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An Improved Methodology to Overcome Key Issues in Human Fecal Metagenomic DNA Extraction

Genomics, Proteomics & Bioinformatics 2016年第6期14卷 371-378页

作者： Jitendra kumar manoj kumar Shashank Gupta Vasim Ahmed Manu Bhambi Rajesh Pandey Nat Singh Chauhan Department of Biochemistry Maharshi Dayanand UniversityRohtak 124001India Ayurgenomics Unit Translational Research and Innovative Science Through AyurgeomicsCouncil of Scientific and Industrial ResearchInstitute of Genomics and Integrative BiologyNew Delhi 110020India

Microbes are ubiquitously distributed in nature, and recent culture-independent studies have highlighted the significance of gut microbiota in human health and disease. Fecal DNA is the primary source for the majority of human gut microbiome studies. However, further improvement is needed to obtain fecal metagenomic DNA with sufficient amount and good quality but low host genomic DNA contamination. In the current study, we demonstrate a quick, robust, unbiased, and cost-effective method for the isolation of high molecular weight （〉 23 kb） metagenomic DNA （260/280 ratio 〉 1.8） with a good yield （55.8 ± 3.8 ng/mg of feces）. We also confirm that there is very low human genomic DNA contamination （eubacterial： human genomic DNA marker genes = 227.9：1） in the human feces. The newly-developed method robustly performs for fresh as well as stored fecal samples as demonstrated by 16S rRNA gene sequencing using 454 FLX＋. Moreover, 16S rRNA gene analysis indicated that compared to other DNA extraction methods tested, the fecal metagenomic DNA isolated with current methodology retains species richness and does not show microbial diversity biases, which is further confirmed by qPCR with a known quantity of spike-in genomes. Overall, our data highlight a protocol with a balance between quality, amount, user-friendliness, and cost effectiveness for its suitability toward usage for cultureindependent analysis of the human gut microbiome, which provides a robust solution to overcome key issues associated with fecal metagenomic DNA isolation in human gut microbiome studies.

关键词： Metagenomic DNAextraction Gut microbiome Human feces 16S rRNA

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An interval type-2 fuzzy logic controller for TCSC to improve the damping of power system oscillations

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Frontiers in Energy 2013年第3期7卷 307-316页

作者： manoj kumar PANDA Gopinath PILLAI Vijay kumarx Electronics & Computer Engineering Department Indian Institute ofTechnology Roorkee 247667 India Electrical Engineering Department Indian Institute of TechnologyRoorkee 247667 India

In this paper an interval type-2 fuzzy logic controller （IT2FLC） was proposed for thyristor controlled series capacitor （TCSC） to improve power system damping. For controller design, memberships of system variables were represented using interval type-2 fuzzy sets. The three-dimensional membership function of type-2 fuzzy sets provided additional degree of freedom that made it possible to directly model and handle uncertainties. Simulations conducted on a single machine infinite bus （SMIB） power system showed that the proposed controller was more effective than particle swarm optimization （PSO） tuned and type-1 fuzzy logic （T1FL） based damping controllers. Robust performance of the proposed controller was also validated at different operating conditions, various disturbances and parameter variation of the transmission line parameters.

关键词： power system oscillations thyristor controlled series capacitor (TCSC) type-2 fuzzy logic system interval type-2 fuzzy logic controller (IT2FLC)

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Experimental study on spraying performance of biological pesticides in aerial rotary cage nozzle

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International Journal of Agricultural and Biological Engineering 2020年第6期13卷 1-6页

作者： Yu Ru Yangyang Liu Rongjia Qu manoj kumar Patel Collage of Mechanical and Electrical Engineering Nanjing Forestry UniversityNanjing 210037China CSIR-Central Scientific Instruments Organisation Chandigarh 160030India Academy of Scientific and Innovative Research AcSIR-CSIOChandigarh 160030India

Aviation spraying is one of the most important methods for the treatment of forestry pests and diseases,which has a high operation efficiency and *** combination of aviation spray and biological pesticides to reduce the impact of pesticide spray on environment has become an important development in the direction of agricultural *** order to explore the effects of aviation spray parameters on particle size distribution and activity retention of biological pesticide droplets,a system of droplets size test and an activity retention rate test system of biological pesticides were *** related experiments were conducted by changing the structural parameters of the rotary cage nozzle(the diameter of nozzle)and the technical parameters of the operation(rotary speed and flow rate).The results showed that the average relative width S of the droplets of the rotary cage nozzle was close to 1,and the droplet distribution was *** influence factors on the particle size distribution of biological pesticide droplets were rotary speed,nozzle diameter,medium and flow rate in descending *** rotary speed,nozzle diameter,and medium had a significant influence on droplet size *** average activity retention rate of Bacillus thuringiensis was 88.83%,and the germination rate of Beauveria bassiana was 89.80%.The rotary speed had a significant effect on the activity retention rate of bacterial and fungal biological pesticides,and it was negatively *** rotary speed was between 3000-5000 r/min,and the activity retention rate was *** research results can provide guidance for spraying biological pesticides in aerial plant protection operations with rotary cage nozzle.

关键词： forestry disease and pest control biological pesticide activity retention rate aerial rotary cage nozzle droplet distribution

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Real-time Privacy Preserving Framework for Covid-19 Contact Tracing

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Computers, Materials & Continua 2022年第1期70卷 1017-1032页

作者： Akashdeep Bhardwaj Ahmed A.Mohamed manoj kumar Mohammed Alshehri Ahed Abugabah School of Computer Science University of Petroleum&Energy Studies(UPES)Dehradun248007India Department of Computer Science College of Computer and Information SciencesMajmaah UniversityMajmaah11952Saudi Arabia Department of Information Technology Faculty of Computer and InformationAssiut UniversityAssiut71515Egypt Department of Information Technology College of Computer and Information SciencesMajmaah UniversityMajmaah11952Saudi Arabia Department of Information System College of Technological InnovationZayed UniversityAbu DhabiUnited Arab Emirates

The recent unprecedented threat from COVID-19 and past epidemics,such as SARS,AIDS,and Ebola,has affected millions of people in multiple *** have shut their borders,and their nationals have been advised to *** variety of responses to the pandemic has given rise to data privacy *** prevention and control strategies as well as disease control measures,especially real-time contact tracing for COVID-19,require the identification of people exposed to *** tracing frameworks use mobile apps and geolocations to trace ***,while the motive may be well intended,the limitations and security issues associated with using such a technology are a serious cause of *** are growing concerns regarding the privacy of an individual’s location and personal identifiable information(PII)being shared with governments and/or health *** study presents a real-time,trust-based contact-tracing framework that operateswithout the use of an individual’sPII,location sensing,or gathering GPS *** focus of the proposed contact tracing framework is to ensure real-time privacy using the Bluetooth range of individuals to determine others within the *** research validates the trust-based framework using Bluetooth as practical and *** our proposed methodology,personal information,health logs,and location data will be secure and not *** research analyzes 100,000 tracing dataset records from 150 mobile devices to identify infected users and active users.

关键词： Privacy contact tracing mobile apps Bluetooth Covid epidemic

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Pulmonary infarct masquerading as community-acquired pneumonia in the COVID-19 scenario: A case report

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World Journal of Respirology 2024年第1期13卷 1-6页

作者： K K Mujeeb Rahman Gopal Durgeshwar Prasanta R Mohapatra manoj kumar Panigrahi Siladitya Mahanty Pulmonary Critical Care and Sleep Medicine All India Institute of Medical SciencesBhubaneswarBhubaneswar 751019India Department of Radiodiagnosis All India Institute of Medical SciencesBhubaneswar 751019India

BACKGROUND Pulmonary embolism(PE)requires a high degree of clinical suspicion for its diagnosis and can mimic pneumonia due to its clinical,radiological,and laboratory ***-existence of PE and pneumonia can also occur,which is surprisingly more common than *** SUMMARY Here,we report a case of a young male who initially presented during the peak of the coronavirus disease 2019 pandemic with features of *** was kept under observation and was later diagnosed and treated for a right main pulmonary artery embolism without any identifiable source of *** PE and pneumonia share common clinical,radiological,and laboratory findings that may delay the diagnosis of *** disproportionate to the extent of radiological involvement could be an indicator of an underlying PE.

关键词： Pneumonia Pulmonary embolism Deep vein thrombosis Pulmonary infarct Case report

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Signal Conducting System with Effective Optimization Using Deep Learning for Schizophrenia Classification

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Computer Systems Science & Engineering 2023年第5期45卷 1869-1886页

作者： V.Divya S.Sendil kumar V.Gokula Krishnan manoj kumar School of Electrical and Electronics Engineering Sathyabama Institute of Science and TechnologyChennaiTamilnaduIndia Department of Electrical and Electronics Engineering S A Engineering CollegeChennaiTamilnaduIndia Department of Computer Science and Information Technology CVR College of EngineeringMangalpallyHyderabadTelanganaIndia School of Computer Science University of Petroleum and Energy StudiesDehradunIndia

Signal processing based research was adopted with Electroencephalogram(EEG)for predicting the abnormality and cerebral *** proposed research work is intended to provide an automatic diagnostic system to determine the EEG signal in order to classify the brain function which shows whether a person is affected with schizophrenia or *** detection and intervention are vital for better ***,the diagnosis of schizophrenia still depends on clinical observation to *** reliable biomarkers,schizophrenia is difficult to detect in its early phase and hence we have proposed this *** this work,the EEG signal series are divided into non-linear feature mining,classification and validation,and t-test integrated feature selection *** this work,19-channel EEG signals are utilized from schizophrenia class and normal ***,the datasets initially execute the splitting process based on raw 19-channel EEG into 6250 sample point’s *** this process,1142 features of normal and schizophrenia class patterns can be *** other hand,157 features from each EEG patterns are utilized based on Non-linear feature extraction process where 14 principal features can be identified in terms of considering the essential *** last,the Deep Learning(DL)technique incorporated with an effective optimization technique is adopted for classification process called a Deep Convolutional Neural Network(DCNN)with mayfly optimization *** proposed technique is implemented into the platform of MATLAB in order to obtain better results and is analyzed based on the performance analysis framework such as accuracy,Signal to Noise Ratio(SNR),Mean Square Error,Normalized Mean Square Error(NMSE)and *** comparison,the proposed technique is proved to a better technique than other existing techniques.

关键词： Deep learning optimization algorithm signal conducting system schizophrenia convolutional neural network mayfly optimization algorithm

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EUS-guided Gall Bladder Drainage in Severe Liver Disease: A Single-center Experience in Critically Ill Cirrhotics

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Journal of Clinical and Translational Hepatology 2018年第1期6卷 35-39页

作者： Kapil Dev Jamwal manoj kumar Sharma Rakhi Maiwall Barjesh kumar Sharma Shiv kumar Sarin Department of Hepatology Institute of Liver and Biliary SciencesD1Vasant KunjNew DelhiIndia Department of Hepatology Institute of Liver and Biliary SciencesD1Vasant KunjNew Delhi 110070India

Background and Aims:Acute calculous cholecystitis with impending gall bladder perforation in severe liver diseases including decompensated cirrhosis and acute-on-chronic liver failure(ACLF)is difficult to manage,due to the procedures such as cholecystectomy and percutaneous cholecystostomy being associated with high risk and complications in these ***:Four cases of severe liver disease with acute calculous cholecystitis who presented to the Institute of Liver and Biliary Sciences(New Delhi,India)for further management were included in the study if they underwent endoscopic ultrasound-guided gall bladder drainage(EUS-GBD).The patients were followed up for a minimum of 3 months and outcomes were ***:Four cases of severe liver disease(three ACLF and one decompensated cirrhosis),with model for end-stage liver disease scores of 24,26,23 and 25 respectively,presented with acute calculous cholecystitis(Tokyo grade III)and systemic sepsis(high total leukocyte counts).Their international normalized ratios were 2.3,2.6,2.2 and 2.9 respectively,and two were in shock,requiring inotropes at *** of the abdomen confirmed hugely distended gall bladder with stone impacted at the neck and moderate *** these cases underwent EUS-GBD by linear echo endoscope,and had the gastric wall punctured in the antrum using a 19G access needle followed by dilatation of the tract using controlled radial expansion balloon and Sohendra *** three cases,the plastic stents were *** the fourth case,a Nagi stent was *** the patients recovered and were discharged within a ***:EUS-GBD is challenging in severe liver disease but represents a life-saving procedure,and hence can be attempted in such critically ill patients with utmost care and precaution.

关键词： ACLF Acute-on-chronic liver failure Cholecystitis Ascites

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MicroRNA-99a mimics inhibit M1 macrophage phenotype and adipose tissue inflammation by targeting TNFα

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Cellular & Molecular Immunology 2019年第5期16卷 495-507页

作者： Anant Jaiswal Sukka Santosh Reddy Mohita Maurya Preeti Maurya manoj kumar Barthwal Division of Pharmacology Council of Scientific and Industrial Research-Central Drug Research Institute(CSIR-CDRI)Lucknow 226031 U.P.India Academy of Scientific and Innovative Research New Delhi 110025India

In human adipose tissue and obesity,miR-99a expression is negatively correlated with ***,the present study investigated the role of miR-99a in macrophage phenotype activation and adipose tissue inflammation.M2 BMDMs showed a significant increase in miR-99a expression when compared to the M0 and M1 ***-switching experiments established an association between upregulated miR-99a expression and the M2 *** of miR-99a prevented M1 phenotype activation and attenuated bactericidal ***,knockdown of miR-99a abolished M2 phenotype *** means of in silico target prediction tools and a luciferase reporter assay,TNFαwas identified as a direct target of *** of TNFαrecapitulated the effect of miR-99a overexpression in M1 *** a db/db mice model,miR-99a expression was reduced in eWAT and F4/80+*** overexpression of miR-99a in db/db mice attenuated adipocyte hypertrophy with increased CD301 and reduced CD86 *** cytometry analysis also showed an increased M2 and a reduced M1 macrophage *** of miR-99a also improved the diabetic dyslipidemia and insulin signaling in eWAT and liver,with an attenuated expression of gluconeogenesis and cholesterol metabolism genes in the ***,adoptive transfer of miR-99a-overexpressing macrophages in the db/db mice recapitulated in vivo miR-99a mimic effects with increased M2 and reduced M1 macrophage populations and improved systemic glucose,insulin sensitivity,and insulin signaling in the eWAT and *** present study demonstrates that miR-99a mimics can regulate macrophage M1 phenotype activation by targeting TNFα.miR-99a therapeutics in diabetic mice reduces the adipose tissue inflammation and improves insulin sensitivity.

关键词： inflammation macrophage CD86

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Entropically favoured through space charge transfer‘lighted’photosensitizing assemblies for‘metal free’regulated photooxidation of alcohols and aldehydes

Aggregate

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Aggregate 2022年第2期3卷 143-153页

作者： Gurpreet Kaur Harpreet Kaur Mandeep Kaur manoj kumar Vandana Bhalla Department of Chemistry Centre of Advance Studies-IIGuru Nanak Dev UniversityAmritsarIndia

Strong acceptor-weak acceptor system FN-TPy has been designed and synthesized,which undergoes solvent dependent self-assembly in mixed aqueous media to generate through space intermolecular charge transfer *** as-prepared entropically favoured assemblies of FN-TPy exhibit excellent photostability and photosensitizing properties in the assembled state to activate aerial oxygen for efficient generation of reactive oxygen species through Type-I and Type-II *** FN-TPy exhibits excellent potential for regulated oxidation of alcohols and aldehydes under mild reaction conditions(visible light irradiation,aqueous media,room temperature)using aerial oxygen as the‘oxidant’.The present study demonstrates the potential of FN-TPy assemblies to catalyse controlled oxidation of benzyl alcohol to benzoic acid.

关键词： aggregated assemblies molecular oxygen activation oxidations photosensitizer through space charge transfer

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Preventable iatrogenic cause of foot-drop in knee injuries with literature review

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Chinese Journal of Traumatology 2022年第4期25卷 224-231页

作者： Anant Krishna Sumit Arora Rakesh Goyal Manish kumar Nirup Naik manoj kumar Department of Orthopaedics All India Institute of Medical SciencesAnsari NagarNew Delhi110029India Department of Orthopaedic Surgery Maulana Azad Medical College&Associated Lok Nayak HospitalNew Delhi110002India

Purpose Common peroneal nerve palsy is quite disabling and every effort should be made to prevent its injury during the *** We retrospectively reviewed the prospectively collected data of 7 cases of tibial plateau fractures in association with proximal fibula fracture from January 2019 to September 2019 who presented to emergency room of our *** In addition to fibular neck fracture,the first case had type 6 tibial plateau displaced fracture and the second case had displaced acetabular fracture with instability of knee with tibial tuberosity *** peroneal nerve palsy developed following application of distal tibial skeletal traction in both the *** 6 such cases remained neurologically intact as traction was not applied to *** Such iatrogenic complication could have been prevented if the injury pattern of"concomitant medial and lateral columns"of the proximal leg is kept in mind by the treating surgeon before applying skeletal traction.

关键词： Common peroneal neuropathies Tibial plateau fractures Proximal fibula fractures Traction palsy

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