检索结果-南通市图书馆

Molecular and functional characterization of the American cockroach, Periplaneta americana, Rab5： the first exopterygotan low molecular weight ovarian GTPase during oogenesis

引用

Insect Science 2018年第5期25卷 751-764页

作者： Mohamed Elmogy Amr A. Mohamed Muhammad Tufail Tomohide Uno makio takeda Department of Entomology Faculty of Science Cairo University Orman Giza Egypt Department of Plant Protection College of Food and Agriculture Sciences King Saud University Riyadh Saudi Arabia Graduate School of Agricultural Science Kobe University Nada- ku Kobe Japan Laboratory of Biological Chemistry Faculty of Agriculture Department of Biofuncfional Chemistry Kobe University Nada-ku Hyogo Japan

The small Rab GTPases are key regulators of membrane vesicle trafficking. Ovaries of Periplaneta americana （Linnaeus）（Blattodea： Blattidae） have small molecular weight GTP/ATP-binding proteins during early and late vitellogenic periods of oogenesis. However, the identification and characterization of the detected proteins have not been yet reported. Herein, we cloned a cDNA encoding Rab5 from the American cockroach, P. americana, ovaries （PamRab5）. It comprises 796 bp, encoding a protein of 213 amino acid residues with a predicted molecular weight of 23.5 kDa. PamRab5 exists as a single-copy gene in the P. americana genome, as revealed by Southern blot analysis. An approximate 2.6 kb ovarian mRNA was transcribed especially at high levels in the previtellogenic ovaries, detected by Northern blot analysis. The muscle and head tissues also showed high levels of PamRab5 transcript. PamRab5 protein was localized, via immunofluorescence labeling, to germline-derived cells of the oocytes, very early during oocyte differentiation. Immunoblotting detected a ~25 kDa signal as a membrane-associated form revealed after application of detergent in the extraction buffer, and 23 kDa as a cytosolic form consistent with the predicted molecular weight from amino acid sequence in different tissues including ovary, muscles and head. The PamRab5 during late vitellogenic periods is required to regulate the endocytotic machinery during oogenesis in this cockroach. This is the first report on Rab5 from a hemimetabolan, and presents an inaugural step in probing the molecular premises of insect oocyte endocytotic trafficking important for oogenesis and embryonic development.

关键词： endocytosis membrane trafficking oogenesis Periplaneta americana Rab5-GTPase

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

光照对蟋蟀Gryllus varius(Orthoptera：Gryllidae)的若虫活动、发育及新陈代谢的影响(英文)

引用

应用昆虫学报 2015年第2期52卷 440-445页

作者：李晓婷 takeda makio 日本神户大学农学研究科神户6578501

【目的】测试光照对蟋蟀Gryllus varius(Orthoptera:Gryllidae)行为和发育的调节,包括对活动水平、发育时间、体长以及饮食的影响。【方法】通过摄像机监控蟋蟀若虫的行为活动进行观察并对其行为进行分析研究。【结果】与长光照(16L︰8D... 详细信息

【目的】测试光照对蟋蟀Gryllus varius(Orthoptera:Gryllidae)行为和发育的调节,包括对活动水平、发育时间、体长以及饮食的影响。【方法】通过摄像机监控蟋蟀若虫的行为活动进行观察并对其行为进行分析研究。【结果】与长光照(16L︰8D)相比,在短光照(12L︰12D)下,蟋蟀若虫发育时间长,个体大,但是活动和排泄物都比较少。结果表明,在长光照下,若虫的代谢速率快。两种不同光照的影响结果差异性显著。此外,研究结果表明体型较大的生物存活时间长,但是代谢速率慢并且活动水平低。【结论】光周期显著影响蟋蟀若虫的发育。活动水平、发育时间、个体大小和代谢速率之间存在着一定的联系,体型较大的个体存活时间长,但是代谢速率慢并且活动水平低;反之,体型小的个体存活时间短,代谢速率快并且活动水平高。

关键词：光周期活动发育时间体长代谢速率蟋蟀

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

中国蝼蛄属研究及一新种记述(直翅目,蝼蛄科)(英文)

引用

动物分类学报 2008年第1期33卷 14-17页

作者：马丽滨许升全竹田真木生陕西师范大学动物研究所西安710062 神户大学昆虫科学研究室日本神户6578501

给出了中国蝼蛄属的检索表,并描述了该属1新种*** ***.。新种与尼泊尔种类***相似,但可以通过如下特征加以区分:径脉末端不分岔,翅室呈三角形,阳茎基片横向骨片的侧端尖锐;此新种还与河南蝼蛄***相似,区别为:新种前翅超过第5节背板,后... 详细信息

给出了中国蝼蛄属的检索表,并描述了该属1新种*** ***.。新种与尼泊尔种类***相似,但可以通过如下特征加以区分:径脉末端不分岔,翅室呈三角形,阳茎基片横向骨片的侧端尖锐;此新种还与河南蝼蛄***相似,区别为:新种前翅超过第5节背板,后翅到达第4节背板后缘,而河南蝼蛄***前后翅均未伸达腹部第4节背板后缘;新种前胸背板无心纹斑,河南蝼蛄***有;新种阳茎侧突囊弯钩状,后者为弯月形。

关键词：中国蝼蛄属蝼蛄科新种直翅目

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Isolation and Characterization of SARS-CoV-2 in Kenya

引用

American Journal of Molecular Biology 2024年第2期14卷 66-83页

作者： Albina makio Robinson Mugasiali Irekwa Matthew Mutinda Munyao Caroline Wangui Njoroge Peter Kipkemboi Rotich Tonny Teya Nyandwaro Joanne Jepkemei Yego Anne Wanjiru Mwangi James Hungo Kimotho Ronald Tanui Vincent Rutto Samson Muuo Nzou Center for Traditional Medicine and Drug Research Kenya Medical Research Institute Nairobi Kenya Center for Microbiology Research Kenya Medical Research Institute Nairobi Kenya Production Department Kenya Medical Research Institute Nairobi Kenya Department of Biochemistry and Microbiology Rhodes University Grahamstown South Africa Center for Virus Research Kenya Medical Research Institute Nairobi Kenya

The discovery of Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) in Wuhan, Hubei province, China, in December 2019 raised global health warnings. Quickly, in 2020, the virus crossed borders and infected individuals across the world, evolving into the COVID-19 pandemic. Notably, early signs of the virus’s existence were observed in various countries before the initial outbreak in Wuhan. As of 12th of April, the respiratory disease had infected over 762 million people worldwide, with over 6.8 million deaths recorded. This has led scientists to focus their efforts on understanding the virus to develop effective means to diagnose, treat, prevent, and control this pandemic. One of the areas of focus is the isolation of this virus, which plays a crucial role in understanding the viral dynamics in the laboratory. In this study, we report the isolation and detection of locally circulating SARS-CoV-2 in Kenya. The isolates were cultured on Vero Cercopithecus cell line (CCL-81) cells, RNA extraction was conducted from the supernatants, and reverse transcriptase-polymerase chain reaction (RT-PCR). Genome sequencing was done to profile the strains phylogenetically and identify novel and previously reported mutations. Vero CCL-81 cells were able to support the growth of SARS-CoV-2 in vitro, and mutations were detected from the two isolates sequenced (001 and 002). Genome sequencing revealed the circulation of two isolates that share a close relationship with the Benin isolate with the D614G common mutation identified along the S protein. These virus isolates will be expanded and made available to the Kenya Ministry of Health and other research institutions to advance SARS-CoV-2 research in Kenya and the region.

关键词： SARS-CoV-2 COVID-19 Whole Genome Sequencing Phylogenetic Analysis Nucleotide Substitutions Amino Acid Changes

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fabrication and modelling of Si_(3)N_(4)ceramics with radial grain alignment generated through centripetal sinter-forging

引用

Journal of Materials Science & Technology 2022年第31期126卷 1-14页

作者： Da-Wang Tan Zhen-Yong Lao Wei-Ming Guo Akira Kondo Takahiro Kozawa makio Naito Kevin Plucknett Hua-Tay Lin Department of Electromechanical Engineering Guangdong University of TechnologyGuangzhou 510006China Joining and Welding Research Institute Osaka University11-1 MihogaokaIbarakiOsaka 567-0047Japan Department of Mechanical Engineering Dalhousie UniversityHalifaxNova Scotia B3H 4R2Canada

Silicon nitride(Si_(3)N_(4))based ceramics are one of the most attractive advanced engineering materials,which have been widely used under high-speed rotational operation or for mechanical contacts across a curved *** the present study,rotationally symmetric texturing of Si_(3)N_(4),with radial grain align-ment,was obtained by centripetal sinter-forging(CSF)of a partially sintered *** average values of the included angles between the c-axis of the local Si_(3)N_(4)grain and radial direction were approxi-mately 16.4°and 11.0°,on the section plane perpendicular to the pressing direction,and parallel to both the pressing and radial directions,*** compressive strain in the pressing direction forced the ceramic body to flow towards the central axis,resulting in compressive strain in the tangential di-rection and tensile strain in the radial direction.A fundamental physical model was created to simulate the grain rotation during the 3-dimentional strain reorientation,which revealed the rod-like grain would preferentially rotate toward the center of the sample under the CSF *** addition,due to the fric-tion between the sample surface and the pressing punch,the increased shear strain could enhance the Si_(3)N_(4)grain ***,ceramics with rod-like grains perpendicular to the curved side sur-face could be anticipated by applying the centripetal forming concept in a controlled manner.

关键词： Si_(3)N_(4)ceramic Texture Radial grain alignment Modeling CSF

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于机械干态颗粒涂层技术制备cBN@TiN粉体

引用

机械工程材料 2024年第3期48卷 37-42页

作者：罗展鹏刘润平郭伟明谭大旺罗嗣春林华泰内藤牧男广东工业大学机电工程学院广州510006 岭南师范学院机电工程学院湛江524048 萍乡学院机械电子工程学院萍乡337055 大阪大学接合科学研究所大阪565-0871

采用机械干态颗粒涂层技术分别制备了cBN@TiO_(2)(TiO_(2)包覆立方氮化硼)粉体和cBN@(TiO_(2)+C)(TiO_(2)+碳包覆立方氮化硼)粉体,然后在1600℃、N_(2)气氛下进行高温热处理制备cBN@TiN(TiN包覆立方氮化硼)粉体,研究了高温热处理前后粉... 详细信息

采用机械干态颗粒涂层技术分别制备了cBN@TiO_(2)(TiO_(2)包覆立方氮化硼)粉体和cBN@(TiO_(2)+C)(TiO_(2)+碳包覆立方氮化硼)粉体,然后在1600℃、N_(2)气氛下进行高温热处理制备cBN@TiN(TiN包覆立方氮化硼)粉体,研究了高温热处理前后粉体的物相组成与微观形貌以及高温反应机理。结果表明:机械干态颗粒涂层技术可以使纳米TiO_(2)和纳米TiO_(2)+C颗粒均匀包裹在cBN颗粒表面。在高温热处理过程中,TiO_(2)与cBN反应生成液相B_(2)O_(3),促进了cBN相变成六方氮化硼(hBN),cBN的高温稳定性差,以cBN@TiO_(2)为原料制备的cBN@TiN粉体颗粒表面形成由TiN相和hBN相组成的片状结构层;当存在碳时,TiO_(2)会优先与碳发生还原反应生成TiN,抑制B_(2)O_(3)的生成,从而降低cBN相变量,此时cBN的高温稳定性好,以cBN@(TiO_(2)+C)为原料制备的cBN@TiN粉体颗粒表面形成主要为TiN相的颗粒结构层。

关键词： TiN包覆cBN粉体高温热处理机械干态颗粒涂层技术热稳定性

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chenguodaite (Ag_9FeTe_2S_4):a new tellurosulfide mineral from the gold district of East Shandong Peninsula,China

引用

Chinese Science Bulletin 2008年第22期53卷 3567-3573页

作者： GU XiangPing Makoto WATANABE XIE XianDe PENG ShengLin Yoshihiro NAKAMUTA makio OHKAWA Kenichi HOSHINO Kazumasa OHSUMI Yasuhiro SHIBATA Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640 China Department of Earth & Planetary Sciences Hiroshima University Higashi Hiroshima City Hiroshima 739-8526 Japan Department of Earth Sciences and Environmental Engineering Central South University Changsha 410083 China The Kyushu University Museum Kyushu University Kakozaki Fukuoka 812-8581 Japan Photon Factory National Laboratory for High Energy Physics (KEK) Oho 1-1 Tsukuba Ibaraki 305 Japan

Chenguodaite, approved by IMA-CNMMN (2004-042a), was discovered in the Bunan quartz vein-type gold deposit in the gold district of East Shandong Peninsula. The mineral occurs in high grade Au-Ag-Cu ores, coexisting with galena, chalcopyrite, hessite, electrum, unnamed Ag6TeS2 and Ag16FeBiTe3S8, enclosed and replaced by native silver and acanthite. In the reflected light microscope, the mineral has light gray color, indistinguishable anistropism and hardness around 2―3. The color indices of chenguodaite relative to ICE C illuminator are: x=0.3027, y=0.3076, Y=25.78%, λd=474 nm, Pe=3.68%, similar to those of canfieldite. The average chemical composition from 16 microprobe analyses is Ag8.97Fe1.00Te1.99S4.04, idealized to Ag9FeTe2S4. The polycrystalline X-ray diffraction of chenguodaite by Gandolfi camera and synchrotron oscillation photography results in 67 reflections with the 12 strongest being (relative intensity in bracket): 6.742(69), 6.416(39), 5.951(33), 3.265(100), 2.981(24), 2.649(22), 2.25(24), 2.188(71), 2.142(22), 2.123(31), 2.044(23), 1.949(33), which are indexed to a primitive orthorhombic cell with a=12.769 (2) , b= 14.814(2), c= 16.233 (1) , V= 3070.63, Z = 9, Dcal.=6.85 g/cm3. The name is for the late Prof. Chen Guoda, a famous Chinese geologist and the founder of Diwa-Geodepression theory of tectonics.

关键词： Ag9FeTe2S4 新矿石硫化亚碲山东东部

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Femoral Hernia: A Review of the Clinical Anatomy and Surgical Treatment

引用

Surgical Science 2013年第10期4卷 453-458页

作者： makio Mike Nobuyasu Kano Department of Surgery Kameda Medical Center Kamogawa Japan

Purpose: Femoral hernia is a kind of ventral hernia that surgeons commonly encounter, second in frequency only to inguinal hernia. Femoral hernias often require emergency surgery because of incarceration or strangulation of the intestine. In addition, intestinal resection may need to be considered based on intestinal viability. Definitive preoperative diagnosis and strategic planning for surgery are thus important. The surgeon should consider the operation in the context of the clinical anatomy of the abdominal cavity. Therefore the essence of the clinical anatomy and treatment of femoral hernia is described. Methods: The medical records of 38 patients who underwent femoral hernia repair between March 2006 and November 2011 were retrospectively analyzed. Results: Femoral hernioplasty was performed with original mesh repair or Ruggi’s repair plus iliopubic tract repair (or Bassini’s repair). The mean patient age was 76.7 years, and a female predominance was apparent. Twenty-four patients underwent emergency surgery with a diagnosis of incarcerated femoral hernia. Nine patients showed intestinal strangulation and underwent resection of the small intestine. Four patients developed complications. One patient died due to aspiration pneumonia. No recurrences were encountered after 6 months to 6 years of follow-up. Conclusion: Femoral hernia is an important surgical condition with high rates of incarceration/strangulation and intestinal resection. Correct preoperative diagnosis of femoral hernia and a strict operative strategy are important. The original mesh repair is effective and easy to perform.

关键词： Femoral Hernia Clinical Anatomy Mesh Repair Ruggi’s Repair

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Erythropoietin -induced proliferation of gastric mucosal cells

引用

World Journal of Gastroenterology 2006年第2期12卷 234-239页

作者： Kazuro Itoh Yoshio Sawasaki Kyoko Takeuchi Shingo Kato Nobuhiro Imai Yoichiro Kato Noriyuki Shibata makio Kobayashi Yoshiyuki Moriguchi Masato Higuchi Fumio Ishihata Yushi Sudoh Soichiro Miura Second Department of Internal Medicine National Defense Medical CollegeTokorozawaSaitamaJapan First Department of Anatomy National Defense Medical CollegeTokorozawaSaitamaJapan Department of Pathology Tokyo Women's Medical CollegeShinjuku-kuTokyoJapan Chugai Pharmaceutical Co. Ltd.GotenbaShizuokaJapan Kisen Hospital Katsushika-kuTokyoJapan

AIM： To analyze the localization of erythropoietin receptor on gastric specimens and characterize the effects of erythropoietin on the normal gastric epithelial proliferation using a porcine gastric epithelial cell culture model. METHODS： Erythropoietin receptor was detected by RT-PCR, Western blotting and immunohistochermistry. Growth stimulation effects of erythropoietin on cultured gastric mucosal cells were determined by ELISA using bromodeoxyuridine （BrdU）. RESULTS： Erythropoietin receptor was detected on cultured porcine gastric mucosal epithelial cells. Erythropoietin receptor was also detected histochemically at the base of gastric mucosal epithelium. BrdU assay demonstrated a dose-dependent increase in growth potential of cultured porcine gastric mucosal epithelial cells by administration of erythropoietin, as well as these effects were inhibited by administration of antierythropoietin antibody （P〈 0.01）. CONCLUSION： These findings indicate that erythropoietin has a potential to proliferate gastric mucosal epithelium via erythropoietin receptor.

关键词： Erythropoietin Erythropoietin receptor Gastric epithelial cell proliferation Porcine gastric mucosal epithelial cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Dynamic Molecular Behavior and Cluster Structure of Octanoic Acid in Its Liquid and CCl<sub>4</sub>Solution

引用

Food and Nutrition Sciences 2013年第9期4卷 25-32页

作者： Hideyo Matsuzawa Masaya Tsuda Hideyuki Minami makio Iwahashi Department of Chemistry School of Science Kitasato University Sagamihara Japan.

Fatty acids such as oleic and stearic acids having a long hydrocarbon chain are known to exist as dimers in their melt and even in a non-polar solvent. In their melt the dimers arrange longitudinally and alternately to form clusters which resemble a smectic liquid crystal. The clusters determine the liquid properties of the fatty acids such as density, viscosity and fluidity. Then, do the dimers of fatty acid having a moderate-length hydrocarbon chain construct such the clusters? In the present study the dynamic molecular behavior and assembly structure of octanoic acid in its melt and also in CCl4 solution have been investigated by the X-ray diffraction, near infrared spectroscopy, 1H-NMR chemical shift, self-diffusion coefficient and 13C-NMR spin-lattice relaxation time measurements. From these results it has been revealed that the clusters of octanoic acid exist in its melt and also in CCl4 and that the clusters in the melt disintegrate with an increase in temperature. The dissociation profile of dimers of octanoic acid into monomers in CCl4 also has been clarified.

关键词： Octanoic Acid Oleic Acid Stearic Acid X-Ray Diffraction Near-Infrared Spectroscopy Self-Diffusion Coefficient 13C-NMR Spin-Lattice Relaxation Time 1H-NMR Chemical Shift

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：