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Production, purification and characterization of D-aspartate oxidase from the fungus Trichoderma harzianumSKW-36

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Advances in Bioscience and Biotechnology 2012年第1期3卷 7-13页

作者： Shigekazu Yano Hiroyuki Ozaki Shinji Matsuo Masahiro Ito mamoru Wakayama Department of Biotechnology Faculty of Life Sciences Ritsumeikan University Kusatsu Shiga Japan

Trichoderma harzianum Rifai SKW-36 produced two kinds of D-amino acid oxidizing enzymes. One enzyme was D-aspartate oxidase acting on acidic D-amino acids such as D-aspartate and D-glutamate and another one was D-amino acid oxidase acting on neutral D-amino acid such as D-phenylalanine and D-methionine. These enzymes in the cell-free extract were separated by DEAE-Toyopearl ion-exchange column chromatography. Casamino acids, peptone, and yeast extract as carbon and nitrogen sources were effective for the production of the enzymes. No D-amino acid tested induced the production of the enzymes. Casamino acid (0.33%) as carbon and nitrogen source gave a highest specific activity of D-aspartate oxidase among media tested. D-Aspartate oxidase, which was purified by four-step column chromatography in addition to ammonium sulfate precipitation, exhibited a subunit molecular mass of 40 kDa by SDS-PAGE analysis. D-Aspartate, D-glutamate and N-methyl-D- aspartate were oxidized as substrates with the specific activities of 7.80 U/mg, 4.90 U/mg, and 4.22 U/mg, respectively. D-Asparagine, D-glutamine, D-alanine, and D-valine were slightly oxidized. No other D- amino acids tested were inert. The enzyme exhibited relatively wide substrate specificity compared to D-aspartate oxidases reported so far. The pH and temperature optima were 7.5 - 8.0 and 35°C, respectively. The enzyme was stable at pH 6.0 - 9.0. About 75% of the enzyme activity was retained even after treating the enzyme at 50°C for 10 min. The enzyme activity was inhibited not by benzoate and tartrate, but 60% and 24% by fumarate and malonate, respectively.

关键词： D-Aspartate Oxidase Trichoderma harzianum Purification Characterization

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Can 5-mm Axial CT Images Sufficiently Depict the Hyperdense Middle Cerebral Artery Sign in Patients with Acute Brain Ischemia?

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Open Journal of Radiology 2013年第4期3卷 196-200页

作者： Tomoya Nakatsuka mamoru Kurotsuchi Hideo Morita Tsutomu Inaoka Hitoshi Terada Department of Radiology Toho University Sakura Medical Center Sakura Japan

The purpose of this study was to determine whether 5-mm axial CT images can sufficiently depict the hyperdense middle cerebral artery (MCA) sign in patients with acute brain ischemia. Materials and Methods: A retrospective review of 92 cases of ischemic brain infarction confirmed nine patients showing hyperdense MCA signs. CT images were acquired on a 64-slice helical scanner. Images were reconstructed into contiguous 5-mm axial, coronal and sagittal datasets. The first CT scan images of the patients with hyperdense MCA signs and an equal number of CT scans without hyperdense MCA signs inserted at random were analyzed in a blinded review. The presence of the hyperdense MCA sign in M1 segments was recorded. Round regions of interest (ROI) were placed over the M1 segments of the MCAs and the attenuation values in Hounsfield units (HU) were measured on the sagittal and axial images. Results: Nine patients showed a hyperdense MCA sign. They consisted of 4 male and 5 female (mean age, 74.3 years;age range, 45 - 88 years). On the blinded review, hyperdense MCA signs were detected on axial images in 7 patients. In four of the 7 patients, the hyperdense MCA sign was more conspicuous on sagittal images than that on axial images. Hyperdense MCA sign was detected on sagittal images of all 9 patients and exclusive to the sagittal images in two patients. The ROI study showed higher attenuation for the affected MCA on sagittal images (46 - 65 HU) than that on axial images (36 - 54 HU). Conclusion: In patients with acute brain ischemia, 5-mm axial CT images cannot sufficiently depict the hyperdense MCA sign. Sagittal images may be superior to axial images for identifying the sign.

关键词： Multidetecter CT Hyperdense MCA Sign Sagittal Reformation

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MU雷达观测到的台风

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气象科技 1992年第4期20卷 36-43,F003页

作者： Toru Sato Naoki Ao mamoru Yamamoto 封娟兰

8719号台风途经日本期间,用MU(中、高层大气)雷达对对流层和平流层低层进行了60小时的连续观测.以150m的高度分辨率每隔2.5分钟测量风速矢量的高度廓线.1987年10月17日,8719号台风袭击了日本,并在距MU雷达100km的范围内通过.18km以下都... 详细信息

8719号台风途经日本期间,用MU(中、高层大气)雷达对对流层和平流层低层进行了60小时的连续观测.以150m的高度分辨率每隔2.5分钟测量风速矢量的高度廓线.1987年10月17日,8719号台风袭击了日本,并在距MU雷达100km的范围内通过.18km以下都能观测到清晰的台风旋转结构,往上直到24km,风有短时的振动,表明受台风的强烈影响.整个观测期间,都发现有垂直波长为2-4km,周期为10小时的主波.高空风分析图显示,这些波从一个逆温层附近向上和向下传播,这意味着波动是在逆温层上生成的.

关键词：台风 MU雷达观测对流层平流层

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Geckos on the Tower of Babel:Multiple Evolutionary Loss of Acoustic Species-recognition in Gekko

Geckos on the Tower of Babel:Multiple Evolutionary Loss of A...

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四川省动物学会第十届会员代表大会暨第十一次学术研讨会

作者： Teppei JONO mamoru TODA Ding LI Yezhong TANG Chengdu Institute of Biology Chinese Academy of Sciences

Gekko japonicus group species are common nocturnal geckos in Eastern *** than 25 species of Gekko are distributed in China,Japan,Vietnam,and Laos,and they have similar ecological and morphological *** works reported that the males of these species use acoustic signals in context of *** phylogeographic study revealed that some of them co-occur without causing hybridization,whereas some species hybridize with sympatric *** suggests that reproductive isolation occurs in the former species combinations,whereas that does not work in the later species *** investigated the role of the sexualcalls for species recognition in *** recording by 18 Gekko species revealed that 14 species have species-specific patterns in male sexual calls(named pattern calls),whereas four species did not have any pattern in its calls(named patternless calls).Results of molecular phylogenetic analysis revealed that the species-specific patterns in sexual call has lost probably three *** non-hybridizing species combinations,males of both species had pattern *** which each pattern call species,females of both species discriminated conspecific calls from the other’s *** the other hand,in hybridizing combinations,males of one species have a pattern call,but the other species have a patternless *** pattern call species discriminated conspecific calls from the other’s calls,but the patternless call species did not discriminate *** results suggested that the 14 Gekko species use the species-specific pattern calls for species identification,whereas other four species do not have such acoustic species-identification *** that the distribution area of patternless species is restricted to small islands,the species identification system by calls may be lost by geographic isolation in the islands,and the hybridization is caused by the loss of species recognition system in females of the patternless call

关键词： Gekko acoustic species-recognition pattern calls patternless calls

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具有综合性特征的中国社会学——论陆学艺的社会学思想

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西北师大学报（社会科学版） 2020年第2期57卷 62-69页

作者：李升佐佐木卫北京工业大学陆学艺学术思想研究中心/北京社会管理研究基地北京100124 日本神户大学/北京工业大学首都社会建设与社会管理协同创新中心北京100124

中国社会学家陆学艺始终贯彻的学术态度可概括为两个方面,其一是切实继承中国社会学"立足社会实践"的特征,其二是努力坚持中国社会学"俯瞰社会整体"的特征。前者可以概括为俯瞰中国地域整体的实践社会学,而后者则是俯瞰整体社会结构的... 详细信息

中国社会学家陆学艺始终贯彻的学术态度可概括为两个方面,其一是切实继承中国社会学"立足社会实践"的特征,其二是努力坚持中国社会学"俯瞰社会整体"的特征。前者可以概括为俯瞰中国地域整体的实践社会学,而后者则是俯瞰整体社会结构的理论社会学。陆学艺社会学研究的思想脉络是"立足社会实践-分析社会结构-探究社会问题-面向社会改革-开展社会建设",即在"实践-理论"两个维度实现社会学中国化。基于陆学艺社会学研究的实践取向与学术旨趣,可命之为"陆学艺综合社会学",以较好地概括陆学艺的社会学中国化的方法及理论,这对于社会学中国化及中国社会学海外化具有十分重要的研究启示和价值。

关键词：陆学艺社会学思想综合社会学社会学中国化

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Diagnostic role of 18F-fluorodeoxyglucose positron emission tomography for follicular lymphoma with gastrointestinal involvement

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World Journal of Gastroenterology 2012年第44期18卷 6427-6436页

作者： Masaya Iwamuro Hiroyuki Okada Katsuyoshi Takata Katsuji Shinagawa Shigeatsu Fujiki Junji Shiode Atsushi Imagawa Masashi Araki Toshiaki Morito mamoru Nishimura Motowo Mizuno Tomoki Inaba Seiyu Suzuki Yoshinari Kawai Tadashi Yoshino Yoshiro Kawahara Akinobu Takaki Kazuhide Yamamoto Department of Gastroenterology and Hepatology Okayama University Graduate School of MedicineDentistryand Pharmaceutical SciencesOkayama 700-8558Japan Department of Endoscopy Okayama University HospitalOkayama 700-8558Japan Department of Pathology Okayama University Graduate School of MedicineDentistryand Pharmaceutical SciencesOkayama 700-8558Japan Department of Hematology and Oncology Okayama University Graduate School of MedicineDentistryand Pharmaceutical SciencesOkayama 700-8558Japan Department of Internal Medicine Tsuyama Central HospitalTsuyama 708-0841Japan Department of Internal Medicine Okayama Saiseikai General HospitalOkayama 700-8511Japan Department of Gastroenterology Mitoyo General HospitalKanonji 769-1695Japan Department of Internal Medicine Kagawa Rosai HospitalMarugame 763-8502Japan Department of Anatomic Pathology Kagawa Rosai HospitalMarugame 763-8502Japan Department of Internal Medicine Okayama Citizens' HospitalOkayama 700-8557Japan Department of Internal Medicine Hiroshima City HospitalHiroshima 730-8518Japan Department of Gastroenterology Kagawa Prefectural Central HospitalTakamatsu 760-8557Japan Department of Internal Medicine Sumitomo Besshi HospitalNiihama 792-8543Japan Department of Gastroenterology Onomichi Municipal HospitalOnomichi 722-8503Japan

AIM:To investigate the capacity for 18F-fluorodeoxyglucose(18F-FDG) positron emission tomography(PET) to evaluate patients with gastrointestinal lesions of follicular ***:This retrospective case series consisted of 41 patients with follicular lymphoma and gastrointestinal involvement who underwent 18F-FDG-PET and endoscopic evaluations at ten different institutions between November 1996 and October *** for endoscopic,radiological,and biological examinations performed were retrospectively reviewed from clinical records.A semi-quantitative analysis of 18F-FDG uptake was performed for each involved area by calculating the maximum standardized uptake value(SUVmax).Based on the positivity of 18F-FDG uptake in the gastrointestinal lesions analyzed,patients were subdivided into two *** identify potential predictive factors for 18F-FDG positivity,these two groups were compared with respect to gender,age at diagnosis of lymphoma,histopathological grade,pattern of follicular dendritic cells,mitotic rate,clinical stage,soluble interleukin-2 receptor levels detected by 18F-FDG-PET,lactate dehydrogenase(LDH) levels,hemoglobin levels,bone marrow involvement,detectability of gastrointestinal lesions by computed tomography(CT) scanning,and follicular lymphoma international prognostic index(FLIPI) ***:Involvement of follicular lymphoma in the stomach,duodenum,jejunum,ileum,cecum,colon,and rectum was identified in 1,34,6,3,2,3,and 6 patients,*** patient had esophageal *** total,19/41(46.3%) patients exhibited true-positive 18F-FDG uptake in the lesions present in their gastrointestinal *** contrast,false-negative 18F-FDG uptake was detected in 24 patients(58.5%),while false-positive 18F-FDG uptake was detected in 5 patients(12.2%).In the former case,2/19 patients had both 18F-FDG-positive lesions and 18F-FDGnegative lesions in the gastrointestinal *** patients with 18F-FDG avidity,the SUVmax value of the involved gast

关键词： Follicular lymphoma Gastrointestinal endoscopy F-fluorodeoxyglucose positron emission tomography Gastrointestinal lymphoma Duodenal neoplasm

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Two Cases of Distal Anterolateral Thigh Flap

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Surgical Science 2013年第3期4卷 189-191页

作者： Masami Saito Naohiro Kimura mamoru Tnegawa Yoichi Tomizuka Taku Tsukakoshi Masamitsu Onda Department of I-I and Tower Clinic Tokyo Japan Department of Oumu Municipal Hospital Asahikawa Japan Department of Plastic and Reconstructive Surgery Shinyurigaoka General Hospital Kawasaki Japan Department of Tsukiji Dermatology Plastic and Hand Surgery Department of Plastic and Reconstructive Surgery Tokyo Japan Tokyo Teishin Hospital Tokyo Japan

We report our experience with two cases of anterolateral thigh flaps based on the musculocutaneous perforators arising from a more distal part of the descending branch than in the conventional anterolateral thigh flap. Case 1: A 53-year-old man with a skin ulcer over the anterior surface of the left tibia secondary to plate fixation for an open fracture. The perforator marked with preoperative Doppler was located 8 cm proximal to the superior border of the patella. Without thinning, the flap was 10 × 4 cm in size and 5 mm thick. Case 2: A 46-year-old man with necrosis of the right index finger after replantation. The flap was 5 × 3 cm in size and 3 mm in thickness. This “distal” anterolateral thigh flap can be raised as a quite thin flap with a long pedicle and, therefore, is considered useful in the reconstruction of various soft tissue defects.

关键词： Anterolateral Thigh Flap Distal Thigh Flap Thin Flap

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Efficient Production of L-Theanine Using Immobilized Recombinant Escherichia coliCells Expressing a Modified γ-Glutamyltranspeptidase Gene from Pseudomonas nitroreducens

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Advances in Biological Chemistry 2020年第6期10卷 157-171页

作者： Putthapong Phumsombat Chiharu Sano Hiroki Ikezoe Junji Hayashi Takafumi Itoh Takao Hibi mamoru Wakayama Department of Biotechnology College of Life Sciences Ritsumeikan University Shiga Japan Graduate School of Technology Industrial and Social Sciences Tokushima University Tokushima Japan Department of Bioscience and Biotechnology Fukui Prefectural University Fukui Japan

L-Theanine (γ-glutamylethylamide) is a naturally occurring amino acid derivative known to have several beneficial physiological effects as a diet supplement, and to give an umami taste when used as a food additive. The compound is industrially produced by γ-glutamyltranspeptidase from Pseudomonas nitroreducens (PnGGT). Using recombinant PnGGT, we have shown previously that Trp385, Phe417, and Trp525 are key amino acid residues for recognition of acceptor substrates at the PnGGT active site. Here, we demonstrate that a recombinant W525D mutant of PnGGT produces L-theanine from ethylamine and L-glutamine more efficiently than wild-type PnGGT, attributable to an increased ratio of transfer activity to hydrolysis activity. An efficient production of L-theanine was achieved by immobilizing Escherichia coli cells expressing the W525D PnGGT mutant (E. coli-W525D) using 2% alginate as the supporting material. The highest L-theanine production using immobilized E. coli-W525D, representing a conversion rate of 90%, was achieved in optimal reaction conditions of pH 10, 40°C, and a substrate molar ratio of L-glutamine to ethylamine of 1:10. The immobilized E. co

关键词： L-Theanine γ-Glutamyltranspeptidase Pseudomonas nitroreducens Escherichia coli Immobilization

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利用近红外彩色成像技术监测松萎蔫病

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南京林业大学学报（自然科学版） 2011年第4期35卷 150-151页

作者： Katsunori Nakamura mamoru Takehana Tsuneo Itagaki Hayato Tashiro Kazumasa Ohta Osamu Nakakita Tohoku Research Center Forestry and Forest Products Research Institute Morioka Iwate 020-0123 Japan

有效监测患病株方法的缺失是导致对松萎蔫病难以成功控制的主要原因之一。利用航空手段调查该病导致的针叶颜色变化已被证明是一种可靠的方法。为了克服该方法固有的缺点,笔者引用了近红外彩色胶片航拍方法。该项技术在获得正摄航拍图像... 详细信息

有效监测患病株方法的缺失是导致对松萎蔫病难以成功控制的主要原因之一。利用航空手段调查该病导致的针叶颜色变化已被证明是一种可靠的方法。为了克服该方法固有的缺点,笔者引用了近红外彩色胶片航拍方法。该项技术在获得正摄航拍图像时,通过图像处理可以增强松针颜色变化,获得较直接拍摄图像更明显的色差。因为图像可以在室内仔细处理,因此能解决飞行时间缺乏等问题。但是,该方法也存在对被压木无法获得其影像而漏检的缺陷。笔者研发的计算机软件可以将目标树标记在计算机图像文件上,并且标记树的地理位置数据和背景航拍图像并传输到装有内置GPS接收器的掌上电脑中,借助软件产生的图像导航定位系统,有利于地面接近标记树,从而可以现场检查和校正标记树的数据,并将修正数据传回至主机。此方法利用最新航拍图像技术建立对每株树处理和管理的连续资料,大大改善了防控松萎蔫病的措施。

关键词：松萎蔫病红外航空图像地理信息系统监测

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Production of Bioethanol at High Temperature from Tari

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Advances in Microbiology 2015年第5期5卷 325-335页

作者： Ali Azam Talukder Sirajul Islam Sujon Muhammad Maqsud Hossain Donald James Gomes mamoru Yamada Department of Microbiology Jahangirnagar University Dhaka Bangladesh Department of Microbiology North South University Dhaka Bangladesh Department of Biological Chemistry Yamaguchi University Yamaguchi Japan Department of Microbiology Dhaka University Dhaka Bangladesh

Many microorganisms can tolerate high-temperature ranges from 37°C - 45°C are called thermotolerant microorganisms. Eighteen such isolates containing various microorganisms were collected from the natural fermented products of Bangladesh in summer for bioethanol production. Cultural, morphological, physiological, biochemical and genetical analysis were carried out under various physiological conditions. Among them, two thermotolerant strains Tari-6, isolated from the Tari (an overnight natural fermented palm juice at around 33°C - 40°C) and Pvt-1, isolated from the Pantavat (an overnight natural fermented rice soaked with tap water at around 35°C - 37°C), produced high amount of bioethanol, 7.5% (v/v) and 6.5% (v/v), respectively at 37°C. Furthermore, a partial 26S rDNA sequencing results confirmed that the Tari-6 and Pvt-1 encoded Pichia galeiformi and Pichia guilliermondii, respectively, and later one could grow well in media containing Xylose. Our results conclude that these two yeast strains are the potential candidates for bioethanol production.

关键词： Tari Pantavat Bioethanol Thermotolerant Microorganisms Fermentation

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