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Unusual amplification in polymerase chain reaction for a plasmid containing an insert derived from bovine genomic DNA

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Natural Science 2012年第6期4卷 386-394页

作者： Satoko Isemura Akane Imai Eiichi Saitoh mamoru Isemura Department of Biochemistry School of Life Dentistry at Niigata The Nippon Dental University Niigata Japan Department of Dental Hygiene The Nippon Dental University College at Niigata Niigata Japan Graduate School of Food and Nutritional Sciences University of Shizuoka Shizuoka Japan Graduate School of Technology Niigata Institute of Technology Niigata Japan

The saliva of various animals contains praline-rich proteins which may play important roles in prevention of mineral precipitation, protection of dietary and digestive proteins from interaction with tannins, and modulation of bacterial colonization on the tooth surface. Previously, we found a segment of Escherichia coli genomic DNA in bovine tooth germ mRNA encoding the proline-rich protein P-B. To examine whether E. coli genomic DNA is present in bovine genomic DNA, we constructed a plasmid library from the bovine DNA. Although results so far have failed to indicate any such presence in the bovine nucleotides examined, experiments using the polymerase chain reaction (PCR) revealed unusual amplification of nucleotides. As an initial step of the study on possible occurrence of E. coli-derived nucleotide sequence in bovine genomic DNA of P-B, we examined the structure of the PCR products generated by unexpected amplification. The determined structure of the PCR products suggested that when the two single strand chains that grow by reading the sequence of the respective template reached a hybridizable short nucleotide structure, they became hybridized and subsequent elongation was continued by reading the sequence of the counter chain that had been elongated by reading the template. It is possible that elongation of the chain was interrupted once before the completion of amplification due to the template’s palindrome region which had formed a double strand structure during the PCR process. Such an unusual amplification made possible under certain conditions in a DNA sequence may be one of the mechanisms for the genetic recombination found in our previous study.

关键词： Bovine Genomic DNA PCR Amplification Nucleotide Sequence Palindrome

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Multiple Chaos Generator by Neural-Network-Differential-Equation for Intelligent Fish-Catching

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通讯和计算机（中英文版） 2013年第6期10卷 823-831页

作者： mamoru Minami Akira Yanou Yuya Ito Takashi Tomono Graduate School of Natural Science and Technology Okayama University Okayama 7008530 Japan

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Endoscopic ultrasound-guided gallbladder drainage for acute cholecystitis: Long-term outcomes after removal of a self-expandable metal stent

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World Journal of Gastroenterology 2017年第4期23卷 661-667页

作者： Ken Kamata mamoru Takenaka Masayuki Kitano Shunsuke Omoto Takeshi Miyata Kosuke Minaga Kentaro Yamao Hajime Imai Toshiharu Sakurai Tomohiro Watanabe Naoshi Nishida Masatoshi Kudo Department of Gastroenterology and Hepatology Kindai University Faculty of MedicineOsaka-Sayama 589-8511Japan

AIM To assess the long-term outcomes of this procedure after removal of self-expandable metal stent(SEMS). The efficacy and safety of endoscopic ultrasoundguided gallbladder drainage(EUS-GBD) with SEMS were also *** Between January 2010 and April 2015, 12 patients with acute calculous cholecystitis, who were deemed unsuitable for cholecystectomy, underwent EUSGBD with a SEMS. EUS-GBD was performed under the guidance of EUS and fluoroscopy, by puncturing the gallbladder with a needle, inserting a guidewire, dilating the puncture hole, and placing a SEMS. TheSEMS was removed and/or replaced with a 7-Fr plastic pigtail stent after cholecystitis improved. The technical and clinical success rates, adverse event rate, and recurrence rate were all *** The rates of technical success, clinical success, and adverse events were 100%, 100%, and 0%, respectively. After cholecystitis improved, the SEMS was removed without replacement in eight patients, whereas it was replaced with a 7-Fr pigtail stent in four patients. Recurrence was seen in one patient(8.3%) who did not receive a replacement pigtail stent. The median follow-up period after EUS-GBD was 304 d(78-1492).CONCLUSION EUS-GBD with a SEMS is a possible alternative treatment for acute cholecystitis. Long-term outcomes after removal of the SEMS were excellent. Removal of the SEMS at 4-wk after SEMS placement and improvement of symptoms might avoid migration of the stent and recurrence of cholecystitis due to food impaction.

关键词： Endoscopic ultrasound-guided gallbladder drainage Cholecystitis Endoscopic ultrasound-guided biliary drainage

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振动幅度对两相流局部参数变化影响研究

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核动力工程 2017年第2期38卷 38-42页

作者：肖秀朱庆子陈绍文张亚军贾海军 mamoru Ishii 清华大学核能与新能源技术研究院北京100084 普渡大学核工程系美国西拉法叶47906 国立清华大学工程与系统科学系中国台湾30013

为研究实验段振动对管内两相流局部参数变化的影响,利用电导探针技术对振动状态下局部两相流特性参数包括空泡份额、气泡直径和界面浓度进行了测量。实验首先在静态工况下进行,通过固定在实验段上方的偏心轮转动获得振动工况。实验段振... 详细信息

为研究实验段振动对管内两相流局部参数变化的影响,利用电导探针技术对振动状态下局部两相流特性参数包括空泡份额、气泡直径和界面浓度进行了测量。实验首先在静态工况下进行,通过固定在实验段上方的偏心轮转动获得振动工况。实验段振动周期保持在0.5 s,偏心轮提供的振动幅度分别为4.8 mm、9.5mm和15.8 mm。实验结果表明,振动对环管内气-水两相流局部时均参数分布影响很小。但振动引起的附加惯性力作用使两相流局部参数径向分布在实验段振动周期中发生明显变化,而且局部参数的变化幅度随实验段振幅的增加而显著增大。在含气率较低的流动工况,当振幅增大到15.9 mm时振动工况下径向空泡份额峰值较静态工况下的空泡份额峰值的增量可以达到70%。但振动对局部流动参数的影响随气流量增大而降低。

关键词：振动幅度两相流局部参数电导探针

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Response of Stretched Cylindrical Diffusion Flame to Sinusoidal Oscillation of Air Flow Velocity

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Journal of Mechanics Engineering and Automation 2017年第6期7卷 321-326页

作者： Yosuke Suenaga Hideki Yanaoka mamoru Kikuchi Shun Sasaki Department of Systems Innovation Science Iwate University Morioka 020-8551 Japan Mazda Motor Corporation Hiroshima 730-8670 Japan

An experimental study investigated the characteristics of a stretched cylindrical diffusion flame, with a convex curvature with respect to the air stream, in response to periodic air flow velocity oscillation. The fuel was methane diluted with nitrogen, and the oxidizer air. The oscillation frequency was varied from 5 to 250 Hz. The results are summarized as follows. Though the fluctuation amplitude of the air stream velocity gradient was constant with respect to the frequency, the amplitude of the fuel stream increased. The fluctuation amplitude of the flame radius changed quasi-steadily from 5 to 25 Hz, and decreased with increasing frequency in the frequency range greater than 50 Hz. The flame luminosity did not respond quasi-steadily at 5 Hz, and the oscillation amplitude of flame luminosity was less than that of a steady flame, over the same velocity fluctuation range. The oscillation amplitude of luminosity peaked at 50 Hz, and was greater than that of a steady flame. It is considered that this complex change in flame luminosity with respect to frequency was closely related to the phase difference in the respective time variations in the ratio of flame thickness to radius, the velocity gradients of the air and fuel streams, and the magnitude of these values, with the ratio of flame thickness to radius related to the flame curvature effect, the velocity gradient of the air stream correlated to the flame stretch effect, and the velocity gradient of the fuel stream impacting the fuel transportation.

关键词： Combustion diffusion flame velocity oscillation flame stretch flame curvature

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e-VLBI observations of GRB 080409 afterglow with an Australasian radio telescope network

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Research in Astronomy and Astrophysics 2016年第11期16卷 1-6页

作者： Aquib Moin Philip G. Edwards Steven J. Tingay Chris J. Phillips Anastasios K. Tzioumis Shaun W. Amy Tao An mamoru Sekido Zhong-Xiang Wang Physics Department United Arab Emirates University RO. Box 15551 A1-Ain United Arab Emirates Shanghai Astronomical Observatory Chinese Academy of Sciences Shanghai 200030 China International Centre for Radio Astronomy Research Curtin University Bentley WA Australia CSIRO Astronomy and Space Science P.O. Box 76 Epping NSW 2121 Australia Kashima Space Research Center National Institute of Information and Communications Technology (NICT) 893-1 Hirai Kashima 314-0014 Ibaraki Japan Key Laboratory of Radio Astronomy Chinese Academy of Sciences Nanjing 210008 China Osservatorio di Radio Astronomia Istituto Nazionale di Astrofisica Italy

Transcontinental e-VLBI observations were conducted in June 2008 with telescopes in Australia, China and Japan. Detections were made of the radio-loud quasar PKS B0727-115, which shows superlu- minal motion, and the intra-day variable quasar PKS B0524＋034. The latter source was used as a phase reference calibrator for observations at the position of the gamma-ray burst GRB 080409, for which an upper limit to the radio emission is set. Australia Telescope Compact Array data were also used to derive a limit on the radio flux density of the GRB afterglow. These observations demonstrate the capability to form a large Australasian radio telescope network for e-VLBI, with data transported and processed in realtime over high capacity networks. This campaign represents the first step towards more regular e-VLBI observations in this region.

关键词： techniques: interferometric -- galaxies: active -- gamma rays: bursts

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Study of the genus Oxaenanus Swinhoe(Lepidoptera:Erebidae:Herminiiae)from China,with description of a new species

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Entomotaxonomia 2021年第1期43卷 7-12页

作者： Zhipeng MIAO mamoru OWADA Min WANG Department of Entomology College of AgricultureSouth China Agricultural UniversityGuangzhouGuangdong 510642China Jiangxi Wuyuan Tea Vocational College WuyuanJiangxi 333200China National Museum of Nature and Science Amakubo 4Tsukuba 305-0005Japan

Oxaenanus scopigeralis(Moore,1867),***.&***.,the type locality:India,is revalidated from a junior synonym of ***(Walker,1858),the type locality:*** holotype,and male and female genitalia of *** are illustrated,and *** Zhang&Han,2016 from Yunnan,China is synonymized with *** species is newly recorded from Nepal.A new species,Oxaenanus hainana ***.,closely related to ***,is described from Hainan,China by the weaker and fuzzier subterminal lines of both wings,shorter and slenderer cucullus,and more complicated vesica.

关键词： Noctuoidea new synonymy macromoths taxonomy

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Immunogenicity of DNA and Recombinant Sendai Virus Vaccines Expressing the HIV-1 gag Gene

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Virologica Sinica 2008年第4期23卷 295-304页

作者： Xia FENG Shuang-qing YU Tsugumine Shu Tetsuro Matano mamoru Hasegawa Xiao-li WANG Hong-tao MA Hong-xia LI Yi ZENG State Key Laboratory for Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100052 China DNA VEC Corporation Tsukuba 305-0051 Japan Department of Microbiology Graduate School of Medicine University of Tokyo Tokyo 113-0033 Japan College of Life Science and Bioengineering Beijing University of Technology Beijing 100022 China

Combinations of DNA and recombinant-viral-vector based vaccines are promising AIDS vaccine methods because of their potential for inducing cellular immune responses. It was found that Gag-specific cytotoxic lymphocyte (CTL) responses were associated with lowering viremia in an untreated HIV-1 infected cohort. The main objectives of our studies were the construction of DNA and recombinant Sendai virus vector (rSeV) vaccines containing a gag gene from the prevalent Thailand subtype B strain in China and trying to use these vaccines for therapeutic and prophylactic vaccines. The candidate plasmid DNA vaccine pcDNA3.1(+)-gag and recombinant Sendai virus vaccine (rSeV-gag) were constructed separately. It was verified by Western blotting analysis that both DNA and rSeV-gag vaccines expressed the HIV-1 Gag protein correctly and efficiently. Balb/c mice were immunized with these two vaccines in different administration schemes. HIV-1 Gag-specific CTL responses and antibody levels were detected by intracellular cytokine staining assay and enzyme-linked immunosorbant assay (ELISA) respectively. Combined vaccines in a DNA prime/rSeV-gag boost vaccination regimen induced the strongest and most long-lasting Gag-specific CTL and antibody responses. It maintained relatively high levels even 9 weeks post immunization. This data indicated that the prime-boost regimen with DNA and rSeV-gag vaccines may offer promising HIV vaccine regimens.

关键词： HIV-1 vaccines gag gene DNA vector Sendai virus

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巴金——一个理想主义者的世纪守望

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中国现代文学研究丛刊 2006年第2期 123-129页

作者：山口守日本大学文理学部中文科

—个作家究竟应当是什么时候才算“死了”呢？是生命枯竭的自然死亡呢，还是作为文学生产者的“死亡”？抑或是其文学声誉的“死亡”？恐怕有人会认为作家是死于生命尽管依然持续却已写不出作品那个时刻；也有可能生命与文学生产两者都... 详细信息

—个作家究竟应当是什么时候才算“死了”呢？是生命枯竭的自然死亡呢，还是作为文学生产者的“死亡”？抑或是其文学声誉的“死亡”？恐怕有人会认为作家是死于生命尽管依然持续却已写不出作品那个时刻；也有可能生命与文学生产两者都在持续却已为社会所忘却而迎来了“死亡”；

关键词：理想主义者守望世纪巴金 “死亡” 文学生产自然死亡生命生产者作家

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巴金与西班牙内战

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中国现代文学研究丛刊 2007年第1期 46-61页

作者：山口守日本大学文理学部

在30年代“国防文学”论战中,由于巴金受到共产党方面意识形态理论家们的攻击,使得西班牙内战及统一战线问题与抗日战争及统一战线问题交互重合的认识得以普及,在这样的语境中结果反而导致巴金的思想受到轻视。自论战以来虽然经历了将... 详细信息

在30年代“国防文学”论战中,由于巴金受到共产党方面意识形态理论家们的攻击,使得西班牙内战及统一战线问题与抗日战争及统一战线问题交互重合的认识得以普及,在这样的语境中结果反而导致巴金的思想受到轻视。自论战以来虽然经历了将近70年的时光,而仅仅只从意识形态和抗日战争历史叙述这两大要素出发来思考这一问题的倾向却依然故我。本文拟从遭受攻击的巴金一方出发,通过对安那其主义思想及国际主义的思考,向这个问题投去别种探索的光束。

关键词：西班牙巴金内战意识形态统一战线抗日战争国际主义 30年代

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