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Effect of Dietary Administration of Lathyrus sativus Pulse on Intestinal Biochemical Parameters in Normal and Scorbutic Guinea Pigs

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Biomedical and Environmental Sciences 2002年第4期15卷 315-322页

作者： ARCHANAAMBA manojkumar 等 FoodToxicologyLaboratory BiomembraneToxicologyLaboratoryIndustialToxicologyReserachCenter M.G.MargP.B.No.80.Lucknw－226001India

Objective In order to investigate that ascorbic acid deficiency is responcible for lathyrus toxicity, the effect of dietary feeding of lathyrus pulse in normal and scorbutic guinea pigs for 3 months, on intestinal biochemical parameters was undertaken. Methods The intestinal brush border membrane (BBM) marker and xenobiotic metabolising enzymes (XME) were assayed. Results Exposure to 80% lathyrus alone and in scorbutic conditions showed significant inhibition of alkaline phosphatase (28%-30%), sucrase (19%) and γ-glutamyl transpeptidase (GGT) (15%-27%) enzymes, while Ca2+-Mg2+-ATPase was significantly inhibited (38%) in scorbutic plus lathyrus treated group. The phase I XME (AHH) remained unchanged while the phase II enzyme glutathione-S-tranferase (GST) was significantly decreased (20%-22%) in lathyrus and scorbutic plus lathyrus treated groups. Quinone reductase (QR) activity was found to be significantly decreased in lathyrus exposed group (20%). The intestinal biomarker contents including hexose (25%-34%) and phospholipids (20%-40%) were significantly reduced in lathyrus and scorbutic plus lathyrus exposed animals, while sialic acid showed a significant decrease (28%) in scorbutic plus lathyrus treated group. However, cholesterol levels were signifcantly enhanced (15%-28%) in lathyrus and scorbutic plus lathyrus treated animals. Conclusion The results indicate that oral feeding of lathyrus pulse to guinea pigs can alter BBM parameters as well as XME, which may result in the intestinal toxicity. Further, ascorbic acid deficiency could be one of the pre-disposing factors of lathyrus toxicity.

关键词： Ascorbic acid BBM Lathyrus sativus Small intestine XME

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Correlation of Levels of Oncostatin M Cytokine in Crevicular Fluid and Serum in Periodontal Disease

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International Journal of Oral Science 2010年第4期2卷 198-207页

作者： manojkumar Thorat Pradeep AR Garima Garg Department of Periodontics Government Dental College and Research Institute

Aim The aim of this study was to measure the level of Oncostatin M （OSM） a gp130 cytokine in the gingival crevicular fluid （GCF） and serum of chronic periodontitis patients and to find any correlation between them before and after periodontal therapy （scaling and root planing,SRP）. Methodology 60 subjects （age 25-50 years） were enrolled into three groups （n=20 per group）,group Ⅰ （healthy）,group Ⅱ （gingivitis） and group III （chronic periodontitis）. Group Ⅲ subjects were followed for 6-8 weeks after the initial periodontal therapy （SRP） as the group Ⅳ （after periodontal therapy）. Clinical parameters were assessed as gingival index （GI）,probing depth （PD）,clinical attachment level （CAL）,and radiographic evidence of bone loss. GCF and serum levels of OSM were measured by using Enzyme Linked Immunosorbent Assay （ELISA）. Results It was found that mean OSM levels had been elevated in both the GCF and serum of chronic periodontitis subjects （726.65 ± 283.56 and 65.59 ± 12.37 pg·mL-1,res-pectively） and these levels were decreased proportionally after the periodontal therapy （95.50 ± 38.85 and 39.98 ± 16.69 pg·mL-1 respectively）. However,OSM was detected in GCF of healthy subjects （66.15 ± 28.10 pg·mL-1） and gingivitis-suffering subjects （128.33 ± 22.96 pg·mL-1） and was found as below the detectable limit （≈0.0 pg·mL-1） in the serum of same subjects. Significant correlation has been found between clinical parameters and GCF-serum levels of OSM. Conclusion Increased OSM level both in the GCF and serum,and the decreased levels after initial periodontal therapy （SRP） may suggest a use as an inflammatory bio-marker in the periodontal disease.

关键词： cytokine Oncostatin M chronic periodontitis gingivitis scaling and root planning

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OKO-SVM:Online kernel optimization-based support vector machine for the incremental learning and classification of the sentiments in the train reviews

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International Journal of Modeling, Simulation, and Scientific Computing 2018年第6期9卷 100-126页

作者： Rashmi K.Thakur manojkumar V.Deshpande MPSTME NMIMS UniversityMumbaiIndia Prestige Institute of Engineering Management&Research Indore India

Online incremental learning is one of the emerging research interests among the researchers in the recent *** sentiment classification through the online incremental learning faces many challenges due to the limitations in the memory and the computing resources available for processing the online *** work has introduced an online incremental learning algorithm for classifying the train *** sentiments available in the reviews provided for the public services are necessary for improving the quality of the *** work proposes the online kernel optimizationbased support vector machine(OKO-SVM)classifier for the sentiment classification of the train *** paper is the extension of the previous work kernel optimizationbased support vector machine(KO-SVM).The OKO-SVM classifier uses the proposed fuzzy bound for modifying the weight for each incoming review database for the particular time *** simulation uses the standard train review and the movie review database for the *** the simulation results,it is evident that the proposed model has achieved a better performance with the values of 84.42%,93.86%,and 74.56%regarding the accuracy,sensitivity,and specificity while classifying the train review database.

关键词： Online incremental learning train reviews sentiment classification kernel optimization train review database.

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Genetic characterization of Stargardt clinical phenotype in South Indian patients using sanger and targeted sequencing

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Eye and Vision 2020年第1期7卷 26-35页

作者： Rajendran Kadarkarai Raj Pankaja Dhoble Rupa Anjanamurthy Prakash Chermakani manojkumar Kumaran Bharanidharan Devarajan Periasamy Sundaresan Department of Genetics Aravind Medical Research Foundation-MaduraiNo.1 Anna NagarMaduraiTamil Nadu 625020India Retina Consultant Department of Vitreo Retinal servicesAravind Eye Hospital-PondicherryPuducherryIndia Department of Paediatrics and Adult strabismus Aravind Eye Hospital-MaduraiMaduraiTamil NaduIndia Department of Bioinformatics Aravind Medical Research Foundation-MaduraiMaduraiTamil NaduIndia

Background:Stargardt disease 1(STGD1;MIM 248200)is a monogenic form of autosomal recessive genetic disease caused by mutation in *** gene has a major role in hydrolyzing N-retinylidene-phosphatidylethanolamine to all-trans-retinal and *** purpose of this study is to identify the frequency of putative disease-causing mutations associated with Stargardt disease in a South Indian ***:A total of 28 clinically diagnosed Stargardt-like phenotype patients were recruited from south *** examination of all patients was carefully carried out by a retina specialist based on the stages of fundus imaging and ERG *** analysis of ABCA4 was performed for all patients using Sanger sequencing and clinical exome ***:This study identified disease-causing mutations in ABCA4 in 75%(21/28)of patients,7%(2/28)exhibited benign variants and 18%(5/28)were negative for the disease-causing ***:This is the first study describing the genetic association of ABCA4 disease-causing mutation in South Indian Stargardt 1 patients(STGD1).Our findings highlighted the presence of two novel missense mutations and an(in/del,single base pair deletion&splice variant)in ***,genetic heterogeneity in ABCA4 mutants requires a larger sample size to establish a true correlation with clinical phenotype.

关键词： Stargardt ABCA4 Macular degeneration Yellow white flecks Mutation detection South Indian population

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纯弯作用下圆形钢管混凝土的强度变形性能

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钢结构(中英文) 2009年第9期 86-87页

作者： manojkumar V.Chitawadagi Mattur C.Narasimhan

介绍处于弯曲作用下,采用不同等级混凝土填充的圆形钢管的强度变形性能。分析钢管厚度、混凝土横截面面积、填充混凝土的强度和混凝土对钢管混凝土弯矩曲率关系的约束等效应。将测出的弯曲强度与采用EC4-1994和LRFD-AISC-1999规范预测... 详细信息

介绍处于弯曲作用下,采用不同等级混凝土填充的圆形钢管的强度变形性能。分析钢管厚度、混凝土横截面面积、填充混凝土的强度和混凝土对钢管混凝土弯矩曲率关系的约束等效应。将测出的弯曲强度与采用EC4-1994和LRFD-AISC-1999规范预测的数值进行对比。对长度为1m的99个构件进行试验,填充混凝土强度范围为20,30,40MPa,钢管径厚比为22.3～50.8。基于试验结果,提出一个用于预测钢管混凝土弯矩曲率关系的交互式模型。

关键词：钢管混凝土梁圆形钢管抗弯强度抗弯刚度

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Antiangiogenic effect of somatostatin receptor subtype 2 on pancreatic cancer cell line:Inhibition of vascular endothelial growth factor and matrix metalloproteinase-2 expression in vitro

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World Journal of Gastroenterology 2004年第3期10卷 393-399页

作者： manojkumar Zheng-RenLiu LaxmiThapa QingChang Da-YuWang Ren-YiQin DepartmentofSurgery TongjiHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430030HubeiProvinceChina DepartmentofObstetricsandGynecology TongjiHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430030HubeiProvinceChina

AIM:To investigate the anti-angiogenic effect of somatostatin receptor subtype 2 (SSTR2) gene transfer into pancreatic cancer cell line PC-3, and the mechanisms involved in this ***: The full length human SSTR2 cDNA was introduced into pancreatic cancer cell line PC-3 by lipofectamine-mediated transfection. Positive clones were screened by G418 and stable expression of SSTR2 was detected by immunohistochemistry SABC methods and RT-PCR. Enzyme-linked immunosorbent assay (ELISA) was used to detect vascular endothelial growth factor (VEGF) levels in the cell culture supernatants of SSTR2-expressing cells, vector control and mock control cells. Furthermore, the expressions of VEGF and matrix metalloproteinase-2 (MMP-2) were detected by immunohistochemistry SABC methods and RT-PCR in these ***: VEGF levels in the cell culture supernatants were significantly reduced in the SSTR2-expressing cells (first week,172.63±21.2ng/L and after two months, 198.85±26.44ng/L)compared with the vector control (first week, 790.39±86.52ng/L and after two months, 795.69±72.35ng/L) and mock control (first week, 786.42±90.62ng/L and after two months,805.32±84.36ng/L) (P<0.05).The immunohistochemical assay showed a significant reduction of the integral optical density of VEGF and MMP-2 in the SSTR2-expressing cells (42.25±8.6 and 70.5±6.25, respectively) compared with the vector control (85.75±12.9 and 110.52±13.5, respectively) and mock control (82.6±9.28 and 113.56±9.62,respectively) (P<0.05).Conversely, the average gray value of VEGF and MMP-2 was significantly increased in the SSTR2-expressing cells (121.56±8.43 and 134.46±19.95, respectively) compared with the vector control (55.72±5.6 and 62.26±12.68,respectively) and mock control cells (58.48±6.2 and 65.49±9.16, respectively) (P<0.05). Moreover, the expressions of VEGF mRNA and MMP-2 mRNA were significantly reduced in the SSTR2-expressing cells (0.1384±0.017 and 0.2343±0.070, respectively) compared with the vector c

关键词：胰腺癌基质金属蛋白酶-2 肿瘤抑制血管内皮生长因子生长抑制素受体亚型2 肿瘤血管生成

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MRI Image Processing with Intelligence:Step towards Computer Aided Diagnostic（CAD） in Healthcare Systems

MRI Image Processing with Intelligence:Step towards Computer...

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2012 International Conference on Software and Computer Applications(ICSCA 2012)

作者： manojkumar.S.Kathane Vilas Thakare Dept of Computer Science S.G.A.UAmravati（M.S）

Diagnostic imaging is a critical tool in healthcare *** are various modalities such as Magnetic resonance imaging（MRI）,computed tomography（CT）,digital mammography,and others,to provide an insight of subject’s body,noninvasively in order to facilitate stakeholders to take decision in ***,in medical research,these technologies has been playing centre role in most of the health care studies and *** a critical component in imaging systems,MRI system has been active area for researchers in computational intelligence and image *** to advances in computing hardware and its easy availability,the performance of MRI system has been improved dramatically since its inception and is able to provide fast imaging,better resolution,immunity to artefacts and cheaper *** of the most important problems in image processing and analysis is segmentation and same is true for biomedical *** approaches has been reported in literature for segmentation of brain,where main objective is separating the pixels associated with different types of tissues like white matter（WM）,gray matter（GM） and cerebrospinal fluid（CSF）.In this paper,we review the methodologies of MRI segmentation that can be used in various diagnostics such as fMRI（Functional MRI）,CMRI（cardiac MRI） and MRA（Magnetic Resonance Angiography）.We will also present the different techniques of computational intelligence to be efficiently used in MRI segmentation.

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Reversal of the phenotype by K-ras^(val12) silencing mediated by adenovirus-delivered siRNA in human pancreatic cancer cell line Panc-1

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World Journal of Gastroenterology 2005年第6期11卷 831-838页

作者： Li-MoChen Huang-YingLe Ren-YiQin manojkumar Zhi-YongDu Rui-JuanXia JingDeng DepartmentofSurgery TongjiHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430030HubeiProvinceChina CollegeofLifeSciences WuhanUniversityWuhan430072HubeiProvinceChina CenterforBiotechnology CollegeofLifeSciencesFujianAgricultureandForestryUniversityFuzhou350002FujianProvinceChina

AIM: To investigate the in vitro antitumor effect of adenovirus-mediated small interfering RNAs (siRNAs) on pancreatic cancer and the associated ***: A 63-nucleotide (nt) oligonucleotide encoding K-ras^val12 and specific siRNA were introduced into pSilencer 3.1-H1, then the H1-RNA promoter and siRNA coding insert were subcloned into pAdTrack to get plasmid pAdTrackH1-K-ras^val12. After homologous recombination in bacteria and transfections of such plasmids into a mammalian packaging cell line 293, siRNA expressing adenovirus AdH1-K-ras^val12 was obtained. Stable suppression of K-ras^val2 was detected by Northern blot and Western blot. Apoptosis in Panc-1 cells was detected by flow ***: We obtained adenovirus AdH1-K-ras^val12 carrying the pSilencer 3.1-H1 cassette, which could mediate gene silencing. Through siRNA targeted K-ras^val12, the oncogenic phenotype of cancer cells was reversed. Flow cytometry showed that apoptotic index of Panc-1 cells was significantly higher in the AdH1-K-ras^val12-treatment group (18.70% at 72 h post-infection, 49.55% at 96 h post-infection)compared to the control groups (3.47%, 3.98% at 72 and 96 h post-infection of AdHl-empty, respectively, 4.21%,3.78% at 72 and 96 h post-infection of AdH1-p53,respectively) (P<0.05).CONCLUSION: These results demonstrate that adenoviral vectors can be used to mediate RNA interference (RNAi)to induce persistent loss of functional phenotypes. In gene therapy, the selective down-regulation of only the mutant version of a gene allows for highly specific effects on tumor cells, while leaving the normal cells untouched. In addition, the apoptosis of pancreatic cancer cell line Panc-1 can be induced after AdH1-K-ras^val12 infection. This kind of adenovirus based on RNAi might be a promising vector for cancer therapy.

关键词：逆转作用显型 K-ras^va/12 噪声抑制腺病毒 siRNA Panc-1 胰腺癌细胞肿瘤

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Feasibility and oncological outcomes of laparoscopic rectal resection following neo-adjuvant chemo-radiotherapy: A systematic review

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World Journal of Surgical Procedures 2015年第1期5卷 147-154页

作者： Pawan Kumar Dhruva Rao manojkumar S Nair Puthucode N Haray Department of Colorectal Surgery Prince Charles Hospital University of South Wales

AIM: To study the feasibility and oncological outcomesfollowing laparoscopic total mesorectal excision(LTME) in patients who have received Neo-adjuvant long course chemo-radiotherapy(LCRT). METHODS: A protocol driven systematic review of published literature was undertaken to assess the feasibility and oncological outcomes following LTME in patients receiving LCRT. The feasibility was assessed using peri-operative outcomes and short term results. The oncological outcomes were assessed using local recurrence, disease free survival and overall ***: Only 8 studies-1 randomized controlled trial, 4 Case Matched/Controlled Studies and 3 Case Series were identified matching the search criteria. The conversion rate was low(1.2% to 28.1%), anastomotic leak rates were similar to open total mesorectal excision(0%-4.1% vs 0%-8.3%). Only 3 studies reported on local recurrence rates(5.2%-7.6%) at median 34 mo follow-up. A single study described disease free survival and overall survival at 3 years as 78.8% and 92.1% respectively. CONCLUSION: LTME following LCRT is feasible in experienced hands, with acceptable short term surgical outcomes and with the usual benefits associated with minimally invasive procedures. The long term oncological outcomes of LTME after LCRT appear to be comparable to open procedures but need further investigation.

关键词： Laparoscopic total mesorectal excision Rectal adenocarcinoma Feasibility Outcomes Neoadjuvant chemo-radiotherapy

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Pros and Cons in Therapeutic Evaluation of Paraoxonase 1 in Nerve Agent Toxicity

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Journal of Life Sciences 2013年第9期7卷 935-940页

作者： manojkumar Valiyaveettil Yonas Alamneh Bhupendra P. Doctor Alfred M. Sciuto Madhusoodana P.Nambiar Blast-Induced Neurotrauma Branch Center for Military Psychiatry and Neuroscience Walter Reed Army Institute of ResearchSilver Spring MD 20910 USA Analytical Toxicology Division US Army Medical Research Institute of Chemical Defense Aberdeen Proving Ground MD21010 USA

PON1 (Paraoxonase 1) has been proposed as an efficient catalytic bioscavenger to combat against OP (organophosphate) and CWNA (chemical warfare nerve agent) toxicity. Unlike stoichiometric bioscavengers such as butyrylcholinesterase, catalytic bioscavengers are cost effective with the advantage of eliminating all the OPs/CWNAs at low doses. Analysis of catalytic bioscavenger efficacy of PON1 showed promising results by various group of researchers. Still, there are large numbers of grey areas which are not addressed so far. One of the major areas of interest is the pharmacokinetic analysis of infused PON1 in multiple animal models. It is shown that previous studies in mice significantly increased half-life of PON1, while recent studies in guinea pigs from our group showed reduced half-life of PON1. Similar results were reported by other research groups in guinea pigs and non-human primates. The short half-life of exogenously administered PON1 in multiple animal models may be due to poor association of PON1 with its endogenous carrier, high density lipoprotein or lower doses of PON1 or a reflection of species difference. These observations warrant the significance of thorough pharmacokinetic analysis of infused PON1 and the development of alternative approaches for successful utility of PON1 as an efficient medical countermeasure against OP/CWNA toxicity.

关键词：对氧磷酶神经性毒剂疗效评价毒性动力学分析丁酰胆碱酯酶高密度脂蛋白动物模型

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