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The Transcriptional Activity of LAMP3 Gene Involved in Autophagocytosis in Colorectal Cancer LAMP3 Expression in Colorectal Cancer

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Journal of Biosciences and Medicines 2017年第12期5卷 24-36页

作者： Martyna Bednarczyk malgorzata muc-wierzgon Nikola Zmarzly Celina Kruszniewska-Rajs Beniamin Grabarek Agata Kazmierczak Bartlomiej Skowronek Klaudia Simka Dariusz Waniczek Katarzyna Walkiewicz Urszula Mazurek Department of Internal Medicine School of Public Health in BytomMedical University of SilesiaKatowicePoland Department of Molecular Biology School of Pharmacy with the Division of Laboratory Medicine in SosnowiecMedical University of SilesiaKatowicePoland Chair of General Colorectal and Polytrauma SurgeryDepartment of Propaedeutics SurgeryMedical University of SilesiaKatowicePoland

Background: Autophagocytosis is a biological process involving the controlled distribution of cell fragments and organelles in order to obtain an additional source of energy. LAMP3 (lysosome-associated membrane protein 3) is a heavily glycosylated integral membrane protein located mainly in the lysosomal membrane. Recent studies have shown that it participates in tumor metastasis and drug resistance. Its main role is contribution to tumor cells proliferation, migration and invasion. The aim of this study was to determine changes in transcriptional activity of LAMP3 at various stages of colorectal cancer. In addition, an attempt was made to select miRNAs potentially regulating LAMP3 expression using bioinformatic databases. Methods: The study was conducted on healthy colon samples and colon cancer samples in four stages. Molecular analysis included the extraction of total RNA, purification of the obtained extracts, expression profile analysis using oligonucleotide microarray technique and in silico determination of miRNAs potentially regulating the LAMP3 expression. Results: The level of LAMP3 expression is higher in colorectal adenocarcinoma cells than in non-cancerous cells and depends on the stage of the disease. Conclusions: LAMP3 may promote cancer progression, metastasis and cause the resistance to treatment.

关键词： Autophagy Colorectal Cancer LAMP3 Microarrays miRNA

Specific metabolic biomarkers as risk and prognostic factors in colorectal cancer

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World Journal of Gastroenterology 2014年第29期20卷 9759-9774页

作者： Magorzata muc-Wierzgoń Ewa Nowakowska-Zajdel Sylwia Dziegielewska-Gesiak Teresa Kokot Katarzyna Klakla Edyta Fatyga Elzbieta Grochowska-Niedworok Dariusz Waniczek Janusz Wierzgoń Department of Internal Diseases Medical University of Silesia Department of Human Nutrition Medical University of Silesia Department of Gastrointestinal Surgery Medical University of Silesia Department of Oncological and Reconstructive Surgery Center of Oncology Maria Skodowska-Curie Memorial Institute in Warsaw

Advances in genomics,molecular pathology and metabolism have generated many candidate biomarkers of colorectal cancer with potential clinical *** and biological studies suggest a role for adiposity,dyslipidaemia,hyperinsulinemia,altered glucose homeostasis,and elevated expression of insulin-like growth factor(IGF)axis members in the risk and prognosis of *** review discusses some recent past and current approaches being taken by researches in obesity and metabolic *** authors describe three main systems as the most studied metabolic candidates of carcinogenesis:dyslipidemias,adipokines and insulin/IGF ***,each of these components is unsuccessful in defining the diseases risk and progression,while their co-occurrence increases cancer incidence and mortality in both men and women.

关键词： Colorectal cancer Metabolic biomarkers Risk Prognosis Dyslipidemias Adipokines Insulin-like growth factor-system

Differential expression of mucin 1 and mucin 2 in colorectal cancer

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World Journal of Gastroenterology 2018年第36期24卷 4164-4177页

作者： Aldona Kasprzak Elzbieta Siodla malgorzata Andrzejewska Jacek Szmeja Agnieszka Seraszek-Jaros Szczepan Cofta Witold Szaflarski Department of Histology and Embryology Poznan University of Medical SciencesPoznan 60-781Poland Chair and Department of General Surgery Endocrinological and Gastroenterological OncologyPoznan University of Medical SciencesPoznan 60-355Poland Department of Bioinformatics and Computational Biology Chair of Clinical PathomorphologyPoznan University of Medical SciencesPoznan 60-529Poland Department of Pulmonology Allergology and Respiratory OncologyPoznan University of Medical SciencePoznan 60-569Poland

AIM To determine tissue expression(mRNA, protein) of two types of mucins [mucin 1(muc1) and mucin 2(muc2)] in patients with colorectal cancer(CRC).METHODS Expression of membrane-bound mucin(muc1) and secretory mucin(muc2) in CRC(mRNA, protein) were analyzed in tissue material including fragments of tumorsobtained from CRC patients(n = 34), and fragments of normal colorectal tissue from the same patients(control). The analysis was conducted using real-time quantitative polymerase chain reaction(RT-qPCR)(transcripts), immunohistochemistry(IHC)(apomucins), and the modern approach for morphometric analysis of IHC reaction(HSV filter software). Results on tissue expression of both mucins(mRNA, protein) were compared to histological alterations in colorectal cancer samples and correlated with selected clinical data in the patients. The statistical analysis was conducted using Statistica PL v. 12.0 *** Significantly higher expression of the muc1 mRNA in the CRC, compared with the control and the borderline correlation of mRNA expression with muc1 protein levels in colorectal samples was observed. The expression of apomucins concerned cell membranes(muc1) and cytoplasm(muc2) and occurred both in control tissues and in most cancerous samples. There were no significant relationships between muc1(mRNA, protein) and the clinicopathological data of patients. muc2 protein expression was significantly lower as compared to the control, while muc2 mRNA expression was comparable in both groups. The muc1/muc2 ratio was significantly higher in CRC tissues than in the control. The higher expression of muc2 was a feature of mucinous CRC subtypes, and characterized higher histological stage of tumors. Negative correlations have been obtained between muc2 and the Ki-67 antigen, as well as between muc2 and p53 protein expressions in *** A combination of tissue overexpression of muc1, reduced muc2 expression, and high ratio of muc1/muc2 is a factor of poor prognosis i

关键词： mucins Real-time quantitative polymerase chain reaction Colorectal cancer Immunohistochemistry HSV filter program

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将大数据转化为大价值的10种途径

电脑知识与技术（经验技巧） 2018年第6期 107-110页

作者： muc

大数据可以产生很多价值,但前提是只有当企业真正知道如何充分利用这些大数据的时候。当前,大数据已经登上了历史舞台。在全球范围内,拥有超过半数的企业组织都已经将大数据项目视为其未来发展的机遇,并计划在未来几年内进一步增加对大... 详细信息

大数据可以产生很多价值,但前提是只有当企业真正知道如何充分利用这些大数据的时候。当前,大数据已经登上了历史舞台。在全球范围内,拥有超过半数的企业组织都已经将大数据项目视为其未来发展的机遇,并计划在未来几年内进一步增加对大数据项目的投资。但是,大数据的价值并不仅仅是来自对于相关数据信息的收集而已,这仅仅是起点。大数据的真正价值来自企业组织利用所存储的信息以发现新的洞察分析见解的能力,然后从中提取出有用的价值。

关键词：价值数据转化企业组织数据项目数据信息组织利用全球范围

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多云发展的5大驱动因素

电脑知识与技术（经验技巧） 2018年第6期 105-106页

作者： muc

越来越多的企业选择公有云,软件即服务（SaaS）和基础设施的组合来实现业务创新并支持其应用程序和业务模型。多云（Multicloud）环境可以解决企业更广泛的业务目标,它让企业能够使用更有价格优势的云服务,利用特定云服务提供商提供的... 详细信息

越来越多的企业选择公有云,软件即服务（SaaS）和基础设施的组合来实现业务创新并支持其应用程序和业务模型。多云（Multicloud）环境可以解决企业更广泛的业务目标,它让企业能够使用更有价格优势的云服务,利用特定云服务提供商提供的速度、容量和功能,或是特定垂直市场中的云甚至是私有云。有很多因素在推动企业采用多云环境,以下将介绍5大驱动因素：一、加速云端创新新兴技术是多云环境采用的主要推动因素,我们目睹了顶级云提供商。

关键词：驱动因素服务提供商业务模型业务创新应用程序基础设施价格优势企业

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西南地区农村留守儿童监护协作问题研究——以S省三县“童伴计划”为切入点

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预防青少年犯罪研究 2022年第1期 77-85页

作者：徐爽中央民族大学农村留守儿童监护协作研究项目组中央民族大学法学院不详

留守儿童长期处于隔代监护状态下,其抚养教育等基本权利的实现,不光需要家庭担责,也需要政府、学校和社会力量的支持协同。《民法典》和新修订《未成年人保护法》《家庭教育促进法》等对监护职责协作作出了明确规定。项目组选取S省中农... 详细信息

留守儿童长期处于隔代监护状态下,其抚养教育等基本权利的实现,不光需要家庭担责,也需要政府、学校和社会力量的支持协同。《民法典》和新修订《未成年人保护法》《家庭教育促进法》等对监护职责协作作出了明确规定。项目组选取S省中农村留守儿童较多的三个县城,以儿童关爱监护协作项目“童伴计划”为切入点,运用CIPP模型开展调研,考察农村留守儿童监护职责协作模式的实际运行状况,针对异地搬迁的留守儿童家庭“返乡”流动现象、国家通用语言文字普及率不足等问题,建议加强对农村留守儿童家庭的全过程监督,推进隔代家长教育,加强社会力量的多元引入,建设“童伴计划”标准化体系,从而完善农村留守儿童监护协作制度,切实保障其基本权利。

关键词：未成年人保护法留守儿童监护协作童伴计划

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阴茎胶样变性

世界核心医学期刊文摘（皮肤病学分册） 2006年第10期2卷 39-39页

作者： Preston P.W. Orpin S.D. muc R. Zaki I. 任建文 Department of Dermatology Solihull HospitalHeart ofEngland NHS TrustLode LaneSolihullWest MidlandsB91 2JLUnited Kingdom Dr.

Colloid degeneration is a rare but well-recognized entity,usually occurring on chronically sun-exposed *** report acase occurring on penile skin with no history of such exposure.

关键词：阴茎男生殖器

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