan in order to prevent or reduce the incidence of de novo malignancies. Although still under investigation, substitution of calcineurin inhibitors for sirolimus or everolimus may reduce the incidence of de novo tumors after OLT.

关键词： De novo malignancies De novo tumorstobacco consumption Alcoholic cirrhosis De novocancer Liver transplant

Daptomycin,Methicillin Resistant Staphylococcus hominis Catheter-Related Bacteraemia in a Hemodialysis Patient

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Open Journal of Medical Microbiology 2013年第1期3卷 53-55页

作者： manuel Macia-Heras Javier Donate-Correa Julia Alcoba-Florez Gerardo pulido-Reyes Ernesto Martin-Nunez Joel Martin-Padron Sebastian Mendez-Alvarez Research Unit University Hospital Nuestra Senora de CandelariaSanta Cruz de TenerifeSpain Nephrology Service University Hospital Nuestra Senora de CandelariaSanta Cruz de TenerifeSpain Microbiology Service University Hospital Nuestra Senora de CandelariaSanta Cruz de TenerifeSpain Department of Microbiology and Cell Biology University of La LagunaTenerifeSpain

We report a case of a haemodialysis patient that presented a catheter-related bacteraemia caused by a Coagulase negative Staphylococcus. With the utilization of molecular biology techniques the bacterial isolate recovered from catheter was surprisingly identified as S. hominis by sequencing of the 16S ribosomal gene. The S. hominis isolate, which is not often associated with infections in dialysis patients, was resistant to methicillin, being mecA positive, and to daptomycin. The patient was successfully treated with vancomycin together with the catheter retirement.

关键词： Bacteraemia Catheter Daptomycin Methicillin Staphylococcus hominis

Dendritic cell deficiencies persist seven months after SARS-CoV-2 infection

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Cellular & Molecular Immunology 2021年第9期18卷 2128-2139页

作者： Alberto Pérez-Gómez Joana Vitallé Carmen Gasca-Capote Alicia Gutierrez-Valencia María Trujillo-Rodriguez Ana Serna-Gallego Esperanza Muñoz-Muela María de los Reyes Jiménez-Leon Mohamed Rafii-El-Idrissi Benhnia Inmaculada Rivas-Jeremias Cesar Sotomayor Cristina Roca-Oporto Nuria Espinosa Carmen Infante-Domínguez Juan Carlos Crespo-Rivas Alberto fernández-Villar Alexandre Pérez-González Luis fernando López-Cortés Eva Poveda Ezequiel Ruiz-Mateos JoséMiguel Cisneros Sonsoles Salto-Alejandre Judith Berastegui-Cabrera Pedro Camacho-Martínez Carmen Infante-Domínguez Marta Carretero-Ledesma Juan Carlos Crespo-Rivas Eduardo Márquez Josémanuel Lomas Claudio Bueno Rosario Amaya JoséAntonio Lepe Jerónimo Pachón Elisa Cordero Javier Sánchez-Céspedes manuela Aguilar-Guisado Almudena Aguilera Clara Aguilera Teresa Aldabo-Pallas Verónica Alfaro-Lara Cristina Amodeo Javier Ampuero María Dolores Avilés Maribel Asensio Bosco Barón-Franco Lydia Barrera-pulido Rafael Bellido-Alba Máximo Bernabeu-Wittel Candela Caballero-Eraso Macarena Cabrera Enrique Calderón Jesús Carbajal-Guerrero manuela Cid-Cumplido Yael Corcia-Palomo Juan Delgado Antonio Domínguez-Petit Alejandro Deniz Reginal Dusseck-Brutus Ana Escoresca-Ortega Fátima Espinosa Nuria Espinosa Michelle Espinoza Carmen Ferrándiz-Millón Marta Ferrer Teresa Ferrer Ignacio Gallego-Texeira Rosa Gámez-Mancera Emilio García Horacio García-Delgado manuel García-Gutiérrez María Luisa Gascón-Castillo Aurora González-Estrada Demetrio González Carmen Gómez-González Rocío González-León Carmen Grande-Cabrerizo Sonia Gutiérrez Carlos Hernández-Quiles Inmaculada Concepción Herrera-Melero Marta Herrero-Romero Luis Jara Carlos Jiménez-Juan Silvia Jiménez-Jorge Mercedes Jiménez-Sánchez Julia Lanseros-Tenllado Carmina López Isabel López Álvaro López-Barrios Luis F.López-Cortés Rafael Luque-Márquez Daniel Macías-García Guillermo Martín-Gutiérrez Luis Martín-Villén JoséMolina Aurora Morillo María Dolores Navarro-Amuedo Dolores Nieto-Martín Francisco Ortega María Paniagua-García Amelia Peña-Rodríguez E Clinical Unit of Infectious Diseases Microbiology and Preventive MedicineInstitute of Biomedicine of Seville(IBiS)Virgen del Rocío University HospitalCSICUniversity of SevilleSevilleSpain Department of Medical Biochemistry Molecular Biologyand ImmunologySchool of MedicineUniversity of SevilleSevilleSpain Pneumology Service Galicia Sur Health Research Instituto(IIS Galicia Sur)SERGAS-UVigoVigoSpain Infectious Diseases Unit Department of Internal MedicineComplexo HospitalarioUniversitario de VigoIIS Galicia SurSERGAS-UVigoVigoSpain Group of Virology and Pathogenesis Galicia Sur Health Research Institute(IIS Galicia Sur)-Complexo Hospitalario Universitario de VigoSERGAS-UVigoVigoSpain Unit of Infectious Diseases Microbiologyand Preventive MedicineVirgen del Rocío University HospitalSevilleSpain Institute of Biomedicine of Seville(IBiS) Virgen del Rocío University Hospital/CSIC/University of SevilleSevilleSpain Medico-Surgical Unit of Respiratory Diseases Virgen del Rocío University HospitalSevilleSpain Unit of Emergencies Virgen del Rocío University HospitalSevilleSpain Intensive Care Unit Virgen del Rocío University HospitalSevilleSpain Department of Medicine University of SevilleSevilleSpain Cardiology and Cardiovascular Surgery Unit Virgen del Rocío University HospitalSevilleSpain Rheumatology Unit Virgen del Rocío University HospitalSevilleSpain Internal Medicine Unit Virgen del Rocío University HospitalSevilleSpain Digestive and Hepatobiliary Unit Virgen del Rocío University HospitalSevilleSpain Neurology and Clinical Neurophysiology Unit Virgen del Rocío University HospitalSevilleSpain Endocrinology and Nutrition Unit Virgen del Rocío University HospitalSevilleSpain Urology and Nephrology Unit Virgen del Rocío University HospitalSevilleSpain Unit of Clinical Investigation and Clinical Trials Virgen del Rocío University HospitalSevilleSpain

Severe Acute Respiratory Syndrome Coronavirus(SARS-CoV)-2 infection induces an exacerbated inflammation driven by innate immunity *** cells(DCs)play a key role in the defense against viral infections,for instance plasmacytoid DCs(pDCs),have the capacity to produce vast amounts of interferon-alpha(IFN-α).In COVID-19 there is a deficit in DC numbers and IFN-αproduction,which has been associated with disease *** this work,we described that in addition to the DC deficiency,several DC activation and homing markers were altered in acute COVID-19 patients,which were associated with multiple inflammatory ***,previously hospitalized and nonhospitalized patients remained with decreased numbers of CD1c+myeloid DCs and pDCs seven months after SARS-CoV-2 ***,the expression of DC markers such as CD86 and CD4 were only restored in previously nonhospitalized patients,while no restoration of integrinβ7 and indoleamine 2,3-dyoxigenase(IDO)levels were *** findings contribute to a better understanding of the immunological sequelae of COVID-19.

关键词： SARS-CoV-2 COVID-19 Dendritic cell Long-COVID