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Time course of physiological,biochemical,and gene expression changes under short-term salt stress in Brassica juncea L.

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The Crop Journal 2017年第3期5卷 219-230页

作者： manish pandey Suprasanna Penna Plant Stress Physiology and Biotechnology Section Nuclear Agriculture and Biotechnology DivisionBhabha Atomic Research CentreTrombayMumbai 400085India

Salinity-imposed limitations on plant growth are manifested through osmotic and ionic imbalances. However, because salinity-induced responses vary considerably among crop plants, monitoring of such responses at an early stage has relevance. In this study,physiological(seed germination, seed vigor index, root length, shoot length, fresh weight,dry weight) and biochemical attributes(osmoprotectants, K^+/Na^+ ratio) were analyzed for a time-course assessment of salt responses in Indian mustard(Brassica juncea L.) with an emphasis on early monitoring. The results showed strong correlations for total soluble sugars at germination phase(24 h), proline content in the seedling establishment phase(48 h) and various physiological parameters including seed vigor index(R^2= 0.901), shoot length(R^2= 0.982), and fresh weight(R^2= 0.980) at 72 h(adaptation under stress). In addition, transcriptional changes were observed under NaCl treatment for key genes belonging to the family of selective ion transporters(NHX, HKT) and abscisic acid synthesis(AAO-3). The status of mitochondrial respiration was also examined as a probe for salinity tolerance at an early stage. The results suggested that although all the analyzed parameters showed correlations(negative or positive) with salt stress magnitude, their critical response times differed, with most of the studied biochemical, physiological, or molecular markers providing valuable information only after radicle emergence, whereas mitochondrial respiration via alternative oxidase was useful for the early detection of salt responses.

关键词： Alternative oxidase Brassica Osmolytes Salinity Seed germination

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Influence of cohesion on California bearing ratio of clay-gravel mixtures

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International Journal of Sediment Research 2023年第3期38卷 374-386页

作者： Rajesh Jain Ketan Timani manish pandey Civil Engineering Department L.D.College of Engineering 2. Vishwakarma Government Engineering College Department of Civil Engineering National Institute of Technology

An understanding of the behavior of cohesive sediment is required to solve various engineering problems such as scour around bridge elements, mitigation of soil erosion, pavement design, river bed degradation,stable channel design. Pavement foundation designers principally use the California bearing ratio(CBR)to describe the subgrade and subbase materials and their strength. Several laboratory experiments were done to study the variation in the CBR of cohesive mixtures comprised of clay-gravel mixtures. Nine different clay-gravel mixtures were used in which the clay content varies from 10% to 50% by *** variation of the CBR with clay percentage, moisture content, and undrained shear strength parameters was studied. The CBR value reduces with the increase in the moisture content and clay fraction in the mixtures and increases with an increase in the dry density of the mixture under unsoaked conditions. The CBR also increases with the increase of the angle of internal friction of clay-gravel mixtures.A functional relation has been identified to estimate the CBR of clay-gravel mixtures. Using multiple linear regression analysis(MLRA), a relation is proposed to estimate the CBR of clay-gravel mixtures under unsoaked conditions. A statistical analysis was done to judge the behavior of the pertinent variables on the CBR. The proposed relation predicts the CBR of clay-gravel mixtures very well. Artificial neural network(ANN) analysis using R programming was also done to determine the effects of the pertinent variables on the CBR. ANN methodology was applied to predict the contribution of each variable. Three different methods: Garson algorithm, Olden algorithm, and Lek’s profile model are used to assess the influence of variable parameters. The Olden algorithm and Lek’s profile both show positive association of cohesion with CBR in an unsoaked condition. The role of moisture content was found to be marginally negative in the Olden algorithm and Lek’s profile results

关键词： Clay–gravel mixture Cohesive sediment California bearing ratio Clay percentage Sediment properties

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Flow turbulence presented by different vegetation spacing sizes within a submerged vegetation patch

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Journal of Hydrodynamics 2023年第6期35卷 1131-1145页

作者： Chukwuemeka Kingsley John Jaan H.Pu Yakun Guo Prashanth R.Hanmaiahgari manish pandey Faculty of Engineering and Informatics University of BradfordBradfordUK Department of Civil Engineering Indian Institute of TechnologyKharagpurIndia National Institute of Technology(NIT) WarangalIndia

This study presents results from a vegetation-induced flow experimental study which investigates 3-D turbulence structure profiles,including Reynolds stress,turbulence intensity and bursting analysis of open channel *** vegetation densities have been built between the adjacent vegetations,and the flow measurements are taken using acoustic Doppler velocimeter(ADV)at the locations within and downstream of the vegetation *** different tests are conducted,where the first test has compact vegetations,while the second and the third tests have open spaces created by one and two empty vegetation slots within the vegetated *** reveals that over 10%of eddies size is generated within the vegetated zone of compact vegetations as compared with the fewer *** turbulence structures variation is also observed at the points in the non-vegetated *** findings from burst-cycle analysis show that the sweep and outward interaction events are dominant,where they further increase away from the *** effect of vegetation on the turbulent burst cycle is mostly obvious up to approximately two-third of vegetation height where this phenomenon is also observed for most other turbulent structure.

关键词： Velocity profiles acoustic Doppler velocimeter(ADV) turbulent intensities Reynolds stress turbulent bursting vegetated flow vegetation spacing sizes

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Experimental assessment and prediction of temporal scour depth around a spur dike

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International Journal of Sediment Research 2021年第1期36卷 17-28页

作者： manish pandey Manousos Valyrakis Meilan Qi Anurag Sharma Ajay Singh Lodhi Department of Civil Engineering National Institute of Technology School of Engineering University of Glasgow School of Civil Engineering Beijing Jiaotong University Department of Civil Engineering Institute of Infrastructure Technology Research and Management 5. College of Agriculture Waraseoni District

Spur dikes are river training structures that have been extensively used worldwide for towards enhancing flood control and the stability of embankments and ***,scour around spur dikes can be a major problem affecting their stability and hydraulic *** precise computation of temporal scour depth at spur dikes is very important for the design of economical and safe spur *** study focuses on experimentally assessing the temporal variation of scour depth around a vertical wall spur dike and identifying the parameters,which mostly influence spur dike performance for a channel bed surface comprised of sand-gravel *** the current study,the authors did physical experiments in a flume based study to obtain new data,aimed at deriving a new predictive model for spur dike scour and comparing its performance to others found in the *** was found that the dimensionless temporal scour depth variation increases with an increase in(i) the threshold velocity ratio,(ii) the densimetric Froude number of the bed surface sediment mixture,(iii) the flow shallowness(defined as the ratio of the approach flow depth,y,to the spur dike’s transverse length,l),and(iv) the flow depth-particle size *** is also concluded that the temporal scour depth variation in the sediment mixture is influenced by the non-uniformity of sediment and decreases with an increase in the non-uniformity of the sediment mixture.A new mathematical model is derived for the estimation of temporal scour depths in sand-gravel sediment *** proposed equation has been calibrated and validated with the experimental data,demonstrating a good predictive capacity for the estimation of temporal scour depth evolution.

关键词： Spur dike Sand-gravel sediment mixture Clear-water scour Time-dependent scour depth

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Identification of ExpIdentification of Expressed Resistance Gene Analogs from Peanut (Arachis hypogaea L.) Expressed Sequence Tags

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Journal of Integrative Plant Biology 2013年第5期55卷 453-461页

作者： Zhanji Liu Suping Feng manish K. pandey Xiaoping Chen Albert K. Culbreath Rajeev K. Varshney Baozhu Guo University of Georgia Department of Plant Pathology Shandong Academy of Agricultural Sciences High-Tech Research Center Qiongzhou University College of Bioscience and Biotechnology International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) Guangdong Academy of Agricultural Sciences Crops Research Institute US Department of Agriculture Agricultural Research ServiceCrop Protection and Management Research Unit

Low genetic diversity makes peanut （Arachis hypogaea L.） very vulnerable to plant pathogens, causing severe yield loss and reduced seed quality. Several hundred partial genomic DNA sequences as nucleotide-binding-site leucine-rich repeat （NBS-LRR） resistance genes （R） have been identified, but a small portion with expressed transcripts has been found. We aimed to identify resistance gene analogs （RGAs） from peanut expressed sequence tags （ESTs） and to develop polymorphic markers. The protein sequences of 54 known R genes were used to identify homologs from peanut ESTs from public databases. A total of 1,053 ESTs corresponding to six different classes of known R genes were recovered, and assembled 156 contigs and 229 singletons as peanut-expressed RGAs. There were 69 that encoded for NBS-LRR proteins, 191 that encoded for protein kinases, 82 that encoded for LRR-PK/transmembrane proteins, 28 that encoded for Toxin reductases, 11 that encoded for LRR-domain containing proteins and four that encoded for TM-domain containing proteins. Twenty-eight simple sequence repeats （SSRs） were identified from 25 peanut expressed RGAs. One SSR polymorphic marker （RGA121） was identified. Two polymerase chain reaction-based markers （Ahsw-1 and Ahsw-2） developed from RGA013 were homologous to the Tomato Spotted Wilt Virus （TSWV） resistance gene. All three markers were mapped on the same linkage group AhIV. These expressed RGAs are the source for RGA-tagged marker development and identification of peanut resistance genes.

关键词： Arachis hypogaea expressed sequence tags resistance gene analogs Tomato Spotted Wilt Virus.

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Landslide Susceptibility Mapping Using RBFN-Based Ensemble Machine Learning Models

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CMES-COMPUTER MODELING IN ENGINEERING & SCIENCES 2024年第1期142卷 467-500页

作者： Nguyen, Duc-Dam Tiep, Nguyen Viet Bui, Quynh-Anh Thi Van Le, Hiep Prakash, Indra Costache, Romulus pandey, manish Pham, Binh Thai Univ Transport Technol Fac Civil Engn Hanoi 100000 Vietnam Hanoi Univ Civil Engn Fac Hydraul Engn Hanoi 100000 Vietnam DDG R Geol Survey India Gandhinagar 382010 India Univ Bucharest Res Inst Bucharest 050663 Romania Natl Inst Hydrol & Water Management Bucharest 013686 Romania Transilvania Univ Brasov Dept Civil Engn Brasov 500152 Romania Danube Delta Natl Inst Res & Dev Tulcea 820112 Romania Marwadi Univ Rajkot 360003 Gujarat India Marwadi Univ Fac Engn & Technol Dept Civil Engn Rajkot 360003 Gujarat India

This study was aimed to prepare landslide susceptibility maps for the Pithoragarh district in Uttarakhand, India, using advanced ensemble models that combined Radial Basis Function Networks (RBFN) with three ensemble learning techniques: DAGGING (DG), MULTIBOOST (MB), and ADABOOST (AB). This combination resulted in three distinct ensemble models: DG-RBFN, MB-RBFN, and AB-RBFN. Additionally, a traditional weighted method, Information Value (IV), and a benchmark machine learning (ML) model, Multilayer Perceptron Neural Network (MLP), were employed for comparison and validation. The models were developed using ten landslide conditioning factors, which included slope, aspect, elevation, curvature, land cover, geomorphology, overburden depth, lithology, distance to rivers and distance to roads. These factors were instrumental in predicting the output variable, which was the probability of landslide occurrence. Statistical analysis of the models' performance indicated that the DG-RBFN model, with an Area Under ROC Curve (AUC) of 0.931, outperformed the other models. The AB-RBFN model achieved an AUC of 0.929, the MB-RBFN model had an AUC of 0.913, and the MLP model recorded an AUC of 0.926. These results suggest that the advanced ensemble ML model DG-RBFN was more accurate than traditional statistical model, single MLP model, and other ensemble models in preparing trustworthy landslide susceptibility maps, thereby enhancing land use planning and decision-making.

关键词： Landslide susceptibility map spatial analysis ensemble modelling information values (IV)

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Improvement of three popular Indian groundnut varieties for foliar disease resistance and high oleic acid using SSR markers and SNP array in marker-assisted backcrossing

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The Crop Journal 2020年第1期8卷 1-15页

作者： Yaduru Shasidhar Murali T.Variath manish K.Vishwakarma Surendra S.Manohar Sunil S.Gangurde Manda Sriswathi Hari Kishan Sudini Keshavji L.Dobariya Sandip K.Bera Thankappan Radhakrishnan manish K.pandey Pasupuleti Janila Rajeev K.Varshney International Crops Research Institute for the Semi-Arid Tropics(ICRISAT) HyderabadIndia Department of Genetics Osmania UniversityHyderabadIndia Junagadh Agricultural University JunagadhIndia ICAR-Directorate of Groundnut Research JunagadhIndia

Foliar fungal diseases(rust and late leaf spot)incur large yield losses,in addition to the deterioration of fodder quality in groundnut *** oleic acid has emerged as a key market trait in groundnut,as it increases the shelf life of the produce/products in addition to providing health benefits to ***-assisted backcrossing(MABC)is the most successful approach to introgressing or pyramiding one or more traits using traitlinked *** used MABC to improve three popular Indian cultivars(GJG 9,GG 20,and GJGHPS 1)for foliar disease resistance(FDR)and high oleic acid content.A total of 22 BC3F4 and 30 BC2F4 introgression lines(ILs)for FDR and 46 BC3F4 and 41 BC2F4 ILs for high oleic acid were *** parent genome analysis using the 58 K Axiom_Arachis array identified several lines showing upto 94%of genome recovery among second and third backcross *** of these ILs revealed FDR scores comparable to the resistant parent,GPBD 4,and ILs with high(~80%)oleic acid in addition to high genome *** ILs provide further opportunities for pyramiding FDR and high oleic acid in all three genetic backgrounds as well as for conducting multi-location yield trials for further evaluation and release for cultivation in target regions of India.

关键词： Foliar disease resistance High oleic acid Late leaf spot Marker-assisted backcrossing SNP array Background genome recovery

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Diversification of primary gene pool through introgression of resistance to foliar diseases from synthetic amphidiploids to cultivated groundnut(Arachis hypogaea L.)

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The Crop Journal 2014年第Z1期2卷 110-119页

作者： Varsha Kumari M.V.C.Gowda Vinod Tasiwal manish K.pandey Ramesh S.Bhat Nalini Mallikarjuna Hari D.Upadhyaya Rajeev K.Varshney Department of Genetics and Plant Breeding University of Agricultural Sciences Dharwad International Crops Research Institute for the Semi-Arid Tropics Department of Plant Pathology University of Agricultural Sciences Dharwad Department of Biotechnology University of Agricultural Sciences Dharwad CGIAR Generation Challenge Programme c/o CIMMYT

Groundnut(Arachis hypogaea L.)is widely grown and consumed around the world and is considered to have originated from a single hybridization event between two wild *** utilization of wild germplasm in breeding programs has been restricted by reproductive barriers between wild and cultivated species and technical difficulties in making large numbers of *** to overcome these hurdles have resulted in the development of synthetic amphidiploids,namely ISATGR 278-18(Arachis duranesis×Arachis batizocoi)and ISATGR 5B(Arachis magna×***),which possess several desirable traits,including resistance to foliar diseases that generally cause huge yield losses annually in groundnut growing areas of Asia,America,and *** an objective to improve foliar disease resistance,the primary gene pool was diversified by introgressing foliar disease resistance in five cultivated genotypes(ICGV 91114,ICGS 76,ICGV 91278,JL 24,and DH 86)from synthetic amphidiploids using a backcross breeding *** introgression lines with resistance to two foliar diseases(rust and late leaf spot)were identified with levels of resistance equal to the *** backcross derived lines have shown a wide range of variation for several morphological and agronomic *** lines,after further evaluation and selection,can serve as donors in future breeding programs aimed atdeveloping improved cultivars with desirable agronomic traits,high resilience to biotic/abiotic stresses and a broadened genetic base.

关键词： Groundnut Peanut Wild species Wide crosses Interspecific hybridization

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Atypical Olfactory Neuroblastoma Presenting as Sinonasal Polyposis with Nasopharyngeal Extension: A Case Report

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International Journal of Otolaryngology and Head & Neck Surgery 2015年第5期4卷 394-400页

作者： Jagdish Prasad Purohit Chandra Bhan Bhoopendra Singh Ajay Pratap Singh Siva Selvaraj manish pandey Vijay Krishna Sharma Department of ENT Head and Neck Surgery MLB Medical College Jhansi India

Background: Olfactory neuroblastoma also known as Esthesioneuroblastoma (ENB) is a tumor arising from the basal layer of olfactory epithelium in the superior recess of the nasal cavity in the region of cribriform plate. Incidence peaks once in 11 - 20 years of age and again in 50 - 60 years of age. It is equally found in men and women. Aim: The main aim of this case report is to characterize the clinical features of ENB showing nasopharyngeal involvement and its importance in the differential diagnosis of sinonasal neuroendocrine malignancies. Case presentation: Two cases are reported. The first case was a 21-year-old male with symptoms of nasal obstruction, recurrent epistaxis, nasal discharge, headache and paresthesia over the face with an evolution of 1 year with no previous history of trauma. Diagnostic nasal endoscopy revealed reddish gray mass filling bilateral nasal cavities. Posterior rhinoscopy revealed mass coming through choanae and filling the nasopharynx. Endoscopic resection of tumor was done and postoperatively all the symptoms of the patient resolved. The second case was a 62-year-old male with complaints of nasal obstruction, nasal discharge, recurrent epistaxis, anosmia and difficulty in swallowing. On computed tomography, anteriorly, the mass was extending up to external nares and posteriorly up to the nasopharynx, superiorly up to nasal roof and inferiorly up to hard palate. No obvious erosion of floor of anterior cranial fossa was seen. No intracranial extension was seen. Surgery was advised but patient refused to undergo any treatment. Conclusion: This study highlights the characteristics and clinical features of ENB showing nasopharyngeal involvement and their importance in the differential diagnosis of sinonasal neuroendocrine malignancies.

关键词： Esthesioneuroblastoma Cribriform Plate Neuroectodermal

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Tumour Associated Tissue Eosinophilia as a Prognostic Indicator in Squamous Cell Carcinoma of Upper Aerodigestive Tract

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International Journal of Otolaryngology and Head & Neck Surgery 2016年第2期5卷 54-58页

作者： Chandra Bhan Kartikeya Purohit Jagdish Prasad Purohit Ajay Pratap Singh Bhoopendra Singh Siva Selvaraj manish pandey Vineet Kumar Vineet Kumar Department of ENT Head and Neck Surgery MLB Medical College Jhansi India

Immune response is known to develop against malignant tumours. Malignant tumours express newer antigen on their cell surface membrane which elicit immunological reaction in and around tumoural tissue. In early part of immune reaction neutrophil, eosinophil migrates followed by monocyte-macrophage cell. Tumor associated tissue eosinophilia (TATE) is believed to play a significant role in the biological behavior of the carcinoma. Eosinophil infiltrate in association with the head and neck squamous cell carcinoma (SCC) have been reviewed from time-to-time. The significance of such an association has been variably thought to be either a potential diagnostic tool for stromal invasion or as a prognostic indicator. The aim of this study was to investigate and evaluate the possible role of the tumour associated tissue eosinophilia (TATE) as a predictive indicator for the grading and establishing prognosis of the upper aerodigestive tract squamous cell carcinoma (SCC).

关键词： Tumour Associated Tissue Eosinophilia (TATE) Squamous Cell Carcinoma (SCC)

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