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Anti-Inflammatory and Antioxidant Properties of the Aqueous Extracts of the Leaves of Opilia amentacea (Opiliaceae)

Pharmacology & Pharmacy 2023年第9期14卷 329-347页

作者： Rainatou Boly Abdoul Gilchrist Laurent Boly Wendkouni Leila Marie Esther Belem-Kabré Kadiatou Tata traoré Boukaré Kaboré Ollo Youl Latifatou Sawadogo Mathieu Nitièma Moumouni Koala Noufou Ouédraogo Estella Noëla Hoho Youl maminata traoré-coulibaly Laboratoire de Recherche-Développement/Phytomédicaments et Médicaments (LR-D/PM) Institut de Recherche en Sciences de la Santé (IRSS) Centre National de la Recherche Scientifique et Technologique (CNRST) Ouagadougou Burkina Faso Ecole Doctorale Sciences et Technologie Laboratoire de Chimie Organique et Physique Appliquée (LCOPA) Université Joseph Ki-Zerbo Ouagadougou Burkina Faso Ecole Doctorale Sciences et Techniques Laboratoire de Biochimie et Chimie Appliquées (LABIOCA) Université Joseph Ki-Zerbo Ouagadougou Burkina Faso Ecole Doctorale Sciences de la Santé Laboratoire de Développement du Médicament (LADME) Centre de Formation de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM) Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Opilia amentacea (Opiliaceae) is a woody plant with multiple medicinal claimed effects. The present study aimed to assess the anti-inflammatory and antioxidant activities of the decoction and macerate extracts from the leaves of Opilia amentacea. Moreover, acute toxicity and phytochemical analysis were performed. The acute toxicity was evaluated on NMRI mice at 2000 mg/kg bw. The anti-inflammatory activity was studied using the carrageenan-induced mouse paw edema and the lipoxygenase inhibition assay. The radical scavenging (DPPH and ABTS), ferric-reducing antioxidant power (FRAP), and lipid peroxidation (LPO) assays were used to measure the antioxidant capacity of the extracts. Qualitative and quantitative methods served for identifying and quantifying the extract’s phytoconstituents. The decoction demonstrated low acute toxicity;the lethal dose was therefore estimated to be superior to 2000 mg/kg bw. The extracts significantly reduced the mouse paw’s thickness at 600 mg/kg bw. The extracts developed weak radical scavenging and lipid peroxidation inhibitory effects. However, the macerate showed a high ability (664.90 ± 0.71 mol Ascorbic Acid Equivalent/g dry extract) to reduce the ferric ions. Saponins, sterols, triterpenes, and flavonoids were qualitatively detected in the two extracts. Total phenolics (TP) and total flavonoids (TF) were found abundant in the extracts, especially the decoction (TP content (TPC) = 94.03 ± 2.66 mg GAE/g;TF content (TFC) = 35.05 ± 0.32 mg QE/g). Strong positive correlations existed between ferric-reducing capacity and TPC (r = 0.959) for the macerate, while TFC was mainly involved in the DPPH radical scavenging of the two extracts. Instead, most correlations were negative between the polyphenol compounds and the anti-inflammatory assays. The results indicate potent in vivo anti-inflammatory and in vitro antioxidant effects of the aqueous extracts from the leaves of Opilia amentacea. Further studies are needed to find the anti-inflamm

关键词： Opilia amentacea Aqueous Extracts Acute Toxicity Anti-Inflammatory Antioxidant

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Phytochemical Analysis and Antimicrobial Activity of Lawsonia inermis Leaf Extracts from Burkina Faso

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American Journal of Plant Sciences 2024年第7期15卷 552-576页

作者： Ollo Youl Soumaïla Konaté Ernest N. Sombié Rainatou Boly Boukaré Kaboré Moumouni Koala Arouna Zoungrana Saybou Savadogo Christian Marc Tahita Innocent Valea Halidou Tinto Adama Hilou maminata traoré-coulibaly Laboratoire de Biochimie et de Chimie Applique (LABIOCA) Universit de Ouagadougou Ouagadougou Burkina Faso Agence Nationale Pour la Scurit Sanitaire de lEnvironnement de lAlimentation du Travail et des Produits de Sant (ANSSEAT) Ouagadougou Burkina Faso Laboratoire de Recherche-Dveloppement de Phytomdicaments et Mdicaments (L-RD/PM) Institut de Recherche en Sciences de la Sant (IRSS-CNRST) Ouagadougou Burkina Faso Laboratoire de Chimie Organique et de Physique Applique (LCOPA) cole Doctorale Sciences et Technologie Universit Joseph KI-ZERBO Ouagadougou Burkina Faso Laboratoire Sciences de la Vie et de la Terre (LASVT) cole Doctorale Sciences et Technologie Universit Norbert ZONGO Koudougou Burkina Faso Unit de Recherche Clinique de Nanoro Institut de Recherche en Sciences de la Sant (IRSS-CNRST) Nanoro Burkina Faso

Lawsonia inermis is a hairless plant growing in various regions of North Africa, the Indian subcontinent, and the Middle East. It possesses many medicinal attributes, including curative properties against infectious dermatoses. This study was carried out to evaluate the phytochemical profile of the crude ethanolic extract of the plant leaves and its fractions as well as their antimicrobial activities. The phytochemical profile was performed using high-performance thin-layer chromatography (HPTLC), gas chromatography-mass spectrometry (GC-MS), and high-performance liquid chromatography (HPLC). Additionally, the phenolic and flavonoid contents were determined using the Folin-Ciocalteu spectrophotometric and the aluminum trichloride methods. Antimicrobial activity was tested using disc diffusion and microdilution methods. The presence of flavonoids, tannins, sterols, and triterpenes was revealed. GC-MS detected twelve compounds main compounds consisting of saturated and unsaturated fatty acids and phenolic and terpenoid compounds among twenty-seven components. HPLC also detected high contents of phenolic acids and flavonoids. The most abundant triterpene and sterols were ursolic acid (around 43.14 g/100g DW, 13.9 g/100g dry weight (DW), and 0.68 g/100g DW) in the crude ethanolic extract of leaves (FeLi), hexane fraction (FHLi) and dichloromethane fraction (FDLi), respectively and, β-sitosterol in FeLi (56.7 mg/100g DW), FHLi (10.55 g/100g DW), FDLi (106.1 mg/100g DW) and butanol fraction (FBLi) (357.4 mg/100g DW). Among the flavonoids, rutin = 3.24 g/100g and quercetin = 0.63 g/100g in the ethanolic extract, rutin = 15.73 g/100g in the dichloromethane fraction, and rutin = 0.23 g/100g) in the aqueous fraction;and among phenolic compounds, caffeic acid (37.65 g/100g DW) and vanillic acid (22.70 g/100g DW) were the most important in the ethyl acetate fraction (FAeLi). All organic fractions exhibited interesting antibacterial and antifungal activities against the tested s

关键词： Lawsonia inermis Phytochemical Profile HPLC GC-MS Analysis Antimicrobial Activity

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Anti-Inflammatory and Antioxidant Properties of the Aqueous Extracts of the Leaves of Opilia amentacea (Opiliaceae)

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Pharmacology & Pharmacy（药理与制药） 2023年第9期

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Phytochemical Analysis and Antimicrobial Activity of Lawsonia inermis Leaf Extracts from Burkina Faso

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American Journal of Plant Sciences（美国植物学期刊） 2024年第7期 552页

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Characterization of Seed Storage Proteins in Eight Bambara Groundnut Landraces in Burkina Faso

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Agricultural Sciences 2023年第9期14卷 1268-1276页

作者： Assita traoré-Barro Emmanuel A. M. Thiombiano Karidiatou Gnankambary Mounyratou Rabo Orokia coulibaly Salimata traoré Oumar traoré Laboratoire National de Biosécurité Agence Nationale de Biosécurité Ouagadougou Burkina Faso Laboratoire de Biochimie et de Chimie Appliquée Université Joseph Ki-Zerbo Ouagadougou Burkina Faso Laboratoire de Génétique et amélioration des plantes (LAGAMEP) Institut de l’Environnement et Recherches Agricoles Ouagadougou Burkina Faso Laboratoire de protection et défense des cultures (LAPRODEC) Institut de l’Environnement et Recherches Agricoles Ouagadougou Burkina Faso

The Bambara groundnut Vigna subterranea (L.) Verdc. is a drought-resistant indigenous African grain legume with significant nutritional and agronomic potential. This study aimed to characterize the seed storage proteins of eight Bambara groundnut landraces. Seeds of Bambara groundnut landraces were collected from local markets in Burkina Faso, and total soluble protein as well as protein fractions were extracted. Crude protein content was determined by the Kjeldahl method, and soluble proteins were quantified using Bradford dye binding assay. The average crude protein content of the seeds was found to be 18.46%, with variations ranging from 17.69% to 19.17% among the different landraces. Most of the protein content was soluble, constituting approximately 87.04% of the total crude protein. Albumin fraction was the most dominant, representing about 95.42% of the total soluble proteins. The globulin, prolamin and glutelin fractions accounted for 1.82%, 0.13% and 1.17% of the soluble proteins, respectively. The findings provide valuable insights into the protein composition of Bambara groundnut landraces and contribute to our understanding of its nutritional potential, laying the groundwork for further research on crop improvement and sustainable agriculture practices.

关键词： Bambara Groundnut Landraces Protein Protein Fractions

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Soil Carbon Stocks Evolution in Organic Cotton-Based Cropping Systems in Southern and Northern Sudanese Agro-Ecological Zones of Burkina Faso

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Journal of Agricultural Science and Technology(B) 2019年第6期9卷 392-402页

作者： Mamadou traoré Bazoumana Koulibaly Kalifa coulibaly Issouf traoré Moussa Gnissien Tahibou Paré Hassan Bismarck Nacro Institut du Développement Rural(IDR) Laboratoire d´Etude et de Recherche sur la Fertilitédes sols(LERF)UniversitéNazi Boni de Bobo Dioulasso(UNB)01 BP1091 Bobo-Dioulasso 01Burkina Faso Institut de l’Environnement et de Recherches Agricoles(INERA) Programme Coton Burkina Faso.01 BP 208 Bobo Dioulasso 01 Bureau National des Sols(BUNASOLS) 03 BP 7005Secteur 27 OuagadougouBurkina Faso

Variation of soil carbon stock in the cropping systems is an important indicator of their *** present study was conducted in 2015 and 2018 in seven organic cotton production areas distributed over the Southern and Northern Sudan agro-ecological zones in Burkina *** samples were collected in 2015 as baseline and in 2018,after three years of cropping seasons,to determine the variations in carbon stocks in plots under organic farming *** were also conducted to understand the fertilization practices implemented by producers during the same *** results revealed that the recommended fertilization packages were not respected due to low production capacity and under using of organic *** three years of cropping in 2018,the deficit of organic restitution has led to a high decline of the soil carbon *** decline was more severe in the 0-10 cm depth in some soils where the decrease in carbon stocks ranged from-4.6 t/ha to-5.5 t/*** correlation between soil types and their carbon stocks in the 0-10 cm soil layer was found to be significant(p<0.05)in the Northern Sudan agro-ecological zone with adjusted R2=74%and 54%in 2015 and 2018,respectively,and adjusted R2=56%(2015)and 44%(2018)in the Southern Sudan agro-ecological *** three years of organic cotton-based farming,a decrease in the correlation between soil types and their carbon content was observed in the majority of *** results show that the process of carbon storage in soil is more influenced by agricultural practices and agro-ecological conditions than by the soil type.

关键词： Organic cotton soil carbon stocks cropping system Burkina Faso

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Management of Spinal Injuries Secondary to Rockfall in Traditional Mines

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Surgical Science 2023年第2期14卷 104-110页

作者： coulibaly Kalifa traoré Youssouf Tambassi Sory Ibrahim Sanogo Cheick Oumar Diallo Aboubacar traoré Soumana traoré Issa Djerma Issa Orthopedic Trauma Department of Kati University Hospital Koulikoro Mali Neurosurgery Department of the University Hospital of Kati Koulikoro Mali

Introduction: Spinal injuries represent the whole of the mechanisms responsible for vertebral, disco-ligamentary and spinal cord injuries. Spinal cord injury is life-threatening and is responsible for functional consequences that make the subsequent socio-economic integration of victims difficult. In developing countries, spinal injuries in traditional gold mining are becoming more and more frequent. Purpose: The purpose of this study was to describe the anatomopathological aspects and the results of surgical treatment of spinal injuries secondary to traditional mine collapses at the University Hospital of Kati. Patients and Methods: This was a prospective, descriptive study over 18 months, from November 2017 to May 2019. Included in this study were patients operated on for spinal trauma secondary to rockfall in traditional mines. The neurological status was assessed in each patient since admission to the department using the Fränkel score. Decompression by laminectomy combined with titanium device placement was preferred in lumbar, thoracic and thoraco-lumbar surgery. We performed an anterior cervical spine approach with SENEGAS plate placement in cervical injuries. Motor physiotherapy was associated whenever recovery was not complete. At a minimum follow-up of 6 months, all our patients were re-evaluated by the Fränkel score. Results: The mean age was 31 years with extremes of 10 and 49 years. The majority of patients were men, 95%. Twenty patients underwent surgery. According to the spinal segments, the cervical spine was affected in 05 cases, the thoracic spine in 08 cases and the lumbar spine in 17 cases. The neurological examination revealed tetraplegia (n = 5), paraplegia (n = 12) and paraparesis (n = 3). According to the types of lesions, we collected 12 cases of fractures, 4 cases of fracture-luxations and 04 cases of pure luxations. According to the site, the fracture was uni-articular (n = 3), corporal (n = 15) and transverse apophyseal (n = 1). A

关键词： Trauma Spine Traditional Gold Mining Mali

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Sacrospinous Fixation in the Gynecology Department of Hôpital du Mali

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Open Journal of Obstetrics and Gynecology 2021年第1期11卷 20-26页

作者： traoré Alassane Sissoko Abdoulaye coulibaly Mamadou Bakary traoré Soumana Oumar Sima Mahamadou Bocoum Amadou traoré Soumaila Touré Moustapha Tegueté Ibrahima traoré Youssouf Obstetrics and Gynecology Department at Hopital du Mali Bamako Mali Obstetrics and Gynecology Department at the Teaching Hospital Mère-Enfant Bamako Luxembourg Obstetrics and Gynecology Department at the Referral Health Center Commune V Bamako Mali Obstetrics and Gynecology Department at the Teaching Hospital Point G Bamako Mali Obstetrics and Gynecology Department at the Teaching Hospital Gabriel Touré Bamako Mali Obstetrics and Gynecology Department at the Regional Hospital of Sikasso Sikasso Mali

Sacrospinous fixation (SSF) or Richter’s intervention (RI) aims to treat genital prolapse by securing the posterior vaginal wall to the small sacrospinous ligament. It is performed by low approach and includes a dissection of the pararectal space, visual exposure of the sacrospinous ligament and a needle with strait needle holder with nonabsorbable threads. It is often associated with a more complex corrective procedure, including cystocele cure, vaginal hysterectomy and posterior myorrhaphy. The objective of this study is to report the results of SSF in the gynecology department of Hopital du Mali. A descriptive study was conducted from September 2014 to September 2015 concerning 37 patients operated on for uterine prolapse (UP). All patients with grade III UP were included in our study in whom a unilateral hysterectomy (UH) and sacrospinous fixation (SSF) were performed. All the patients were scheduled. Preoperatively they had benefited from an assessment and a pre-anesthetic consultation. Hospitalization of at least 24 hours prior to the operation was required. Postoperative follow-up was two years with a physical examination at 3 months, 9 months and 15 months, and phone calls between physical consultations. During the study period, we performed 37 RIs. The mean age of the patients was 48 years with extremes of 41 to 73 years. The large multiparity was found out in 35 cases (94.59%), the pauciparous were two with 3 deliveries for each. Long labor of more than 18 hours was found out in 9 patients (24.32%) and home delivery in 13 cases (35.13%). The duration of the occurrence of prolapse was at least two years and 35 patients were going through menopause. The type of anesthesia used for the surgery was spinal anesthesia for all patients. The average duration of the operation was 90 minutes. Complications occurred in three patients or 8.10% of cases, two cases of acute urine retention and one case of hematoma of the para-rectal space. The medium time of hospital st

关键词： Sacrospinous Fixation Genital Prolapse Hôpital du Mali

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Protein Profiles of Pod Borer Maruca Resistant Transgenic Cowpea

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American Journal of Plant Sciences 2023年第12期14卷 1453-1463页

作者： Mounyratou Rabo Teyioue Benoit Joseph Batieno Assita traoré-Barro Salimata traoré Orokia coulibaly Aboubacar Toguyeni Chantal Kaboré-Zoungrana Oumar traoré Laboratoire National de Biosécurité Agence Nationale de Biosécurité (ANB) Ouagadougou Burkina Faso Institut de l’Environnement et Recherches Agricoles Département Productions Végétales Ouagadougou Burkina Faso Laboratoire d’Etudes et de Recherches sur les Ressources Naturelles et les Sciences de l’Environnement (LERNSE) Université Nazi BONI Bobo-Dioulasso Burkina Faso Laboratoire de Génétique et amélioration des plantes (LAGAMEP) Institut de l’Environnement et Recherches Agricoles Ouagadougou Burkina Faso Laboratoire de protection et défense des cultures (LAPRODEC) Institut de l’Environnement et Recherches Agricoles Ouagadougou Burkina Faso

The grain legume cowpea Vigna unguiculata (L.) Walp. is a major protein source used for food and feed in Sub-Saharan Africa. The crop is affected by the pod borer Maruca vitrata against which transgenic lines were developed as part of the genetic control approach. This study aimed to assess the protein profiles in seeds and leaves of transgenic cowpea lines and their non-transgenic near-isogenic counterparts. Crude protein content was determined by the Kjeldahl method, and soluble proteins were quantified using Bradford dye binding assay. The average crude protein content ranged between 21.61% and 26.58% in the seeds and between 10.86% and 17.90% in the leaves. Total solubility varied between 13.03% and 20.64%. Osborne’s protein fractions contents in the seeds were 52.41% - 69.52% (albumin), 4.62% - 7.19% (globulin), 7.95% - 11.40% (glutelin) and 3% - 4% (prolamin). In any case, protein content differed significantly between cowpea genotypes but not between pairs of transgenic/non-transgenic lines. Insecticidal Cry1Ab protein expressed by transgenic lines was only detected in the albumin and globulin fractions. Altogether, these findings enhance our understanding of the effects of genetic modification on cowpea protein content and composition, with potential implications for nutritional and safety assessments.

关键词： Cowpea Protein Cry1Ab Protein Fractions

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Kati Chu BSS External Genital Organ Delivery Syndrome: Inguinal Pedicle Flap Plasty

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Surgical Science 2021年第11期12卷 357-364页

作者： Koniba Keita Sidiki Keita Oulematou coulibaly Abdoulaye Diarra Fodé Mory Keita Boubacar Kone Lassina traoré Salia coulibaly Amadou traoré Assitan Kone Amadou Kassogué Issa traoré Oumou traoré Ismael Konaré Daouda Diallo Drissa traoré Bacary T. Dembélé General Surgery Department Hospital BSS Kati Mali General Surgery “A” Department Hospital Point-G Bamako Mali Reference Health Centre of the Commune VI Bamako Mali Medical Imaging Department Hospital BSS Kati Mali General Surgery Department Hospital Gabriel Toure Bamako Mali Urology Department Hospital BSS Kati Mali Anesthesia Resuscitation Department Hospital BSS Kati Mali General Surgery “B” Department Hospital Point-G Bamako Mali

Fournier’s syndrome is a poly microbial necrotizing fasciitis, of severe evolution of the perineum and external genitals. Objectives: We report the experience of the service in the management of Fournier gangrene of the external genital organs by inguinal flap pediculated in front of the loss of significant substances of the scrotum. Patients and method: This was a mono-centric retrospective study from January 2008 to August 2021 in the General Surgery Department of the Bocar Sidi Sall University Hospital (CHU BSS) in Kati. It focused on patients treated for Fournier gangrene of external genitals. The variables studied were age, sex, blood count, germs encountered, the antibiogram, duration of evolution, reconstruction of the scrotum by pedicle flap, and morbidity. Results: During the study period we collected 5 cases of Fournier gangrene of external genitals in the service. The average age was 57.6 years and all the patients were male. The average duration of evolution was 5 days for an average age of 57.6 years. All patients were male. Echerichia coli (E. coli)

关键词： Fournier’s Gangrene Scrotum Inguinal Pedicular Flap

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