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Supplementation of L-ascorbic acid improves the in vitro development of buffalo(Bubalus bubalis)embryos and alters the expression of apoptosis-related genes

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Asian pacific Journal of Reproduction 2021年第1期10卷 36-42页

作者： Mayank Roshan Diksha Dua Ankur Sharma Manish Tiwari Manoj Kumar singh Suresh Kumar Singla Prabhat Palta Radhay Sham Manik manmohan singh chauhan Embryo Biotechnology Lab Animal Biotechnology CentreICAR-National Dairy Research InstituteKarnalIndia

Objective:To study the effect of L-ascorbic acid supplementation on the in vitro development of buffalo embryos and evaluate the relative mRNA abundance of some pro-apoptotic,anti-apoptotic,and embryonic development-related ***:In experiment 1,we evaluated the effect of the addition of 0(control),50,and 100μM L-ascorbic acid to the in vitro maturation medium on the developmental competence in terms of blastocyst rate and relative mRNA abundance of some pro-apoptotic(BAX,BID),anti-apoptotic(BCL-XL,MCL1),and embryonic development(GDF9,BMP15)related *** on the results,we chose 50μM as the suitable dose of L-ascorbic acid for the subsequent *** further evaluated the blastocyst rates following the addition of 50μM L-ascorbic acid to the in vitro culture medium(experiment 2),and in vitro maturation and in vitro culture media(experiment 3).In all three experiments,the maturation and culture media devoid of L-ascorbic acid served as the control ***:The blastocyst rate after adding 50μM L-ascorbic acid to the in vitro maturation medium was significantly higher than the control group(P<0.05),whereas 100μM L-ascorbic acid exhibited a negative effect on the blastocyst *** blastocyst rates for embryos cultured in 50μM L-ascorbic acid in the in vitro culture medium alone and both in vitro maturation and in vitro culture media were significantly higher than their corresponding control groups(P<0.05).The relative mRNA abundance of BAX significantly decreased in blastocysts produced after the addition of 50μM L-ascorbic acid as compared with the control group(P<0.05),whereas,for MCL1,it significantly decreased in blastocysts produced after the addition of 100μM L-ascorbic acid(P<0.05).Conclusions:The supplementation of 50μM L-ascorbic acid to in vitro maturation and in vitro culture media supports in vitro embryonic development in buffaloes by improving developmental competence and altering the expression of apoptosis-related genes.

关键词： Buffalo Blastocyst In vitro embryo production L-ascorbic acid Oocyte

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The dynamic effects of solar eclipses of October 25,2022,and October 14,2023,on GPS-derived total electron content

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Geodesy and Geodynamics 2025年第1期16卷 75-86页

作者： Vishal chauhan Vishakha Rakesh singh Shishupal singh Vikram singh Om Prakash singh Department of Physics Graphic Era Hill University Department of Petroleum Engineering Graphic Era (Deemed to be University) Department of Physics St.John's College Department of Applied Sciences & Humanities Institute of Engineering & Technology

This study analyzes total electron content(TEC) variations during two solar eclipse events that occurred on October 25, 2022, and October 14, 2023. The solar eclipse of October 25, 2022, was a partial solar eclipse, while the eclipse of October 14, 2023, was an annular solar eclipse. For this study, the data of eight International GNSS Service(IGS) stations from different eclipse coverage zones are used to analyze TEC variations. It is found that the stations located in the maximum eclipse cover zone exhibited notable decreases in TEC values. The minimum variation of about 11.76% in TEC values is observed at the station situating in about 20 percent eclipse cover zone, while it varies from 22% to 38% at the stations falling in about 60 percent eclipse cover zone. The highest variation in TEC values of about 44% is found at the stations in about 80 percent eclipse cover zone. The time of occurrence of maximum depletion in TEC values at each station is in line with their longitudinal sequence. Atmospheric gravity waves(AGWs) are also observed by performing wavelet analysis on TEC data. The global TEC maps visualize and confirm observed TEC variations, providing spatial and temporal insights into the ionospheric response. This analysis highlights the influence of station location and eclipse coverage on the magnitude and spatial distribution of TEC variations.

关键词： Solar eclipse Ionosphere GPS TEC AGW

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A comprehensive investigation on nonlinear vibration and bending characteristics of bio-inspired helicoidal laminated composite structures

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Applied Mathematics and Mechanics(English Edition) 2025年第1期46卷 81-100页

作者： S.SAURABH R.KIRAN D.singh R.VAISH V.S.chauhan School of Mechanical and Materials Engineering Indian Institute of Technology Mandi

Bio-inspired helicoidal composite laminates, inspired by the intricate helical structures found in nature, present a promising frontier for enhancing the mechanical properties of structural designs. Hence, this study provides a comprehensive investigation into the nonlinear free vibration and nonlinear bending behavior of bio-inspired composite plates. The inverse hyperbolic shear deformation theory(IHSDT) of plates is employed to characterize the displacement field, with the incorporation of Green-Lagrange nonlinearity. The problem is modeled using the C0finite element method(FEM), and an in-house code is developed in the MATLAB environment to solve it numerically. Various helicoidal layup configurations including helicoidal recursive(HR), helicoidal exponential(HE), helicoidal semi-circular(HS), linear helicoidal(LH), and Fibonacci helicoidal(FH)with different layup sequences and quasi-isotropic configurations are studied. The model is validated, and parametric studies are conducted. These studies investigate the effects of layup configurations, side-to-thickness ratio, modulus ratios, boundary conditions, and loading conditions at different load amplitudes on the nonlinear vibration and nonlinear bending behaviors of bio-inspired composite plates. The results show that the laminate sequence exerts a substantial impact on both nonlinear natural frequencies and nonlinear bending behaviors. Moreover, this influence varies across different side-to-thickness ratios and boundary conditions of the bio-inspired composite plate.

关键词： bio-inspired composite plate finite element method (FEM) Green-Lagrange helicoidal inverse hyperbolic shear deformation theory (IHSDT) nonlinear O322

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Assessing tectonic influence on landscape evolution:case study of the Nandakini Watershed,Western Himalaya

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Journal of Mountain Science 2025年第2期22卷 666-680页

作者： Rakesh singh Pranati SAHU Shalini KUMARI Vishal chauhan Department of Petroleum Engineering and Earth Sciences Graphic Era(Deemed to be University)Dehradun 248002India School of Basic and Applied Sciences SGRR UniversityDehradun 248001India Graphic Era Hill University Dehradun 248002India

Understanding the active tectonic processes in the Nandakini Watershed is imperative for evaluating geological hazards and seismic risks,as well as for informing land-use planning and natural resource management strategies in the *** geomorphology serves as a vital tool for characterizing recent tectonic *** research employs GIS techniques to elucidate tectonic activity and its influence on drainage patterns in the Nandakini Watershed,utilizing morphometric parameters derived from SRTM DEM *** indices are employed to assess the tectonic movement within drainage basins,capturing both areal and linear factors such as drainage density,texture,circulatory and bifurcation ratios,and stream length *** linear and areal morphometric indices are categorized into three classes representing varying degrees of active tectonic *** classifications are then utilized to compute the relative active tectonic index(IRAT).In addition,geomorphic parameters include hypsometric integral,stream length-gradient index,normalized steepness index,chi gradient index,and swath *** majority of the studied region is in an extremely high to moderately active tectonic ***-scale faults and thrusts within the basins are closely correlated with these zones that have been *** integrated methodology of GIS-based morphometric analysis and geomorphic study enables the identification of deformed landforms associated with ongoing tectonic ***,these results offer valuable insights for informing watershed management strategies and promoting sustainable land use planning initiatives.

关键词： Nandakini Watershed Morphotectonic indices Steepness indices Active tectonics Relative active tectonic index(IRAT)

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Higher abundance of DLD protein in buffalobull spermatozoa causes elevated ROSproduction leading to early sperm capacitationand reduction in fertilizing ability

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Journal of Animal Science and Biotechnology 2025年第1期16卷 107-120页

作者： Seema Karanwal Ankit Pal Fanny Josan Aditya Patel Jatinder singh Chera Sonam Yadav Vikrant Gaur Preeti Verma Shiva Badrhan Vitika chauhan Mukesh Bhakat Tirtha Kumar Datta Rakesh Kumar Animal Genomics Laboratory Animal Biotechnology DivisionICAR-National Dairy Research InstituteKarnalHaryanaIndia NBGC Laboratory National Bureau of Animal Genetics ResourcesKarnalHaryanaIndia Artificial Breeding Research Centre LPM DivisionICAR-National Dairy Research InstituteKarnalHaryanaIndia ICAR-Central Institute of Research On Goat MakhdoomMathuraUttar PradeshIndia ICAR-Central Institute for Research On Buffaloes HisarHaryanaIndia

Backgroud Before fertilization,spermatozoa undergo a crucial maturation step called capacitation,which is a unique event regulates the sperm’s ability for successful *** capacitation process takes place as the spermatozoa pass through the female reproductive tract(FRT).Dihydrolipoamide dehydrogenase(DLD)protein is a post-pyruvate metabolic enzyme,exhibiting reactive oxygen species(ROS)production which causes ***,other vital functions of DLD in buffalo spermatozoa are hyperactivation and acrosome *** produces the optimum amount of ROS required to induce capacitation process in *** on physiological or patho-physiological conditions,DLD can either enhance or attenuate the production of reactive oxygen species(ROS).Aim of this study was to investigate whether changes in the production of ROS in sperm cells can impact their ability to fertilize by triggering the capacitation and acrosome *** In this study,abundance of DLD protein was quantified between high(n=5)and low fertile bull(n=5)*** was found that compared to high-fertile(HF)bulls,low-fertile(LF)bulls exhibited significantly(P<0.05)higher DLD ***,we optimised the MICA concentration to inhibit DLD function,spermatozoa were treated with MICA in time(0,1,2,3,4,and 5 h)and concentrations(1,2.5,5,and 10 mmol/L)dependent *** DLD inhibition was found to be at 4 h in 10 mmol/L MICA concentration,which was used for further exper-imentation in HF and *** on DLD inhibition it was seen that LF bull spermatozoa exhibited significantly(P<0.05)higher ROS production and acrosome reaction in comparison to the HF bull *** kinematic parameters of the spermatozoa such as percent total motility,velocity parameters(VCL,VSL,and VAP)and other parameters(BCF,STR,and LIN)were also decreased in MICA treated spermatozoa in comparison to the control(capacitated)*** The present study provides an initial

关键词： Acrosome reaction Capacitation High fertile bull Low fertile bull Protein Reactive oxygen species Spermatozoa

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Feasibility of Grid-connected Solar-wind Hybrid System with Electric Vehicle Charging Station

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Journal of Modern Power Systems and Clean Energy 2021年第2期9卷 295-306页

作者： Shakti singh Prachi chauhan Nirbhow Jap singh the Department of Electrical and Instrumentation Engineering Thapar Institute of Engineering and TechnologyPatialaIndia

Recently,renewable power generation and electric vehicles(EVs)have been attracting more and more attention in smart *** paper presents a grid-connected solar-wind hybrid system to supply the electrical load demand of a small shopping complex located in a university campus in ***,an EV charging station is incorporated in the *** analysis is performed for the proposed setup to satisfy the charging demand of EVs as well as the electrical load demand of the shopping *** proposed system is designed by considering the cost of the purchased energy,which is sold to the utility grid,while the power exchange is ensured between the utility grid and other components of the *** sizing of the component is performed to obtain the least levelized cost of electricity(LCOE)while minimizing the loss of power supply probability(LPSP)by using recent optimization *** results demonstrate that the LCOE and LPSP for the proposed system are measured at 0.038$/k Wh and0.19%with a renewable fraction of 0.87,*** is determined that a cost-effective and reliable system can be designed by the proper management of renewable power generation and load *** proposed system may be helpful in reducing the reliance on the over-burdened grid,particularly in developing countries.

关键词： Charging station electric vehicle(EV) optimization solar photovoltaic(SPV)panels wind turbine

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State of the art in finite element approaches for milling process:a review

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Advances in Manufacturing 2023年第4期11卷 708-751页

作者： Shailendra chauhan Rajeev Trehan Ravi Pratap singh Department of Industrial and Production Engineering Dr.B R Ambedkar National Institute of TechnologyJalandharPunjabIndia

Over a century,metal cutting has been observed as a vital process in the domain of *** the numerous available metal-cutting processes,milling has been considered as one of the most employable processes to machine a variety of engineering materials *** the milling process,material removal occurs when the workpiece is fed against a rotating tool with multiple cutting *** order to maximize the profitability of metal cutting operations,it is essential that the various input and output variable relationships are analyzed and *** experimental method of studying milling processes is costly and time demanding,particularly when a large variety of elements such as cutting tool shape,materials,cutting conditions,and so on,are *** to these issues,other alternatives emerged in the form of mathematical simulations that employ numerical *** finite element approaches have well-proven to be the most practical and commonly utilized numerical *** finite element model(FEM)can be used to determine the various physical interactions occurring during the machining process along with the prediction of various milling characteristics,such as cutting forces,cutting temperature,stresses,etc.,with the help of milling *** the present article,various research studies in the broad milling process domain practiced with numerous finite element approaches have been critically reviewed and *** further highlights the several experimental and finite element approaches-based research studies that attempted to analyze and optimize the overall performance of the different milling *** recent years,various investigators have explored numerous ways to enhance milling performance by probing the different factors that influence the quality *** of the studies have also been found to be focused on the economic impacts of milling and various process inputs that affect milling ***,various milli

关键词： Milling Finite element modeling(FEM) Micro milling Turn-milling

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Sarve:synthetic data and local differential privacy for private frequency estimation

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Cybersecurity 2022年第4期5卷 97-116页

作者： Gatha Varmal Ritu chauhan Dhananjay singh Amity Institute of Information Technology Amity UniversityNoidaIndia Center for Computational Biology and Bioinformatics Amity UniversityNoidaIndia Department of Electronics Engineering HUFSSeoulKorea

The collection of user attributes by service providers is a double-edged *** are instrumental in driving statistical analysis to train more accurate predictive models like *** analysis of the collected user data includes frequency estimation for categorical ***,the users deserve privacy guarantees against inadvertent identity *** algorithms called frequency oracles were developed to randomize or perturb user attributes and estimate the frequencies of their *** propose Sarve,a frequency oracle that used Randomized Aggregatable Privacy-Preserving Ordinal Response(RAPPOR)and Hadamard Response(HR)for randomization in combination with fake *** design of a service-oriented architecture must consider two types of complexities,namely computational and *** functions of such systems aim to minimize the two complexities and therefore,the choice of privacy-enhancing methods must be a calculated *** variant of RAPPOR we had used was realized through bloom flters.A bloom filter is a memory-efficient data structure that offers time complexity of O(1).On the other hand,HR has been proven to give the best communication costs of the order of log(b)for b-bits ***,Sarve is a step towards frequency oracles that exhibit how privacy provisions of existing methods can be combined with those of fake data to achieve statistical results comparable to the original *** also implemented an adaptive solution enhanced from the work of Arcolezi et *** use of RAPPOR was found to provide better privacy-utility tradeoffs for specific privacy budgets in both high and general privacyregimes.

关键词： Synthetic data Differential privacy Frequency estimation Frequency oracle Privacy

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Microplastics removal strategies:A step toward finding the solution

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Frontiers of Environmental Science & Engineering 2022年第1期16卷 19-36页

作者： Neha Badola Ashish Bahuguna Yoel Sasson Jaspal singh chauhan Aquatic Biodiversity Lab Department of Himalayan Aquatic BiodiversityHemvati Nandan Bahuguna Garhwal University(A Central University)Srinagar-GarhwalUttarakhand 246174India Casali Center for Applied Chemistry Institute of ChemistryThe Hebrew University of Jerusalem9190401Israel Department of Chemistry Uttaranchal UniversityDehradunUttarakhand 248007India

Microplastics are an emerging threat and a big challenge for the *** presence of microplastics(MPs)in water is life-threatening to diverse organisms of aquatic ***,the scientific community is exploring deeper to find treatment and removal options of *** physical,chemical and biological methods are researched for MPs removal,among which few have shown good efficiency in the *** methods also have a few limitations in environmental *** than finding a suitable method,the creation of legal restrictions at a governmental level by imposing policies against MPs is still a daunting task in many *** review is an effort to place all effectual MP removal methods in one document to compare the mechanisms,efficiency,advantages,and disadvantages and find the best ***,it also discusses the policies and regulations available in different countries to design an effective global *** are also made to discuss the research gaps,recent advancements,and insights in the field.

关键词： Aquatic Coagulation Microplastics Plastic Water Treatment Plant Wastewater

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Design of computer vision assisted machine learning based controller for the Stewart platform to track spatial objects

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Frontiers of Structural and Civil Engineering 2024年第8期18卷 1195-1208页

作者： Dev Kunwar singh chauhan Pandu R.VUNDAVILLI Indian Institute of Technology Bhubaneswar Bhubaneswar 752050India

The present work aims to develop an object tracking controller for the Stewart platform using a computer vision-assisted machine learning-based *** research is divided into two *** first module focuses on the design of a motion controller for the Physik Instrumente(PI)-based Stewart *** contrast,the second module deals with the development of a machine-learning-based spatial object tracking algorithm by collecting information from the Zed 2 stereo vision ***,simple feed-forward neural networks(NN)are used to predict the orientation of the top table of the *** training,the x,y,and z coordinates of the three-dimensional(3D)object,extracted from images,are used as the input to the *** contrast,the orientation information of the platform(that is,rotation about the x,y,and z-axes)is considered as the output from the *** orientation information obtained from the network is fed to the inverse kinematics-based motion controller(module 1)to move the platform while tracking the *** training,the optimised NN is used to track the continuously moving 3D *** experimental results show that the developed NN-based controller has successfully tracked the moving spatial object with reasonably good accuracy.

关键词： Stewart platform feed-forward neural networks motion controller inverse kinematics stereo vision

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