检索结果-南通市图书馆

作者： caleb manamik breria 南京农业大学

学位级别：硕士

China's production of indica hybrid rice accounts for more than16million hectares per year, which makes up52%of the total cultivated rice area. Japonica rice cultivation only covers8.28million hectares per annum making up another27%of the total rice acreage. Japonica hybrid rice acreage is3%of the total japonica rice acreage as compared to indica hybrid rice. Main reasons for the slow development of hybrid japonica rice were due to its properties of weaker competitive advantages and lower seed production. Improvement of hybrid japonica rice lines is very critical in China as there are reasonable comparative advantage between indica rice production and japonica rice production. Although research on the utilization of heterosis in rice has made tremendous gains during the last30years, it is, from a strategic point of view, still in its infancy because the outcrossing characteristic potential has not been fully tapped yet. Improving japonica seed production is very vital in China. In order for higher seed productions in japonica rice, characteristics of out-crossing related traits in rice must be well studied and understood. A variety with good stigma traits and good architectural traits would have an advantage of giving high and stable F1seed production yield. Therefore, genetic and breeding studies at this level provide a platform for increase hybrid japonica seeds production.For the experiment on the evaluation procedure, a total of462different rice varieties from Taihu Lake and other areas were used as testing materials. Investigative study was done on the following traits:flag leaf angle, filament length, stigma length, anther length and percentage exerted stigma. For the genotyping,263SSR molecular markers covering the12chromosomes of rice were used to screen the462rice varieties. TASSEL software General Linear Model (GLM) was used to identify association between markers and traits. The results obtained are as follows: (1) Percentage Exerted Stigma record

关键词： out-crossing traits elite alleles association analysis polymorphism linkage disequilibrium

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Development and Application of SCAR Markers for Discriminating Cytoplasmic Male Sterile Lines from Their Cognate Maintainer Lines in Indica Rice

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Rice science 2013年第3期20卷 191-199页

作者： LU Chao LIU Jian JIANG Jian-hua caleb manamik breria TAN He-lin Masahiko ICHII HONG De-lin State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Faculty of Agriculture Kagawa University

The DNAfragments about 1 600 bp were amplified using random amplified polymorphism DNA （RAPD） primer OPAl2 with the templates of mitochondrial DNA of Zhenshan 97A and Zhenshan 97B, and were sequenced. The nucleotide sequences and lengths of the fragments from Zhenshan 97A and Zhenshan 97B showed no difference. The precise length of the fragment was 1 588 bp. Sequence characterized amplification region （SCAR） primers were then developed to discriminate the cytoplasmic male sterile （CMS） lines and their maintainer lines. A specific 1 588 bp fragment could be amplified with SCAR primers, CHI19F2/CHI19R2 and CHI20F3/CHI23R3, in the mitochondrial DNA of Zhenshan 97A, but not Zhenshan 97B. Furthermore, the specific fragment could be also amplified from the total DNA from green leaf tissues of Zhenshan 97A with SCAR primers, but not Zhenshan 97B. With the corresponding primers, the specific fragment could also be amplified from the total DNA of green leaves of other two CMS lines with wild abortive type cytoplasm （CMS-WA）, namely Zhenpin A and Tianfeng A, but not in their maintainer lines. Moreover, using total DNA as template, each of the four pairs of SCAR primers could also be used to amplify the 1 588 bp fragment in CMS-ID （Indonesia paddy type） line 11-32A but not in 11-32B, and the specific fragment was amplified from the DNA of both F1 and F2 seedlings of Shanyou 63. The results of detecting the genetic purity of a man-made mixture of the seeds of Zhenshan 97A using CHI20F3/CHI23R3 were completely consistent with the phenotypes. Taken together, these results indicated that the specific 1 588 bp-fragment amplified by CHI20F3/CHI23R3 was the unique amplification products of CMS mitochondrial DNA, and could be used to distinguish CMS-WA and CMS-ID lines from their corresponding maintainer lines at the seedling stage.

关键词： Oryza safiva cytoplasmic male sterile line maintainer line genetic purity sequence characterized amplification region marker

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粳稻杂种优势遗传基础剖析

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作物学报 2012年第12期38卷 2147-2161页

作者：江建华刘强明卢超张红刘晓丽党小景牛付安 manamik caleb breria 赵凯铭洪德林南京农业大学/作物遗传与种质创新国家重点实验室江苏南京210095 安徽省农业科学院作物研究所安徽合肥230031

为了解控制粳稻产量相关性状及其中亲优势的基因作用类型,利用秀堡RIL群体及其2个回交（BCF1）群体对株高、生育期、单株有效穗数、穗长、每穗颖花数、结实率、一次枝梗数和二次枝梗数8个性状及其中亲杂种优势进行QTL定位。共检测到58... 详细信息

为了解控制粳稻产量相关性状及其中亲优势的基因作用类型,利用秀堡RIL群体及其2个回交（BCF1）群体对株高、生育期、单株有效穗数、穗长、每穗颖花数、结实率、一次枝梗数和二次枝梗数8个性状及其中亲杂种优势进行QTL定位。共检测到58个显著的主效QTL（M-QTL）,单个M-QTL的贡献率变幅为3.3%~41.9%。77.6%的M-QTL表现为加性效应,15.5%的M-QTL表现为部分或完全显性效应,6.9%的M-QTL表现为超显性效应。共检测到90对显著的双基因上位性QTL（E-QTL）。在RIL群体中检测到44对E-QTL,单对E-QTL的贡献率变幅为1.7%~8.0%,平均3.7%。在XSBCF1群体中检测到27对E-QTL,其中利用BCF1表型值检测到16对E-QTL,单对E-QTL的贡献率变幅为12.7%~78.5%,平均29.2%;利用中亲优势值检测到11对E-QTL,单对E-QTL的贡献率变幅为15.0%~71.8%,平均40.1%。在CBBCF1群体中检测到19对E-QTL,其中利用BCF1表型值检测到12对E-QTL,单对E-QTL的贡献率变幅为2.7%~64.4%,平均30.1%;利用中亲优势值检测到9对E-QTL,单对E-QTL的贡献率变幅为21.7%~64.1%,平均40.0%。在CBBCF1群体中,利用BCF1表型值和中亲优势值都检测到的E-QTL有2对。上述结果表明上位性效应是粳稻秀堡组合杂种优势的主要遗传基础。

关键词：粳稻主效QTL 上位性QTL 杂种优势

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