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Journal of Renewable Materials 2022年第10期10卷 2543-2552页

作者： Vianey Mendez-Trujillo Benjamín Valdez-Salas Mario Curiel-Alvarez Ernesto Beltran-Partida Arnoldo Alfaro-Corres EsaúRuiz-sanchez Gerardo Bautista-Trujillo Olivia Tzintzun Camacho Onecimo Grimaldo-Juarez Carlos Ceceña-duran Carlos Ail-Catzin Daniel González-Mendoza Facultad de Medicina de la Universidad Autónoma de Baja California Dr.Humberto Torres Sanginés S/NCentro CívicoMexicaliBaja CaliforniaMéxico Instituto de Ingeniería de la Universidad Autónoma de Baja California Calle de la Normal S/N y Boulevard Benito JuárezMexicaliBaja CaliforniaMéxico Instituto Tecnológico de Conkal División de Estudios de Posgrado e InvestigaciónAntigua Carretera Mérida-MotulConkalYucatánMéxico Facultad de Medicina Veterinaria y Zootecnia de la Universidad Autónoma de Chiapas ChiapasMéxico Universidad Autónoma de Baja California Instituto de Ciencias AgrícolasBaja CaliforniaMéxico

The present study was conducted to evaluate the impact of bimetallic nanoparticles(NPs)of copper(Cu)and zinc(Zn)from Crotalaria longirostrata on the insect mortality and cell viability of Phenacoccus *** biosynthesis of bimetallic nanoparticles(Cu/Zn-NPs)from *** was confirmed by the presence of a single peak around 378 *** hydrodynamic diameter of the Cu/Zn nanoparticles varied from 59 to 119 nm and their zeta potential was found to be−30.90 *** EDX analysis showed a strong signal for Cu and Zn(8 and 8.6 keV,respectively).The Cu/Zn-NPs showed an effective insecticide activity against *** with 63%of insect mortality after 96 h ***,further studies about nanotoxicity of Cu/Zn-NPs from *** are needed to reveal the exact mechanism of nanoparticles toxicity in insects.

关键词： Phytonanoparticles chipilin biocontrol agronanotechnology

Proteomic analysis for developing new biomarkers of hepatocellular carcinoma

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World Journal of Hepatology 2010年第3期2卷 127-135页

作者： Maria Pleguezuelo Laura M Lopez-sanchez Antonio Rodriguez-Ariza Jose L Montero Javier Briceno Ruben Ciria Jordi Muntane manuel de la Mata Liver Research Unit and Academic Department of Surgery Reina Sofia University Hospital Research Unit Reina Sofia University Hospital Liver Research Unit and Academic Department of Surgery CIBERehd Reina Sofia University Hospital

AIM: To identify new markers of hepatocellular carcinoma (HCC) using a proteomic analysis. METHODS: Patients with liver cirrhosis of the three most frequent etiologies: hepatitis C virus, hepatitis B virus and alcoholic liver disease, were included in the study. The samples were analysed by 2D-electrophoresis in order to determine the differential protein expression. The proteins were separated according to the charge in immobilized pH 3-10 gradient strips and then by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Proteins of interest were excised, digested with trypsin and the resulting peptides were separated and identified. RESULTS: Three differentially expressed apolipoproteins (Apo) were identified based on the protein profile using proteomic techniques: Apo-A1, Apo-A4 and Apo-E. Apo-A4 levels were significantly lower in HCC than in non-HCC patients regardless of etiology (P < 0.01). Multivariate logistic regression showed that Apo-A4 and Apo-A1 were the only independent factors related to HCC diagnosis (P < 0.05). The receiver operating characteristic (ROC) curve including both Apo-A4 and Apo-A1 showed an area under the ROC of 0.944 (P < 0.001), a sensitivity of 0.89 and a specificity of 0.81 for diagnosis of HCC. CONCLUSION: Apo-A4 and Apo-A1 may be used clinically as biomarkers of HCC with a high sensibility and specificity. These findings may provide additional insights into the mechanism of HCC development and progression.

关键词： Liver cancer Apolipoproteins Serum biomarkers 2D polyacrylamide gel electrophoresis Mass spectrometry

How Does the Mind Handle Uncertainty in Ambiguous Figures？

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Psychology Research 2016年第1期6卷 1-13页

作者： manuel Moreno-sanchez J. Antonio Aznar-Casanova Nelson Torro-Alves University of Barcelona Barcelona Spain Institute for Brain Cognition &Behavior (IR3C) University of Barcelona Barcelona Spain Federal University of Paraiba Paraiba Brazil

Strengthening Eating Habits through a Communication Program, Case Study： Colegio Inter Canadiense de Puebla

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通讯和计算机（中英文版） 2011年第11期8卷 988-996页

作者： Jesus Roberto sanchez Reina Hilda Gabriela Hernandez Flores Jose manuel Ramos Rodriguez Universidad lnteramericana para el desarrollo Campus Atlixco Puebla 72000 Mexico Communications Science Faculty Benemdrita Universidad Aut6noma de Puebla Puebla 72000 Mexico Faculty of Communication. Autonomous University of Puebla Mexico Puebla CP 72800 Mexico

Validation of a preclinical dry eye model in New Zealand white rabbits during and following topical instillation of 1% ophthalmic atropine sulfate

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Animal Models and Experimental Medicine 2022年第3期5卷 266-273页

作者： Alejandra Sánchez-Ríos Elba Yadira Correa-Gallegos José manuel Medina-Espinoza Andrea Anaid Navarro-sanchez Oscar Olvera-Montaño Leopoldo Baiza-Durán Patricia Muñoz-Villegas Medical Affairs Department Laboratorios SophiaSA de CVZapopanJaliscoMexico

Background : The objective of this study was to validate an animal model for dry eye during and after the administration of 1% ophthalmic atropine sulfate(OAS) in New Zealand white(NZW) *** : OAS(1%) was applied three times per day to 30 eyes of 15 healthy NZW rabbits. Sacrifice, enucleation, and lacrimal gland removal took place on days 15, 21,and 30(OAS group). A second group(n = 5) was used as control. Clinical evaluations took place on days 3, 10, 15, 18, 21, 24 and 30. The primary endpoints were:Schirmer I test, tear break-up time(TBUT), and corneal fluorescein staining. As secondary endpoints, clinical changes including intraocular pressure, and histopathology were *** : While OAS was administered, the Schirmer I test showed a statistically significant reduction for OAS group versus control( p < 0.001), and versus basal production( p < 0.001). TBUT showed statistically significant differences between groups(days 3 and 10;p = 0.001) and versus basal values(day 3;p < 0.001). Fluorescein staining showed a statistically significant difference(day 3;p = 0.001). The most frequent clinical finding was conjunctival hyperemia(76.9% OAS vs. 20% control). For histopathology, all OAS subjects presented some degree of inflammation(86.7% minimal;13.3% mild) whereas the control presented only 30% minimal inflammation. Goblet cell density showed no *** : The effectiveness of the OAS dry eye model in NZW rabbits as reported in previous studies was confirmed, provided that the application of the drug is maintained throughout the intervention;it is not a viable model after OAS administration is suspended.

关键词： atropine sulfate dry eye rabbit model safety Schirmer I test TBUT

Simulation, Construction and Characterization of a Piezoelectric Transducer Using Rexolite as Acoustic Matching

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材料科学与工程（中英文B版） 2013年第4期3卷 208-213页

作者： Israel sanchez Dominguez Pedro Acevedo Contla manuel Recuero Lopez Demetrio Fabian Garcia Nocetti Polytechnic University of Madrid School of Industrial Engineering Madrid Spain National Autonomous University of Mexico lnstitutito Research in Applied Mathematics and Systems Mexico City Mexico

From advanced diagnosis to advanced resection in early neoplastic colorectal lesions:Never-ending and trending topics in the 2020s

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World Journal of Gastrointestinal Surgery 2022年第7期14卷 632-655页

作者： Francesco Auriemma Sandro Sferrazza Mario Bianchetti Maria Flavia Savarese Laura Lamonaca Danilo Paduano Nicole Piazza Enrica Giuffrida Lupe sanchez Mete Alessandra Tucci Sebastian manuel Milluzzo Chiara Iannelli Alessandro Repici Benedetto Mangiavillano Gastrointestinal Endoscopy Unit Humanitas Mater DominiCastellanza 21053Italy Gastroenterology and Endoscopy Unit Santa Chiara HospitalTrento 38014Italy Digestive Endoscopy Unit San Giuseppe Hospital-MultimedicaMilan 20123Italy Department of Gastroenterology and Gastrointestinal Endoscopy General HospitalSanremo 18038Italy Gastroenterology Unit IRCCS Policlinico San DonatoSan Donato MilaneseDepartment of Biomedical Sciences for HealthUniversity of MilanMilan 20122Italy Gastroenterology and Hepatology Unit A.O.U.Policlinico"G.Giaccone"Palermo 90127Italy Department of Gastroenterology and Digestive Endoscopy IRCCS Regina Elena National Cancer InstituteRome 00144Italy Department of Gastroenterology Molinette HospitalCittàdella salute e della Scienza di TorinoTurin 10126Italy Digestive Endoscopy Unit and Gastroenterology Fondazione PoliambulanzaBrescia 25100Italy Department of Health Sciences Magna Graecia UniversityCatanzaro 88100Italy Digestive Endoscopy Unit and Gastroenterology Humanitas Clinical and Research Center and Humanitas UniversityRozzano 20089Italy Biomedical Science HunimedPieve Emanuele 20090Italy Gastrointestinal Endoscopy Unit Humanitas Mater DominiCastellanzaVarese 21053Italy

Colonoscopy represents the most widespread and effective tool for the prevention and treatment of early stage preneoplastic and neoplastic lesions in the panorama of cancer *** the world there are different approaches to the topic of colorectal cancer prevention and screening:different starting ages(45-50 years);different initial screening tools such as fecal occult blood with immunohistochemical or immune-enzymatic tests;recto-sigmoidoscopy;and *** key aspects of this scenario are composed of a proper bowel preparation that ensures a valid diagnostic examination,experienced endoscopist in detection of preneoplastic and early neoplastic lesions and open-minded to upcoming artificial intelligence-aided examination,knowledge in the field of resection of these lesions(from cold-snaring,through endoscopic mucosal resection and endoscopic submucosal dissection,up to advanced tools),and management of complications.

关键词： Colorectal lesions Colorectal tumor Endoscopic submucosal dissection Endoscopic mucosal resection Cold-endoscopic mucosal resection FTRD® Complications Adverse events Polypectomy

Purification and Biochemical Characterization of a Protease Inhibitor Ⅱ Family from Jalapeno Pepper(Capsicum annuum L.)

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Advances in Bioscience and Biotechnology 2014年第7期5卷 661-668页

作者： Juan Pablo Carrillo-Montes Roberto Arreguin-Espinosa Jose Luis Munoz-sanchez manuel Soriano-Garcia Departamento de Quimica de Biomacromoleculas Instituto de QuimicaUniversidad Nacional Autonoma de MexicoMexico DFMexico Departamento de Bioquimica Escuela Nacional de Ciencias Biologicas del Instituto Politécnico NacionalMéxico DFMéxico

Capsicum annuum L. was initially domesticated in Mexico and northern Central America, and represented an ancient Neotropical plant food complex. The purpose of this paper is to report the isolation and purification of a novo-member of a protease inhibitor from jalapeño pepper (Capsicum annuum L.) (PIJP). The molecular weight of PIJP inhibitor is 5.95 kDa with 56 amino acids and 6 Cys residues with high inhibitory activity to trypsin with a Ki value of 95 nM. This inhibitor according to the alignment with homologous from NCBI and Pfam databases is a member of proteinase inhibitors II. It is worthwhile to mention a major compositional difference between the proteinase inhibitor II families which have 8 Cys residues. PIJP is the first purified proteinase inhibitor, member of this family with only 6 Cys residues.

关键词： Protease Inhibitory Activity Protease Inhibitor Protein Purification Jalapeno Pepper Capsicum annuum L.

Gut Microbiota in Metabolic-associated Fatty Liver Disease and in Other Chronic Metabolic Diseases

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Journal of Clinical and Translational Hepatology 2021年第2期9卷 227-238页

作者： Winston Hernández-Ceballos Jacqueline Cordova-Gallardo Nahum Mendez-sanchez Plan of Combined Studies in Medicine(PECEM-MD/PhD) Faculty of MedicineNational Autonomous University of MexicoMexico CityMexico Department of Hepatology Service of Surgery and Obesity ClinicGeneral Hospital“Dr.Manuel Gea González”Mexico CityMexico Faculty of Medicine.National Autonomous University of Mexico Mexico CityMexico Liver Research Unit Medica Sur Clinic&FoundationMexico CityMexico

The gut microbiome plays a key role in the health-disease balance in the human *** its composition is unique for each person and tends to remain stable throughout lifetime,it has been shown that certain bacterial patterns may be determining factors in the onset of certain chronic metabolic diseases,such as type 2 diabetes mellitus(T2DM),obesity,metabolic-associated fatty liver disease(MAFLD),and metabolic *** gut-liver axis embodies the close relationship between the gut and the liver;disturbance of the normal gut microbiota,also known as dysbiosis,may lead to a cascade of mechanisms that modify the epithelial properties and facilitate bacterial *** of gut microbiota is fundamental to maintaining gut integrity,as well as the bile acids *** the present review,we summarize the current knowledge regarding the microbiota,bile acids composition and their association with MAFLD,obesity,T2DM and metabolic syndrome.

关键词： Metabolic-associated fatty liver disease Metabolic-associated steatohepatitis Gut microbiota Gut-liver axis Dysbiosis Bile acids

Fiber laser with simultaneous multi-wavelength Er/Yb passively Q-switched and single-wavelength Tm gain-switched operations

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Photonics Research 2019年第6期7卷 608-614页

作者： J.ALANIZ-BAYLON M.duran-sanchez R.I.ALVAREZ-TAMAYO B.POSADA-RAMIREZ M.BELLO-JIMENEZ B.IBARRA-ESCAMILLA A.A.CASTILLO-GUZMAN E.A.KUZIN Instituto Nacional de Astrofísica óptica y Electrónica (INAOE)L. E. Erro 1 Sta. Ma. Tonantzintla Pue. 72824 Mexico CONACyT-Instituto Nacional de Astrofísica óptica y ElectrónicaA. P. 51 y 216 Pue. 72000 Mexico CONACyT-Universidad Autónoma de Nuevo León Av.Universidad S/NSan Nicolas de los Garza N. L. 66451 Mexico Instituto de Investigación en Comunicación óptica Universidad Autónoma de San Luis Potosí Av. Karakorum 1470 S. L. P. 78210 Mexico Universidad Autónoma de Nuevo León Av.Universidad S/NSan Nicolas de los Garza N. L. 66451 Mexico

We report the experimental investigation of an all-fiber multi-wavelength passively Q-switched Er/Yb laser with simultaneous gain-switched pulsed operation by using a thulium-doped fiber as a saturable *** emission is obtained in three wavelength regions with central peaks at around 1546 nm, 1561 nm, and1862 nm. Multi-wavelength emission with separation of approximately 1 nm is obtained around the wavelength regions of 1546 nm and 1561 nm. Stable laser pulses are generated in the pump power range from 3.6 W to7.3 W.

关键词： Fiber laser multi-wavelength