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A novel luciferase immunosorbent assay performs better than a commercial enzyme-linked immunosorbent assay to detect MERS-CoV specific IgG in humans and animals

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Biosafety and Health 2019年第3期1卷 134-143页

作者： Wenling Wang Tianyu Wang Yao Deng Peihua niu Ruhan A Jincun Zhao malik Peiris Shixing Tang Wenjie Tan MHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChina CDCBeijing 102206China Guangdong Provincial Key Laboratory of Tropical Disease Research Department of EpidemiologySchool of Public HealthSouthern Medical UniversityGuangzhou 510515China State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory DiseaseThe first Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510182China School of Public Health Li Ka Shing Faculty of MedicineThe University of Hong KongPokfulamHong Kong Special Administrative Region Center for Biosafety Mega-science Chinese Academy of SciencesBeijing 100101China

The Middle East respiratory syndrome(MERS)is a lethal zoonosis caused by MERS coronavirus(MERS-CoV)and poses a significant threat to public health ***,a rapid,sensitive,and specific serologic test for detecting anti-MERS-CoV antibodies in both humans and animals is urgently needed for the successful management of this ***,we evaluated various novel luciferase immunosorbent assays(LISA)based on nucleocapsid protein(nP)as well as fragments derived from spike protein(S)including subunit 1(S1),n terminal domain(nTD),receptorbinding domain(RBD)and subunit 2(S2)of S for the detection of MERS-CoV-specific *** proteins,including nanoluciferase(nLuc)and various fragments derived from the nP or S protein of MERS-CoV,were expressed in human embryonic kidney 293 T *** that detected anti-MERS-CoV IgG were further developed using cell lysates expressing various fusion *** of human or animal samples infected with MERS-CoV were used to analyze the sensitivity and specificity of various LISAs in reference to a MERS-CoV RT-PCR,commercial S1-based ELISA,and pseudovirus particle neutralization test(ppnT).Our results showed that the S1-,RBD-,and nP-LISAs were more sensitive than the nTD-and S2-LISAs for the detection of anti-MERS-CoV ***,the S1-,RBD-,and nP-LISAs were more sensitive(by at least 16-fold)than the commercially available ***,the S1-,RBD-,and nPLISA specifically recognized anti-MERS-CoV IgG and did not cross-react with samples derived from other human CoV(OC43,229E,HKU1,nL63)-infected *** importantly,these LISAs proved their applicability and reliability for detecting anti-MERS-CoV IgG in samples from camels,monkeys,and mice,among which the RBD-LISA exhibited excellent *** results of this study suggest that the novel MERS-CoV RBD-and S1-LISAs are highly effective platforms for the rapid and sensitive detection of anti-MERS-CoV IgG in human and animal *** assays have

关键词： Luciferase immunosorbent assay(LISA) MERS-CoV Serological IgG detection Samples of humans and animals

A comparative study of conventional and supercritical fluid extraction methods for the recovery of secondary metabolites from Syzygium campanulatum Korth

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第9期17卷 683-691页

作者： Abdul Hakeem MEMOn Mohammad Shahrul Ridzuan HAMIL Madeeha LAGHARI Fahim RITHWAn Salman ZHARI Mohammed Ali Ahmed SAEED Zhari ISMAIL Amin malik Shah Abdul MAJID Department of Pharmaceutical Chemistry School of Pharmaceutical Sciences Universiti Sains Malaysia 11800 Minden Penang Malaysia Center of Lipids Engineering and Applied Research (CLEAR) lbnu Sina Institute for Scientific & Industrial Research (lbnu Sina ISIR) Universiti Teknologi Malaysia 81310 Johor Bahru Johor Malaysia EMAn Research and Testing Laboratory School of Pharmaceutical Sciences Universiti Sains Malaysia 11800 Minden Penang Malaysia

Syzygium campanulatum Korth is a plant, which is a rich source of secondary metabolites （especially flavanones, chalcone, and triterpenoids）. In our present study, three conventional solvent extraction （CSE） techniques and supercritical fluid extraction （SFE） techniques were performed to achieve a maximum recovery of two flavanones, chalcone, and two triterpenoids from S. campanulatum leaves. Furthermore, a Box-Behnken design was constructed for the SFE technique using pressure, temperature, and particle size as independent variables, and yields of crude extract, individual and total secondary metabolites as the dependent variables. In the CSE procedure, twenty extracts were produced using ten different solvents and three techniques （maceration, soxhletion, and reflux）. An enriched extract of five secondary metabolites was collected using n-hexane：methanol （1：1） soxhletion. Using food-grade ethanol as a modifier, the SFE methods produced a higher recovery （25.5%-84.9%） of selected secondary metabo- lites as compared to the CSE techniques （0.92%-66.00%）.

关键词： Syzygium campanulatum Supercritical fluid extraction Conventional solvent extractions Flavanones Chalcone Triterpenoids

HG3In2TE6- A nEW MATERIAL FOR DETECTORS FOR 1.3-1.55 μM SPECTRAL RAnGE

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HG3IN2TE6- A NEW MATERIAL FOR DETECTORS FOR 1.3-1.55 μM SPE...

颈动脉斑块性质、狭窄和内膜中层厚度与年龄、性别的关系

第8届国际材联电子材料国际研讨会

作者： Peter n. Gorley Olena G. Grushka Zinaida M. Grushka Alexander I. malik Yuri V.Vorobiev Jesus González-Hernández Paul P. Horley Chemivtsi national University Department of Physical Electronics 2 Kotsyubynsky str. 58012 Chernivtsi Ukraine InAOE Electronics Department Apdo.51 Puebla Pue. 72000 Mexico CInVESTAV-Querétaro Libramiento norponiente 2000 Fracc. Real de Juriquilla 76230 Querétaro México Chernivtsi national University Department of Physical Electronics 2 Kotsyubynsky str. 58012 Chernivtsi Ukraine CINVESTAV-Querétaro Libramiento Norponiente 2000 Fracc. Real de Juriquilla 76230 Querétaro México

Modern fiber optics for the spectral ranges of 1.33-1.55μm makes use of Ge photodiodes and AB compound-based ones as detectors. The disadvantage of the first material mentioned is low quantum yield;the second one is expensive and still has difficulties to obtain big samples with the necessary photoelectric *** propose to use HgInTe compound (MIT) for optimized high-quality detectors for the given spectral ranges. This compound is cheap and has interesting physical properties. The congruent character of

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中华高血压杂志 2016年第1期24卷 3-3页

作者：刘莉叶鹏 Boulos nM Gardin J malik S Postley J Wong n

颈动脉内膜中层厚度（carotid intima-media thickness,CIMT）是一项公认的心血管事件的预测指标,然而,CIMT、年龄和性别的不同对斑块发生率和狭窄严重程度的影响的研究资料较缺乏。研究人员利用一项大型CIMT筛查注册研究的数据,评估不... 详细信息

颈动脉内膜中层厚度（carotid intima-media thickness,CIMT）是一项公认的心血管事件的预测指标,然而,CIMT、年龄和性别的不同对斑块发生率和狭窄严重程度的影响的研究资料较缺乏。研究人员利用一项大型CIMT筛查注册研究的数据,评估不同CIMT水平、年龄和性别对颈动脉斑块形成和狭窄严重程度的影响。

关键词：颈动脉斑块内膜中层厚度 CIMT carotid 心血管事件颈动脉超声 intima 斑块发生率研究人群软斑块

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豆腐中库特氏菌生长动力学模型和货架期预测

农业工程学报 2009年第S1期25卷 82-86页

作者：李除夕董明盛陈晓红姜梅吕欣努尔古丽.热合曼 malik Muhammad Hashim 南京农业大学食品科技学院南京210095 新疆师范大学生命科学与化学学院乌鲁木齐830054 Food Technology Section Agricultural Research InstituteDera Ismail Khan

以引起豆腐腐败的特定腐败菌库特氏菌为研究对象,研究不同温度对其生长的影响,定量评价温度对豆腐货架期的影响,为货架期快速有效的估测提供有效的手段。将库特氏菌接种到豆腐表面,在4、12、20、30℃条件下贮藏,由此建立一级和二级生长... 详细信息

以引起豆腐腐败的特定腐败菌库特氏菌为研究对象,研究不同温度对其生长的影响,定量评价温度对豆腐货架期的影响,为货架期快速有效的估测提供有效的手段。将库特氏菌接种到豆腐表面,在4、12、20、30℃条件下贮藏,由此建立一级和二级生长动力学模型以及剩余货架期预测模型。结果表明,Gompertz函数能够很好的描述豆腐中的微生物生长动态,建立了4种温度下豆腐的微生物生长动力学模型。采用平方根模型(Belehradek)描述温度对最大比生长速率(μmax)和延滞时间(Lag)的影响,结果表明呈现良好的线性关系。用豆腐在8℃和25℃的库特氏菌实测值进行验证,偏差度(Bf)和准确度(Af)分别为1.04、1.02和1.20、1.18。获得的剩余货架期的预测模型为:SL=Lag-[(8.6-n0)/(μmax·2.718)]·{ln[-ln(7.14-n0)/(8.6–n0)]-1},用豆腐贮藏在8℃和25℃的货架期实测值验证建立的模型,预测值和实测值的相对误差分别为-3.47%和4.89%,说明建立的模型能够快速可靠的预测豆腐的微生物学品质和剩余货架期。

关键词：库特氏菌豆腐生长动力学模型货架期预测

反钙钛矿化合物Mn_3Co_(1-x)n的磁相变及其反常热膨胀性能研究

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中国科技论文 2016年第11期11卷 1219-1222页

作者：史可文孙莹邓司浩胡鹏伟王蕾卢会清 MUHAMMAD Imran malik 赵文君王聪北京航空航天大学物理科学与核能工程学院北京100191

为了研究Co空位对Mn_3Con化合物磁性质和热膨胀性能的影响,采用固相烧结法制备了名义配比为Mn_3Co_(1-x)n(x=0,0.1,0.2,0.3)的反钙钛矿结构化合物,并研究了该类化合物的晶格、磁转变和热膨胀性能。结果表明:所有的样品均显示出2个磁转变... 详细信息

为了研究Co空位对Mn_3Con化合物磁性质和热膨胀性能的影响,采用固相烧结法制备了名义配比为Mn_3Co_(1-x)n(x=0,0.1,0.2,0.3)的反钙钛矿结构化合物,并研究了该类化合物的晶格、磁转变和热膨胀性能。结果表明:所有的样品均显示出2个磁转变点;随着x的增加,低温磁转变点往高温移动,高温磁转变点往低温移动;同时,所有的样品中都发现了反常热膨胀行为,包括低于270K的低热膨胀和270K附近的负热膨胀;低热膨胀区间的线膨胀系数随着x的增加而呈现出减小的趋势;值得注意的是,当x=0.3时,样品在100~240 K温度区间内显示出近零热膨胀行为,其热膨胀系数α=9.70×10^(-7) K^(-1);Mn_3Co_(1-x)n化合物中的反常热膨胀行为可能是磁体积效应所致。

关键词：反钙钛矿化合物 Mn3Co1-xn 磁转变热膨胀

施氮量及其施用时间对甘蔗生长、产量和质量的影响

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甘蔗糖业 1982年第9期 40-42+39页

作者： S.S.narwal D.S.malik 黄勇

甘蔗是生长期长的高产作物,要从土壤中吸取大量养分。Kirtikar和Bajpai（1972）曾报道,甘蔗产量达到每公顷53.0吨时,每公顷分别吸取n、P;O;, K;O、CaO、MgO90.7、42.3、185.3, 77.44和58.6公斤。Ray等（1975）发现甘蔗产量达到每公顷63.5吨

关键词：甘蔗糖料作物施氮量施用时间茎径产量

Utility of liver biopsy in predicting clinical outcomes after percutaneous angioplasty for hepatic venous obstruction in liver transplant patients

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World Journal of Hepatology 2015年第14期7卷 1884-1893页

作者： Ammar Sarwar Edward Ahn Ian Brennan Olga R Brook Salomao Faintuch Raza malik Khalid Khwaja Muneeb Ahmed Divison of Vascular and Interventional Radiology Beth Israel Deaconess Medical Center/Harvard Medical School Division of Gastroenterology and Hepatology Beth Israel Deaconess Medical Center/Harvard Medical School Division of Transplant Surgery Beth Israel Deaconess Medical Center/Harvard Medical School

AIM: To determine utility of transplant liver biopsy in evaluating efficacy of percutaneous transluminal angioplasty(PTA) for hepatic venous obstruction(HVOO). METHODS: Adult liver transplant patients treated with PTA for HVOO(2003-2013) at a single institution were reviewed for pre/post-PTA imaging findings, manometry(gradient with right atrium), presence of HVOO on prePTA and post-PTA early and late biopsy(EB and LB, 60 d after PTA), and clinical outcome, defined as good(no clinical issues, non-HVOO-related death) or poor(surgical correction, recurrent HVOO, or HVOOrelated death). RESULTS: Fifteen patients meeting inclusion criteria underwent 21 PTA, 658 ± 1293 d after *** procedures with pre-PTA biopsy(n = 19), no difference was seen between pre-PTA gradient in 13/19 procedures with HVOO on biopsy and 6/19 procedures without HVOO(8 ± 2.4 mm Hg vs 6.8 ± 4.3 mm Hg; P = 0.35). Post-PTA, 10/21 livers had EB(29 ± 21 d) and 9/21 livers had LB(153 ± 81 d). On clinical follow-up(392 ± 773 d), HVOO on LB resulted in poor outcomes and absence of HVOO on LB resulted good outcomes. Patients with HVOO on EB(3/7 good, 4/7 poor) and no HVOO on EB(2/3 good, 1/3 poor) had mixed outcomes. COnCLUSIOn: negative liver biopsy greater than 60 d after PTA accurately identifies patients with good clinical outcomes.

关键词： Hepatic venous outflow obstruction Livertransplantation Post-transplant biopsy Angioplasty

Integrated silicon ohotonic MEMS

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Microsystems & nanoengineering 2023年第2期9卷 227-248页

作者： niels Quack Alain Yuji Takabayashi Hamed Sttari Pieirre Edinger Gaehun Jo Simon JBleker Carlos Errando-Herranz Kristinn BGylfason Frank niklaus Umar khan Peter Verheyen Arun Kumar malik Jun Su Lee Moises Jezzini Padraic Morrissey Cleitus Antony Peter O'Brien Wim Bogaertso Ecole Polytechnique Federale de Lausanne(EPFL) 1015 LausanneSwitzerland school of Aerospace Mechanical and Mechatronic EngineeringThe University of SydneyCamperdownNSW 2006Australia swiss Center for Electronics and Microtechnology(CSEM) 2002 NeuchatelSwitzerland Division of Micro and nanosystems KTH Royal Institute of Technology11428 StockholmSweden Department of Information Technology Photonics Research GroupGhent University-IMECTechnologiepark-Zwijnaarde 1269052 GentBelgium imec vzw.3DSIP Department Si Photonics GroupKapeldreef 753001 LeuvenBelgium Tyndall national Institute Lee Maltings Complex Dyke ParadeCork T12 R5CPIreland

Silicon photonics has emerged as a mature technology that is expected to play a key role in critical emerging applications,including very high data rate optical communications,distance sensing for autonomous vehicles,photonic-accelerated computing,and quantum information *** success of silicon photonics has been enabled by the unique combination of performance,high yield,and high-volume capacity that can only be achieved by standardizing manufacturing ***,standardized silicon photonics technology platforms implemented by foundries provide access to optimized library components,including low-loss optical routing,fast modulation,continuous tuning,high-speed germanium photodiodes,and high-effciency optical and electrical ***,silicon's relatively weak electro-optic effects result in modulators with a significant footprint and thermo-optic tuning devices that require high power consumption,which are substantial impediments for very large-scale integration in silicon *** systems(MEMS)technology can enhance silicon photonics with building blocks that are compact,low-loss,broadband,fast and require very low power ***,we introduce a silicon photonic MEMS platform consisting of high-performance nano-opto-electromechanical devices fully integrated alongside standard silicon photonics foundry components,with wafer-level sealing for long-term reliability,flip-chip bonding to redistribution interposers,and fibre-array attachment for high port count optical and electrical *** experimental demonstration of fundamental silicon photonic MEMS circuit elements,including power couplers,phase shifters and wavelength-division multiplexing devices using standardized technology lifts previous impediments to enable scaling to very large photonic integrated circuits for applications in telecommunications,neuromorphic computing,sensing,programmable photonics,and quantum computing.

关键词： tuning sealing photonics