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Bioremediation in Mangrove Sediments Impacted by Oil Using Two Types of Fertilizers NPK and OSMOCOTE, Brazil

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Agave sisalana extract induces cell death in Aedes aegypti hemocytes increasing nitric oxide production

Open Journal of Marine Science（海洋科学期刊） 2012年第4期

作者： Danusia Ferreira Lima Olívia Maria Cordeiro de Oliveira manoel jeronimo moreira cruz Jorge Alberto Triguis Antonio Fernando de S. Queiroz

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Asian Pacific Journal of Tropical Biomedicine 2016年第5期6卷 396-399页

作者： Louise Helena Guimaraes de Oliveira Patricia Alexandria Paiva Silva de Sousa Fabrine Felipe Hilario Gabriel Joventino Nascimento Joao Paulo Saraiva Morais Everaldo Paulo de Medeiros manoel Francisco de Sousa Fabiola da cruz Nunes Biotechnology Center Federal University of Paraiba Embrapa Algodao Brazilian Agricultural Research Corporation

Objective: To investigate the effects of Agave sisalana(A. sisalana) extract on Aedes aegypti(Ae. aegypti) primary cell ***: Cells of Ae. aegypti were exposed to different concentrations of A. sisalana crude extract(0.18–6.00 mg/m L) for 24 h. Then, the cells were labeled with propidium iodide and subjected to fluorescence microscopy to verify cell viability. In addition, nitric oxide production was ***: Results showed that cells exposed to 6 mg/m L of the crude extract presented a greater percentage of death when compared to control(73.8% ± 9.6% vs. 34.6% ± 9.6%).Furthermore, there was an increase in the nitric oxide production in cells exposed to 6 mg/m L of A. sisalana crude extract [(0.81 ± 0.08) mmol/L] compared to control group[(0.41 ± 0.18) mmol/L].Conclusions: The results show that A. sisalana is cytotoxic to Ae. aegypti and may be used as raw material for new eco-friendly and inexpensive insecticides, since sisal industry discards the liquid waste for the extraction of plant fiber.

关键词： Sisal Cell culture Mosquito Insecticide

The Difference of the Impact of Elective Cholecystectomy Surgery in the Immune Response

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Surgical Science 2018年第8期9卷 243-247页

作者： Rosamaria Rodrigues Gomes manoel Messias Lima Neto Ayrton Silva Alves Cristiane Monteiro da cruz Medical School Centro Universitário Cesmac Maceió Brazil

Introduction: The inflammatory response is essential to initiate the healing process, and in this response there is an increase in white blood cells and pro-inflammatory compounds. Creactive proteins (CRP), together with the blood leukocyte count, have been used to study the immune response. Due to the risk of infection and exacerbated inflammatory response of the patients undergoing surgical procedure, it is relevant to investigate the pre and postoperative inflammatory response of those individuals. Objective: To determine the difference in the impact of elective cholecystectomy surgery on the patient’s immune response. Methods: The present study was cross-sectional, where a group included patients who underwent elective cholecystectomy procedure. Patients who were pregnant, under 18 years, of indigenous communities or with other conditions that depress the immune system, as well as those on immunosuppressive medications, were excluded. Results: CRP and leukocyte counts indicated a mean increase of 5.97-fold (95% CI 3.76 to 8.34, SD 6.98) and 1.97-fold (95% CI 1.71 to 2.24, SD 1.97) respectively, both being evaluated before and after surgery. Discussion: In the present study, to avoid a possible methodological bias, it was chosen to evaluate the patients submitted to videolaparoscopic cholecystectomy only and the hypothesis was confirmed that there is an increase in CRP and leukocyte count as a response to surgical trauma. Conclusion: There was an elevation of the inflammatory markers in patients submitted to surgical trauma when comparing the exams collected in the preoperative period and after the surgical aggression.

关键词： Cholecystectomy Inflammation Leukocytes C-Reactive Protein

Prevalence of sarcopenia using different methods in patients with non-alcoholic fatty liver disease

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World Journal of Hepatology 2022年第8期14卷 1643-1651页

作者： Naiade Silveira Almeida Raquel Rocha Claudineia Almeida de Souza Ana Carolina Sirelli da cruz Bruna dos Reis Ribeiro Luiza Valois Vieira Carla Daltro Rafael Silva manoel Sarno Helma Pinchemel Cotrim Programa de Pós-Graduação em Medicina e Saúde Universidade Federal da BahiaSalvador 40110-060BABrazil Departamento de Ciência da Nutrição Escola de NutriçãoUniversidade Federal da BahiaSalvador 40110-060Brazil Caliper Clínica e Escola de Imagem Salvador 41810-012Brazil

BACKGROUND Sarcopenia is a clinical condition associated with several liver diseases and it includes non-alcoholic fatty liver disease(NAFLD)in its broad spectrum as steatosis,steatohepatitis and ***,the criteria to define sarcopenia are diverse,and even those established in consensus have been discussed regarding their performance in making an accurate *** To evaluate the prevalence of sarcopenia,using different methods,in patients with NAFLD,and its association with clinical-anthropometric *** This was an observational study of outpatients with *** was defined by the European Working Group Consensus on Sarcopenia in Older People of 2010(EWGSOP1)and 2018(EWGSOP2).The skeletal muscle index was used to estimate muscle mass,handgrip strength was assessed using the dynamometer and physical performance by walking a distance of four meters at usual walking *** non-invasive fibrosis scores,fibrosis-4(FIB-4)index and Aspartate aminotransferase to platelet ratio index(APRI),were used to assess the absence and presence of *** Fifty-seven individuals with NAFLD were evaluated,the mean age(SD)was 52.7(11.3)years and 75.4%were *** assessed by FIB-4 and APRI was observed in 3.7%and 16.6%of patients with NAFLD,*** diagnosis of sarcopenia was identified only by EWGSOP1 in 3.5%of NAFLD patients,and the prevalence of probable/pre-sarcopenia was higher using the EWGSOP2 consensus at 26.3%,when compared to 1.8%with *** defined by EWGSOP1,was associated with grade I steatosis,but without overweight(P0.05).EWGSOP2 showed a greater number of patients with probable sarcopenia,and who were overweight(12(80.0%)),with a higher degree of steatosis[11(73.3%)and presence of fibrosis(1(6.7%),FIB-4 and 3(20.0%),APRI]compared to EWGSOP1[1(100%),0(0.0%),0(0.0%),FIB-4 and 0(0.0%),APRI,respectively].CONCLUSION The presen

关键词： Non-alcoholic fatty liver disease Sarcopenia Muscle strength Physical performance Liver fibrosis

Mediastinal Liposarcoma: Case Report and Literature Review

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Journal of Cancer Therapy 2021年第4期12卷 208-220页

作者： jeronimo Garcialopez De Llano Maria Fernanda Mijares Vanitha Vasudevan Rodrigo Arrangoiz Amit Sastry Frank De la cruz Jennifer Fernandez Garcia Adrian Legaspi Center for Advanced Surgical Oncology Palmetto General Hospital Hialeah USA

Liposarcomas (LS) are the second most common type of soft tissue malignancies in adults;they arise from mesenchymal cells and account for 1% of all adult cancers and 15% of all pediatric cancers. The site of origin can be from anywhere there is fat in the human body. LS are classified based on the primary site of origin, and mediastinal LS are extremely rare. When mediastinal neoplasms are stratified based on histology, they represent less than 1% of all mediastinal tumors. To date, less than 150 cases have been reported in the English literature. This article aims to present an unusual case of an extremely rare malignancy and perform a systematic review of the latest literature. In this report, our group is documenting the presentation, management, and outcome of a 65-year-old male patient with a massive anterior mediastinal primary LS. The tumor was displacing the mediastinum into the right chest, occupying most of the left chest, and pushing the diaphragm into the abdomen. Mediastinal liposarcomas are extremely rare malignancies and can prove to be challenging to diagnose and treat. Aggressive surgical treatment with R0 resection is the gold standard, however, tumor biology in many cases is associated with variable growth rates and encroachment of adjacent vital structures and blood vessels. When complex anatomical structures preclude an R0 resection, there is a high incidence of local recurrence. In cases where there is a high risk of recurrence, radiotherapy is indicated and chemotherapy has a more limited role.

关键词： Liposarcoma (LS) Well-Differentiated Liposarcoma (WDLS) Dedifferentiated Liposarcoma (DDLS)

Description of the technique of bone marrow harvesting in the coxal tuberosity for isolation and culture of mesenchymal stem cells of buffaloes(Bubalus bubalis)

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Stem Cell Discovery 2014年第1期4卷 8-12页

作者： Carolina Nogueira de Moraes Amanda jeronimo Listoni Leandro Maia Carla Martins de Queiroz Flavia Caroline Destro Eunice Oba Fernanda da cruz Landim-Alvarenga Department of Animal Reproduction and Veterinary Radiology Sao Paulo State UniversityBotucatuBrazil

Several studies with mesenchymal stem cells (MSCs) have been developed in many species because of its ability to differentiate into other mesoderm lineages, capacity of self-regeneration, low immunogenicity, paracrine, anti-inflammatory, immunomodulatory and antiapoptotic effects which make them a promissory source to be used in therapeutic strategies. The aim of this study is to report the technique of harvest of bone marrow (BM) in the coxal tuberosity (CT) of buffaloes and its processing and cultivation. For this, after anesthetic block from the region corresponding to the CT, bone marrow harvesting was performed with a myelogram’s needle. The samples collected showed plastic adherence with 96 hours and took approximately 32 days to reach 80% confluence. And then differentiation into adipogenic and osteogenic lineages was performed. Samples showed morphological changes during differentiation protocol, but not all presented production of extracellular deposits of calcium or intracellular fat droplets. The anatomical site tested showed to be an alternative site of harvest of BM once provided with the appropriate isolation and culture of the mononuclear fraction. Moreover, the procedure was performed without difficulty and with great security. Based on this, it can be concluded that CT is an excellent anatomical site for isolation and culture of MSCs and the proposed technique is viable and feasible to be held in buffaloes.

关键词： Buffaloes MSCs Coxal Tuberosity Culture

Molecular Diagnosis of Thyroid Nodules and Its Future Implications for the Management of Thyroid Cancer

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International Journal of Otolaryngology and Head & Neck Surgery 2021年第5期10卷 398-418页

作者： Rodrigo Arrangoiz jeronimo Garcialopez De Llano Maria Fernanda Mijares Gonzalo Fernandez-Christlieb Vanitha Vasudevan Amit Sastry Adrian Legaspi Jennifer Fernandez Frank de la cruz Fernando Cordera Daniel Margain Center for Advanced Surgical Oncology Steward Health Hialeah FL USA American British Cowdray Medical Center Mexico City Mexico

Molecular testing in thyroid nodules and thyroid cancer is rapidly evolving;care must be used when incorporating molecular testing for thyroid nodules into clinical practice. A clear appreciation of the goals and restraints of molecular testing must be integrated into how physicians use and explain molecular testing to patients. Molecular tests can help rule in cancer for indeterminate thyroid nodules with very specific mutations for thyroid cancer, such as BRAF and RET/PTC, and can help reduce the rates of completion thyroidectomies in this era of de-escalation of the management of thyroid disease. The positive predictive value (PPV) of malignant cytology (Bethesda VI) is 98%;and even though molecular testing improves specificity and PPV, it falls short of this ideal for other mutations. We present a detailed evaluation of the current state of molecular testing and their clinical relevance in the setting of diagnostic utility and their impact on surgical decision-making. By recapitulating the clinical impact of these tests and some of the related drawbacks, we hope to provide adequate up to date information of the appropriate utilization of these tools in the management of indeterminate or suspicious thyroid nodules and highlight future directions on their utilization for the management of thyroid cancer.

关键词： Thyroid Cancer Papillary Thyroid Cancer Papillary Thyroid Microcarcinoma Thyroid Nodule Thyroid Cancer Treatment Molecular Studies for Thyroid Cancer Affirma Thyroseq

Current Understanding of Papillary Thyroid Carcinoma

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International Journal of Otolaryngology and Head & Neck Surgery 2021年第3期10卷 184-221页

作者： Rodrigo Arrangoiz jeronimo Garcialopez De Llano Maria Fernanda Mijares Gonzalo Fernandez-Christlieb Vanitha Vasudevan Amit Sastry Adrian Legaspi Jennifer Fernandez Frank de la cruz Fernando Cordera Daniel Margain Center for Advanced Surgical Oncology Florida USA American British Cowdray Medical Center Mexico City Mexico Sociedad Quirurgica S.C. at the American British Cowdray Medical Center Mexico City

The term thyroid neoplasm incorporates tumors that originate from follicular cells and those that arise from parafollicular cells (C cells). Differentiated thyroid cancer, which originates from follicular cells, includes papillary thyroid carcinoma (PTC), follicular thyroid carcinoma (FTC), oncocytic cell carcinoma (Hürthle), poorly differentiated carcinoma, and anaplastic thyroid carcinoma (ATC). PTC tends to have an indolent clinical course with low morbidity and mortality. However, this entity has a broad range of biological and clinical behavior that can result in disease recurrence and death, depending on patient and tumor characteristics and the initial treatment approach. PTC is the most common form of well-differentiated thyroid cancer (WDTC) and based on the most recent statistics, accounts for approximately 89.4% of all thyroid malignancies. PTC appears as an irregular solid or cystic nodule in normal thyroid parenchyma. PTC has the propensity for lymphatic invasion, but it is less likely to have hematogenous spread. Around 11% of patients with PTC present with distant metastases outside the neck and mediastinum. This manuscript with review the current understanding of the epidemiology, pathology, molecular characteristics, prognostic factors, and dynamic risk stratification of PTC centered on an evidence-based and personalized approach.

关键词： Thyroid Cancer Papillary Thyroid Cancer Papillary Thyroid Microcarcinoma Thyroid Nodule Thyroid Cancer Treatment Molecular Studies for Thyroid Cancer

The Role of Preoperative Ultrasound and Sestamibi Scintigraphy in the Surgical Management of Primary Hyperparathyroidism: A Review of Literature

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International Journal of Otolaryngology and Head & Neck Surgery 2022年第2期11卷 91-105页

作者： Daniel Margain Rodrigo Arrangoiz Adrian Legaspi Vanitha Vasudevan Amit Sastry Frank De La cruz jeronimo Garcialopez De Llano Jennifer Fernandez Loisani Galindo Noah Llaneras Matthew Farrell Gonzalo Fernandez-Christlieb Fernando Cordera Center for Advanced Surgical Oncology Hialeah FL USA American British Cowdray Medical Center Mexico City Mexico Sociedad Quirurgica S.C. at the American British Cowdray Medical Center Mexico City Mexico

Background: Primary hyperparathyroidism (pHPT) is one of the most common endocrine diseases and the most common cause of hypercalcemia. Since routine laboratory testing, the prevalence of the disease has increased from 0.1% to 0.4% worldwide. The only curative treatment is parathyroidectomy. Nowadays, preoperative localization studies have become standard before surgical treatment, and the first stage imaging methods are cervical ultrasonography (US) and/or Sestamibi scintigraphy. Objectives: To describe the accuracy of US and Sestamibi for detection of hyperfunctioning parathyroid adenomas preoperatively in patients with confirmed pHPT from our medical institution. Results: This is a retrospective study from a prospectively kept database that included thirty-one patients with the confirmed diagnosis of pHPT clinically and biochemically. The average age was 57.8 years old. Preoperative US and surgery findings were compared with a sensitivity of 51%. Preoperative Sestamibi and surgery findings were compared resulting in a sensitivity of 71%. Both imaging methods combined, resulted in a sensitivity of 80%. Conclusion: In patients with pHPT, Sestamibi is an effective method for localizing parathyroid pathology preoperatively, but the false negative rate can be high. US tends to have a wider range explained by the operator-dependent factor. The combination of US and Sestamibi reduces the rate of false negatives, as reported in international literature. It is important to mention that these studies cannot be used as a confirmatory test for this disease. It should only be used as an adjunct to help plan the operation.

关键词： Parathyroid Glands Primary Hyperparathyroidism Parathyroid Adenoma Parathyroid Hyperplasia Parathyroidectomy Cervical Ultrasound Sestamibi Scintigraphy Limited Neck Exploration